Reviewed, UniProtKB/Swiss-Prot P0ACE0 (MBHM_ECOLI)

Names and origin

Protein names

Recommended name:
    Hydrogenase-2 large chain
      Short name=HYD2
    EC=1.12.99.6
Alternative name(s):
    Membrane-bound hydrogenase 2 large subunit
    NiFe hydrogenase

Gene names

Name:	hybC
Ordered Locus Names:	b2994, JW2962

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 552	551	Hydrogenase-2 large chain		PRO_0000013407
	Propeptide	553 – 567	15			PRO_0000013408
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential
	Metal binding	546	1	Nickel Potential
	Metal binding	549	1	Nickel Potential
	Site	552 – 553	2	Cleavage; by hybD

Sequences

	Sequence		Length	Mass (Da)
	P0ACE0-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A19E6F513390F29E	FASTA	567	62,491
	10 20 30 40 50 60 MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI 70 80 90 100 110 120 CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT 130 140 150 160 170 180 SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP 190 200 210 220 230 240 PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL 250 260 270 280 290 300 MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP 310 320 330 340 350 360 GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG 370 380 390 400 410 420 KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS 430 440 450 460 470 480 TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG 490 500 510 520 530 540 MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI 550 560 HSFDPCMACA VHVVDADGNE VVSVKVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E. J. Bacteriol. 176:4416-4423(1994) [PubMed: 8021226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / TG1.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit -- identification of a soluble precursor of the small subunit in a hypB mutant." Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H. Eur. J. Biochem. 255:746-754(1998) [PubMed: 9738917] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09177 Genomic DNA. Translation: AAA21591.1. U28377 Genomic DNA. Translation: AAA69161.1. U00096 Genomic DNA. Translation: AAC76030.1. AP009048 Genomic DNA. Translation: BAE77055.1.
PIR	C55516.
RefSeq	AP_003549.1. NP_417468.1.
3D structure databases
HSSP	HSSP built from PDB template 1UBL based on UniProtKB P21852.
ModBase	Search...
Genome annotation databases
GeneID	945182.
GenomeReviews	Gene locus JW2962 in contig AP009048_GR. Gene locus b2994 in contig U00096_GR.
KEGG	ecj:JW2962. eco:b2994.
Organism-specific databases
EchoBASE	EB1749.
EcoGene	EG11801. hybC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0ACE0.
OMA	P0ACE0. HLSALDW.
Enzyme and pathway databases
BioCyc	EcoCyc:HYBC-MON. MetaCyc:HYBC-MON.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHM_ECOLI

Accession

Primary (citable) accession number: P0ACE0
Secondary accession number(s): P37181, Q2M9K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)