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Protein

Protein HokC

Gene

hokC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

When overexpressed, kills the cells from the inside by interfering with a vital function in the cell membrane.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1564.
ECOL316407:JW5002-MONOMER.

Protein family/group databases

TCDBi1.E.53.1.1. the toxic hok/gef protein (hok/gef) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HokC
Alternative name(s):
Protein gef
Gene namesi
Name:hokC
Synonyms:gef
Ordered Locus Names:b4412, JW5002
ORF Names:b0018.1
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10373. hokC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic1 Publication
Transmembranei6 – 2419Helical; Signal-anchor for type II membrane proteinCuratedAdd
BLAST
Topological domaini25 – 5026Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • periplasmic space Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151C → S: No action on toxicity. 1 Publication
Mutagenesisi25 – 251T → K: Non-toxic. 1 Publication
Mutagenesisi29 – 291L → P: Non-toxic. 1 Publication
Mutagenesisi30 – 301C → S: Prevents the formation of dimer. 1 Publication
Mutagenesisi41 – 411V → A: Non-toxic. 1 Publication
Mutagenesisi49 – 491S → E: Non-toxic. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5050Protein HokCPRO_0000199031Add
BLAST

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0ACG4.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

BioGridi4259470. 3 interactions.
STRINGi511145.b4412.

Structurei

3D structure databases

ProteinModelPortaliP0ACG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hok/gef family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41123E9. LUCA.
HOGENOMiHOG000041219.
KOiK18919.
PhylomeDBiP0ACG4.

Family and domain databases

InterProiIPR000021. Hok/gef_toxin.
IPR018084. Hok/gef_toxin_CS.
[Graphical view]
PfamiPF01848. HOK_GEF. 1 hit.
[Graphical view]
PRINTSiPR00281. HOKGEFTOXIC.
ProDomiPD005979. Hok/gef_toxin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00556. HOK_GEF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQHKAMIVA LIVICITAVV AALVTRKDLC EVHIRTGQTE VAVFTAYESE
Length:50
Mass (Da):5,502
Last modified:November 22, 2005 - v1
Checksum:i9EC371A1043F682D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17311 Genomic DNA. Translation: CAB37348.1.
U00096 Genomic DNA. Translation: AAT48122.1.
AP009048 Genomic DNA. Translation: BAE76031.1.
RefSeqiWP_000809168.1. NZ_LN832404.1.
YP_025292.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48122; AAT48122; b4412.
BAE76031; BAE76031; BAE76031.
GeneIDi2847744.
KEGGiecj:JW5002.
eco:b4412.
PATRICi32115127. VBIEscCol129921_0015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17311 Genomic DNA. Translation: CAB37348.1.
U00096 Genomic DNA. Translation: AAT48122.1.
AP009048 Genomic DNA. Translation: BAE76031.1.
RefSeqiWP_000809168.1. NZ_LN832404.1.
YP_025292.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0ACG4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259470. 3 interactions.
STRINGi511145.b4412.

Protein family/group databases

TCDBi1.E.53.1.1. the toxic hok/gef protein (hok/gef) family.

Proteomic databases

PaxDbiP0ACG4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48122; AAT48122; b4412.
BAE76031; BAE76031; BAE76031.
GeneIDi2847744.
KEGGiecj:JW5002.
eco:b4412.
PATRICi32115127. VBIEscCol129921_0015.

Organism-specific databases

EchoBASEiEB0368.
EcoGeneiEG10373. hokC.

Phylogenomic databases

eggNOGiENOG41123E9. LUCA.
HOGENOMiHOG000041219.
KOiK18919.
PhylomeDBiP0ACG4.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1564.
ECOL316407:JW5002-MONOMER.

Miscellaneous databases

PROiP0ACG4.

Family and domain databases

InterProiIPR000021. Hok/gef_toxin.
IPR018084. Hok/gef_toxin_CS.
[Graphical view]
PfamiPF01848. HOK_GEF. 1 hit.
[Graphical view]
PRINTSiPR00281. HOKGEFTOXIC.
ProDomiPD005979. Hok/gef_toxin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00556. HOK_GEF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOKC_ECOLI
AccessioniPrimary (citable) accession number: P0ACG4
Secondary accession number(s): P22982, P75618, Q2MCH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.