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Protein

DNA-binding protein HU-alpha

Gene

hupA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • identical protein binding Source: IntAct

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • chromosome condensation Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10466-MONOMER.
ECOL316407:JW3964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-alpha
Alternative name(s):
HU-2
NS2
Gene namesi
Name:hupA
Ordered Locus Names:b4000, JW3964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10466. hupA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • HU-DNA complex Source: CACAO
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9090DNA-binding protein HU-alphaPRO_0000104935Add
BLAST

Proteomic databases

EPDiP0ACF0.
PaxDbiP0ACF0.
PRIDEiP0ACF0.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-547648,EBI-547648
dnaAP030045EBI-547648,EBI-548951
hupBP0ACF46EBI-547648,EBI-370411

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263459. 132 interactions.
DIPiDIP-35892N.
IntActiP0ACF0. 66 interactions.
MINTiMINT-1244407.
STRINGi511145.b4000.

Structurei

Secondary structure

1
90
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1311Combined sources
Helixi18 – 3720Combined sources
Beta strandi42 – 443Combined sources
Turni45 – 473Combined sources
Beta strandi48 – 558Combined sources
Beta strandi74 – 818Combined sources
Helixi83 – 897Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MULX-ray2.30A1-90[»]
2O97X-ray2.45A1-90[»]
ProteinModelPortaliP0ACF0.
SMRiP0ACF0. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF0.
KOiK05787.
OMAiMNSITYE.
OrthoDBiEOG615VS6.
PhylomeDBiP0ACF0.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKEGD AVQLVGFGTF
60 70 80 90
KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK
Length:90
Mass (Da):9,535
Last modified:July 21, 1986 - v1
Checksum:i6B5B8536FDBD13DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 3214TQAKA…TLAAI → AAAGRALDAIIASV AA sequence (PubMed:350619).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05994 Genomic DNA. Translation: CAA29412.1.
U00006 Genomic DNA. Translation: AAC43098.1.
U00096 Genomic DNA. Translation: AAC76974.1.
AP009048 Genomic DNA. Translation: BAE77319.1.
X54555 Genomic DNA. Translation: CAA38426.1.
D12624 Genomic DNA. Translation: BAA02149.1.
PIRiS06269. DNECS2.
RefSeqiNP_418428.1. NC_000913.3.
WP_001044513.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76974; AAC76974; b4000.
BAE77319; BAE77319; BAE77319.
GeneIDi948499.
KEGGiecj:JW3964.
eco:b4000.
PATRICi32123523. VBIEscCol129921_4114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05994 Genomic DNA. Translation: CAA29412.1.
U00006 Genomic DNA. Translation: AAC43098.1.
U00096 Genomic DNA. Translation: AAC76974.1.
AP009048 Genomic DNA. Translation: BAE77319.1.
X54555 Genomic DNA. Translation: CAA38426.1.
D12624 Genomic DNA. Translation: BAA02149.1.
PIRiS06269. DNECS2.
RefSeqiNP_418428.1. NC_000913.3.
WP_001044513.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MULX-ray2.30A1-90[»]
2O97X-ray2.45A1-90[»]
ProteinModelPortaliP0ACF0.
SMRiP0ACF0. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263459. 132 interactions.
DIPiDIP-35892N.
IntActiP0ACF0. 66 interactions.
MINTiMINT-1244407.
STRINGi511145.b4000.

Proteomic databases

EPDiP0ACF0.
PaxDbiP0ACF0.
PRIDEiP0ACF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76974; AAC76974; b4000.
BAE77319; BAE77319; BAE77319.
GeneIDi948499.
KEGGiecj:JW3964.
eco:b4000.
PATRICi32123523. VBIEscCol129921_4114.

Organism-specific databases

EchoBASEiEB0461.
EcoGeneiEG10466. hupA.

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF0.
KOiK05787.
OMAiMNSITYE.
OrthoDBiEOG615VS6.
PhylomeDBiP0ACF0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10466-MONOMER.
ECOL316407:JW3964-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACF0.
PROiP0ACF0.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the HU-2 gene of Escherichia coli."
    Kano Y., Osato K., Wada M., Imamoto F.
    Mol. Gen. Genet. 209:408-410(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli."
    Mende L., Timm B., Subramanian A.R.
    FEBS Lett. 96:395-398(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  6. "Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli."
    Laine B., Kmiecik D., Sautiere P., Biserte G., Cohen-Solal M.
    Eur. J. Biochem. 103:447-461(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  7. "The amino- and carboxy-terminal amino acid sequences of protein HU from Escherichia coli."
    Laine B., Sautiere P., Biserte G., Cohen-Solal M., Gros F., Rouviere-Yaniv J.
    FEBS Lett. 89:116-120(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-37.
  8. "Promoters and autogenous control of the Escherichia coli hupA and hupB genes."
    Kohno K., Wada M., Kano Y., Imamoto F.
    J. Mol. Biol. 213:27-36(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    Strain: K12.
  9. Nishimura K., Inokuchi H.
    Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
  10. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  11. "Small genes/gene-products in Escherichia coli K-12."
    Wasinger V.C., Humphery-Smith I.
    FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  12. "Native Escherichia coli HU protein is a heterotypic dimer."
    Rouviere-Yaniv J., Kjeldgaard N.O.
    FEBS Lett. 106:297-300(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: K12 / W3350 / ATCC 27020.
  13. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  14. "Chromosome organization by a nucleoid-associated protein in live bacteria."
    Wang W., Li G.W., Chen C., Xie X.S., Zhuang X.
    Science 333:1445-1449(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / BW25993.

Entry informationi

Entry nameiDBHA_ECOLI
AccessioniPrimary (citable) accession number: P0ACF0
Secondary accession number(s): P02342, Q2M8T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.