P0ACG4 (HOKC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein hokC

Alternative name(s):
Protein gef

Gene names

Name:	hokC
Synonyms:	gef
Ordered Locus Names:	b4412, JW5002
ORF Names:	b0018.1

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	50 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	When overexpressed, kills the cells from the inside by interfering with a vital function in the cell membrane.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the hok/gef family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 50	50	Protein hokC		PRO_0000199031
Regions
	Topological domain	1 – 5	5	Cytoplasmic Probable
	Transmembrane	6 – 24	19	Helical; Signal-anchor for type II membrane protein; Probable
	Topological domain	25 – 50	26	Periplasmic Probable
Experimental info
	Mutagenesis	15	1	C → S: No action on toxicity.
	Mutagenesis	25	1	T → K: Non-toxic.
	Mutagenesis	29	1	L → P: Non-toxic.
	Mutagenesis	30	1	C → S: Prevents the formation of dimer.
	Mutagenesis	41	1	V → A: Non-toxic.
	Mutagenesis	49	1	S → E: Non-toxic.

Sequences

	Sequence		Length	Mass (Da)
	P0ACG4 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 9EC371A1043F682D	FASTA	50	5,502
	10 20 30 40 50 MKQHKAMIVA LIVICITAVV AALVTRKDLC EVHIRTGQTE VAVFTAYESE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of genes encoding a cell-killing function may be conserved in all Gram-negative bacteria." Poulsen L.K., Larsen N.W., Molin S., Andersson P. Mol. Microbiol. 3:1463-1472(1989) [PubMed: 2693900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Topographic analysis of the toxic Gef protein from Escherichia coli." Poulsen L.K., Refn A., Molin S., Andersson P. Mol. Microbiol. 5:1627-1637(1991) [PubMed: 1943700] [Abstract] Cited for: MUTAGENESIS, TOPOLOGY.
[6]	"Multiple hok genes on the chromosome of Escherichia coli." Pedersen K., Gerdes K. Mol. Microbiol. 32:1090-1102(1999) [PubMed: 10361310] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17311 Genomic DNA. Translation: CAB37348.1. U00096 Genomic DNA. Translation: AAT48122.1. AP009048 Genomic DNA. Translation: BAE76031.1.
RefSeq	YP_025292.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0ACG4.
ModBase	Search...
Protein family/group databases
TCDB	9.B.6.1.1. toxic hok/gef protein (Hok/Gef) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004815; EBESCP00000197397; EBESCG00000003931. EBESCT00000018268; EBESCP00000017559; EBESCG00000017323.
GeneID	2847744.
GenomeReviews	Gene locus JW5002 in contig AP009048_GR. Gene locus b4412 in contig U00096_GR.
KEGG	ecj:JW5002. eco:b4412.
PATRIC	32115127. VBIEscCol129921_0015.
Organism-specific databases
EchoBASE	EB0368.
EcoGene	EG10373. hokC.
Phylogenomic databases
eggNOG	NOG12921.
GeneTree	EBGT00050000009513.
HOGENOM	HBG473224.
ProtClustDB	CLSK2307230.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-1564.
Gene expression databases
Genevestigator	P0ACG4.
Family and domain databases
InterPro	IPR000021. Hok/gef_toxin. IPR018084. Hok/gef_toxin_CS. [Graphical view]
Pfam	PF01848. HOK_GEF. 1 hit. [Graphical view]
PRINTS	PR00281. HOKGEFTOXIC.
ProDom	PD005979. Hok/gef_toxin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00556. HOK_GEF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOKC_ECOLI

Accession

Primary (citable) accession number: P0ACG4
Secondary accession number(s): P22982, P75618, Q2MCH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program