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P0ACJ0 (LRP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine-responsive regulatory protein
Gene names
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length164 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Subunit structure

Homodimer.

Sequence similarities

Contains 1 HTH asnC-type DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-1113316,EBI-1113316
rnkP0AFW44EBI-1113316,EBI-553769

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 164163Leucine-responsive regulatory protein
PRO_0000111728

Regions

Domain12 – 7362HTH asnC-type
DNA binding31 – 5020H-T-H motif Potential

Natural variations

Natural variant1141D → E in lrp-1 mutant.

Secondary structure

.............................. 164
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ACJ0 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2952FB9347A2725C

FASTA16418,887
        10         20         30         40         50         60 
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR RLERQGFIQG 

        70         80         90        100        110        120 
YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL EEIQECHLVS GDFDYLLKTR 

       130        140        150        160 
VPDMSAYRKL LGETLLRLPG VNDTRTYVVM EEVKQSNRLV IKTR 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine."
Willins D.A., Ryan C., Platko J.V., Calvo J.M.
J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Ito K., Kawakami K., Nakamura Y.
Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The ilvIH operon of Escherichia coli is positively regulated."
Platko J.V., Willins D.A., Calvo J.M.
J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
[7]"Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli."
Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.
J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"The leucine-responsive regulatory protein: more than a regulator?"
D'Ari R., Lin R.T., Newman E.B.
Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRRGECLR. JH0412.
RefSeqNP_415409.1. NC_000913.3.
YP_489161.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-163[»]
2L4ANMR-A1-66[»]
ProteinModelPortalP0ACJ0.
SMRP0ACJ0. Positions 8-164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35825N.
IntActP0ACJ0. 12 interactions.
STRING511145.b0889.

Proteomic databases

PaxDbP0ACJ0.
PRIDEP0ACJ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneID12931703.
949051.
KEGGecj:Y75_p0861.
eco:b0889.
PATRIC32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASEEB0542.
EcoGeneEG10547. lrp.

Phylogenomic databases

eggNOGCOG1522.
HOGENOMHOG000115327.
KOK03719.
OMAITPCIER.
OrthoDBEOG6F29D2.
PhylomeDBP0ACJ0.
ProtClustDBPRK11169.

Enzyme and pathway databases

BioCycEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Gene expression databases

GenevestigatorP0ACJ0.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01037. AsnC_trans_reg. 1 hit.
PF13412. HTH_24. 1 hit.
[Graphical view]
PRINTSPR00033. HTHASNC.
SMARTSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMSSF54909. SSF54909. 1 hit.
PROSITEPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0ACJ0.
PROP0ACJ0.

Entry information

Entry nameLRP_ECOLI
AccessionPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene