DNA-binding protein HU-beta - Escherichia coli (strain K12)

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P0ACF4 (DBHB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA-binding protein HU-beta

Alternative name(s):
HU-1
NS1

Gene names

Name:	hupB
Synonyms:	hopD
Ordered Locus Names:	b0440, JW0430

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
Subunit structure	Heterodimer of an alpha and a beta chain. Ref.12
Sequence similarities	Belongs to the bacterial histone-like protein family.

Ontologies

Keywords
Biological process	DNA condensation
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
hupA	P0ACF0	2	EBI-370411,EBI-547648

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

DNA-binding protein HU-beta

PRO_0000104939

Experimental info

Mutagenesis

A → D: No effect.

Mutagenesis

K → Q: No effect.

Mutagenesis

F → T: Reduced DNA-binding.

Mutagenesis

58 – 61

RTGR → GTGG: Reduced DNA-binding.

Sequence conflict

A → E AA sequence Ref.7

Sequence conflict

I → L AA sequence Ref.7

Sequence conflict

D → E AA sequence Ref.7

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ACF4 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C56A3C766D53BB96
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FASTA

9,226

        10         20         30         40         50         60 
MNKSQLIDKI AAGADISKAA AGRALDAIIA SVTESLKEGD DVALVGFGTF AVKERAARTG 

        70         80         90 
RNPQTGKEIT IAAAKVPSFR AGKALKDAVN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli." Kano Y., Yoshimno S., Wada M., Yokoyama K., Nobuhara M., Imamoto F. Mol. Gen. Genet. 201:360-362(1985) [PubMed: 3003540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli." Mende L., Timm B., Subramanian A.R. FEBS Lett. 96:395-398(1978) [PubMed: 215461] [Abstract] Cited for: PROTEIN SEQUENCE.
[6]	"Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli." Laine B., Kmiecik D., Sautiere P., Biserte G., Cohen-Solal M. Eur. J. Biochem. 103:447-461(1980) [PubMed: 6987059] [Abstract] Cited for: PROTEIN SEQUENCE.
[7]	"The amino- and carboxy-terminal amino acid sequences of protein HU from Escherichia coli." Laine B., Sautiere P., Biserte G., Cohen-Solal M., Gros F., Rouviere-Yaniv J. FEBS Lett. 89:116-120(1978) [PubMed: 350619] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-37 AND 80-90.
[8]	"Promoters and autogenous control of the Escherichia coli hupA and hupB genes." Kohno K., Wada M., Kano Y., Imamoto F. J. Mol. Biol. 213:27-36(1990) [PubMed: 2187099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[9]	Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 37-90. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[11]	"Small genes/gene-products in Escherichia coli K-12." Wasinger V.C., Humphery-Smith I. FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10. Strain: K12.
[12]	"Native Escherichia coli HU protein is a heterotypic dimer." Rouviere-Yaniv J., Kjeldgaard N.O. FEBS Lett. 106:297-300(1979) [PubMed: 227733] [Abstract] Cited for: SUBUNIT. Strain: K12 / W3350 / ATCC 27020.
[13]	"HU-1 mutants of Escherichia coli deficient in DNA binding." Goshima N., Kohno K., Imamoto F., Kano Y. Gene 96:141-145(1990) [PubMed: 2265752] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X16540 Genomic DNA. Translation: CAA34539.1.
U82664 Genomic DNA. Translation: AAB40196.1.
U00096 Genomic DNA. Translation: AAC73543.1.
AP009048 Genomic DNA. Translation: BAE76220.1.
X53241 Genomic DNA. Translation: CAA37332.1.
D82943 Genomic DNA. Translation: BAA11644.1.

PIR

DNECS1. S06880.

RefSeq

NP_414974.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O97	X-ray	2.45	B	1-90	[»]

ProteinModelPortal

P0ACF4.

SMR

P0ACF4. Positions 1-90.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31833N.

IntAct

P0ACF4. 65 interactions.

MINT

MINT-1236756.

2D gel databases

ECO2DBASE

I011.5. 6TH EDITION.

Proteomic databases

PRIDE

P0ACF4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001148; EBESCP00000001148; EBESCG00000000948.
EBESCT00000001149; EBESCP00000001149; EBESCG00000000948.
EBESCT00000001150; EBESCP00000001150; EBESCG00000000948.
EBESCT00000001151; EBESCP00000001151; EBESCG00000000948.
EBESCT00000015193; EBESCP00000014484; EBESCG00000014253.

GeneID

949095.

GenomeReviews

Gene locus JW0430 in contig AP009048_GR.
Gene locus b0440 in contig U00096_GR.

KEGG

ecj:JW0430.
eco:b0440.

PATRIC

32116033. VBIEscCol129921_0458.

Organism-specific databases

EchoBASE

EB0462.

EcoGene

EG10467. hupB.

Phylogenomic databases

eggNOG

COG0776.

GeneTree

EBGT00050000010314.

HOGENOM

HBG705085.

OMA

NESKTHF.

PhylomeDB

P0ACF4.

ProtClustDB

PRK10664.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10467-MONOMER.

Gene expression databases

Genevestigator

P0ACF4.

Family and domain databases

InterPro

IPR023630. DNA-bd_HU/IHF.
IPR000119. Hist_DNA-bd_bac.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd.
[Graphical view]

Gene3D

G3DSA:4.10.520.10. Hist_DNA_bd_bac. 1 hit.

K03530.

Pfam

PF00216. Bac_DNA_binding. 1 hit.
[Graphical view]

PRINTS

PR01727. DNABINDINGHU.

SMART

SM00411. BHL. 1 hit.
[Graphical view]

SUPFAM

SSF47729. IHF_like_DNA_bnd. 1 hit.

PROSITE

PS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DBHB_ECOLI

Accession

Primary (citable) accession number: P0ACF4
Secondary accession number(s): P02341, Q2MBY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents