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Protein

Leucine-responsive regulatory protein

Gene

lrp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons; lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 50H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding Source: CollecTF
  • bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding Source: CollecTF
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: EcoliWiki
  • transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • transcription regulatory region sequence-specific DNA binding Source: CollecTF

GO - Biological processi

  • alanine catabolic process Source: GO_Central
  • leucine biosynthetic process Source: EcoCyc
  • negative regulation of transcription, DNA-templated Source: CollecTF
  • regulation of transcription, DNA-templated Source: EcoliWiki
  • response to leucine Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00353.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-responsive regulatory protein
Gene namesi
Name:lrp
Synonyms:alsB, ihb, livR, oppI
Ordered Locus Names:b0889, JW0872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10547. lrp.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • protein-DNA complex Source: CollecTF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001117282 – 164Leucine-responsive regulatory proteinAdd BLAST163

Proteomic databases

PaxDbiP0ACJ0.
PRIDEiP0ACJ0.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000840.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260697. 7 interactors.
DIPiDIP-35825N.
IntActiP0ACJ0. 12 interactors.
STRINGi316385.ECDH10B_0959.

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi15 – 25Combined sources11
Helixi33 – 35Combined sources3
Beta strandi37 – 39Combined sources3
Turni42 – 44Combined sources3
Beta strandi45 – 47Combined sources3
Helixi49 – 55Combined sources7
Beta strandi56 – 65Combined sources10
Turni67 – 71Combined sources5
Beta strandi75 – 81Combined sources7
Helixi89 – 97Combined sources9
Beta strandi103 – 120Combined sources18
Helixi124 – 130Combined sources7
Turni131 – 133Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi142 – 155Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GQQX-ray3.20A/B/C/D2-164[»]
2L4ANMR-A1-66[»]
ProteinModelPortaliP0ACJ0.
SMRiP0ACJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 73HTH asnC-typePROSITE-ProRule annotationAdd BLAST62

Phylogenomic databases

eggNOGiCOG1522. LUCA.
HOGENOMiHOG000115327.
InParanoidiP0ACJ0.
KOiK03719.
PhylomeDBiP0ACJ0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC/Lrp_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01037. AsnC_trans_reg. 1 hit.
PRINTSiPR00033. HTHASNC.
SMARTiView protein in SMART
SM00344. HTH_ASNC. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiView protein in PROSITE
PS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR
60 70 80 90 100
RLERQGFIQG YTALLNPHYL DASLLVFVEI TLNRGAPDVF EQFNTAVQKL
110 120 130 140 150
EEIQECHLVS GDFDYLLKTR VPDMSAYRKL LGETLLRLPG VNDTRTYVVM
160
EEVKQSNRLV IKTR
Length:164
Mass (Da):18,887
Last modified:January 23, 2007 - v2
Checksum:i2952FB9347A2725C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti114D → E in lrp-1 mutant. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35869 Genomic DNA. Translation: AAA24089.1.
D11105 Genomic DNA. Translation: BAA01880.1.
U00096 Genomic DNA. Translation: AAC73975.1.
AP009048 Genomic DNA. Translation: BAA35614.1.
PIRiJH0412. RGECLR.
RefSeqiNP_415409.1. NC_000913.3.
WP_000228473.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73975; AAC73975; b0889.
BAA35614; BAA35614; BAA35614.
GeneIDi32442276.
949051.
KEGGiecj:JW0872.
eco:b0889.
PATRICifig|1411691.4.peg.1388.

Similar proteinsi

Entry informationi

Entry nameiLRP_ECOLI
AccessioniPrimary (citable) accession number: P0ACJ0
Secondary accession number(s): P19494
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references