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Protein

Hydrogen peroxide-inducible genes activator

Gene

oxyR

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrogen peroxide sensor. Activates the expression of a regulon of hydrogen peroxide-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA. OxyR expression is negatively autoregulated by binding to a 43 bp region upstream of its own coding sequence. OxyR is inactivated by reduction of its essential disulfide bond by the product of GrxA, itself positively regulated by OxyR. Has also a positive regulatory effect on the production of surface proteins that control the colony morphology and auto-aggregation ability (By similarity).By similarity

Miscellaneous

Oxidized OxyR can be reduced and inactivated by glutaredoxin 1, the product of grxA, whose expression is regulated by OxyR itself.By similarity

Enzyme regulationi

Activated by oxidation of Cys-199 resulting in the alternative formation of cystine, sulfenic acid, S-nitroso- or glutathione-bound cysteine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi18 – 37H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogen peroxide-inducible genes activator
Alternative name(s):
Morphology and auto-aggregation control protein
Gene namesi
Name:oxyR
Ordered Locus Names:Z5519, ECs4890
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001057291 – 305Hydrogen peroxide-inducible genes activatorAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi180 ↔ 259By similarity
Disulfide bondi199 ↔ 208AlternateBy similarity
Modified residuei199Cysteine sulfenic acid (-SOH); alternateBy similarity1
Modified residuei199S-glutathionyl cysteine; alternateBy similarity1
Modified residuei199S-nitrosocysteine; alternateBy similarity1

Post-translational modificationi

Oxidized on Cys-199; the Cys-SOH formed in response to oxidative signaling triggers a conformational change and the onset of transcriptional activity with a specific DNA-binding affinity. Cys-199-SOH rapidly reacts with Cys-208-SH to form a disulfide bond (By similarity).By similarity
S-nitrosylation in response to nitrosative signaling triggers a conformational change and the onset of transcriptional activity with a specific DNA-binding affinity.By similarity
Glutathionylation in response to redox signaling triggers the onset of transcriptional activity with a specific DNA-binding affinity.By similarity

Keywords - PTMi

Disulfide bond, Glutathionylation, Oxidation, S-nitrosylation

Proteomic databases

PRIDEiP0ACQ6.

Interactioni

Subunit structurei

Homodimer and homotetramer.By similarity

Protein-protein interaction databases

STRINGi155864.Z5519.

Structurei

3D structure databases

ProteinModelPortaliP0ACQ6.
SMRiP0ACQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Phylogenomic databases

eggNOGiENOG4105C5Q. Bacteria.
ENOG410XNR2. LUCA.
HOGENOMiHOG000233514.
KOiK04761.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS50931. HTH_LYSR. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ACQ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRDLEYLV ALAEHRHFRR AADSCHVSQP TLSGQIRKLE DELGVMLLER
60 70 80 90 100
TSRKVLFTQA GMLLVDQART VLREVKVLKE MASQQGETMS GPLHIGLIPT
110 120 130 140 150
VGPYLLPHII PMLHQTFPKL EMYLHEAQTH QLLAQLDSGK LDCVILALVK
160 170 180 190 200
ESEAFIEVPL FDEPMLLAIY EDHPWANREC VPMADLAGEK LLMLEDGHCL
210 220 230 240 250
RDQAMGFCFE AGADEDTHFR ATSLETLRNM VAAGSGITLL PALAVPPERK
260 270 280 290 300
RDGVVYLPCI KPEPRRTIGL VYRPGSPLRS RYEQLAEAIR ARMDGHFDKV

LKQAV
Length:305
Mass (Da):34,276
Last modified:November 22, 2005 - v1
Checksum:i714EF4169CED2EC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59163.1.
BA000007 Genomic DNA. Translation: BAB38313.1.
PIRiB91240.
G86087.
RefSeqiNP_312917.1. NC_002695.1.
WP_001025939.1. NZ_NOKN01000002.1.

Genome annotation databases

EnsemblBacteriaiAAG59163; AAG59163; Z5519.
BAB38313; BAB38313; BAB38313.
GeneIDi914984.
KEGGiece:Z5519.
ecs:ECs4890.
PATRICifig|386585.9.peg.5114.

Similar proteinsi

Entry informationi

Entry nameiOXYR_ECO57
AccessioniPrimary (citable) accession number: P0ACQ6
Secondary accession number(s): P11721, P22471
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 22, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome