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Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 50H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • alanine catabolic process Source: GO_Central
  • leucine biosynthetic process Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoliWiki
  • response to leucine Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10547. lrp.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001117282 – 164Leucine-responsive regulatory proteinAdd BLAST163

Proteomic databases

EPDiP0ACJ0.
PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000840.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1113316,EBI-1113316
rnkP0AFW44EBI-1113316,EBI-553769

Protein-protein interaction databases

BioGridi4260697. 7 interactors.
DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactors.
STRINGi511145.b0889.

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi15 – 25Combined sources11
Helixi33 – 35Combined sources3
Beta strandi37 – 39Combined sources3
Turni42 – 44Combined sources3
Beta strandi45 – 47Combined sources3
Helixi49 – 55Combined sources7
Beta strandi56 – 65Combined sources10
Turni67 – 71Combined sources5
Beta strandi75 – 81Combined sources7
Helixi89 – 97Combined sources9
Beta strandi103 – 120Combined sources18
Helixi124 – 130Combined sources7
Turni131 – 133Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi142 – 155Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 73HTH asnC-typePROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 1 HTH asnC-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1522. LUCA.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
OMAiLVFVEIK.
PhylomeDBiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC/Lrp_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR
60 70 80 90 100
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL
110 120 130 140 150
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM
160
EEVKQSNRLV IKTR
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114D → E in lrp-1 mutant. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.
WP_000228473.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi949051.
KEGGiecj:JW0872.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.
WP_000228473.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260697. 7 interactors.
DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactors.
STRINGi511145.b0889.

Proteomic databases

EPDiP0ACJ0.
PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi949051.
KEGGiecj:JW0872.
eco:b0889.
PATRICi32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASEiEB0542.
EcoGeneiEG10547. lrp.

Phylogenomic databases

eggNOGiCOG1522. LUCA.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
OMAiLVFVEIK.
PhylomeDBiP0ACJ0.

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.
PROiP0ACJ0.

Gene expression databases

CollecTFiEXPREG_00000840.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC/Lrp_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.