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Protein

HTH-type transcriptional repressor AllR

Gene

allR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of allantoin and glyoxylate utilization operons. Binds to the gcl promoter and to the allS-allA intergenic region. Binding to DNA is abolished by glyoxylate.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei207 – 2071Glyoxylate
Binding sitei217 – 2171Glyoxylate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi43 – 6220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6276-MONOMER.
ECOL316407:JW0494-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional repressor AllR
Alternative name(s):
Negative regulator of allantoin and glyoxylate utilization operons
Gene namesi
Name:allR
Synonyms:glxA3, ybbU
Ordered Locus Names:b0506, JW0494
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13616. allR.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi135 – 1428CKSMVRMC → APA: Loss of repressor activity. Loss of DNA binding. Reduces assembly into functionally active tetramers. 1 Publication
Mutagenesisi141 – 1411M → A: No effect on repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication
Mutagenesisi142 – 1421C → W: Strongly reduced repressor activity. Reduces DNA binding. Prevents assembly into functionally active tetramers. 1 Publication
Mutagenesisi215 – 2151L → A: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication
Mutagenesisi217 – 2171C → A or S: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication
Mutagenesisi234 – 2341S → A or N: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication
Mutagenesisi236 – 2361S → A or M: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271HTH-type transcriptional repressor AllRPRO_0000201753Add
BLAST

Proteomic databases

PaxDbiP0ACN4.
PRIDEiP0ACN4.

Interactioni

Subunit structurei

Homotetramer and homodimer. Homotetramer in its active, DNA-bound form. Homodimer in its inactive form.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-561736,EBI-561736
caiCP315523EBI-561736,EBI-1112975

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259871. 6 interactions.
DIPiDIP-47907N.
IntActiP0ACN4. 21 interactions.
STRINGi511145.b0506.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi97 – 11317Combined sources
Beta strandi115 – 1239Combined sources
Beta strandi126 – 1338Combined sources
Beta strandi138 – 1414Combined sources
Beta strandi148 – 1503Combined sources
Turni151 – 1533Combined sources
Helixi155 – 1617Combined sources
Helixi166 – 17611Combined sources
Helixi189 – 20214Combined sources
Beta strandi205 – 2128Combined sources
Beta strandi215 – 2239Combined sources
Beta strandi229 – 23810Combined sources
Turni239 – 2413Combined sources
Helixi244 – 2463Combined sources
Helixi247 – 26519Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TF1X-ray1.80A/B/C/D97-271[»]
ProteinModelPortaliP0ACN4.
SMRiP0ACN4. Positions 23-269.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACN4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 8363HTH iclR-typePROSITE-ProRule annotationAdd
BLAST
Domaini98 – 267170IclR-EDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 1563Glyoxylate binding
Regioni234 – 2363Glyoxylate binding

Sequence similaritiesi

Contains 1 HTH iclR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 iclR-ED (iclR effector binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108N16. Bacteria.
COG1414. LUCA.
HOGENOMiHOG000107041.
InParanoidiP0ACN4.
KOiK10973.
OMAiAPNTITC.
PhylomeDBiP0ACN4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR029016. GAF_dom-like.
IPR014757. Tscrpt_reg_IclR_C.
IPR005471. Tscrpt_reg_IclR_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09339. HTH_IclR. 1 hit.
PF01614. IclR. 1 hit.
[Graphical view]
SMARTiSM00346. HTH_ICLR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS51077. HTH_ICLR. 1 hit.
PS51078. ICLR_ED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVRRRGRP GQAEPVAQKG AQALERGIAI LQYLEKSGGS SSVSDISLNL
60 70 80 90 100
DLPLSTTFRL LKVLQAADFV YQDSQLGWWH IGLGVFNVGA AYIHNRDVLS
110 120 130 140 150
VAGPFMRRLM LLSGETVNVA IRNGNEAVLI GQLECKSMVR MCAPLGSRLP
160 170 180 190 200
LHASGAGKAL LYPLAEEELM SIILQTGLQQ FTPTTLVDMP TLLKDLEQAR
210 220 230 240 250
ELGYTVDKEE HVVGLNCIAS AIYDDVGSVV AAISISGPSS RLTEDRFVSQ
260 270
GELVRDTARD ISTALGLKAH P
Length:271
Mass (Da):29,270
Last modified:November 22, 2005 - v1
Checksum:i476F59EEC0F8308B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89024 Genomic DNA. Translation: AAB93847.1.
U82664 Genomic DNA. Translation: AAB40259.1.
U00096 Genomic DNA. Translation: AAC73608.1.
AP009048 Genomic DNA. Translation: BAE76284.1.
PIRiA64782.
RefSeqiNP_415039.1. NC_000913.3.
WP_000141275.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73608; AAC73608; b0506.
BAE76284; BAE76284; BAE76284.
GeneIDi945333.
KEGGiecj:JW0494.
eco:b0506.
PATRICi32116171. VBIEscCol129921_0527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89024 Genomic DNA. Translation: AAB93847.1.
U82664 Genomic DNA. Translation: AAB40259.1.
U00096 Genomic DNA. Translation: AAC73608.1.
AP009048 Genomic DNA. Translation: BAE76284.1.
PIRiA64782.
RefSeqiNP_415039.1. NC_000913.3.
WP_000141275.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TF1X-ray1.80A/B/C/D97-271[»]
ProteinModelPortaliP0ACN4.
SMRiP0ACN4. Positions 23-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259871. 6 interactions.
DIPiDIP-47907N.
IntActiP0ACN4. 21 interactions.
STRINGi511145.b0506.

Proteomic databases

PaxDbiP0ACN4.
PRIDEiP0ACN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73608; AAC73608; b0506.
BAE76284; BAE76284; BAE76284.
GeneIDi945333.
KEGGiecj:JW0494.
eco:b0506.
PATRICi32116171. VBIEscCol129921_0527.

Organism-specific databases

EchoBASEiEB3382.
EcoGeneiEG13616. allR.

Phylogenomic databases

eggNOGiENOG4108N16. Bacteria.
COG1414. LUCA.
HOGENOMiHOG000107041.
InParanoidiP0ACN4.
KOiK10973.
OMAiAPNTITC.
PhylomeDBiP0ACN4.

Enzyme and pathway databases

BioCyciEcoCyc:G6276-MONOMER.
ECOL316407:JW0494-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACN4.
PROiP0ACN4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR029016. GAF_dom-like.
IPR014757. Tscrpt_reg_IclR_C.
IPR005471. Tscrpt_reg_IclR_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09339. HTH_IclR. 1 hit.
PF01614. IclR. 1 hit.
[Graphical view]
SMARTiSM00346. HTH_ICLR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS51077. HTH_ICLR. 1 hit.
PS51078. ICLR_ED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALLR_ECOLI
AccessioniPrimary (citable) accession number: P0ACN4
Secondary accession number(s): P77734, Q2MBS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.