P0ACU5 (FABR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional repressor FabR

Gene names

Name:	fabR
Synonyms:	yijC
Ordered Locus Names:	b3963, JW3935

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses the transcription of fabB, involved in unsaturated fatty acid (UFA) biosynthesis. By controlling UFA production, FabR directly influences the physical properties of the membrane bilayer. Ref.5 Ref.6
Subunit structure	Homodimer Probable.
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_01190.
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.
Sequence caution	The sequence AAC43069.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE77348.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA46823.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP negative regulation of fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.5Ref.6. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.5Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	HTH-type transcriptional repressor FabR HAMAP-Rule MF_01190		PRO_0000070641
Regions
	Domain	10 – 70	61	HTH tetR-type
	DNA binding	33 – 52	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	28	1	S → T in CAA46823. Ref.1
	Sequence conflict	42	1	G → V in CAA46823. Ref.1
	Sequence conflict	69 – 70	2	ML → IV in CAA46823. Ref.1
	Sequence conflict	74 – 75	2	MR → IG in CAA46823. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0ACU5 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: ACA9739EA947E256	FASTA	215	24,404
	10 20 30 40 50 60 MGVRAQQKEK TRRSLVEAAF SQLSAERSFA SLSLREVARE AGIAPTSFYR HFRDVDELGL 70 80 90 100 110 120 TMVDESGLML RQLMRQARQR IAKGGSVIRT SVSTFMEFIG NNPNAFRLLL RERSGTSAAF 130 140 150 160 170 180 RAAVAREIQH FIAELADYLE LENHMPRAFT EAQAEAMVTI VFSAGAEALD VGVEQRRQLE 190 200 210 ERLVLQLRMI SKGAYYWYRR EQEKTAIIPG NVKDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Physical map of the oxyR-trmA region (minute 89.3) of the Escherichia coli chromosome." Gustafsson C., Warne S.R. J. Bacteriol. 174:7878-7879(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Phylogenetic footprinting of transcription factor binding sites in proteobacterial genomes." McCue L.A., Thompson W., Carmack C.S., Ryan M.P., Liu J.S., Derbyshire V., Lawrence C.E. Nucleic Acids Res. 29:774-782(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ROLE IN REGULATION OF FATTY ACID BIOSYNTHESIS.
[6]	"The FabR (YijC) transcription factor regulates unsaturated fatty acid biosynthesis in Escherichia coli." Zhang Y.-M., Marrakchi H., Rock C.O. J. Biol. Chem. 277:15558-15565(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66026 Genomic DNA. Translation: CAA46823.1. Different initiation. U00006 Genomic DNA. Translation: AAC43069.1. Different initiation. U00096 Genomic DNA. Translation: AAC76945.2. AP009048 Genomic DNA. Translation: BAE77348.1. Different initiation.
RefSeq	NP_418398.2. NC_000913.2. YP_491489.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACU5.
SMR	P0ACU5. Positions 4-198.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3963.
Proteomic databases
PaxDb	P0ACU5.
PRIDE	P0ACU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76945; AAC76945; b3963. BAE77348; BAE77348; BAE77348.
GeneID	12932889. 948460.
KEGG	ecj:Y75_p3225. eco:b3963.
PATRIC	32123447. VBIEscCol129921_4084.
Organism-specific databases
EchoBASE	EB1367.
EcoGene	EG11394. fabR.
Phylogenomic databases
eggNOG	COG1309.
HOGENOM	HOG000270364.
OMA	AYWYRKE.
ProtClustDB	PRK11202.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11394-MONOMER. ECOL316407:JW3935-MONOMER.
Gene expression databases
Genevestigator	P0ACU5.
Family and domain databases
Gene3D	1.10.357.10. 1 hit.
HAMAP	MF_01190. HTH-type_FabR.
InterPro	IPR009057. Homeodomain-like. IPR001647. HTH_TetR. IPR015893. Tet_transcr_reg_TetR-like_C. IPR023764. Tscrpt_reg_HTH_FabR. [Graphical view]
Pfam	PF00440. TetR_N. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS01081. HTH_TETR_1. False negative. PS50977. HTH_TETR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABR_ECOLI

Accession

Primary (citable) accession number: P0ACU5
Secondary accession number(s): P27307, Q2M8Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program