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Protein

Melibiose operon regulatory protein

Gene

melR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator for the expression of the melAB operon. MelR binds at two sites located upstream of the melAB transcription site.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi210 – 22920H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00208.
ECOL316407:JW4079-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Melibiose operon regulatory protein
Gene namesi
Name:melR
Ordered Locus Names:b4118, JW4079
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11230. melR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi222 – 2221N → A: No effect. 1 Publication
Mutagenesisi269 – 2691R → G: No effect. 1 Publication
Mutagenesisi271 – 2711S → A: 50% reduced activity. 1 Publication
Mutagenesisi272 – 2721S → N: 75% reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Melibiose operon regulatory proteinPRO_0000194537Add
BLAST

Proteomic databases

PaxDbiP0ACH8.
PRIDEiP0ACH8.

Interactioni

Protein-protein interaction databases

BioGridi4262686. 12 interactions.
DIPiDIP-10181N.
IntActiP0ACH8. 14 interactions.
STRINGi511145.b4118.

Structurei

3D structure databases

ProteinModelPortaliP0ACH8.
SMRiP0ACH8. Positions 193-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108K2B. Bacteria.
ENOG410XP2B. LUCA.
HOGENOMiHOG000127063.
InParanoidiP0ACH8.
OMAiMCLFWGG.
PhylomeDBiP0ACH8.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR008917. TF_DNA-bd.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47454. SSF47454. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTDTFMCSS DEKQTRSPLS LYSEYQRMEI EFRAPHIMPT SHWHGQVEVN
60 70 80 90 100
VPFDGDVEYL INNEKVNINQ GHITLFWACT PHQLTDTGTC QSMAIFNLPM
110 120 130 140 150
HLFLSWPLDK DLINHVTHGM VIKSLATQQL SPFEVRRWQQ ELNSPNEQIR
160 170 180 190 200
QLAIDEIGLM LKRFSLSGWE PILVNKTSRT HKNSVSRHAQ FYVSQMLGFI
210 220 230 240 250
AENYDQALTI NDVAEHVKLN ANYAMGIFQR VMQLTMKQYI TAMRINHVRA
260 270 280 290 300
LLSDTDKSIL DIALTAGFRS SSRFYSTFGK YVGMSPQQYR KLSQQRRQTF

PG
Length:302
Mass (Da):34,928
Last modified:November 22, 2005 - v1
Checksum:i2D9BC3069AB05029
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18425 Genomic DNA. No translation available.
U14003 Genomic DNA. Translation: AAA97018.1.
U00096 Genomic DNA. Translation: AAC77079.1.
AP009048 Genomic DNA. Translation: BAE78120.1.
PIRiA29625. RGECMB.
RefSeqiNP_418542.1. NC_000913.3.
WP_001090758.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77079; AAC77079; b4118.
BAE78120; BAE78120; BAE78120.
GeneIDi948637.
KEGGiecj:JW4079.
eco:b4118.
PATRICi32123801. VBIEscCol129921_4249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18425 Genomic DNA. No translation available.
U14003 Genomic DNA. Translation: AAA97018.1.
U00096 Genomic DNA. Translation: AAC77079.1.
AP009048 Genomic DNA. Translation: BAE78120.1.
PIRiA29625. RGECMB.
RefSeqiNP_418542.1. NC_000913.3.
WP_001090758.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACH8.
SMRiP0ACH8. Positions 193-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262686. 12 interactions.
DIPiDIP-10181N.
IntActiP0ACH8. 14 interactions.
STRINGi511145.b4118.

Proteomic databases

PaxDbiP0ACH8.
PRIDEiP0ACH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77079; AAC77079; b4118.
BAE78120; BAE78120; BAE78120.
GeneIDi948637.
KEGGiecj:JW4079.
eco:b4118.
PATRICi32123801. VBIEscCol129921_4249.

Organism-specific databases

EchoBASEiEB1212.
EcoGeneiEG11230. melR.

Phylogenomic databases

eggNOGiENOG4108K2B. Bacteria.
ENOG410XP2B. LUCA.
HOGENOMiHOG000127063.
InParanoidiP0ACH8.
OMAiMCLFWGG.
PhylomeDBiP0ACH8.

Enzyme and pathway databases

BioCyciEcoCyc:PD00208.
ECOL316407:JW4079-MONOMER.

Miscellaneous databases

PROiP0ACH8.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR008917. TF_DNA-bd.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47454. SSF47454. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMELR_ECOLI
AccessioniPrimary (citable) accession number: P0ACH8
Secondary accession number(s): P10411, Q2M6I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.