Putative NAD(P)H nitroreductase ydjA - Escherichia coli (strain K12)

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P0ACY1 (YDJA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative NAD(P)H nitroreductase ydjA
EC=1.-.-.-

Gene names

Name:	ydjA
Ordered Locus Names:	b1765, JW1754

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 FMN per subunit. Ref.6
Subunit structure	Homodimer. Ref.6
Sequence similarities	Belongs to the nitroreductase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from direct assay Ref.6. Source: EcoCyc oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Putative NAD(P)H nitroreductase ydjA

PRO_0000169002

Regions

Nucleotide binding

10 – 12

FMN

Nucleotide binding

121 – 126

NAD or NADP Potential

Nucleotide binding

131 – 133

FMN

Sites

Binding site

FMN; shared with dimeric partner

Binding site

FMN; shared with dimeric partner

Secondary structure

183

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ACY1 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: A69809EE378847CB
Show »

FASTA

183

20,059

        10         20         30         40         50         60 
MDALELLINR RSASRLAEPA PTGEQLQNIL RAGMRAPDHK SMQPWHFFVI EGEGRERFSA 

        70         80         90        100        110        120 
VLEQGAIAAG SDDKAIDKAR NAPFRAPLII TVVAKCEENH KVPRWEQEMS AGCAVMAMQM 

       130        140        150        160        170        180 
AAVAQGFGGI WRSGALTESP VVREAFGCRE QDKIVGFLYL GTPQLKASTS INVPDPTPFV 


TYF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme." Sawers G., Heider J., Zehelein E., Boeck A. J. Bacteriol. 173:4983-4993(1991) [PubMed: 1650339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
[6]	"Crystal structure of a minimal nitroreductase, ydjA, from Escherichia coli K12 with and without FMN cofactor." Choi J.-W., Lee J., Nishi K., Kim Y.-S., Jung C.-H., Kim J.-S. J. Mol. Biol. 377:258-267(2008) [PubMed: 18241886] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN, COFACTOR, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M68961 Genomic DNA. Translation: AAA24245.1.
U00096 Genomic DNA. Translation: AAC74835.1.
AP009048 Genomic DNA. Translation: BAA15556.1.

PIR

A40360.

RefSeq

NP_416279.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BM1	X-ray	2.00	A/B	1-183	[»]
3BM2	X-ray	2.10	A/B	1-183	[»]

ProteinModelPortal

P0ACY1.

SMR

P0ACY1. Positions 1-183.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31841N.

IntAct

P0ACY1. 11 interactions.

MINT

MINT-1239401.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000798; EBESCP00000000798; EBESCG00000000667.
EBESCT00000017722; EBESCP00000017013; EBESCG00000016778.

GeneID

945964.

GenomeReviews

Gene locus JW1754 in contig AP009048_GR.
Gene locus b1765 in contig U00096_GR.

KEGG

ecj:JW1754.
eco:b1765.

PATRIC

32118841. VBIEscCol129921_1838.

Organism-specific databases

EchoBASE

EB1124.

EcoGene

EG11134. ydjA.

Phylogenomic databases

eggNOG

COG0778.

GeneTree

EBGT00050000010740.

HOGENOM

HBG751302.

OMA

LNRVSVP.

PhylomeDB

P0ACY1.

ProtClustDB

PRK10828.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11134-MONOMER.

Gene expression databases

Genevestigator

P0ACY1.

Family and domain databases

InterPro

IPR000415. Nitroreductase-like.
[Graphical view]

Gene3D

G3DSA:3.40.109.10. G3DSA:3.40.109.10. 1 hit.

Pfam

PF00881. Nitroreductase. 1 hit.
[Graphical view]

SUPFAM

SSF55469. Nitroreductase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

YDJA_ECOLI

Accession

Primary (citable) accession number: P0ACY1
Secondary accession number(s): P24250

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents