P0ACZ2 (ETP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low molecular weight protein-tyrosine-phosphatase etp
EC=3.1.3.48

Gene names

Name:	etp
Synonyms:	yccY
Ordered Locus Names:	b0982, JW5132

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates etk.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Caution	In E.coli K12 / MG1655 and K12 / W3110 this operon is silenced by an IS1D insertion in the promoter region.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	protein tyrosine phosphatase activity Inferred from direct assay PubMed 12657060. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Low molecular weight protein-tyrosine-phosphatase etp		PRO_0000046576
Sites
	Active site	13	1	Nucleophile By similarity
	Active site	18	1	By similarity
	Active site	119	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ACZ2 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 136DA7AEF6AE8F0C	FASTA	148	16,386
	10 20 30 40 50 60 MAQLKFNSIL VVCTGNICRS PIGERLLRKR LPGVKVKSAG VHGLVKHPAD ATAADVAANH 70 80 90 100 110 120 GVSLEGHAGR KLTAEMARNY DLILAMESEH IAQVTAIAPE VRGKTMLFGQ WLEQKEIPDP 130 140 YRKSQDAFEH VYGMLERASQ EWAKRLSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria." Vincent C., Duclos B., Grangeasse C., Vaganay E., Riberty M., Cozzone A.J., Doublet P. J. Mol. Biol. 304:311-321(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JM109 / ATCC 53323.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74067.2. AP009048 Genomic DNA. Translation: BAA35747.2.
PIR	D64839.
RefSeq	NP_415502.4. NC_000913.2. YP_489253.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACZ2.
SMR	P0ACZ2. Positions 4-146.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0982.
Protein family/group databases
PptaseDB	P3D0412163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74067; AAC74067; b0982. BAA35747; BAA35747; BAA35747.
GeneID	12932413. 945236.
KEGG	ecj:Y75_p0953. eco:b0982.
PATRIC	32117187. VBIEscCol129921_1017.
Organism-specific databases
EchoBASE	EB3491.
EcoGene	EG13727. etp.
Phylogenomic databases
eggNOG	COG0394.
HOGENOM	HOG000273091.
KO	K01104.
OMA	VMEKKHI.
ProtClustDB	PRK11391.
Enzyme and pathway databases
BioCyc	EcoCyc:G6503-MONOMER. ECOL316407:JW5132-MONOMER. MetaCyc:G6503-MONOMER.
Gene expression databases
Genevestigator	P0ACZ2.
Family and domain databases
InterPro	IPR023485. Ptyr_pPase_SF. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. PTHR11717. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
SUPFAM	SSF52788. Tyr_Pase_low_mol_wt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ETP_ECOLI

Accession

Primary (citable) accession number: P0ACZ2
Secondary accession number(s): P75880, Q8XC24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program