P0ACL9 (PDHR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase complex repressor

Gene names

Name:	pdhR
Synonyms:	aceC, genA, yacB
Ordered Locus Names:	b0113, JW0109

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor for the pyruvate dehydrogenase complex genes aceEF and lpd.
Sequence similarities	Contains 1 HTH gntR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 7783622PubMed 8057842Ref.5. Source: EcoCyc positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 7783622PubMed 8057842Ref.5. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 7783622PubMed 8057842Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Pyruvate dehydrogenase complex repressor		PRO_0000050662
Regions
	Domain	9 – 77	69	HTH gntR-type
	DNA binding	37 – 56	20	H-T-H motif By similarity
Natural variations
	Natural variant	118	1	R → C in ACEC816; constitutively activated.

Sequences

	Sequence		Length	Mass (Da)
	P0ACL9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: AF20245D3D3F751D	FASTA	254	29,425
	10 20 30 40 50 60 MAYSKIRQPK LSDVIEQQLE FLILEGTLRP GEKLPPEREL AKQFDVSRPS LREAIQRLEA 70 80 90 100 110 120 KGLLLRRQGG GTFVQSSLWQ SFSDPLVELL SDHPESQYDL LETRHALEGI AAYYAALRST 130 140 150 160 170 180 DEDKERIREL HHAIELAQQS GDLDAESNAV LQYQIAVTEA AHNVVLLHLL RCMEPMLAQN 190 200 210 220 230 240 VRQNFELLYS RREMLPLVSS HRTRIFEAIM AGKPEEAREA SHRHLAFIEE ILLDRSREES 250 RRERSLRRLE QRKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the pyruvate dehydrogenase component." Stephens P.E., Darlison M.G., Lewis H.M., Guest J.R. Eur. J. Biochem. 133:155-162(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	Quail M.A. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A mutation causing constitutive synthesis of the pyruvate dehydrogenase complex in Escherichia coli is located within the pdhR gene." Haydon D.J., Quail M.A., Guest J.R. FEBS Lett. 336:43-47(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, VARIANT ACEC816. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01498 Genomic DNA. Translation: CAA24739.1. U00096 Genomic DNA. Translation: AAC73224.1. AP009048 Genomic DNA. Translation: BAB96683.2. S67363 Genomic DNA. Translation: AAB29356.1.
PIR	BVECA. A64734.
RefSeq	NP_414655.1. NC_000913.2. YP_488416.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACL9.
SMR	P0ACL9. Positions 15-235.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0ACL9. 7 interactions.
STRING	511145.b0113.
Proteomic databases
PaxDb	P0ACL9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73224; AAC73224; b0113. BAB96683; BAB96683; BAB96683.
GeneID	12931667. 944827.
KEGG	ecj:Y75_p0110. eco:b0113.
PATRIC	32115327. VBIEscCol129921_0115.
Organism-specific databases
EchoBASE	EB1080.
EcoGene	EG11088. pdhR.
Phylogenomic databases
eggNOG	COG2186.
HOGENOM	HOG000273991.
KO	K05799.
OMA	RIRDCHA.
ProtClustDB	PRK09464.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11088-MONOMER. ECOL316407:JW0109-MONOMER.
Gene expression databases
Genevestigator	P0ACL9.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011711. GntR_C. IPR000524. Tscrpt_reg_HTH_GntR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF07729. FCD. 1 hit. PF00392. GntR. 1 hit. [Graphical view]
PRINTS	PR00035. HTHGNTR.
SMART	SM00895. FCD. 1 hit. SM00345. HTH_GNTR. 1 hit. [Graphical view]
PROSITE	PS50949. HTH_GNTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDHR_ECOLI

Accession

Primary (citable) accession number: P0ACL9
Secondary accession number(s): P06957, Q53381, Q8KMZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program