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Protein

DNA-binding protein HU-alpha

Gene

hupA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • identical protein binding Source: IntAct

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • chromosome condensation Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processDNA condensation

Enzyme and pathway databases

BioCyciEcoCyc:EG10466-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-alpha
Alternative name(s):
HU-2
NS2
Gene namesi
Name:hupA
Ordered Locus Names:b4000, JW3964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10466 hupA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • HU-DNA complex Source: CACAO
  • membrane Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001049351 – 90DNA-binding protein HU-alphaAdd BLAST90

Proteomic databases

EPDiP0ACF0
PaxDbiP0ACF0
PRIDEiP0ACF0

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263459, 132 interactors
DIPiDIP-35892N
IntActiP0ACF0, 77 interactors
STRINGi316385.ECDH10B_4189

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 13Combined sources11
Helixi18 – 37Combined sources20
Beta strandi42 – 44Combined sources3
Turni45 – 47Combined sources3
Beta strandi48 – 55Combined sources8
Beta strandi74 – 81Combined sources8
Helixi83 – 89Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MULX-ray2.30A1-90[»]
2O97X-ray2.45A1-90[»]
4YEWX-ray2.68C1-90[»]
4YEXX-ray3.20A/C1-90[»]
4YEYX-ray3.35A/C1-90[»]
4YF0X-ray2.79A/B1-90[»]
4YFHX-ray3.49A/B1-90[»]
4YFTX-ray2.91C1-90[»]
ProteinModelPortaliP0ACF0
SMRiP0ACF0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF0

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70 Bacteria
COG0776 LUCA
HOGENOMiHOG000043828
InParanoidiP0ACF0
KOiK05787
OMAiERKVPKF
PhylomeDBiP0ACF0

Family and domain databases

Gene3Di4.10.520.10, 1 hit
InterProiView protein in InterPro
IPR000119 Hist_DNA-bd
IPR020816 Histone-like_DNA-bd_CS
IPR010992 IHF-like_DNA-bd_dom_sf
PANTHERiPTHR33175 PTHR33175, 1 hit
PfamiView protein in Pfam
PF00216 Bac_DNA_binding, 1 hit
PRINTSiPR01727 DNABINDINGHU
SMARTiView protein in SMART
SM00411 BHL, 1 hit
SUPFAMiSSF47729 SSF47729, 1 hit
PROSITEiView protein in PROSITE
PS00045 HISTONE_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKEGD AVQLVGFGTF
60 70 80 90
KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK
Length:90
Mass (Da):9,535
Last modified:July 21, 1986 - v1
Checksum:i6B5B8536FDBD13DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 32TQAKA…TLAAI → AAAGRALDAIIASV AA sequence (PubMed:350619).CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05994 Genomic DNA Translation: CAA29412.1
U00006 Genomic DNA Translation: AAC43098.1
U00096 Genomic DNA Translation: AAC76974.1
AP009048 Genomic DNA Translation: BAE77319.1
X54555 Genomic DNA Translation: CAA38426.1
D12624 Genomic DNA Translation: BAA02149.1
PIRiS06269 DNECS2
RefSeqiNP_418428.1, NC_000913.3
WP_001044513.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76974; AAC76974; b4000
BAE77319; BAE77319; BAE77319
GeneIDi948499
KEGGiecj:JW3964
eco:b4000
PATRICifig|1411691.4.peg.2711

Similar proteinsi

Entry informationi

Entry nameiDBHA_ECOLI
AccessioniPrimary (citable) accession number: P0ACF0
Secondary accession number(s): P02342, Q2M8T7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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