Reviewed, UniProtKB/Swiss-Prot P0ACS7 (RPIR_ECOLI)

Names and origin

Protein names

Recommended name:
    HTH-type transcriptional regulator rpiR
Alternative name(s):
    Als operon repressor

Gene names

Name:	rpiR
Synonyms:	alsR, yjcY
Ordered Locus Names:	b4089, JW4050

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulatory protein involved in rpiB gene repression. Also involved in als operon repression. Ref.5
Sequence similarities	Contains 1 HTH rpiR-type DNA-binding domain. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sugar binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	HTH-type transcriptional regulator rpiR		PRO_0000068620
Regions
	Domain	14 – 90	77	HTH rpiR-type
	Domain	135 – 296	162	SIS
	DNA binding	50 – 69	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ACS7-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 7946848F0798E200	FASTA	296	32,362
	10 20 30 40 50 60 MSQSEFDSAL PNGIGLAPYL RMKQEGMTEN ESRIVEWLLK PGNLSCAPAI KDVAEALAVS 70 80 90 100 110 120 EAMIVKVSKL LGFSGFRNLR SALEDYFSQS EQVLPSELAF DEAPQDVVNK VFNITLRTIM 130 140 150 160 170 180 EGQSIVNVDE IHRAARFFYQ ARQRDLYGAG GSNAICADVQ HKFLRIGVRC QAYPDAHIMM 190 200 210 220 230 240 MSASLLQEGD VVLVVTHSGR TSDVKAAVEL AKKNGAKIIC ITHSYHSPIA KLADYIICSP 250 260 270 280 290 APETPLLGRN ASARILQLTL LDAFFVSVAQ LNIEQANINM QKTGAIVDFF SPGALK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression." Soerensen K.I., Hove-Jensen B. J. Bacteriol. 178:1003-1011(1996) [PubMed: 8576032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The D-allose operon of Escherichia coli K-12." Kim C., Song S., Park C. J. Bacteriol. 179:7631-7637(1997) [PubMed: 9401019] [Abstract] Cited for: FUNCTION IN ALS REPRESSION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82203 Genomic DNA. Translation: CAA57687.1. U14003 Genomic DNA. Translation: AAA96988.1. Different initiation. U00096 Genomic DNA. Translation: AAC77050.1. Different initiation. AP009048 Genomic DNA. Translation: BAE78092.1.
PIR	S56317.
RefSeq	AP_004591.1. NP_418513.4.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	948603.
GenomeReviews	Gene locus JW4050 in contig AP009048_GR. Gene locus b4089 in contig U00096_GR.
KEGG	ecj:JW4050. eco:b4089.
Organism-specific databases
EchoBASE	EB2353.
EcoGene	EG12459. rpiR.
CMR	Search...
Phylogenomic databases
HOGENOM	P0ACS7.
OMA	P0ACS7. DSSERVY.
Enzyme and pathway databases
BioCyc	EcoCyc:G7821-MON.
Family and domain databases
InterPro	IPR000281. HTH_RpiR. IPR001347. SIS. [Graphical view]
Pfam	PF01418. HTH_6. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
PROSITE	PS51071. HTH_RPIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPIR_ECOLI

Accession

Primary (citable) accession number: P0ACS7
Secondary accession number(s): P39266, P76791, Q2M6L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)