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Protein

Purine nucleoside phosphoramidase

Gene

hinT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes purine nucleotide phosphoramidates, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate), guanosine 5'monophosphomorpholidate (GMP-morpholidate) and tryptamine 5'guanosine monophosphate (TpGd) (PubMed:15703176, PubMed:20934431). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine--tRNA ligase, and lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) (PubMed:15703176). Is essential for the activity of the enzyme D-alanine dehydrogenase (DadA) and is required for E.coli to grow on D-alanine as a sole carbon source (PubMed:21754980). Is also required for growth at high salt concentrations (PubMed:15703176).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88Purine nucleotide phosphoramidateIKR:1
Active sitei101Tele-AMP-histidine intermediate2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 32Purine nucleotide phosphoramidateIKR:3
Nucleotide bindingi96 – 97Purine nucleotide phosphoramidateIKR:2
Nucleotide bindingi101 – 103Purine nucleotide phosphoramidateIKR:3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12172-MONOMER.
MetaCyc:EG12172-MONOMER.
SABIO-RKiP0ACE7.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphoramidaseIBD: (EC:3.9.1.-3 Publications)
Alternative name(s):
Histidine triad nucleotide binding protein HinT
Short name:
HIT protein
Gene namesi
Name:hinTIBD:
Synonyms:ycfF
Ordered Locus Names:b1103, JW1089
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12172. hinT.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on both D and L isomers of alanine due to a defect in DadA dehydrogenase activity, preventing D-alanine utilization (PubMed:21754980). The mutant cells do not have appreciable nucleoside phosphoramidase activity (PubMed:21754980, PubMed:15703176). The loss of hinT results in failure to grow in media containing 0.75 M KCl, 0.9 M NaCl, 0.5 M NaOAc, or 10 mM MnCl2 (PubMed:15703176).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101H → A or G: Abolishes enzyme activity. 3 Publications1
Mutagenesisi114 – 119Missing : Strongly reduces enzyme activity. 1 Publication6
Mutagenesisi117 – 119Missing : Abolishes enzyme activity. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098301 – 119Purine nucleoside phosphoramidaseAdd BLAST119

Proteomic databases

PaxDbiP0ACE7.
PRIDEiP0ACE7.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi4261963. 7 interactors.
DIPiDIP-48009N.
IntActiP0ACE7. 14 interactors.
MINTiMINT-1266101.
STRINGi316385.ECDH10B_1175.

Structurei

Secondary structure

1119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11ND:6
Beta strandi19 – 22ND:4
Beta strandi24 – 30ND:7
Beta strandi35 – 46ND:12
Helixi51 – 53ND:3
Helixi56 – 58ND:3
Helixi59 – 75ND:17
Turni79 – 81ND:3
Beta strandi83 – 89ND:7
Helixi90 – 93ND:4
Beta strandi97 – 99ND:3
Beta strandi102 – 108ND:7
Beta strandi113 – 115ND:3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N1SX-ray1.45A/B/E/F/I/J/M/N1-119[»]
3N1TX-ray1.72A/B/E/F1-119[»]
ProteinModelPortaliP0ACE7.
SMRiP0ACE7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACE7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 115HITIEP:Add BLAST110

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi99 – 105Histidine triad motifIKR:7

Sequence similaritiesi

Belongs to the HINT family.IKR:

Phylogenomic databases

eggNOGiENOG4105K59. Bacteria.
COG0537. LUCA.
HOGENOMiHOG000061064.
InParanoidiP0ACE7.
KOiK02503.
PhylomeDBiP0ACE7.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiView protein in InterPro
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR036265. HIT-like_sf.
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiView protein in Pfam
PF01230. HIT. 1 hit.
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiView protein in PROSITE
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ACE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEETIFSKI IRREIPSDIV YQDDLVTAFR DISPQAPTHI LIIPNILIPT
60 70 80 90 100
VNDVSAEHEQ ALGRMITVAA KIAEQEGIAE DGYRLIMNTN RHGGQEVYHI
110
HMHLLGGRPL GPMLAHKGL
Length:119
Mass (Da):13,241
Last modified:November 22, 2005 - v1
Checksum:iF0E6AAF177DC820B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74187.1.
AP009048 Genomic DNA. Translation: BAA35910.1.
X17615 Genomic DNA. No translation available.
PIRiJC5685.
RefSeqiNP_415621.3. NC_000913.3.
WP_000807125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74187; AAC74187; b1103.
BAA35910; BAA35910; BAA35910.
GeneIDi948549.
KEGGiecj:JW1089.
eco:b1103.
PATRICifig|1411691.4.peg.1164.

Similar proteinsi

Entry informationi

Entry nameiHINT_ECOLI
AccessioniPrimary (citable) accession number: P0ACE7
Secondary accession number(s): P36950, P75945
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 25, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families