ID   RPIR_ECOLI              Reviewed;         296 AA.
AC   P0ACS7; P39266; P76791; Q2M6L4;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 124.
DE   RecName: Full=HTH-type transcriptional regulator RpiR;
DE   AltName: Full=Als operon repressor;
GN   Name=rpiR; Synonyms=alsR, yjcY; OrderedLocusNames=b4089, JW4050;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8576032; DOI=10.1128/jb.178.4.1003-1011.1996;
RA   Soerensen K.I., Hove-Jensen B.;
RT   "Ribose catabolism of Escherichia coli: characterization of the rpiB gene
RT   encoding ribose phosphate isomerase B and of the rpiR gene, which is
RT   involved in regulation of rpiB expression.";
RL   J. Bacteriol. 178:1003-1011(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION IN ALS REPRESSION.
RX   PubMed=9401019; DOI=10.1128/jb.179.24.7631-7637.1997;
RA   Kim C., Song S., Park C.;
RT   "The D-allose operon of Escherichia coli K-12.";
RL   J. Bacteriol. 179:7631-7637(1997).
CC   -!- FUNCTION: Regulatory protein involved in rpiB gene repression. Also
CC       involved in als operon repression. {ECO:0000269|PubMed:9401019}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA96988.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; X82203; CAA57687.1; -; Genomic_DNA.
DR   EMBL; U14003; AAA96988.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC77050.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78092.1; -; Genomic_DNA.
DR   PIR; S56317; S56317.
DR   RefSeq; NP_418513.4; NC_000913.3.
DR   RefSeq; WP_000083216.1; NZ_STEB01000014.1.
DR   AlphaFoldDB; P0ACS7; -.
DR   SMR; P0ACS7; -.
DR   BioGRID; 4262682; 173.
DR   IntAct; P0ACS7; 5.
DR   STRING; 511145.b4089; -.
DR   jPOST; P0ACS7; -.
DR   PaxDb; 511145-b4089; -.
DR   DNASU; 948603; -.
DR   EnsemblBacteria; AAC77050; AAC77050; b4089.
DR   GeneID; 948603; -.
DR   KEGG; ecj:JW4050; -.
DR   KEGG; eco:b4089; -.
DR   PATRIC; fig|511145.12.peg.4216; -.
DR   EchoBASE; EB2353; -.
DR   eggNOG; COG1737; Bacteria.
DR   HOGENOM; CLU_055769_0_5_6; -.
DR   InParanoid; P0ACS7; -.
DR   OMA; EDMILWN; -.
DR   OrthoDB; 8582409at2; -.
DR   PhylomeDB; P0ACS7; -.
DR   BioCyc; EcoCyc:G7821-MONOMER; -.
DR   PRO; PR:P0ACS7; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:1901135; P:carbohydrate derivative metabolic process; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:EcoCyc.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   CDD; cd05013; SIS_RpiR; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR000281; HTH_RpiR.
DR   InterPro; IPR047640; RpiR-like.
DR   InterPro; IPR035472; RpiR-like_SIS.
DR   InterPro; IPR001347; SIS_dom.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR30514; GLUCOKINASE; 1.
DR   PANTHER; PTHR30514:SF1; HTH-TYPE TRANSCRIPTIONAL REGULATOR HEXR-RELATED; 1.
DR   Pfam; PF01418; HTH_6; 1.
DR   Pfam; PF01380; SIS; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF53697; SIS domain; 1.
DR   PROSITE; PS51071; HTH_RPIR; 1.
DR   PROSITE; PS51464; SIS; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..296
FT                   /note="HTH-type transcriptional regulator RpiR"
FT                   /id="PRO_0000068620"
FT   DOMAIN          14..90
FT                   /note="HTH rpiR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00390"
FT   DOMAIN          134..274
FT                   /note="SIS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00797"
FT   DNA_BIND        50..69
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00390"
SQ   SEQUENCE   296 AA;  32362 MW;  7946848F0798E200 CRC64;
     MSQSEFDSAL PNGIGLAPYL RMKQEGMTEN ESRIVEWLLK PGNLSCAPAI KDVAEALAVS
     EAMIVKVSKL LGFSGFRNLR SALEDYFSQS EQVLPSELAF DEAPQDVVNK VFNITLRTIM
     EGQSIVNVDE IHRAARFFYQ ARQRDLYGAG GSNAICADVQ HKFLRIGVRC QAYPDAHIMM
     MSASLLQEGD VVLVVTHSGR TSDVKAAVEL AKKNGAKIIC ITHSYHSPIA KLADYIICSP
     APETPLLGRN ASARILQLTL LDAFFVSVAQ LNIEQANINM QKTGAIVDFF SPGALK
//