Reviewed, UniProtKB/Swiss-Prot P0ACK2 (AGAR_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative aga operon transcriptional repressor

Gene names

Name:	agaR
Synonyms:	yhaW
Ordered Locus Names:	b3131, JW3100

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Probable repressor for the aga operon for N-acetyl galactosamine transport and metabolism.
Sequence similarities	Contains 1 HTH deoR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Putative aga operon transcriptional repressor		PRO_0000050241
Regions
	Domain	15 – 70	56	HTH deoR-type
	DNA binding	32 – 51	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ACK2-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 73B5C19B357AE014	FASTA	269	29,572
	10 20 30 40 50 60 MSNTDASGEK RVTGTSERRE QIIQRLRQQG SVQVNDLSAL YGVSTVTIRN DLAFLEKQGI 70 80 90 100 110 120 AVRAYGGALI CDSTTPSVEP SVEDKSALNT AMKRSVAKAA VELIQPGHRV ILDSGTTTFE 130 140 150 160 170 180 IARLMRKHTD VIAMTNGMNV ANALLEAEGV ELLMTGGHLR RQSQSFYGDQ AEQSLQNYHF 190 200 210 220 230 240 DMLFLGVDAI DLERGVSTHN EDEARLNRRM CEVAERIIVV TDSSKFNRSS LHKIIDTQRI 250 260 DMIIVDEGIP ADSLEGLRKA GVEVILVGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli." Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W. Mol. Microbiol. 37:125-135(2000) [PubMed: 10931310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228498 Genomic DNA. Translation: AAF81081.1. U18997 Genomic DNA. Translation: AAA57934.1. U00096 Genomic DNA. Translation: AAC76165.1. AP009048 Genomic DNA. Translation: BAE77178.1.
PIR	G65102.
RefSeq	AP_003677.1. NP_417600.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	947636.
GenomeReviews	Gene locus JW3100 in contig AP009048_GR. Gene locus b3131 in contig U00096_GR.
KEGG	ecj:JW3100. eco:b3131.
Organism-specific databases
EchoBASE	EB2615.
EcoGene	EG12762. agaR.
CMR	Search...
Phylogenomic databases
HOGENOM	P0ACK2.
OMA	P0ACK2. TTTYEIA.
Enzyme and pathway databases
BioCyc	EcoCyc:G7630-MON.
Family and domain databases
InterPro	IPR014036. HTH_DeoR. IPR001034. HTH_DeoR_N. IPR018356. Tscrpt_reg_HTH_DeoR_CS. [Graphical view]
Pfam	PF00455. DeoR. 1 hit. PF08220. HTH_DeoR. 1 hit. [Graphical view]
SMART	SM00420. HTH_DEOR. 1 hit. [Graphical view]
PROSITE	PS00894. HTH_DEOR_1. 1 hit. PS51000. HTH_DEOR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAR_ECOLI

Accession

Primary (citable) accession number: P0ACK2
Secondary accession number(s): P42902, Q2M978

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)