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P0ACK1

- CRP_SHIFL

UniProt

P0ACK1 - CRP_SHIFL

Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei97 – 971Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Sitei102 – 1021Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi57 – 637cAMP 1By similarity
    Nucleotide bindingi72 – 743cAMP 1By similarity
    Nucleotide bindingi83 – 842cAMP 1By similarity
    Nucleotide bindingi128 – 1292cAMP 1By similarity
    Nucleotide bindingi136 – 1372cAMP 2By similarity
    Nucleotide bindingi171 – 18111cAMP 2By similarityAdd
    BLAST
    DNA bindingi180 – 1867H-T-H motifPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-activated global transcriptional regulator CRP
    Alternative name(s):
    Catabolite activator protein
    Short name:
    CAP
    Catabolite gene activator
    cAMP receptor protein
    Short name:
    CRP
    cAMP regulatory protein
    Gene namesi
    Name:crp
    Ordered Locus Names:SF3376, S4387
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210cAMP-activated global transcriptional regulator CRPPRO_0000100151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei101 – 1011N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP0ACK1.
    PRIDEiP0ACK1.

    Interactioni

    Subunit structurei

    Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi198214.SF3376.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACK1.
    SMRiP0ACK1. Positions 9-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 21073HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 223Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Regioni53 – 597Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity
    Regioni154 – 16310Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity

    Domaini

    The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000250565.
    KOiK10914.
    OMAiLGSQMAE.
    OrthoDBiEOG69GZGV.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV    50
    LIKDEEGKEM ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY 100
    KKFRQLIQVN PDILMRLSAQ MARRLQVTSE KVGNLAFLDV TGRIAQTLLN 150
    LAKQPDAMTH PDGMQIKITR QEIGQIVGCS RETVGRILKM LEDQNLISAH 200
    GKTIVVYGTR 210
    Length:210
    Mass (Da):23,640
    Last modified:July 21, 1986 - v1
    Checksum:iDCBC24FA46C61B3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13772 Genomic DNA. Translation: AAA26515.1.
    AE005674 Genomic DNA. Translation: AAN44839.1.
    AE014073 Genomic DNA. Translation: AAP19339.1.
    RefSeqiNP_709132.1. NC_004337.2.
    NP_839528.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN44839; AAN44839; SF3376.
    AAP19339; AAP19339; S4387.
    GeneIDi1026956.
    1080595.
    KEGGisfl:SF3376.
    sfx:S4387.
    PATRICi18710780. VBIShiFle31049_4794.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13772 Genomic DNA. Translation: AAA26515.1 .
    AE005674 Genomic DNA. Translation: AAN44839.1 .
    AE014073 Genomic DNA. Translation: AAP19339.1 .
    RefSeqi NP_709132.1. NC_004337.2.
    NP_839528.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0ACK1.
    SMRi P0ACK1. Positions 9-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF3376.

    Proteomic databases

    PaxDbi P0ACK1.
    PRIDEi P0ACK1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN44839 ; AAN44839 ; SF3376 .
    AAP19339 ; AAP19339 ; S4387 .
    GeneIDi 1026956.
    1080595.
    KEGGi sfl:SF3376.
    sfx:S4387.
    PATRICi 18710780. VBIShiFle31049_4794.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000250565.
    KOi K10914.
    OMAi LGSQMAE.
    OrthoDBi EOG69GZGV.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli."
      Cossart P., Groisman E.A., Serre M.-C., Casadaban M.J., Gicquel-Sanzey B.
      J. Bacteriol. 167:639-646(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12022 / CDC 3591-52 / Serotype 2b.
    2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiCRP_SHIFL
    AccessioniPrimary (citable) accession number: P0ACK1
    Secondary accession number(s): P03020
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3