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Protein

Right origin-binding protein

Gene

rob

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi24 – 4320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD04418.
ECOL316407:JW4359-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Right origin-binding protein
Gene namesi
Name:rob
Ordered Locus Names:b4396, JW4359
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11366. rob.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Right origin-binding proteinPRO_0000194578Add
BLAST

Proteomic databases

PaxDbiP0ACI0.
PRIDEiP0ACI0.

Interactioni

Protein-protein interaction databases

BioGridi4259321. 106 interactions.
DIPiDIP-47868N.
IntActiP0ACI0. 6 interactions.
MINTiMINT-1246646.
STRINGi511145.b4396.

Structurei

Secondary structure

1
289
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512Combined sources
Beta strandi18 – 214Combined sources
Helixi25 – 284Combined sources
Turni29 – 313Combined sources
Helixi35 – 4612Combined sources
Helixi50 – 6819Combined sources
Helixi73 – 797Combined sources
Helixi85 – 9612Combined sources
Helixi100 – 1056Combined sources
Beta strandi107 – 1104Combined sources
Beta strandi128 – 1336Combined sources
Beta strandi136 – 1449Combined sources
Helixi149 – 1513Combined sources
Helixi152 – 17019Combined sources
Beta strandi178 – 1869Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi194 – 20310Combined sources
Helixi204 – 2074Combined sources
Helixi208 – 2103Combined sources
Beta strandi215 – 2195Combined sources
Beta strandi222 – 23211Combined sources
Helixi233 – 2353Combined sources
Helixi236 – 24510Combined sources
Helixi247 – 2504Combined sources
Beta strandi260 – 2645Combined sources
Helixi266 – 2683Combined sources
Beta strandi278 – 28811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5YX-ray2.70A/B/C/D3-289[»]
ProteinModelPortaliP0ACI0.
SMRiP0ACI0. Positions 3-289.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACI0.

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105UEI. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000127886.
InParanoidiP0ACI0.
KOiK05804.
OMAiWCATGIC.
OrthoDBiEOG62C9DF.
PhylomeDBiP0ACI0.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.20.80.10. 1 hit.
InterProiIPR010499. AraC_E-bd.
IPR029442. GyrI-like.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR011256. Reg_factor_effector_dom.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF06445. GyrI-like. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00871. AraC_E_bind. 1 hit.
SM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF55136. SSF55136. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQAGIIRDL LIWLEGHLDQ PLSLDNVAAK AGYSKWHLQR MFKDVTGHAI
60 70 80 90 100
GAYIRARRLS KSAVALRLTA RPILDIALQY RFDSQQTFTR AFKKQFAQTP
110 120 130 140 150
ALYRRSPEWS AFGIRPPLRL GEFTMPEHKF VTLEDTPLIG VTQSYSCSLE
160 170 180 190 200
QISDFRHEMR YQFWHDFLGN APTIPPVLYG LNETRPSQDK DDEQEVFYTT
210 220 230 240 250
ALAQDQADGY VLTGHPVMLQ GGEYVMFTYE GLGTGVQEFI LTVYGTCMPM
260 270 280
LNLTRRKGQD IERYYPAEDA KAGDRPINLR CELLIPIRR
Length:289
Mass (Da):33,145
Last modified:November 22, 2005 - v1
Checksum:i4436B1A04BE86A93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97495 Genomic DNA. Translation: AAA24569.1.
U14003 Genomic DNA. Translation: AAA97292.1.
U00096 Genomic DNA. Translation: AAC77349.1.
AP009048 Genomic DNA. Translation: BAE78385.1.
PIRiJU0158.
RefSeqiNP_418813.1. NC_000913.3.
WP_000371666.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77349; AAC77349; b4396.
BAE78385; BAE78385; BAE78385.
GeneIDi948916.
KEGGiecj:JW4359.
eco:b4396.
PATRICi32124410. VBIEscCol129921_4545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97495 Genomic DNA. Translation: AAA24569.1.
U14003 Genomic DNA. Translation: AAA97292.1.
U00096 Genomic DNA. Translation: AAC77349.1.
AP009048 Genomic DNA. Translation: BAE78385.1.
PIRiJU0158.
RefSeqiNP_418813.1. NC_000913.3.
WP_000371666.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5YX-ray2.70A/B/C/D3-289[»]
ProteinModelPortaliP0ACI0.
SMRiP0ACI0. Positions 3-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259321. 106 interactions.
DIPiDIP-47868N.
IntActiP0ACI0. 6 interactions.
MINTiMINT-1246646.
STRINGi511145.b4396.

Proteomic databases

PaxDbiP0ACI0.
PRIDEiP0ACI0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77349; AAC77349; b4396.
BAE78385; BAE78385; BAE78385.
GeneIDi948916.
KEGGiecj:JW4359.
eco:b4396.
PATRICi32124410. VBIEscCol129921_4545.

Organism-specific databases

EchoBASEiEB1340.
EcoGeneiEG11366. rob.

Phylogenomic databases

eggNOGiENOG4105UEI. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000127886.
InParanoidiP0ACI0.
KOiK05804.
OMAiWCATGIC.
OrthoDBiEOG62C9DF.
PhylomeDBiP0ACI0.

Enzyme and pathway databases

BioCyciEcoCyc:PD04418.
ECOL316407:JW4359-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACI0.
PROiP0ACI0.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.20.80.10. 1 hit.
InterProiIPR010499. AraC_E-bd.
IPR029442. GyrI-like.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR011256. Reg_factor_effector_dom.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF06445. GyrI-like. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00871. AraC_E_bind. 1 hit.
SM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF55136. SSF55136. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel binding protein of the origin of the Escherichia coli chromosome."
    Skarstad K., Thoeny B., Hwang D.S., Kornberg A.
    J. Biol. Chem. 268:5365-5370(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiROB_ECOLI
AccessioniPrimary (citable) accession number: P0ACI0
Secondary accession number(s): P27292, Q2M5S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 13, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.