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Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 5020H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: EcoliWiki
  • sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  • leucine biosynthetic process Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoliWiki
  • response to leucine Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10547. lrp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 164163Leucine-responsive regulatory proteinPRO_0000111728Add
BLAST

Proteomic databases

PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1113316,EBI-1113316
rnkP0AFW44EBI-1113316,EBI-553769

Protein-protein interaction databases

DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactions.
STRINGi511145.b0889.

Structurei

Secondary structure

1
164
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113Combined sources
Helixi15 – 2511Combined sources
Helixi33 – 353Combined sources
Beta strandi37 – 393Combined sources
Turni42 – 443Combined sources
Beta strandi45 – 473Combined sources
Helixi49 – 557Combined sources
Beta strandi56 – 6510Combined sources
Turni67 – 715Combined sources
Beta strandi75 – 817Combined sources
Helixi89 – 979Combined sources
Beta strandi103 – 12018Combined sources
Helixi124 – 1307Combined sources
Turni131 – 1333Combined sources
Beta strandi138 – 1403Combined sources
Beta strandi142 – 15514Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0. Positions 8-164.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7362HTH asnC-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH asnC-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1522.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
OMAiLVFVEIK.
OrthoDBiEOG6F29D2.
PhylomeDBiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR
60 70 80 90 100
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL
110 120 130 140 150
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM
160
EEVKQSNRLV IKTR
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti114 – 1141D → E in lrp-1 mutant.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi949051.
KEGGiecj:Y75_p0861.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0. Positions 8-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactions.
STRINGi511145.b0889.

Proteomic databases

PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi949051.
KEGGiecj:Y75_p0861.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASEiEB0542.
EcoGeneiEG10547. lrp.

Phylogenomic databases

eggNOGiCOG1522.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
OMAiLVFVEIK.
OrthoDBiEOG6F29D2.
PhylomeDBiP0ACJ0.

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.
PROiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine."
    Willins D.A., Ryan C., Platko J.V., Calvo J.M.
    J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Ito K., Kawakami K., Nakamura Y.
    Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The ilvIH operon of Escherichia coli is positively regulated."
    Platko J.V., Willins D.A., Calvo J.M.
    J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
  7. "Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli."
    Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.
    J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "The leucine-responsive regulatory protein: more than a regulator?"
    D'Ari R., Lin R.T., Newman E.B.
    Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.