P0ACJ0 (LRP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-responsive regulatory protein

Gene names

Name:	lrp
Synonyms:	alsB, ihb, livR, oppI
Ordered Locus Names:	b0889, JW0872

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.
Subunit structure	Homodimer.
Sequence similarities	Contains 1 HTH asnC-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from mutant phenotype PubMed 12054800. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 12374860. Source: EcoliWiki response to leucine Inferred from mutant phenotype PubMed 1346534. Source: EcoliWiki transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 10551881. Source: EcoliWiki sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-1113316,EBI-1113316

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 164

163

Leucine-responsive regulatory protein

PRO_0000111728

Regions

Domain

12 – 73

62

HTH asnC-type

DNA binding

31 – 50

20

H-T-H motif Potential

Natural variations

Natural variant

114

1

D → E in lrp-1 mutant.

Secondary structure

1

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164

Helix Strand Turn

Details...

Helix

9 – 11

3

Helix

15 – 25

11

Helix

33 – 35

3

Beta strand

37 – 39

3

Turn

42 – 44

3

Beta strand

45 – 47

3

Helix

49 – 55

7

Beta strand

56 – 65

10

Turn

67 – 71

5

Beta strand

75 – 81

7

Helix

89 – 97

9

Beta strand

103 – 120

18

Helix

124 – 130

7

Turn

131 – 133

3

Beta strand

138 – 140

3

Beta strand

142 – 155

14

Sequences

	Sequence		Length	Mass (Da)
	P0ACJ0 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2952FB9347A2725C	FASTA	164	18,887
	10 20 30 40 50 60 MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR RLERQGFIQG 70 80 90 100 110 120 YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL EEIQECHLVS GDFDYLLKTR 130 140 150 160 VPDMSAYRKL LGETLLRLPG VNDTRTYVVM EEVKQSNRLV IKTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine." Willins D.A., Ryan C., Platko J.V., Calvo J.M. J. Biol. Chem. 266:10768-10774(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Ito K., Kawakami K., Nakamura Y. Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The ilvIH operon of Escherichia coli is positively regulated." Platko J.V., Willins D.A., Calvo J.M. J. Bacteriol. 172:4563-4570(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
[7]	"Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli." Haney S.A., Platko J.V., Oxender D.L., Calvo J.M. J. Bacteriol. 174:108-115(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"The leucine-responsive regulatory protein: more than a regulator?" D'Ari R., Lin R.T., Newman E.B. Trends Biochem. Sci. 18:260-263(1993) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.

PIR

RGECLR. JH0412.

RefSeq

NP_415409.1. NC_000913.2.
YP_489161.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GQQ	X-ray	3.20	A/B/C/D	2-163	[»]
2L4A	NMR	-	A	1-66	[»]

ProteinModelPortal

P0ACJ0.

SMR

P0ACJ0. Positions 8-164.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35825N.

IntAct

P0ACJ0. 8 interactions.

STRING

511145.b0889.

Proteomic databases

PaxDb

P0ACJ0.

PRIDE

P0ACJ0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.

GeneID

12931703.
949051.

KEGG

ecj:Y75_p0861.
eco:b0889.

PATRIC

32116989. VBIEscCol129921_0919.

Organism-specific databases

EchoBASE

EB0542.

EcoGene

EG10547. lrp.

Phylogenomic databases

eggNOG

COG1522.

HOGENOM

HOG000115327.

KO

K03719.

OMA

PAIMECH.

ProtClustDB

PRK11169.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00353.
ECOL316407:JW0872-MONOMER.

Gene expression databases

Genevestigator

P0ACJ0.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.
3.30.70.920. 1 hit.

InterPro

IPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC-typ_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF01037. AsnC_trans_reg. 1 hit.
[Graphical view]

PRINTS

PR00033. HTHASNC.

SMART

SM00344. HTH_ASNC. 1 hit.
[Graphical view]

SUPFAM

SSF54909. Dimer_A_B_barrel. 1 hit.

PROSITE

PS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ACJ0.

Entry information

Entry name

LRP_ECOLI

Accession

Primary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program