ID   MBHM_ECOLI              Reviewed;         567 AA.
AC   P0ACE0; P37181; Q2M9K1;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=Hydrogenase-2 large chain;
DE            Short=HYD2;
DE            EC=1.12.99.6;
DE   AltName: Full=Membrane-bound hydrogenase 2 large subunit;
DE   AltName: Full=NiFe hydrogenase;
DE   Flags: Precursor;
GN   Name=hybC; OrderedLocusNames=b2994, JW2962;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / TG1;
RX   MEDLINE=94292472; PubMed=8021226;
RA   Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C.,
RA   Shanmugam K.T., Peck H.D. Jr., Przybyla A.E.;
RT   "Cloning, sequencing, and mutational analysis of the hyb operon
RT   encoding Escherichia coli hydrogenase 2.";
RL   J. Bacteriol. 176:4416-4423(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-7.
RX   MEDLINE=98409297; PubMed=9738917;
RX   DOI=10.1046/j.1432-1327.1998.2550746.x;
RA   Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H.;
RT   "Reassignment of the gene encoding the Escherichia coli hydrogenase 2
RT   small subunit -- identification of a soluble precursor of the small
RT   subunit in a hypB mutant.";
RL   Eur. J. Biochem. 255:746-754(1998).
CC   -!- FUNCTION: This is one of three E.coli hydrogenases synthesized in
CC       response to different physiological conditions. HYD2 is involved
CC       in hydrogen uptake.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family.
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DR   EMBL; U09177; AAA21591.1; -; Genomic_DNA.
DR   EMBL; U28377; AAA69161.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76030.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77055.1; -; Genomic_DNA.
DR   PIR; C55516; C55516.
DR   RefSeq; AP_003549.1; -.
DR   RefSeq; NP_417468.1; -.
DR   HSSP; P21852; 1UBL.
DR   GeneID; 945182; -.
DR   GenomeReviews; AP009048_GR; JW2962.
DR   GenomeReviews; U00096_GR; b2994.
DR   KEGG; ecj:JW2962; -.
DR   KEGG; eco:b2994; -.
DR   EchoBASE; EB1749; -.
DR   EcoGene; EG11801; hybC.
DR   HOGENOM; P0ACE0; -.
DR   OMA; P0ACE0; HLSALDW.
DR   BioCyc; EcoCyc:HYBC-MON; -.
DR   BioCyc; MetaCyc:HYBC-MON; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0016151; F:nickel ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Complete proteome; Direct protein sequencing; Membrane;
KW   Metal-binding; Nickel; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    552       Hydrogenase-2 large chain.
FT                                /FTId=PRO_0000013407.
FT   PROPEP      553    567
FT                                /FTId=PRO_0000013408.
FT   METAL        61     61       Nickel (Potential).
FT   METAL        64     64       Nickel (Potential).
FT   METAL       546    546       Nickel (Potential).
FT   METAL       549    549       Nickel (Potential).
FT   SITE        552    553       Cleavage; by hybD.
SQ   SEQUENCE   567 AA;  62491 MW;  A19E6F513390F29E CRC64;
     MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI
     CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT
     SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP
     PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL
     MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP
     GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG
     KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS
     TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG
     MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI
     HSFDPCMACA VHVVDADGNE VVSVKVL
//