Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Redox-sensitive transcriptional activator SoxR

Gene

soxR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates the transcription of the soxS gene which itself controls the superoxide response regulon. SoxR contains a 2Fe-2S iron-sulfur cluster that may act as a redox sensor system that recognizes superoxide. The variable redox state of the Fe-S cluster is employed in vivo to modulate the transcriptional activity of SoxR in response to specific types of oxidative stress. Upon reduction of 2Fe-2S cluster, SoxR reversibly loses its transcriptional activity, but retains its DNA binding affinity.

Redox potential

E0 is -285 mV.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119Iron-sulfur (2Fe-2S)1
Metal bindingi122Iron-sulfur (2Fe-2S)1
Metal bindingi124Iron-sulfur (2Fe-2S)1
Metal bindingi130Iron-sulfur (2Fe-2S)1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi14 – 33H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to oxidative stress Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD04132

Names & Taxonomyi

Protein namesi
Recommended name:
Redox-sensitive transcriptional activator SoxR
Gene namesi
Name:soxR
Synonyms:marC
Ordered Locus Names:b4063, JW4024
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10957 soxR

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981501 – 154Redox-sensitive transcriptional activator SoxRAdd BLAST154

Proteomic databases

PaxDbiP0ACS2
PRIDEiP0ACS2

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259601, 10 interactors
DIPiDIP-29848N
IntActiP0ACS2, 7 interactors
STRINGi316385.ECDH10B_4252

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 21Combined sources8
Helixi25 – 33Combined sources9
Turni43 – 45Combined sources3
Beta strandi47 – 49Combined sources3
Helixi53 – 66Combined sources14
Helixi70 – 77Combined sources8
Helixi88 – 119Combined sources32
Turni120 – 122Combined sources3
Helixi127 – 129Combined sources3
Turni131 – 133Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZHGX-ray2.80A1-154[»]
2ZHHX-ray3.20A1-154[»]
ProteinModelPortaliP0ACS2
SMRiP0ACS2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACS2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 79HTH merR-typePROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 130Might be part of a sensor regionAdd BLAST12

Phylogenomic databases

eggNOGiENOG4108Z4X Bacteria
COG0789 LUCA
HOGENOMiHOG000266085
InParanoidiP0ACS2
KOiK13639
OMAiNGNQRRY
PhylomeDBiP0ACS2

Family and domain databases

CDDicd01110 HTH_SoxR, 1 hit
InterProiView protein in InterPro
IPR009061 DNA-bd_dom_put_sf
IPR000551 MerR-type_HTH_dom
IPR010211 Redox-sen_tscrpt-act_SoxR
IPR015358 Tscrpt_reg_MerR_DNA-bd
PANTHERiPTHR30204:SF0 PTHR30204:SF0, 1 hit
PfamiView protein in Pfam
PF00376 MerR, 1 hit
PF09278 MerR-DNA-bind, 1 hit
PRINTSiPR00040 HTHMERR
SMARTiView protein in SMART
SM00422 HTH_MERR, 1 hit
SUPFAMiSSF46955 SSF46955, 1 hit
TIGRFAMsiTIGR01950 SoxR, 1 hit
PROSITEiView protein in PROSITE
PS00552 HTH_MERR_1, 1 hit
PS50937 HTH_MERR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKLPRIKA LLTPGEVAKR SGVAVSALHF YESKGLITSI RNSGNQRRYK
60 70 80 90 100
RDVLRYVAII KIAQRIGIPL ATIGEAFGVL PEGHTLSAKE WKQLSSQWRE
110 120 130 140 150
ELDRRIHTLV ALRDELDGCI GCGCLSRSDC PLRNPGDRLG EEGTGARLLE

DEQN
Length:154
Mass (Da):17,150
Last modified:November 22, 2005 - v1
Checksum:i9A715120860EEC9B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti20R → C in soxR102; constitutively activated allele. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60111 Genomic DNA Translation: AAA24641.1
X59593 Genomic DNA Translation: CAA42162.1
U00006 Genomic DNA Translation: AAC43157.1
U00096 Genomic DNA Translation: AAC77033.1
AP009048 Genomic DNA Translation: BAE78065.1
S72675 Genomic DNA Translation: AAB31680.1
PIRiJS0577
RefSeqiNP_418487.1, NC_000913.3
WP_000412428.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77033; AAC77033; b4063
BAE78065; BAE78065; BAE78065
GeneIDi948566
KEGGiecj:JW4024
eco:b4063
PATRICifig|1411691.4.peg.2642

Similar proteinsi

Entry informationi

Entry nameiSOXR_ECOLI
AccessioniPrimary (citable) accession number: P0ACS2
Secondary accession number(s): P22538, Q2M6P1, Q53442
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health