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P0ACY1 (YDJA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative NAD(P)H nitroreductase ydjA

EC=1.-.-.-
Gene names
Name:ydjA
Ordered Locus Names:b1765, JW1754
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Cofactor

Binds 1 FMN per subunit. Ref.6

Subunit structure

Homodimer. Ref.6

Sequence similarities

Belongs to the nitroreductase family.

Ontologies

Keywords
   LigandFMN
Flavoprotein
NAD
NADP
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Molecular functionFMN binding

Inferred from direct assay Ref.6. Source: EcoCyc

oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Putative NAD(P)H nitroreductase ydjA
PRO_0000169002

Regions

Nucleotide binding10 – 123FMN
Nucleotide binding121 – 1266NAD or NADP Potential
Nucleotide binding131 – 1333FMN

Sites

Binding site351FMN; shared with dimeric partner
Binding site391FMN; shared with dimeric partner

Secondary structure

............................... 183
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ACY1 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: A69809EE378847CB

FASTA18320,059
        10         20         30         40         50         60 
MDALELLINR RSASRLAEPA PTGEQLQNIL RAGMRAPDHK SMQPWHFFVI EGEGRERFSA 

        70         80         90        100        110        120 
VLEQGAIAAG SDDKAIDKAR NAPFRAPLII TVVAKCEENH KVPRWEQEMS AGCAVMAMQM 

       130        140        150        160        170        180 
AAVAQGFGGI WRSGALTESP VVREAFGCRE QDKIVGFLYL GTPQLKASTS INVPDPTPFV 


TYF 

« Hide

References

« Hide 'large scale' references
[1]"Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme."
Sawers G., Heider J., Zehelein E., Boeck A.
J. Bacteriol. 173:4983-4993(1991) [PubMed: 1650339] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: B / BL21.
[6]"Crystal structure of a minimal nitroreductase, ydjA, from Escherichia coli K12 with and without FMN cofactor."
Choi J.-W., Lee J., Nishi K., Kim Y.-S., Jung C.-H., Kim J.-S.
J. Mol. Biol. 377:258-267(2008) [PubMed: 18241886] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN, COFACTOR, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M68961 Genomic DNA. Translation: AAA24245.1.
U00096 Genomic DNA. Translation: AAC74835.1.
AP009048 Genomic DNA. Translation: BAA15556.1.
PIRA40360.
RefSeqNP_416279.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BM1X-ray2.00A/B1-183[»]
3BM2X-ray2.10A/B1-183[»]
ProteinModelPortalP0ACY1.
SMRP0ACY1. Positions 1-183.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-31841N.
IntActP0ACY1. 11 interactions.
MINTMINT-1239401.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000000798; EBESCP00000000798; EBESCG00000000667.
EBESCT00000017722; EBESCP00000017013; EBESCG00000016778.
GeneID945964.
GenomeReviewsGene locus JW1754 in contig AP009048_GR.
Gene locus b1765 in contig U00096_GR.
KEGGecj:JW1754.
eco:b1765.
PATRIC32118841. VBIEscCol129921_1838.

Organism-specific databases

EchoBASEEB1124.
EcoGeneEG11134. ydjA.

Phylogenomic databases

eggNOGCOG0778.
GeneTreeEBGT00050000010740.
HOGENOMHBG751302.
OMALNRVSVP.
PhylomeDBP0ACY1.
ProtClustDBPRK10828.

Enzyme and pathway databases

BioCycEcoCyc:EG11134-MONOMER.

Gene expression databases

GenevestigatorP0ACY1.

Family and domain databases

InterProIPR000415. Nitroreductase-like.
[Graphical view]
Gene3DG3DSA:3.40.109.10. G3DSA:3.40.109.10. 1 hit.
PfamPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMSSF55469. Nitroreductase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameYDJA_ECOLI
AccessionPrimary (citable) accession number: P0ACY1
Secondary accession number(s): P24250
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families