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P0ACJ9

- CRP_ECOL6

UniProt

P0ACJ9 - CRP_ECOL6

Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei97 – 971Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Sitei102 – 1021Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi57 – 637cAMP 1By similarity
    Nucleotide bindingi72 – 743cAMP 1By similarity
    Nucleotide bindingi83 – 842cAMP 1By similarity
    Nucleotide bindingi128 – 1292cAMP 1By similarity
    Nucleotide bindingi136 – 1372cAMP 2By similarity
    Nucleotide bindingi171 – 18111cAMP 2By similarityAdd
    BLAST
    DNA bindingi180 – 1867H-T-H motifPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL199310:C4132-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-activated global transcriptional regulator CRP
    Alternative name(s):
    Catabolite activator protein
    Short name:
    CAP
    Catabolite gene activator
    cAMP receptor protein
    Short name:
    CRP
    cAMP regulatory protein
    Gene namesi
    Name:crp
    Ordered Locus Names:c4132
    OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
    Taxonomic identifieri199310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001410: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210cAMP-activated global transcriptional regulator CRPPRO_0000100146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei101 – 1011N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP0ACJ9.

    Interactioni

    Subunit structurei

    Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi199310.c4132.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACJ9.
    SMRiP0ACJ9. Positions 9-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 21073HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 223Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Regioni53 – 597Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity
    Regioni154 – 16310Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity

    Domaini

    The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000250565.
    KOiK10914.
    OMAiLGSQMAE.
    OrthoDBiEOG69GZGV.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACJ9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV    50
    LIKDEEGKEM ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY 100
    KKFRQLIQVN PDILMRLSAQ MARRLQVTSE KVGNLAFLDV TGRIAQTLLN 150
    LAKQPDAMTH PDGMQIKITR QEIGQIVGCS RETVGRILKM LEDQNLISAH 200
    GKTIVVYGTR 210
    Length:210
    Mass (Da):23,640
    Last modified:July 21, 1986 - v1
    Checksum:iDCBC24FA46C61B3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN82570.1.
    RefSeqiNP_755996.1. NC_004431.1.

    Genome annotation databases

    EnsemblBacteriaiAAN82570; AAN82570; c4132.
    GeneIDi1036667.
    KEGGiecc:c4132.
    PATRICi18286014. VBIEscCol75197_3883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN82570.1 .
    RefSeqi NP_755996.1. NC_004431.1.

    3D structure databases

    ProteinModelPortali P0ACJ9.
    SMRi P0ACJ9. Positions 9-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 199310.c4132.

    Proteomic databases

    PRIDEi P0ACJ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN82570 ; AAN82570 ; c4132 .
    GeneIDi 1036667.
    KEGGi ecc:c4132.
    PATRICi 18286014. VBIEscCol75197_3883.

    Phylogenomic databases

    HOGENOMi HOG000250565.
    KOi K10914.
    OMAi LGSQMAE.
    OrthoDBi EOG69GZGV.

    Enzyme and pathway databases

    BioCyci ECOL199310:C4132-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFT073 / ATCC 700928 / UPEC.

    Entry informationi

    Entry nameiCRP_ECOL6
    AccessioniPrimary (citable) accession number: P0ACJ9
    Secondary accession number(s): P03020
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3