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Protein

Hydrogenase-2 large chain

Gene

hybC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.

Catalytic activityi

H2 + A = AH2.

Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61NickelSequence analysis1
Metal bindingi64NickelSequence analysis1
Metal bindingi546NickelSequence analysis1
Metal bindingi549NickelSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:HYBC-MONOMER.
ECOL316407:JW2962-MONOMER.
MetaCyc:HYBC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 large chain (EC:1.12.99.6)
Short name:
HYD2
Alternative name(s):
Membrane-bound hydrogenase 2 large subunit
NiFe hydrogenase
Gene namesi
Name:hybC
Ordered Locus Names:b2994, JW2962
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11801. hybC.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of periplasmic side of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000134072 – 552Hydrogenase-2 large chainAdd BLAST551
PropeptideiPRO_0000013408553 – 567Add BLAST15

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei552 – 553Cleavage; by HybD2

Proteomic databases

PaxDbiP0ACE0.
PRIDEiP0ACE0.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi4262375. 11 interactors.
DIPiDIP-36022N.
IntActiP0ACE0. 15 interactors.
MINTiMINT-1254866.
STRINGi511145.b2994.

Structurei

3D structure databases

ProteinModelPortaliP0ACE0.
SMRiP0ACE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DPX. Bacteria.
COG0374. LUCA.
HOGENOMiHOG000278799.
InParanoidiP0ACE0.
KOiK06281.
OMAiKMTAIFG.
PhylomeDBiP0ACE0.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
InterProiIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP
60 70 80 90 100
RDAWMIVQRI CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT
110 120 130 140 150
THDHIVHFYQ LSALDWVDIT SALQADPTKA SEMLKGVSTW HLNSPEEFTK
160 170 180 190 200
VQNKIKDLVA SGQLGIFANG YWGHPAMKLP PEVNLIAVAH YLQALECQRD
210 220 230 240 250
ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL MYIKSFIDKL
260 270 280 290 300
SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP
310 320 330 340 350
GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI
360 370 380 390 400
PAYDGWSDDG KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE
410 420 430 440 450
IVAIYQKLTG NTLEVAQLHS TLGRIIGRTV HCCELQDILQ NQYSALITNI
460 470 480 490 500
GKGDHTTFVK PNIPATGEFK GVGFLEAPRG MLSHWMVIKD GIISNYQAVV
510 520 530 540 550
PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI HSFDPCMACA
560
VHVVDADGNE VVSVKVL
Length:567
Mass (Da):62,491
Last modified:January 23, 2007 - v2
Checksum:iA19E6F513390F29E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21591.1.
U28377 Genomic DNA. Translation: AAA69161.1.
U00096 Genomic DNA. Translation: AAC76030.1.
AP009048 Genomic DNA. Translation: BAE77055.1.
PIRiC55516.
RefSeqiNP_417468.1. NC_000913.3.
WP_000083065.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76030; AAC76030; b2994.
BAE77055; BAE77055; BAE77055.
GeneIDi945182.
KEGGiecj:JW2962.
eco:b2994.
PATRICi32121404. VBIEscCol129921_3089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21591.1.
U28377 Genomic DNA. Translation: AAA69161.1.
U00096 Genomic DNA. Translation: AAC76030.1.
AP009048 Genomic DNA. Translation: BAE77055.1.
PIRiC55516.
RefSeqiNP_417468.1. NC_000913.3.
WP_000083065.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACE0.
SMRiP0ACE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262375. 11 interactors.
DIPiDIP-36022N.
IntActiP0ACE0. 15 interactors.
MINTiMINT-1254866.
STRINGi511145.b2994.

Proteomic databases

PaxDbiP0ACE0.
PRIDEiP0ACE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76030; AAC76030; b2994.
BAE77055; BAE77055; BAE77055.
GeneIDi945182.
KEGGiecj:JW2962.
eco:b2994.
PATRICi32121404. VBIEscCol129921_3089.

Organism-specific databases

EchoBASEiEB1749.
EcoGeneiEG11801. hybC.

Phylogenomic databases

eggNOGiENOG4105DPX. Bacteria.
COG0374. LUCA.
HOGENOMiHOG000278799.
InParanoidiP0ACE0.
KOiK06281.
OMAiKMTAIFG.
PhylomeDBiP0ACE0.

Enzyme and pathway databases

BioCyciEcoCyc:HYBC-MONOMER.
ECOL316407:JW2962-MONOMER.
MetaCyc:HYBC-MONOMER.

Miscellaneous databases

PROiP0ACE0.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
InterProiIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBHM_ECOLI
AccessioniPrimary (citable) accession number: P0ACE0
Secondary accession number(s): P37181, Q2M9K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.