Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0ACJ0

- LRP_ECOLI

UniProt

P0ACJ0 - LRP_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 5020H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. sequence-specific DNA binding Source: EcoliWiki
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. leucine biosynthetic process Source: EcoCyc
  2. regulation of transcription, DNA-templated Source: EcoliWiki
  3. response to leucine Source: EcoliWiki
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10547. lrp.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 164163Leucine-responsive regulatory proteinPRO_0000111728Add
BLAST

Proteomic databases

PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Expressioni

Gene expression databases

GenevestigatoriP0ACJ0.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1113316,EBI-1113316
rnkP0AFW44EBI-1113316,EBI-553769

Protein-protein interaction databases

DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactions.
STRINGi511145.b0889.

Structurei

Secondary structure

1
164
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Helixi15 – 2511
Helixi33 – 353
Beta strandi37 – 393
Turni42 – 443
Beta strandi45 – 473
Helixi49 – 557
Beta strandi56 – 6510
Turni67 – 715
Beta strandi75 – 817
Helixi89 – 979
Beta strandi103 – 12018
Helixi124 – 1307
Turni131 – 1333
Beta strandi138 – 1403
Beta strandi142 – 15514

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0. Positions 8-164.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7362HTH asnC-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH asnC-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1522.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
OMAiFAIAQIS.
OrthoDBiEOG6F29D2.
PhylomeDBiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR
60 70 80 90 100
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL
110 120 130 140 150
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM
160
EEVKQSNRLV IKTR
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti114 – 1141D → E in lrp-1 mutant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.
YP_489161.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi12931703.
949051.
KEGGiecj:Y75_p0861.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1 .
D11105 Genomic DNA. Translation: BAA01880.1 .
U00096 Genomic DNA. Translation: AAC73975.1 .
AP009048 Genomic DNA. Translation: BAA35614.1 .
PIRi JH0412. RGECLR.
RefSeqi NP_415409.1. NC_000913.3.
YP_489161.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GQQ X-ray 3.20 A/B/C/D 2-164 [» ]
2L4A NMR - A 1-66 [» ]
ProteinModelPortali P0ACJ0.
SMRi P0ACJ0. Positions 8-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35825N.
IntActi P0ACJ0. 12 interactions.
STRINGi 511145.b0889.

Proteomic databases

PaxDbi P0ACJ0.
PRIDEi P0ACJ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73975 ; AAC73975 ; b0889 .
BAA35614 ; BAA35614 ; BAA35614 .
GeneIDi 12931703.
949051.
KEGGi ecj:Y75_p0861.
eco:b0889.
PATRICi 32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASEi EB0542.
EcoGenei EG10547. lrp.

Phylogenomic databases

eggNOGi COG1522.
HOGENOMi HOG000115327.
InParanoidi P0ACJ0.
KOi K03719.
OMAi FAIAQIS.
OrthoDBi EOG6F29D2.
PhylomeDBi P0ACJ0.

Enzyme and pathway databases

BioCyci EcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ACJ0.
PROi P0ACJ0.

Gene expression databases

Genevestigatori P0ACJ0.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProi IPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view ]
PRINTSi PR00033. HTHASNC.
SMARTi SM00344. HTH_ASNC. 1 hit.
[Graphical view ]
SUPFAMi SSF54909. SSF54909. 1 hit.
PROSITEi PS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine."
    Willins D.A., Ryan C., Platko J.V., Calvo J.M.
    J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Ito K., Kawakami K., Nakamura Y.
    Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The ilvIH operon of Escherichia coli is positively regulated."
    Platko J.V., Willins D.A., Calvo J.M.
    J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
  7. "Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli."
    Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.
    J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "The leucine-responsive regulatory protein: more than a regulator?"
    D'Ari R., Lin R.T., Newman E.B.
    Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3