Reviewed, UniProtKB/Swiss-Prot P0ACY1 (YDJA_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative NAD(P)H nitroreductase ydjA
    EC=1.-.-.-

Gene names

Name:	ydjA
Ordered Locus Names:	b1765, JW1754

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 FMN per subunit. Ref.6
Subunit structure	Homodimer. Ref.6
Sequence similarities	Belongs to the nitroreductase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Putative NAD(P)H nitroreductase ydjA

PRO_0000169002

Regions

Nucleotide binding

10 – 12

3

FMN

Nucleotide binding

121 – 126

6

NAD or NADP Potential

Nucleotide binding

131 – 133

3

FMN

Sites

Binding site

35

1

FMN; shared with dimeric partner

Binding site

39

1

FMN; shared with dimeric partner

Secondary structure

1

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183

Helix Strand Turn

Details...

Helix

3 – 9

7

Beta strand

16 – 18

3

Helix

23 – 33

11

Beta strand

46 – 51

6

Helix

53 – 69

17

Helix

73 – 81

9

Helix

82 – 85

4

Beta strand

86 – 94

9

Beta strand

100 – 102

3

Helix

104 – 123

20

Turn

124 – 126

3

Beta strand

128 – 132

5

Helix

135 – 138

4

Helix

140 – 145

6

Beta strand

153 – 163

11

Helix

177 – 179

3

Beta strand

180 – 182

3

Sequences

	Sequence		Length	Mass (Da)
	P0ACY1-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: A69809EE378847CB	FASTA	183	20,059
	10 20 30 40 50 60 MDALELLINR RSASRLAEPA PTGEQLQNIL RAGMRAPDHK SMQPWHFFVI EGEGRERFSA 70 80 90 100 110 120 VLEQGAIAAG SDDKAIDKAR NAPFRAPLII TVVAKCEENH KVPRWEQEMS AGCAVMAMQM 130 140 150 160 170 180 AAVAQGFGGI WRSGALTESP VVREAFGCRE QDKIVGFLYL GTPQLKASTS INVPDPTPFV TYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme." Sawers G., Heider J., Zehelein E., Boeck A. J. Bacteriol. 173:4983-4993(1991) [PubMed: 1650339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Crystal structure of a minimal nitroreductase, ydjA, from Escherichia coli K12 with and without FMN cofactor." Choi J.-W., Lee J., Nishi K., Kim Y.-S., Jung C.-H., Kim J.-S. J. Mol. Biol. 377:258-267(2008) [PubMed: 18241886] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN, COFACTOR, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M68961 Genomic DNA. Translation: AAA24245.1.
U00096 Genomic DNA. Translation: AAC74835.1.
AP009048 Genomic DNA. Translation: BAA15556.1.

PIR

A40360.

RefSeq

AP_002384.1.
NP_416279.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BM1	X-ray	2.00	A/B	1-183	[»]
3BM2	X-ray	2.10	A/B	1-183	[»]

ModBase

Search...

Genome annotation databases

GeneID

945964.

GenomeReviews

Gene locus JW1754 in contig AP009048_GR.
Gene locus b1765 in contig U00096_GR.

KEGG

ecj:JW1754.
eco:b1765.

Organism-specific databases

EchoBASE

EB1124.

EcoGene

EG11134. ydjA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0ACY1.

OMA

P0ACY1. QIVGFLY.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11134-MON.

Family and domain databases

InterPro

IPR000415. Nitroreductase-like.
[Graphical view]

Pfam

PF00881. Nitroreductase. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YDJA_ECOLI

Accession

Primary (citable) accession number: P0ACY1
Secondary accession number(s): P24250

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)