Skip Header

Contribute Send feedback
Read comments (?) or add your own

P0ACN4 (ALLR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HTH-type transcriptional repressor AllR
Alternative name(s):
Negative regulator of allantoin and glyoxylate utilization operons
Gene names
Name:allR
Synonyms:glxA3, ybbU
Ordered Locus Names:b0506, JW0494
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of allantoin and glyoxylate utilization operons. Binds to the gcl promoter and to the allS-allA intergenic region. Binding to DNA is abolished by glyoxylate. Ref.1 Ref.5 Ref.6

Subunit structure

Homotetramer and homodimer. Homotetramer in its active, DNA-bound form. Homodimer in its inactive form. Ref.6

Sequence similarities

Contains 1 HTH iclR-type DNA-binding domain.

Contains 1 iclR-ED (iclR effector binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271HTH-type transcriptional repressor AllR
PRO_0000201753

Regions

Domain21 – 8363HTH iclR-type
Domain98 – 267170IclR-ED
DNA binding43 – 6220H-T-H motif Potential
Region154 – 1563Glyoxylate binding
Region234 – 2363Glyoxylate binding

Sites

Binding site2071Glyoxylate
Binding site2171Glyoxylate

Experimental info

Mutagenesis135 – 1428CKSMVRMC → APA: Loss of repressor activity. Loss of DNA binding. Reduces assembly into functionally active tetramers. Ref.6
Mutagenesis1411M → A: No effect on repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6
Mutagenesis1421C → W: Strongly reduced repressor activity. Reduces DNA binding. Prevents assembly into functionally active tetramers. Ref.6
Mutagenesis2151L → A: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6
Mutagenesis2171C → A or S: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6
Mutagenesis2341S → A or N: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6
Mutagenesis2361S → A or M: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. Ref.6

Secondary structure

............................ 271
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ACN4 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 476F59EEC0F8308B

FASTA27129,270
        10         20         30         40         50         60 
MTEVRRRGRP GQAEPVAQKG AQALERGIAI LQYLEKSGGS SSVSDISLNL DLPLSTTFRL 

        70         80         90        100        110        120 
LKVLQAADFV YQDSQLGWWH IGLGVFNVGA AYIHNRDVLS VAGPFMRRLM LLSGETVNVA 

       130        140        150        160        170        180 
IRNGNEAVLI GQLECKSMVR MCAPLGSRLP LHASGAGKAL LYPLAEEELM SIILQTGLQQ 

       190        200        210        220        230        240 
FTPTTLVDMP TLLKDLEQAR ELGYTVDKEE HVVGLNCIAS AIYDDVGSVV AAISISGPSS 

       250        260        270 
RLTEDRFVSQ GELVRDTARD ISTALGLKAH P 

« Hide

References

« Hide 'large scale' references
[1]"Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli."
Cusa E., Obradors N., Baldoma L., Badia J., Aguilar J.
J. Bacteriol. 181:7479-7484(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: K12 / ECL1.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Regulation of the Escherichia coli allantoin regulon: coordinated function of the repressor AllR and the activator AllS."
Rintoul M.R., Cusa E., Baldoma L., Badia J., Reitzer L., Aguilar J.
J. Mol. Biol. 324:599-610(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
Strain: K12.
[6]"Structural and biochemical study of effector molecule recognition by the E.coli glyoxylate and allantoin utilization regulatory protein AllR."
Walker J.R., Altamentova S., Ezersky A., Lorca G., Skarina T., Kudritska M., Ball L.J., Bochkarev A., Savchenko A.
J. Mol. Biol. 358:810-828(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 97-271 IN COMPLEX WITH GLYOXALATE, FUNCTION, SUBUNIT, MUTAGENESIS OF 135-CYS--CYS-142; MET-141; CYS-142; LEU-215; CYS-217; SER-234 AND SER-236.
Strain: K12 / BW25113.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89024 Genomic DNA. Translation: AAB93847.1.
U82664 Genomic DNA. Translation: AAB40259.1.
U00096 Genomic DNA. Translation: AAC73608.1.
AP009048 Genomic DNA. Translation: BAE76284.1.
PIRA64782.
RefSeqNP_415039.1. NC_000913.2.
YP_488796.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TF1X-ray1.80A/B/C/D97-271[»]
ProteinModelPortalP0ACN4.
SMRP0ACN4. Positions 23-269.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47907N.
IntActP0ACN4. 20 interactions.
STRING511145.b0506.

Proteomic databases

PaxDbP0ACN4.
PRIDEP0ACN4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73608; AAC73608; b0506.
BAE76284; BAE76284; BAE76284.
GeneID12930877.
945333.
KEGGecj:Y75_p0492.
eco:b0506.
PATRIC32116171. VBIEscCol129921_0527.

Organism-specific databases

EchoBASEEB3382.
EcoGeneEG13616. allR.

Phylogenomic databases

eggNOGCOG1414.
HOGENOMHOG000107041.
KOK10973.
OMAYLHICAS.
ProtClustDBPRK10163.

Enzyme and pathway databases

BioCycEcoCyc:G6276-MONOMER.
ECOL316407:JW0494-MONOMER.

Gene expression databases

GenevestigatorP0ACN4.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR014757. Tscrpt_reg_IclR_C.
IPR005471. Tscrpt_reg_IclR_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF09339. HTH_IclR. 1 hit.
PF01614. IclR. 1 hit.
[Graphical view]
SMARTSM00346. HTH_ICLR. 1 hit.
[Graphical view]
PROSITEPS51077. HTH_ICLR. 1 hit.
PS51078. ICLR_ED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0ACN4.

Entry information

Entry nameALLR_ECOLI
AccessionPrimary (citable) accession number: P0ACN4
Secondary accession number(s): P77734, Q2MBS2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families