Right origin-binding protein - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0ACI0 (ROB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Right origin-binding protein

Gene names

Name:	rob
Ordered Locus Names:	b4396, JW4359

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication.
Sequence similarities	Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 10551881. Source: EcoliWiki sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 289

289

Right origin-binding protein

PRO_0000194578

Regions

DNA binding

24 – 43

H-T-H motif By similarity

Secondary structure

289

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ACI0 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 4436B1A04BE86A93
Show »

FASTA

289

33,145

        10         20         30         40         50         60 
MDQAGIIRDL LIWLEGHLDQ PLSLDNVAAK AGYSKWHLQR MFKDVTGHAI GAYIRARRLS 

        70         80         90        100        110        120 
KSAVALRLTA RPILDIALQY RFDSQQTFTR AFKKQFAQTP ALYRRSPEWS AFGIRPPLRL 

       130        140        150        160        170        180 
GEFTMPEHKF VTLEDTPLIG VTQSYSCSLE QISDFRHEMR YQFWHDFLGN APTIPPVLYG 

       190        200        210        220        230        240 
LNETRPSQDK DDEQEVFYTT ALAQDQADGY VLTGHPVMLQ GGEYVMFTYE GLGTGVQEFI 

       250        260        270        280 
LTVYGTCMPM LNLTRRKGQD IERYYPAEDA KAGDRPINLR CELLIPIRR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel binding protein of the origin of the Escherichia coli chromosome." Skarstad K., Thoeny B., Hwang D.S., Kornberg A. J. Biol. Chem. 268:5365-5370(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M97495 Genomic DNA. Translation: AAA24569.1.
U14003 Genomic DNA. Translation: AAA97292.1.
U00096 Genomic DNA. Translation: AAC77349.1.
AP009048 Genomic DNA. Translation: BAE78385.1.

PIR

JU0158.

RefSeq

NP_418813.1. NC_000913.2.
YP_492526.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D5Y	X-ray	2.70	A/B/C/D	3-289	[»]

ProteinModelPortal

P0ACI0.

SMR

P0ACI0. Positions 3-289.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47868N.

IntAct

P0ACI0. 6 interactions.

MINT

MINT-1246646.

STRING

511145.b4396.

Protein family/group databases

AraC-XylS

Search...

Proteomic databases

PaxDb

P0ACI0.

PRIDE

P0ACI0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77349; AAC77349; b4396.
BAE78385; BAE78385; BAE78385.

GeneID

12930327.
948916.

KEGG

ecj:Y75_p4280.
eco:b4396.

PATRIC

32124410. VBIEscCol129921_4545.

Organism-specific databases

EchoBASE

EB1340.

EcoGene

EG11366. rob.

Phylogenomic databases

eggNOG

COG2207.

HOGENOM

HOG000127886.

K05804.

OMA

RMFKDIT.

ProtClustDB

PRK15121.

Enzyme and pathway databases

BioCyc

EcoCyc:PD04418.
ECOL316407:JW4359-MONOMER.

Gene expression databases

Genevestigator

P0ACI0.

Family and domain databases

Gene3D

1.10.10.60. 2 hits.

InterPro

IPR010499. AraC_E-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR011256. Reg_factor_effector_bac.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]

Pfam

PF06445. GyrI-like. 1 hit.
[Graphical view]

PRINTS

PR00032. HTHARAC.

SMART

SM00871. AraC_E_bind. 1 hit.
SM00342. HTH_ARAC. 1 hit.
[Graphical view]

SUPFAM

SSF55136. Bac_reg_effector. 1 hit.
SSF46689. Homeodomain_like. 2 hits.

PROSITE

PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ACI0.

Entry information

Entry name

ROB_ECOLI

Accession

Primary (citable) accession number: P0ACI0
Secondary accession number(s): P27292, Q2M5S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents