P0ACS7 (RPIR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator RpiR

Alternative name(s):
Als operon repressor

Gene names

Name:	rpiR
Synonyms:	alsR, yjcY
Ordered Locus Names:	b4089, JW4050

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory protein involved in rpiB gene repression. Also involved in als operon repression. Ref.5
Sequence similarities	Contains 1 HTH rpiR-type DNA-binding domain. Contains 1 SIS domain.
Sequence caution	The sequence AAA96988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.5. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	HTH-type transcriptional regulator RpiR		PRO_0000068620
Regions
	Domain	14 – 90	77	HTH rpiR-type
	Domain	134 – 274	141	SIS
	DNA binding	50 – 69	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ACS7 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 7946848F0798E200	FASTA	296	32,362
	10 20 30 40 50 60 MSQSEFDSAL PNGIGLAPYL RMKQEGMTEN ESRIVEWLLK PGNLSCAPAI KDVAEALAVS 70 80 90 100 110 120 EAMIVKVSKL LGFSGFRNLR SALEDYFSQS EQVLPSELAF DEAPQDVVNK VFNITLRTIM 130 140 150 160 170 180 EGQSIVNVDE IHRAARFFYQ ARQRDLYGAG GSNAICADVQ HKFLRIGVRC QAYPDAHIMM 190 200 210 220 230 240 MSASLLQEGD VVLVVTHSGR TSDVKAAVEL AKKNGAKIIC ITHSYHSPIA KLADYIICSP 250 260 270 280 290 APETPLLGRN ASARILQLTL LDAFFVSVAQ LNIEQANINM QKTGAIVDFF SPGALK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression." Soerensen K.I., Hove-Jensen B. J. Bacteriol. 178:1003-1011(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The D-allose operon of Escherichia coli K-12." Kim C., Song S., Park C. J. Bacteriol. 179:7631-7637(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ALS REPRESSION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82203 Genomic DNA. Translation: CAA57687.1. U14003 Genomic DNA. Translation: AAA96988.1. Different initiation. U00096 Genomic DNA. Translation: AAC77050.2. AP009048 Genomic DNA. Translation: BAE78092.1.
PIR	S56317.
RefSeq	NP_418513.4. NC_000913.2. YP_492233.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACS7.
SMR	P0ACS7. Positions 122-283.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ACS7. 5 interactions.
STRING	511145.b4089.
Proteomic databases
PRIDE	P0ACS7.
Protocols and materials databases
DNASU	948603.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77050; AAC77050; b4089. BAE78092; BAE78092; BAE78092.
GeneID	12933688. 948603.
KEGG	ecj:Y75_p3977. eco:b4089.
PATRIC	32123735. VBIEscCol129921_4216.
Organism-specific databases
EchoBASE	EB2353.
EcoGene	EG12459. rpiR.
Phylogenomic databases
eggNOG	COG1737.
HOGENOM	HOG000027157.
OMA	KNASARI.
ProtClustDB	PRK11337.
Enzyme and pathway databases
BioCyc	EcoCyc:G7821-MONOMER. ECOL316407:JW4050-MONOMER.
Gene expression databases
Genevestigator	P0ACS7.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000281. HTH_RpiR. IPR001347. SIS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01418. HTH_6. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
PROSITE	PS51071. HTH_RPIR. 1 hit. PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPIR_ECOLI

Accession

Primary (citable) accession number: P0ACS7
Secondary accession number(s): P39266, P76791, Q2M6L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program