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Protein

DNA-binding protein HU-beta

Gene

hupB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

GO - Biological processi

  • chromosome condensation Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10467-MONOMER.
ECOL316407:JW0430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-beta
Alternative name(s):
HU-1
NS1
Gene namesi
Name:hupB
Synonyms:hopD
Ordered Locus Names:b0440, JW0430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10467. hupB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30A → D: No effect. 1 Publication1
Mutagenesisi37K → Q: No effect. 1 Publication1
Mutagenesisi47F → T: Reduced DNA-binding. 1 Publication1
Mutagenesisi58 – 61RTGR → GTGG: Reduced DNA-binding. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001049391 – 90DNA-binding protein HU-betaAdd BLAST90

Proteomic databases

EPDiP0ACF4.
PaxDbiP0ACF4.
PRIDEiP0ACF4.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-370411,EBI-370411
hupAP0ACF06EBI-370411,EBI-547648

Protein-protein interaction databases

BioGridi4260733. 44 interactors.
DIPiDIP-31833N.
IntActiP0ACF4. 68 interactors.
MINTiMINT-1236756.
STRINGi511145.b0440.

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Helixi18 – 37Combined sources20
Beta strandi42 – 44Combined sources3
Turni45 – 47Combined sources3
Beta strandi48 – 54Combined sources7
Beta strandi75 – 81Combined sources7
Helixi83 – 88Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O97X-ray2.45B1-90[»]
4P3VX-ray1.25A1-90[»]
ProteinModelPortaliP0ACF4.
SMRiP0ACF4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF4.
KOiK03530.
OMAiGSWSVSK.
PhylomeDBiP0ACF4.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKSQLIDKI AAGADISKAA AGRALDAIIA SVTESLKEGD DVALVGFGTF
60 70 80 90
AVKERAARTG RNPQTGKEIT IAAAKVPSFR AGKALKDAVN
Length:90
Mass (Da):9,226
Last modified:July 21, 1986 - v1
Checksum:iC56A3C766D53BB96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12A → E AA sequence (PubMed:350619).Curated1
Sequence conflicti16I → L AA sequence (PubMed:350619).Curated1
Sequence conflicti26D → E AA sequence (PubMed:350619).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16540 Genomic DNA. Translation: CAA34539.1.
U82664 Genomic DNA. Translation: AAB40196.1.
U00096 Genomic DNA. Translation: AAC73543.1.
AP009048 Genomic DNA. Translation: BAE76220.1.
X53241 Genomic DNA. Translation: CAA37332.1.
D82943 Genomic DNA. Translation: BAA11644.1.
PIRiS06880. DNECS1.
RefSeqiNP_414974.1. NC_000913.3.
WP_001043542.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73543; AAC73543; b0440.
BAE76220; BAE76220; BAE76220.
GeneIDi949095.
KEGGiecj:JW0430.
eco:b0440.
PATRICi32116033. VBIEscCol129921_0458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16540 Genomic DNA. Translation: CAA34539.1.
U82664 Genomic DNA. Translation: AAB40196.1.
U00096 Genomic DNA. Translation: AAC73543.1.
AP009048 Genomic DNA. Translation: BAE76220.1.
X53241 Genomic DNA. Translation: CAA37332.1.
D82943 Genomic DNA. Translation: BAA11644.1.
PIRiS06880. DNECS1.
RefSeqiNP_414974.1. NC_000913.3.
WP_001043542.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O97X-ray2.45B1-90[»]
4P3VX-ray1.25A1-90[»]
ProteinModelPortaliP0ACF4.
SMRiP0ACF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260733. 44 interactors.
DIPiDIP-31833N.
IntActiP0ACF4. 68 interactors.
MINTiMINT-1236756.
STRINGi511145.b0440.

Proteomic databases

EPDiP0ACF4.
PaxDbiP0ACF4.
PRIDEiP0ACF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73543; AAC73543; b0440.
BAE76220; BAE76220; BAE76220.
GeneIDi949095.
KEGGiecj:JW0430.
eco:b0440.
PATRICi32116033. VBIEscCol129921_0458.

Organism-specific databases

EchoBASEiEB0462.
EcoGeneiEG10467. hupB.

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF4.
KOiK03530.
OMAiGSWSVSK.
PhylomeDBiP0ACF4.

Enzyme and pathway databases

BioCyciEcoCyc:EG10467-MONOMER.
ECOL316407:JW0430-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACF4.
PROiP0ACF4.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBHB_ECOLI
AccessioniPrimary (citable) accession number: P0ACF4
Secondary accession number(s): P02341, Q2MBY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.