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Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 50H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding Source: CollecTF
  • bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding Source: CollecTF
  • DNA binding transcription factor activity Source: GO_Central
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: EcoliWiki
  • transcription regulatory region sequence-specific DNA binding Source: CollecTF

GO - Biological processi

  • alanine catabolic process Source: GO_Central
  • leucine biosynthetic process Source: EcoCyc
  • negative regulation of transcription, DNA-templated Source: CollecTF
  • regulation of transcription, DNA-templated Source: EcoliWiki
  • response to leucine Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00353

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10547 lrp

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • protein-DNA complex Source: CollecTF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001117282 – 164Leucine-responsive regulatory proteinAdd BLAST163

Proteomic databases

EPDiP0ACJ0
PaxDbiP0ACJ0
PRIDEiP0ACJ0

Expressioni

Gene expression databases

CollecTFiEXPREG_00000840

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260697, 19 interactors
DIPiDIP-35825N
IntActiP0ACJ0, 12 interactors
STRINGi316385.ECDH10B_0959

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi15 – 25Combined sources11
Helixi33 – 35Combined sources3
Beta strandi37 – 39Combined sources3
Turni42 – 44Combined sources3
Beta strandi45 – 47Combined sources3
Helixi49 – 55Combined sources7
Beta strandi56 – 65Combined sources10
Turni67 – 71Combined sources5
Beta strandi75 – 81Combined sources7
Helixi89 – 97Combined sources9
Beta strandi103 – 120Combined sources18
Helixi124 – 130Combined sources7
Turni131 – 133Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi142 – 155Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0
SMRiP0ACJ0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACJ0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 73HTH asnC-typePROSITE-ProRule annotationAdd BLAST62

Phylogenomic databases

eggNOGiCOG1522 LUCA
HOGENOMiHOG000115327
InParanoidiP0ACJ0
KOiK03719
OMAiNCYRITG
PhylomeDBiP0ACJ0

Family and domain databases

CDDicd00090 HTH_ARSR, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011991 ArsR-like_HTH
IPR000485 AsnC-type_HTH_dom
IPR011008 Dimeric_a/b-barrel
IPR019888 Tscrpt_reg_AsnC-typ
IPR019887 Tscrpt_reg_AsnC/Lrp_C
IPR019885 Tscrpt_reg_HTH_AsnC-type_CS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01037 AsnC_trans_reg, 1 hit
PRINTSiPR00033 HTHASNC
SMARTiView protein in SMART
SM00344 HTH_ASNC, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS00519 HTH_ASNC_1, 1 hit
PS50956 HTH_ASNC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR
60 70 80 90 100
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL
110 120 130 140 150
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM
160
EEVKQSNRLV IKTR
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114D → E in lrp-1 mutant. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA Translation: AAA24089.1
D11105 Genomic DNA Translation: BAA01880.1
U00096 Genomic DNA Translation: AAC73975.1
AP009048 Genomic DNA Translation: BAA35614.1
PIRiJH0412 RGECLR
RefSeqiNP_415409.1, NC_000913.3
WP_000228473.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889
BAA35614; BAA35614; BAA35614
GeneIDi32442276
949051
KEGGiecj:JW0872
eco:b0889
PATRICifig|1411691.4.peg.1388

Similar proteinsi

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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