Reviewed,
UniProtKB/Swiss-Prot P0ACN4 (ALLR_ECOLI)
Last modified
June 16, 2009.
Version 34.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: HTH-type transcriptional repressor allR Alternative name(s): Negative regulator of allantoin and glyoxylate utilization operons | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 271 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Negative regulator of allantoin and glyoxylate utilization operons. Binds to the gcl promoter and to the allS-allA intergenic region. Binding to DNA is abolished by glyoxylate. Ref.1 Ref.5 Ref.6 |
| Subunit structure | Homotetramer and homodimer. Homotetramer in its active, DNA-bound form. Homodimer in its inactive form. Ref.6 |
| Sequence similarities | Contains 1 HTH iclR-type DNA-binding domain. Contains 1 iclR-ED (iclR effector binding) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Ligand | DNA-binding |
| Molecular function | Repressor |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 271 | 271 | HTH-type transcriptional repressor allR | PRO_0000201753 | ||||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||||
| Domain | 21 – 83 | 63 | HTH iclR-type | |||||||||||||||||||||||||||||||||
| Domain | 98 – 267 | 170 | IclR-ED | |||||||||||||||||||||||||||||||||
| DNA binding | 43 – 62 | 20 | H-T-H motif Potential | |||||||||||||||||||||||||||||||||
| Region | 154 – 156 | 3 | Glyoxylate binding | |||||||||||||||||||||||||||||||||
| Region | 234 – 236 | 3 | Glyoxylate binding | |||||||||||||||||||||||||||||||||
Sites | ||||||||||||||||||||||||||||||||||||
| Binding site | 207 | 1 | Glyoxylate | |||||||||||||||||||||||||||||||||
| Binding site | 217 | 1 | Glyoxylate | |||||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||||
| Mutagenesis | 135 – 142 | 8 | CKSMVRMC → APA: Loss of repressor activity. Loss of DNA binding. Reduces assembly into functionally active tetramers. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 141 | 1 | M → A: No effect on repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 142 | 1 | C → W: Strongly reduced repressor activity. Reduces DNA binding. Prevents assembly into functionally active tetramers. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 215 | 1 | L → A: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 217 | 1 | C → A or S: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 234 | 1 | S → A or N: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6 | |||||||||||||||||||||||||||||||||
| Mutagenesis | 236 | 1 | S → A or M: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6 | |||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||
| Helix | 97 – 113 | 17 | ||||||||||||||||||||||||||||||||||
| Beta strand | 115 – 123 | 9 | ||||||||||||||||||||||||||||||||||
| Beta strand | 126 – 133 | 8 | ||||||||||||||||||||||||||||||||||
| Beta strand | 138 – 141 | 4 | ||||||||||||||||||||||||||||||||||
| Beta strand | 148 – 150 | 3 | ||||||||||||||||||||||||||||||||||
| Turn | 151 – 153 | 3 | ||||||||||||||||||||||||||||||||||
| Helix | 155 – 161 | 7 | ||||||||||||||||||||||||||||||||||
| Helix | 166 – 176 | 11 | ||||||||||||||||||||||||||||||||||
| Helix | 189 – 202 | 14 | ||||||||||||||||||||||||||||||||||
| Beta strand | 205 – 212 | 8 | ||||||||||||||||||||||||||||||||||
| Beta strand | 215 – 223 | 9 | ||||||||||||||||||||||||||||||||||
| Beta strand | 229 – 238 | 10 | ||||||||||||||||||||||||||||||||||
| Turn | 239 – 241 | 3 | ||||||||||||||||||||||||||||||||||
| Helix | 244 – 246 | 3 | ||||||||||||||||||||||||||||||||||
| Helix | 247 – 265 | 19 | ||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." Cusa E., Obradors N., Baldoma L., Badia J., Aguilar J. J. Bacteriol. 181:7479-7484(1999) [PubMed: 10601204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: K12 / ECL1. |
| [2] | "Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Regulation of the Escherichia coli allantoin regulon: coordinated function of the repressor AllR and the activator AllS." Rintoul M.R., Cusa E., Baldoma L., Badia J., Reitzer L., Aguilar J. J. Mol. Biol. 324:599-610(2002) [PubMed: 12460564] [Abstract] Cited for: FUNCTION, DNA-BINDING. Strain: K12. |
| [6] | "Structural and biochemical study of effector molecule recognition by the E.coli glyoxylate and allantoin utilization regulatory protein AllR." Walker J.R., Altamentova S., Ezersky A., Lorca G., Skarina T., Kudritska M., Ball L.J., Bochkarev A., Savchenko A. J. Mol. Biol. 358:810-828(2006) [PubMed: 16546208] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 97-271 IN COMPLEX WITH GLYOXALATE, FUNCTION, SUBUNIT, MUTAGENESIS OF 135-CYS--CYS-142; MET-141; CYS-142; LEU-215; CYS-217; SER-234 AND SER-236. Strain: K12 / BW25113. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U89024 Genomic DNA. Translation: AAB93847.1. U82664 Genomic DNA. Translation: AAB40259.1. U00096 Genomic DNA. Translation: AAC73608.1. AP009048 Genomic DNA. Translation: BAE76284.1. | |||||||||||||
| PIR | A64782. | ||||||||||||
| RefSeq | AP_001154.1. NP_415039.1. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| GeneID | 945333. | ||||||||||||
| GenomeReviews | Gene locus JW0494 in contig AP009048_GR. Gene locus b0506 in contig U00096_GR. | ||||||||||||
| KEGG | ecj:JW0494. eco:b0506. | ||||||||||||
Organism-specific databases | |||||||||||||
| EchoBASE | EB3382. | ||||||||||||
| EcoGene | EG13616. allR. | ||||||||||||
| CMR | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P0ACN4. | ||||||||||||
| OMA | P0ACN4. AGPKERM. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | EcoCyc:G6276-MON. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR014757. Transcript_reg_IclR_C. IPR005471. Transcript_reg_IclR_N. [Graphical view] | ||||||||||||
| Pfam | PF09339. HTH_IclR. 1 hit. PF01614. IclR. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00346. HTH_ICLR. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS51077. HTH_ICLR. 1 hit. PS51078. ICLR_ED. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| BindingDB | P0ACN4. | ||||||||||||
Entry information
| Entry name | ALLR_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0ACN4 Secondary accession number(s): P77734, Q2MBS2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


