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Protein

Redox-sensitive transcriptional activator SoxR

Gene

soxR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates the transcription of the soxS gene which itself controls the superoxide response regulon. SoxR contains a 2Fe-2S iron-sulfur cluster that may act as a redox sensor system that recognizes superoxide. The variable redox state of the Fe-S cluster is employed in vivo to modulate the transcriptional activity of SoxR in response to specific types of oxidative stress. Upon reduction of 2Fe-2S cluster, SoxR reversibly loses its transcriptional activity, but retains its DNA binding affinity.

Redox potential

E0 is -285 mV.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119Iron-sulfur (2Fe-2S)1
Metal bindingi122Iron-sulfur (2Fe-2S)1
Metal bindingi124Iron-sulfur (2Fe-2S)1
Metal bindingi130Iron-sulfur (2Fe-2S)1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi14 – 33H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to oxidative stress Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

2Fe-2S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD04132.
ECOL316407:JW4024-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Redox-sensitive transcriptional activator SoxR
Gene namesi
Name:soxR
Synonyms:marC
Ordered Locus Names:b4063, JW4024
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10957. soxR.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981501 – 154Redox-sensitive transcriptional activator SoxRAdd BLAST154

Proteomic databases

PaxDbiP0ACS2.
PRIDEiP0ACS2.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259601. 9 interactors.
DIPiDIP-29848N.
IntActiP0ACS2. 7 interactors.
STRINGi511145.b4063.

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 21Combined sources8
Helixi25 – 33Combined sources9
Turni43 – 45Combined sources3
Beta strandi47 – 49Combined sources3
Helixi53 – 66Combined sources14
Helixi70 – 77Combined sources8
Helixi88 – 119Combined sources32
Turni120 – 122Combined sources3
Helixi127 – 129Combined sources3
Turni131 – 133Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZHGX-ray2.80A1-154[»]
2ZHHX-ray3.20A1-154[»]
ProteinModelPortaliP0ACS2.
SMRiP0ACS2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACS2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 79HTH merR-typePROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 130Might be part of a sensor regionAdd BLAST12

Sequence similaritiesi

Contains 1 HTH merR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108Z4X. Bacteria.
COG0789. LUCA.
HOGENOMiHOG000266085.
InParanoidiP0ACS2.
KOiK13639.
OMAiNGNQRRY.
PhylomeDBiP0ACS2.

Family and domain databases

CDDicd01110. HTH_SoxR. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR010211. Redox-sen_tscrpt-act_SoxR.
IPR015358. Tscrpt_reg_MerR_DNA-bd.
[Graphical view]
PANTHERiPTHR30204:SF0. PTHR30204:SF0. 1 hit.
PfamiPF00376. MerR. 1 hit.
PF09278. MerR-DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00040. HTHMERR.
SMARTiSM00422. HTH_MERR. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR01950. SoxR. 1 hit.
PROSITEiPS00552. HTH_MERR_1. 1 hit.
PS50937. HTH_MERR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKLPRIKA LLTPGEVAKR SGVAVSALHF YESKGLITSI RNSGNQRRYK
60 70 80 90 100
RDVLRYVAII KIAQRIGIPL ATIGEAFGVL PEGHTLSAKE WKQLSSQWRE
110 120 130 140 150
ELDRRIHTLV ALRDELDGCI GCGCLSRSDC PLRNPGDRLG EEGTGARLLE

DEQN
Length:154
Mass (Da):17,150
Last modified:November 22, 2005 - v1
Checksum:i9A715120860EEC9B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti20R → C in soxR102; constitutively activated allele. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60111 Genomic DNA. Translation: AAA24641.1.
X59593 Genomic DNA. Translation: CAA42162.1.
U00006 Genomic DNA. Translation: AAC43157.1.
U00096 Genomic DNA. Translation: AAC77033.1.
AP009048 Genomic DNA. Translation: BAE78065.1.
S72675 Genomic DNA. Translation: AAB31680.1.
PIRiJS0577.
RefSeqiNP_418487.1. NC_000913.3.
WP_000412428.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77033; AAC77033; b4063.
BAE78065; BAE78065; BAE78065.
GeneIDi948566.
KEGGiecj:JW4024.
eco:b4063.
PATRICi32123671. VBIEscCol129921_4184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60111 Genomic DNA. Translation: AAA24641.1.
X59593 Genomic DNA. Translation: CAA42162.1.
U00006 Genomic DNA. Translation: AAC43157.1.
U00096 Genomic DNA. Translation: AAC77033.1.
AP009048 Genomic DNA. Translation: BAE78065.1.
S72675 Genomic DNA. Translation: AAB31680.1.
PIRiJS0577.
RefSeqiNP_418487.1. NC_000913.3.
WP_000412428.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZHGX-ray2.80A1-154[»]
2ZHHX-ray3.20A1-154[»]
ProteinModelPortaliP0ACS2.
SMRiP0ACS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259601. 9 interactors.
DIPiDIP-29848N.
IntActiP0ACS2. 7 interactors.
STRINGi511145.b4063.

Proteomic databases

PaxDbiP0ACS2.
PRIDEiP0ACS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77033; AAC77033; b4063.
BAE78065; BAE78065; BAE78065.
GeneIDi948566.
KEGGiecj:JW4024.
eco:b4063.
PATRICi32123671. VBIEscCol129921_4184.

Organism-specific databases

EchoBASEiEB0950.
EcoGeneiEG10957. soxR.

Phylogenomic databases

eggNOGiENOG4108Z4X. Bacteria.
COG0789. LUCA.
HOGENOMiHOG000266085.
InParanoidiP0ACS2.
KOiK13639.
OMAiNGNQRRY.
PhylomeDBiP0ACS2.

Enzyme and pathway databases

BioCyciEcoCyc:PD04132.
ECOL316407:JW4024-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACS2.
PROiP0ACS2.

Family and domain databases

CDDicd01110. HTH_SoxR. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR010211. Redox-sen_tscrpt-act_SoxR.
IPR015358. Tscrpt_reg_MerR_DNA-bd.
[Graphical view]
PANTHERiPTHR30204:SF0. PTHR30204:SF0. 1 hit.
PfamiPF00376. MerR. 1 hit.
PF09278. MerR-DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00040. HTHMERR.
SMARTiSM00422. HTH_MERR. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR01950. SoxR. 1 hit.
PROSITEiPS00552. HTH_MERR_1. 1 hit.
PS50937. HTH_MERR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSOXR_ECOLI
AccessioniPrimary (citable) accession number: P0ACS2
Secondary accession number(s): P22538, Q2M6P1, Q53442
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.