ID   MBHM_SHIFL              Reviewed;         567 AA.
AC   P0ACE2; P37181;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=Hydrogenase-2 large chain;
DE            Short=HYD2;
DE            EC=1.12.99.6;
DE   AltName: Full=Membrane-bound hydrogenase 2 large subunit;
DE   AltName: Full=NiFe hydrogenase;
DE   Flags: Precursor;
GN   Name=hybC; OrderedLocusNames=SF3041, S3242;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   MEDLINE=22272406; PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H.,
RA   Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J.,
RA   Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S.,
RA   Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y.,
RA   Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   MEDLINE=22590274; PubMed=12704152;
RX   DOI=10.1128/IAI.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W.,
RA   Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A.,
RA   Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S.,
RA   Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella
RT   flexneri serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: This is one of three E.coli hydrogenases synthesized in
CC       response to different physiological conditions. HYD2 is involved
CC       in hydrogen uptake (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein
CC       (By similarity).
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family.
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DR   EMBL; AE005674; AAN44519.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18330.1; -; Genomic_DNA.
DR   RefSeq; NP_708812.1; -.
DR   RefSeq; NP_838520.1; -.
DR   HSSP; P21852; 1UBL.
DR   GeneID; 1026601; -.
DR   GeneID; 1079496; -.
DR   GenomeReviews; AE005674_GR; SF3041.
DR   GenomeReviews; AE014073_GR; S3242.
DR   KEGG; sfl:SF3041; -.
DR   KEGG; sfx:S3242; -.
DR   HOGENOM; P0ACE2; -.
DR   OMA; P0ACE2; HLSALDW.
DR   BioCyc; SFLE198214:AAN44519.1-MON; -.
DR   BRENDA; 1.12.99.6; 189495.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0016151; F:nickel ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Complete proteome; Membrane; Metal-binding; Nickel;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    552       Hydrogenase-2 large chain.
FT                                /FTId=PRO_0000042992.
FT   PROPEP      553    567
FT                                /FTId=PRO_0000042993.
FT   METAL        61     61       Nickel (Potential).
FT   METAL        64     64       Nickel (Potential).
FT   METAL       546    546       Nickel (Potential).
FT   METAL       549    549       Nickel (Potential).
FT   SITE        552    553       Cleavage; by hybD (By similarity).
SQ   SEQUENCE   567 AA;  62491 MW;  A19E6F513390F29E CRC64;
     MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI
     CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT
     SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP
     PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL
     MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP
     GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG
     KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS
     TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG
     MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI
     HSFDPCMACA VHVVDADGNE VVSVKVL
//