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P0ACJ0

- LRP_ECOLI

UniProt

P0ACJ0 - LRP_ECOLI

Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi31 – 5020H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: EcoliWiki
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. leucine biosynthetic process Source: EcoCyc
    2. regulation of transcription, DNA-templated Source: EcoliWiki
    3. response to leucine Source: EcoliWiki
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PD00353.
    ECOL316407:JW0872-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine-responsive regulatory protein
    Gene namesi
    Name:lrp
    Synonyms:alsB, ihb, livR, oppI
    Ordered Locus Names:b0889, JW0872
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10547. lrp.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 164163Leucine-responsive regulatory proteinPRO_0000111728Add
    BLAST

    Proteomic databases

    PaxDbiP0ACJ0.
    PRIDEiP0ACJ0.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACJ0.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1113316,EBI-1113316
    rnkP0AFW44EBI-1113316,EBI-553769

    Protein-protein interaction databases

    DIPiDIP-35825N.
    IntActiP0ACJ0. 12 interactions.
    STRINGi511145.b0889.

    Structurei

    Secondary structure

    1
    164
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 113
    Helixi15 – 2511
    Helixi33 – 353
    Beta strandi37 – 393
    Turni42 – 443
    Beta strandi45 – 473
    Helixi49 – 557
    Beta strandi56 – 6510
    Turni67 – 715
    Beta strandi75 – 817
    Helixi89 – 979
    Beta strandi103 – 12018
    Helixi124 – 1307
    Turni131 – 1333
    Beta strandi138 – 1403
    Beta strandi142 – 15514

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GQQX-ray3.20A/B/C/D2-164[»]
    2L4ANMR-A1-66[»]
    ProteinModelPortaliP0ACJ0.
    SMRiP0ACJ0. Positions 8-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ACJ0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 7362HTH asnC-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH asnC-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1522.
    HOGENOMiHOG000115327.
    KOiK03719.
    OMAiFAIAQIS.
    OrthoDBiEOG6F29D2.
    PhylomeDBiP0ACJ0.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.30.70.920. 1 hit.
    InterProiIPR000485. AsnC-type_HTH_dom.
    IPR011008. Dimeric_a/b-barrel.
    IPR019888. Tscrpt_reg_AsnC-typ.
    IPR019887. Tscrpt_reg_AsnC-typ_C.
    IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01037. AsnC_trans_reg. 1 hit.
    PF13412. HTH_24. 1 hit.
    [Graphical view]
    PRINTSiPR00033. HTHASNC.
    SMARTiSM00344. HTH_ASNC. 1 hit.
    [Graphical view]
    SUPFAMiSSF54909. SSF54909. 1 hit.
    PROSITEiPS00519. HTH_ASNC_1. 1 hit.
    PS50956. HTH_ASNC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0ACJ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR    50
    RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL 100
    EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM 150
    EEVKQSNRLV IKTR 164
    Length:164
    Mass (Da):18,887
    Last modified:January 23, 2007 - v2
    Checksum:i2952FB9347A2725C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti114 – 1141D → E in lrp-1 mutant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35869 Genomic DNA. Translation: AAA24089.1.
    D11105 Genomic DNA. Translation: BAA01880.1.
    U00096 Genomic DNA. Translation: AAC73975.1.
    AP009048 Genomic DNA. Translation: BAA35614.1.
    PIRiJH0412. RGECLR.
    RefSeqiNP_415409.1. NC_000913.3.
    YP_489161.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73975; AAC73975; b0889.
    BAA35614; BAA35614; BAA35614.
    GeneIDi12931703.
    949051.
    KEGGiecj:Y75_p0861.
    eco:b0889.
    PATRICi32116989. VBIEscCol129921_0919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35869 Genomic DNA. Translation: AAA24089.1 .
    D11105 Genomic DNA. Translation: BAA01880.1 .
    U00096 Genomic DNA. Translation: AAC73975.1 .
    AP009048 Genomic DNA. Translation: BAA35614.1 .
    PIRi JH0412. RGECLR.
    RefSeqi NP_415409.1. NC_000913.3.
    YP_489161.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GQQ X-ray 3.20 A/B/C/D 2-164 [» ]
    2L4A NMR - A 1-66 [» ]
    ProteinModelPortali P0ACJ0.
    SMRi P0ACJ0. Positions 8-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35825N.
    IntActi P0ACJ0. 12 interactions.
    STRINGi 511145.b0889.

    Proteomic databases

    PaxDbi P0ACJ0.
    PRIDEi P0ACJ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73975 ; AAC73975 ; b0889 .
    BAA35614 ; BAA35614 ; BAA35614 .
    GeneIDi 12931703.
    949051.
    KEGGi ecj:Y75_p0861.
    eco:b0889.
    PATRICi 32116989. VBIEscCol129921_0919.

    Organism-specific databases

    EchoBASEi EB0542.
    EcoGenei EG10547. lrp.

    Phylogenomic databases

    eggNOGi COG1522.
    HOGENOMi HOG000115327.
    KOi K03719.
    OMAi FAIAQIS.
    OrthoDBi EOG6F29D2.
    PhylomeDBi P0ACJ0.

    Enzyme and pathway databases

    BioCyci EcoCyc:PD00353.
    ECOL316407:JW0872-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0ACJ0.
    PROi P0ACJ0.

    Gene expression databases

    Genevestigatori P0ACJ0.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.30.70.920. 1 hit.
    InterProi IPR000485. AsnC-type_HTH_dom.
    IPR011008. Dimeric_a/b-barrel.
    IPR019888. Tscrpt_reg_AsnC-typ.
    IPR019887. Tscrpt_reg_AsnC-typ_C.
    IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01037. AsnC_trans_reg. 1 hit.
    PF13412. HTH_24. 1 hit.
    [Graphical view ]
    PRINTSi PR00033. HTHASNC.
    SMARTi SM00344. HTH_ASNC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    PROSITEi PS00519. HTH_ASNC_1. 1 hit.
    PS50956. HTH_ASNC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine."
      Willins D.A., Ryan C., Platko J.V., Calvo J.M.
      J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Ito K., Kawakami K., Nakamura Y.
      Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The ilvIH operon of Escherichia coli is positively regulated."
      Platko J.V., Willins D.A., Calvo J.M.
      J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
    7. "Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli."
      Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.
      J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. "The leucine-responsive regulatory protein: more than a regulator?"
      D'Ari R., Lin R.T., Newman E.B.
      Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    9. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiLRP_ECOLI
    AccessioniPrimary (citable) accession number: P0ACJ0
    Secondary accession number(s): P19494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3