P0ACR9 (MPRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor mprA

Alternative name(s):
Protein emrR

Gene names

Name:	mprA
Synonyms:	emrR
Ordered Locus Names:	b2684, JW2659

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Negative regulator of the multidrug operon emrAB. Ref.6
Induction	Autoregulated.
Sequence similarities	Contains 1 HTH marR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Transcriptional repressor mprA		PRO_0000054373
Regions
	Domain	26 – 160	135	HTH marR-type

Sequences

	Sequence		Length	Mass (Da)
	P0ACR9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: BF4D3E34DAD0718A	FASTA	176	20,563
	10 20 30 40 50 60 MDSSFTPIEQ MLKFRASRHE DFPYQEILLT RLCMHMQSKL LENRNKMLKA QGINETLFMA 70 80 90 100 110 120 LITLESQENH SIQPSELSCA LGSSRTNATR IADELEKRGW IERRESDNDR RCLHLQLTEK 130 140 150 160 170 GHEFLREVLP PQHNCLHQLW SALSTTEKDQ LEQITRKLLS RLDQMEQDGV VLEAMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants." del Castillo I., Gonzalez-Pastor J.E., San Millan J.L., Moreno F. J. Bacteriol. 173:3924-3929(1991) [PubMed: 1840583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Emr, an Escherichia coli locus for multidrug resistance." Lomovskaya O., Lewis K. Proc. Natl. Acad. Sci. U.S.A. 89:8938-8942(1992) [PubMed: 1409590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 152-176.
[6]	"EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB." Lomovskaya O., Lewis K., Matin A. J. Bacteriol. 177:2328-2334(1995) [PubMed: 7730261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5, FUNCTION. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54151 Genomic DNA. Translation: CAA38090.1. U00096 Genomic DNA. Translation: AAC75731.1. AP009048 Genomic DNA. Translation: BAA16546.1. M86657 Genomic DNA. No translation available. U19993 Genomic DNA. No translation available.
PIR	S14473.
RefSeq	NP_417169.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0ACR9.
SMR	P0ACR9. Positions 26-159.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ACR9. 11 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000573; EBESCP00000000573; EBESCG00000000479. EBESCT00000015890; EBESCP00000015181; EBESCG00000014950.
GeneID	945282.
GenomeReviews	Gene locus JW2659 in contig AP009048_GR. Gene locus b2684 in contig U00096_GR.
KEGG	ecj:JW2659. eco:b2684.
PATRIC	32120760. VBIEscCol129921_2777.
Organism-specific databases
EchoBASE	EB0598.
EcoGene	EG10603. mprA.
Phylogenomic databases
eggNOG	COG1846.
GeneTree	EBGT00050000010973.
HOGENOM	HBG752581.
OMA	ESHSIQP.
PhylomeDB	P0ACR9.
ProtClustDB	PRK10870.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10603-MONOMER.
Gene expression databases
Genevestigator	P0ACR9.
Family and domain databases
InterPro	IPR000835. Tscrpt_reg_HTH_MarR-typ. IPR023187. Tscrpt_reg_MarR-type_CS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K03712.
Pfam	PF01047. MarR. 1 hit. [Graphical view]
PRINTS	PR00598. HTHMARR.
SMART	SM00347. HTH_MARR. 1 hit. [Graphical view]
PROSITE	PS01117. HTH_MARR_1. 1 hit. PS50995. HTH_MARR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPRA_ECOLI

Accession

Primary (citable) accession number: P0ACR9
Secondary accession number(s): P24201, P77027

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program