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Protein

DNA-binding protein HU-alpha

Gene

hupA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

  • DNA binding Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • chromosome condensation Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA condensation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10466-MONOMER.
ECOL316407:JW3964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-alpha
Alternative name(s):
HU-2
NS2
Gene namesi
Name:hupA
Ordered Locus Names:b4000, JW3964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10466. hupA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • HU-DNA complex Source: CACAO
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001049351 – 90DNA-binding protein HU-alphaAdd BLAST90

Proteomic databases

EPDiP0ACF0.
PaxDbiP0ACF0.
PRIDEiP0ACF0.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-547648,EBI-547648
dnaAP030045EBI-547648,EBI-548951
hupBP0ACF46EBI-547648,EBI-370411

Protein-protein interaction databases

BioGridi4263459. 132 interactors.
DIPiDIP-35892N.
IntActiP0ACF0. 66 interactors.
MINTiMINT-1244407.
STRINGi511145.b4000.

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 13Combined sources11
Helixi18 – 37Combined sources20
Beta strandi42 – 44Combined sources3
Turni45 – 47Combined sources3
Beta strandi48 – 55Combined sources8
Beta strandi74 – 81Combined sources8
Helixi83 – 89Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MULX-ray2.30A1-90[»]
2O97X-ray2.45A1-90[»]
4YEWX-ray2.68C1-90[»]
4YEXX-ray3.20A/C1-90[»]
4YEYX-ray3.35A/C1-90[»]
4YF0X-ray2.79A/B1-90[»]
4YFHX-ray3.49A/B1-90[»]
4YFTX-ray2.91C1-90[»]
ProteinModelPortaliP0ACF0.
SMRiP0ACF0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF0.
KOiK05787.
OMAiMNSITYE.
PhylomeDBiP0ACF0.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKEGD AVQLVGFGTF
60 70 80 90
KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK
Length:90
Mass (Da):9,535
Last modified:July 21, 1986 - v1
Checksum:i6B5B8536FDBD13DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 32TQAKA…TLAAI → AAAGRALDAIIASV AA sequence (PubMed:350619).CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05994 Genomic DNA. Translation: CAA29412.1.
U00006 Genomic DNA. Translation: AAC43098.1.
U00096 Genomic DNA. Translation: AAC76974.1.
AP009048 Genomic DNA. Translation: BAE77319.1.
X54555 Genomic DNA. Translation: CAA38426.1.
D12624 Genomic DNA. Translation: BAA02149.1.
PIRiS06269. DNECS2.
RefSeqiNP_418428.1. NC_000913.3.
WP_001044513.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76974; AAC76974; b4000.
BAE77319; BAE77319; BAE77319.
GeneIDi948499.
KEGGiecj:JW3964.
eco:b4000.
PATRICi32123523. VBIEscCol129921_4114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05994 Genomic DNA. Translation: CAA29412.1.
U00006 Genomic DNA. Translation: AAC43098.1.
U00096 Genomic DNA. Translation: AAC76974.1.
AP009048 Genomic DNA. Translation: BAE77319.1.
X54555 Genomic DNA. Translation: CAA38426.1.
D12624 Genomic DNA. Translation: BAA02149.1.
PIRiS06269. DNECS2.
RefSeqiNP_418428.1. NC_000913.3.
WP_001044513.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MULX-ray2.30A1-90[»]
2O97X-ray2.45A1-90[»]
4YEWX-ray2.68C1-90[»]
4YEXX-ray3.20A/C1-90[»]
4YEYX-ray3.35A/C1-90[»]
4YF0X-ray2.79A/B1-90[»]
4YFHX-ray3.49A/B1-90[»]
4YFTX-ray2.91C1-90[»]
ProteinModelPortaliP0ACF0.
SMRiP0ACF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263459. 132 interactors.
DIPiDIP-35892N.
IntActiP0ACF0. 66 interactors.
MINTiMINT-1244407.
STRINGi511145.b4000.

Proteomic databases

EPDiP0ACF0.
PaxDbiP0ACF0.
PRIDEiP0ACF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76974; AAC76974; b4000.
BAE77319; BAE77319; BAE77319.
GeneIDi948499.
KEGGiecj:JW3964.
eco:b4000.
PATRICi32123523. VBIEscCol129921_4114.

Organism-specific databases

EchoBASEiEB0461.
EcoGeneiEG10466. hupA.

Phylogenomic databases

eggNOGiENOG4105K70. Bacteria.
COG0776. LUCA.
HOGENOMiHOG000043828.
InParanoidiP0ACF0.
KOiK05787.
OMAiMNSITYE.
PhylomeDBiP0ACF0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10466-MONOMER.
ECOL316407:JW3964-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ACF0.
PROiP0ACF0.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBHA_ECOLI
AccessioniPrimary (citable) accession number: P0ACF0
Secondary accession number(s): P02342, Q2M8T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.