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P0ACK1 (CRP_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name=CAP
Catabolite gene activator
cAMP receptor protein
Short name=CRP
cAMP regulatory protein
Gene names
Name:crp
Ordered Locus Names:SF3376, S4387
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) By similarity.

Subunit structure

Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) By similarity.

Domain

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound By similarity.

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210cAMP-activated global transcriptional regulator CRP
PRO_0000100151

Regions

Domain138 – 21073HTH crp-type
Nucleotide binding57 – 637cAMP 1 By similarity
Nucleotide binding72 – 743cAMP 1 By similarity
Nucleotide binding83 – 842cAMP 1 By similarity
Nucleotide binding128 – 1292cAMP 1 By similarity
Nucleotide binding136 – 1372cAMP 2 By similarity
Nucleotide binding171 – 18111cAMP 2 By similarity
DNA binding180 – 1867H-T-H motif By similarity
Region20 – 223Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Region53 – 597Activating region 3 (AR3); probably contacts sigma-70 (RpoD) By similarity
Region154 – 16310Activating region 1 (AR1); probably contacts the C-terminus of RpoA By similarity

Sites

Site971Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Site1021Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity

Amino acid modifications

Modified residue1011N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P0ACK1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: DCBC24FA46C61B3D

FASTA21023,640
        10         20         30         40         50         60 
MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV LIKDEEGKEM 

        70         80         90        100        110        120 
ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY KKFRQLIQVN PDILMRLSAQ 

       130        140        150        160        170        180 
MARRLQVTSE KVGNLAFLDV TGRIAQTLLN LAKQPDAMTH PDGMQIKITR QEIGQIVGCS 

       190        200        210 
RETVGRILKM LEDQNLISAH GKTIVVYGTR 

« Hide

References

« Hide 'large scale' references
[1]"crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli."
Cossart P., Groisman E.A., Serre M.-C., Casadaban M.J., Gicquel-Sanzey B.
J. Bacteriol. 167:639-646(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12022 / CDC 3591-52 / Serotype 2b.
[2]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[3]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13772 Genomic DNA. Translation: AAA26515.1.
AE005674 Genomic DNA. Translation: AAN44839.1.
AE014073 Genomic DNA. Translation: AAP19339.1.
RefSeqNP_709132.1. NC_004337.2.
NP_839528.1. NC_004741.1.

3D structure databases

ProteinModelPortalP0ACK1.
SMRP0ACK1. Positions 9-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198214.SF3376.

Proteomic databases

PaxDbP0ACK1.
PRIDEP0ACK1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN44839; AAN44839; SF3376.
AAP19339; AAP19339; S4387.
GeneID1026956.
1080595.
KEGGsfl:SF3376.
sfx:S4387.
PATRIC18710780. VBIShiFle31049_4794.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000250565.
KOK10914.
OMADQLYFIV.
OrthoDBEOG69GZGV.
ProtClustDBPRK11753.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSPR00034. HTHCRP.
SMARTSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRP_SHIFL
AccessionPrimary (citable) accession number: P0ACK1
Secondary accession number(s): P03020
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families