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P0ACJ0

- LRP_ECOLI

UniProt

P0ACJ0 - LRP_ECOLI

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Protein

Leucine-responsive regulatory protein

Gene
lrp, alsB, ihb, livR, oppI, b0889, JW0872
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 5020H-T-H motif Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. protein binding Source: IntAct
  3. sequence-specific DNA binding Source: EcoliWiki
  4. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. leucine biosynthetic process Source: EcoCyc
  2. regulation of transcription, DNA-templated Source: EcoliWiki
  3. response to leucine Source: EcoliWiki
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10547. lrp.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 164163Leucine-responsive regulatory proteinPRO_0000111728Add
BLAST

Proteomic databases

PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Expressioni

Gene expression databases

GenevestigatoriP0ACJ0.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1113316,EBI-1113316
rnkP0AFW44EBI-1113316,EBI-553769

Protein-protein interaction databases

DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactions.
STRINGi511145.b0889.

Structurei

Secondary structure

1
164
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Helixi15 – 2511
Helixi33 – 353
Beta strandi37 – 393
Turni42 – 443
Beta strandi45 – 473
Helixi49 – 557
Beta strandi56 – 6510
Turni67 – 715
Beta strandi75 – 817
Helixi89 – 979
Beta strandi103 – 12018
Helixi124 – 1307
Turni131 – 1333
Beta strandi138 – 1403
Beta strandi142 – 15514

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0. Positions 8-164.

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7362HTH asnC-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1522.
HOGENOMiHOG000115327.
KOiK03719.
OMAiFAIAQIS.
OrthoDBiEOG6F29D2.
PhylomeDBiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0-1 [UniParc]FASTAAdd to Basket

« Hide

MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR    50
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL 100
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM 150
EEVKQSNRLV IKTR 164
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti114 – 1141D → E in lrp-1 mutant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.
YP_489161.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi12931703.
949051.
KEGGiecj:Y75_p0861.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1 .
D11105 Genomic DNA. Translation: BAA01880.1 .
U00096 Genomic DNA. Translation: AAC73975.1 .
AP009048 Genomic DNA. Translation: BAA35614.1 .
PIRi JH0412. RGECLR.
RefSeqi NP_415409.1. NC_000913.3.
YP_489161.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GQQ X-ray 3.20 A/B/C/D 2-164 [» ]
2L4A NMR - A 1-66 [» ]
ProteinModelPortali P0ACJ0.
SMRi P0ACJ0. Positions 8-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35825N.
IntActi P0ACJ0. 12 interactions.
STRINGi 511145.b0889.

Proteomic databases

PaxDbi P0ACJ0.
PRIDEi P0ACJ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73975 ; AAC73975 ; b0889 .
BAA35614 ; BAA35614 ; BAA35614 .
GeneIDi 12931703.
949051.
KEGGi ecj:Y75_p0861.
eco:b0889.
PATRICi 32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASEi EB0542.
EcoGenei EG10547. lrp.

Phylogenomic databases

eggNOGi COG1522.
HOGENOMi HOG000115327.
KOi K03719.
OMAi FAIAQIS.
OrthoDBi EOG6F29D2.
PhylomeDBi P0ACJ0.

Enzyme and pathway databases

BioCyci EcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ACJ0.
PROi P0ACJ0.

Gene expression databases

Genevestigatori P0ACJ0.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProi IPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view ]
PRINTSi PR00033. HTHASNC.
SMARTi SM00344. HTH_ASNC. 1 hit.
[Graphical view ]
SUPFAMi SSF54909. SSF54909. 1 hit.
PROSITEi PS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine."
    Willins D.A., Ryan C., Platko J.V., Calvo J.M.
    J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Ito K., Kawakami K., Nakamura Y.
    Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The ilvIH operon of Escherichia coli is positively regulated."
    Platko J.V., Willins D.A., Calvo J.M.
    J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
  7. "Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli."
    Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.
    J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "The leucine-responsive regulatory protein: more than a regulator?"
    D'Ari R., Lin R.T., Newman E.B.
    Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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