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Protein

Catabolite repressor/activator

Gene

cra

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Global transcriptional regulator, which plays an important role in the regulation of carbon metabolism. Activates transcription of genes encoding biosynthetic and oxidative enzymes (involved in Krebs cycle, glyoxylate shunt and gluconeogenesis, such as ppsA and fbp). Represses genes involved in sugar catabolism, such as fruB, pfkA, pykF and adhE. Binds asymmetrically to the two half-sites of its operator.8 Publications

Enzyme regulationi

Is displaced from DNA by low concentrations of fructose-1-phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi3 – 22H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00521.
ECOL316407:JW0078-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catabolite repressor/activator
Alternative name(s):
Fructose repressor
Gene namesi
Name:cra
Synonyms:fruC, fruR, shl
Ordered Locus Names:b0080, JW0078
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10338. cra.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001079451 – 334Catabolite repressor/activatorAdd BLAST334

Proteomic databases

EPDiP0ACP1.
PaxDbiP0ACP1.
PRIDEiP0ACP1.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4259359. 7 interactors.
DIPiDIP-35838N.
IntActiP0ACP1. 11 interactors.
MINTiMINT-1252433.
STRINGi511145.b0080.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 10Combined sources8
Helixi14 – 22Combined sources9
Turni25 – 29Combined sources5
Helixi33 – 45Combined sources13
Beta strandi63 – 69Combined sources7
Helixi74 – 89Combined sources16
Beta strandi93 – 98Combined sources6
Helixi103 – 115Combined sources13
Beta strandi119 – 123Combined sources5
Helixi132 – 135Combined sources4
Turni136 – 139Combined sources4
Beta strandi140 – 142Combined sources3
Beta strandi144 – 149Combined sources6
Turni153 – 155Combined sources3
Beta strandi157 – 161Combined sources5
Helixi163 – 175Combined sources13
Beta strandi180 – 187Combined sources8
Helixi192 – 205Combined sources14
Beta strandi212 – 220Combined sources9
Helixi222 – 232Combined sources11
Turni233 – 235Combined sources3
Beta strandi240 – 246Combined sources7
Helixi247 – 261Combined sources15
Beta strandi269 – 274Combined sources6
Helixi277 – 281Combined sources5
Beta strandi283 – 290Combined sources8
Helixi293 – 309Combined sources17
Beta strandi310 – 312Combined sources3
Beta strandi317 – 321Combined sources5
Beta strandi324 – 329Combined sources6
Helixi330 – 333Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UXCNMR-A1-57[»]
1UXDNMR-A1-57[»]
2IKSX-ray1.85A/B44-334[»]
ProteinModelPortaliP0ACP1.
SMRiP0ACP1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58HTH lacI-typePROSITE-ProRule annotationAdd BLAST58

Sequence similaritiesi

Contains 1 HTH lacI-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107B7Y. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000278142.
InParanoidiP0ACP1.
KOiK03435.
OMAiWANDSFP.
PhylomeDBiP0ACP1.

Family and domain databases

CDDicd01392. HTH_LacI. 1 hit.
Gene3Di1.10.260.40. 1 hit.
InterProiIPR012781. Fruct_sucro_rep.
IPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR001761. Peripla_BP/Lac1_sug-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
PF00532. Peripla_BP_1. 1 hit.
[Graphical view]
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
TIGRFAMsiTIGR02417. fruct_sucro_rep. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDEIARLA GVSRTTASYV INGKAKQYRV SDKTVEKVMA VVREHNYHPN
60 70 80 90 100
AVAAGLRAGR TRSIGLVIPD LENTSYTRIA NYLERQARQR GYQLLIACSE
110 120 130 140 150
DQPDNEMRCI EHLLQRQVDA IIVSTSLPPE HPFYQRWAND PFPIVALDRA
160 170 180 190 200
LDREHFTSVV GADQDDAEML AEELRKFPAE TVLYLGALPE LSVSFLREQG
210 220 230 240 250
FRTAWKDDPR EVHFLYANSY EREAAAQLFE KWLETHPMPQ ALFTTSFALL
260 270 280 290 300
QGVMDVTLRR DGKLPSDLAI ATFGDNELLD FLQCPVLAVA QRHRDVAERV
310 320 330
LEIVLASLDE PRKPKPGLTR IKRNLYRRGV LSRS
Length:334
Mass (Da):37,999
Last modified:November 22, 2005 - v1
Checksum:i503AE935CC2EC0C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35034 Genomic DNA. Translation: AAA24629.1.
X55034 Genomic DNA. Translation: CAA38857.1.
X55457 Genomic DNA. Translation: CAA39104.1.
U00096 Genomic DNA. Translation: AAC73191.1.
AP009048 Genomic DNA. Translation: BAB96648.1.
PIRiJU0298.
RefSeqiNP_414622.1. NC_000913.3.
WP_000762401.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73191; AAC73191; b0080.
BAB96648; BAB96648; BAB96648.
GeneIDi944804.
KEGGiecj:JW0078.
eco:b0080.
PATRICi32115263. VBIEscCol129921_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35034 Genomic DNA. Translation: AAA24629.1.
X55034 Genomic DNA. Translation: CAA38857.1.
X55457 Genomic DNA. Translation: CAA39104.1.
U00096 Genomic DNA. Translation: AAC73191.1.
AP009048 Genomic DNA. Translation: BAB96648.1.
PIRiJU0298.
RefSeqiNP_414622.1. NC_000913.3.
WP_000762401.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UXCNMR-A1-57[»]
1UXDNMR-A1-57[»]
2IKSX-ray1.85A/B44-334[»]
ProteinModelPortaliP0ACP1.
SMRiP0ACP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259359. 7 interactors.
DIPiDIP-35838N.
IntActiP0ACP1. 11 interactors.
MINTiMINT-1252433.
STRINGi511145.b0080.

Proteomic databases

EPDiP0ACP1.
PaxDbiP0ACP1.
PRIDEiP0ACP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73191; AAC73191; b0080.
BAB96648; BAB96648; BAB96648.
GeneIDi944804.
KEGGiecj:JW0078.
eco:b0080.
PATRICi32115263. VBIEscCol129921_0083.

Organism-specific databases

EchoBASEiEB0334.
EcoGeneiEG10338. cra.

Phylogenomic databases

eggNOGiENOG4107B7Y. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000278142.
InParanoidiP0ACP1.
KOiK03435.
OMAiWANDSFP.
PhylomeDBiP0ACP1.

Enzyme and pathway databases

BioCyciEcoCyc:PD00521.
ECOL316407:JW0078-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACP1.
PROiP0ACP1.

Family and domain databases

CDDicd01392. HTH_LacI. 1 hit.
Gene3Di1.10.260.40. 1 hit.
InterProiIPR012781. Fruct_sucro_rep.
IPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR001761. Peripla_BP/Lac1_sug-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
PF00532. Peripla_BP_1. 1 hit.
[Graphical view]
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
TIGRFAMsiTIGR02417. fruct_sucro_rep. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRA_ECOLI
AccessioniPrimary (citable) accession number: P0ACP1
Secondary accession number(s): P21168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Activation of transcription is observed when the Cra operator is upstream of the RNA polymerase binding site. Inhibition of transcription is observed when the operator overlaps or is downstream of the RNA polymerase binding site (PubMed:8655535).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.