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Protein

HTH-type transcriptional repressor FabR

Gene

fabR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds the promoter region of at least fabA and fabB, but probably not yqfA (PubMed:11160901, PubMed:19854834, PubMed:21276098). Represses the transcription of fabA and fabB, involved in unsaturated fatty acid (UFA) biosynthesis (PubMed:11859088). By controlling UFA production, FabR directly influences the physical properties of the membrane bilayer.4 Publications

Enzyme regulationi

Has been suggested to require either an unsaturated acyl carrier protein or unsaturated acyl-CoA (but not their saturated equivalents) for DNA-binding (PubMed:19854834). Another group suggests that unsaturated thioesters are not essential but act instead to enhance DNA-binding (PubMed:21276098).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi52 – 71H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11394-MONOMER.
ECOL316407:JW3935-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional repressor FabR1 Publication
Gene namesi
Name:fabR1 Publication
Synonyms:yijC
Ordered Locus Names:b3963, JW3935
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11394. fabR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Significantly increased levels of unsaturated fatty acids (UFA) (PubMed:11859088, PubMed:19854834). Double fadR-fabR deletions have increased levels of saturated fatty acids, suggesting a functional fadR gene is required for fabR function (PubMed:11859088). Increased transcription of fabA and fabB (PubMed:11859088, PubMed:21276098).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000706411 – 234HTH-type transcriptional repressor FabRAdd BLAST234

Proteomic databases

PaxDbiP0ACU5.
PRIDEiP0ACU5.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi4261883. 155 interactors.
DIPiDIP-12520N.
STRINGi511145.b3963.

Structurei

3D structure databases

ProteinModelPortaliP0ACU5.
SMRiP0ACU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 89HTH tetR-typePROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105EJ2. Bacteria.
COG1309. LUCA.
HOGENOMiHOG000270364.
InParanoidiP0ACU5.
OMAiAYWYRKE.
PhylomeDBiP0ACU5.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01190. HTH_type_FabR. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR023764. Tscrpt_reg_HTH_FabR.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFILWYSASS TFGKDSDIVM GVRAQQKEKT RRSLVEAAFS QLSAERSFAS
60 70 80 90 100
LSLREVAREA GIAPTSFYRH FRDVDELGLT MVDESGLMLR QLMRQARQRI
110 120 130 140 150
AKGGSVIRTS VSTFMEFIGN NPNAFRLLLR ERSGTSAAFR AAVAREIQHF
160 170 180 190 200
IAELADYLEL ENHMPRAFTE AQAEAMVTIV FSAGAEALDV GVEQRRQLEE
210 220 230
RLVLQLRMIS KGAYYWYRRE QEKTAIIPGN VKDE
Length:234
Mass (Da):26,553
Last modified:November 2, 2016 - v2
Checksum:i326336C26008274E
GO

Sequence cautioni

The sequence AAC76945 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47S → T in CAA46823 (PubMed:1447162).Curated1
Sequence conflicti61G → V in CAA46823 (PubMed:1447162).Curated1
Sequence conflicti88 – 89ML → IV in CAA46823 (PubMed:1447162).Curated2
Sequence conflicti93 – 94MR → IG in CAA46823 (PubMed:1447162).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti61G → V in strain K12 / BW25113, may not bind DNA. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66026 Genomic DNA. Translation: CAA46823.1.
U00006 Genomic DNA. Translation: AAC43069.1.
U00096 Genomic DNA. Translation: AAC76945.2. Different initiation.
AP009048 Genomic DNA. Translation: BAE77348.1.
RefSeqiNP_418398.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76945; AAC76945; b3963.
BAE77348; BAE77348; BAE77348.
GeneIDi948460.
KEGGiecj:JW3935.
eco:b3963.
PATRICi32123447. VBIEscCol129921_4084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66026 Genomic DNA. Translation: CAA46823.1.
U00006 Genomic DNA. Translation: AAC43069.1.
U00096 Genomic DNA. Translation: AAC76945.2. Different initiation.
AP009048 Genomic DNA. Translation: BAE77348.1.
RefSeqiNP_418398.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP0ACU5.
SMRiP0ACU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261883. 155 interactors.
DIPiDIP-12520N.
STRINGi511145.b3963.

Proteomic databases

PaxDbiP0ACU5.
PRIDEiP0ACU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76945; AAC76945; b3963.
BAE77348; BAE77348; BAE77348.
GeneIDi948460.
KEGGiecj:JW3935.
eco:b3963.
PATRICi32123447. VBIEscCol129921_4084.

Organism-specific databases

EchoBASEiEB1367.
EcoGeneiEG11394. fabR.

Phylogenomic databases

eggNOGiENOG4105EJ2. Bacteria.
COG1309. LUCA.
HOGENOMiHOG000270364.
InParanoidiP0ACU5.
OMAiAYWYRKE.
PhylomeDBiP0ACU5.

Enzyme and pathway databases

BioCyciEcoCyc:EG11394-MONOMER.
ECOL316407:JW3935-MONOMER.

Miscellaneous databases

PROiP0ACU5.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01190. HTH_type_FabR. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR023764. Tscrpt_reg_HTH_FabR.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABR_ECOLI
AccessioniPrimary (citable) accession number: P0ACU5
Secondary accession number(s): P27307, Q2M8Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 2, 2016
Last modified: November 30, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Probably part of the fabR-yijD operon.1 Publication

Caution

It is uncertain whether Met-1 or Met-20 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.