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Protein

DNA-binding protein HU-beta

Gene

hupB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

  • chromosome condensation Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10467-MONOMER.
ECOL316407:JW0430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-beta
Alternative name(s):
HU-1
NS1
Gene namesi
Name:hupB
Synonyms:hopD
Ordered Locus Names:b0440, JW0430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10467. hupB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301A → D: No effect. 1 Publication
Mutagenesisi37 – 371K → Q: No effect. 1 Publication
Mutagenesisi47 – 471F → T: Reduced DNA-binding. 1 Publication
Mutagenesisi58 – 614RTGR → GTGG: Reduced DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9090DNA-binding protein HU-betaPRO_0000104939Add
BLAST

Proteomic databases

EPDiP0ACF4.
PaxDbiP0ACF4.
PRIDEiP0ACF4.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-370411,EBI-370411
hupAP0ACF06EBI-370411,EBI-547648

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260733. 44 interactions.
DIPiDIP-31833N.
IntActiP0ACF4. 68 interactions.
MINTiMINT-1236756.
STRINGi511145.b0440.

Structurei

Secondary structure

1
90
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Helixi18 – 3720Combined sources
Beta strandi42 – 443Combined sources
Turni45 – 473Combined sources
Beta strandi48 – 547Combined sources
Beta strandi75 – 817Combined sources
Helixi83 – 886Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O97X-ray2.45B1-90[»]
4P3VX-ray1.25A1-90[»]
ProteinModelPortaliP0ACF4.
SMRiP0ACF4. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF4.
KOiK03530.
OMAiGSWSVSK.
OrthoDBiEOG615VS6.
PhylomeDBiP0ACF4.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKSQLIDKI AAGADISKAA AGRALDAIIA SVTESLKEGD DVALVGFGTF
60 70 80 90
AVKERAARTG RNPQTGKEIT IAAAKVPSFR AGKALKDAVN
Length:90
Mass (Da):9,226
Last modified:July 21, 1986 - v1
Checksum:iC56A3C766D53BB96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121A → E AA sequence (PubMed:350619).Curated
Sequence conflicti16 – 161I → L AA sequence (PubMed:350619).Curated
Sequence conflicti26 – 261D → E AA sequence (PubMed:350619).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16540 Genomic DNA. Translation: CAA34539.1.
U82664 Genomic DNA. Translation: AAB40196.1.
U00096 Genomic DNA. Translation: AAC73543.1.
AP009048 Genomic DNA. Translation: BAE76220.1.
X53241 Genomic DNA. Translation: CAA37332.1.
D82943 Genomic DNA. Translation: BAA11644.1.
PIRiS06880. DNECS1.
RefSeqiNP_414974.1. NC_000913.3.
WP_001043542.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73543; AAC73543; b0440.
BAE76220; BAE76220; BAE76220.
GeneIDi949095.
KEGGiecj:JW0430.
eco:b0440.
PATRICi32116033. VBIEscCol129921_0458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16540 Genomic DNA. Translation: CAA34539.1.
U82664 Genomic DNA. Translation: AAB40196.1.
U00096 Genomic DNA. Translation: AAC73543.1.
AP009048 Genomic DNA. Translation: BAE76220.1.
X53241 Genomic DNA. Translation: CAA37332.1.
D82943 Genomic DNA. Translation: BAA11644.1.
PIRiS06880. DNECS1.
RefSeqiNP_414974.1. NC_000913.3.
WP_001043542.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O97X-ray2.45B1-90[»]
4P3VX-ray1.25A1-90[»]
ProteinModelPortaliP0ACF4.
SMRiP0ACF4. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260733. 44 interactions.
DIPiDIP-31833N.
IntActiP0ACF4. 68 interactions.
MINTiMINT-1236756.
STRINGi511145.b0440.

Proteomic databases

EPDiP0ACF4.
PaxDbiP0ACF4.
PRIDEiP0ACF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73543; AAC73543; b0440.
BAE76220; BAE76220; BAE76220.
GeneIDi949095.
KEGGiecj:JW0430.
eco:b0440.
PATRICi32116033. VBIEscCol129921_0458.

Organism-specific databases

EchoBASEiEB0462.
EcoGeneiEG10467. hupB.

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF4.
KOiK03530.
OMAiGSWSVSK.
OrthoDBiEOG615VS6.
PhylomeDBiP0ACF4.

Enzyme and pathway databases

BioCyciEcoCyc:EG10467-MONOMER.
ECOL316407:JW0430-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACF4.
PROiP0ACF4.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli."
    Kano Y., Yoshimno S., Wada M., Yokoyama K., Nobuhara M., Imamoto F.
    Mol. Gen. Genet. 201:360-362(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli."
    Mende L., Timm B., Subramanian A.R.
    FEBS Lett. 96:395-398(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  6. "Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli."
    Laine B., Kmiecik D., Sautiere P., Biserte G., Cohen-Solal M.
    Eur. J. Biochem. 103:447-461(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  7. "The amino- and carboxy-terminal amino acid sequences of protein HU from Escherichia coli."
    Laine B., Sautiere P., Biserte G., Cohen-Solal M., Gros F., Rouviere-Yaniv J.
    FEBS Lett. 89:116-120(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-37 AND 80-90.
  8. "Promoters and autogenous control of the Escherichia coli hupA and hupB genes."
    Kohno K., Wada M., Kano Y., Imamoto F.
    J. Mol. Biol. 213:27-36(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
  9. Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 37-90.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  10. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  11. "Small genes/gene-products in Escherichia coli K-12."
    Wasinger V.C., Humphery-Smith I.
    FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  12. "Native Escherichia coli HU protein is a heterotypic dimer."
    Rouviere-Yaniv J., Kjeldgaard N.O.
    FEBS Lett. 106:297-300(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: K12 / W3350 / ATCC 27020.
  13. "HU-1 mutants of Escherichia coli deficient in DNA binding."
    Goshima N., Kohno K., Imamoto F., Kano Y.
    Gene 96:141-145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  14. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiDBHB_ECOLI
AccessioniPrimary (citable) accession number: P0ACF4
Secondary accession number(s): P02341, Q2MBY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 13, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.