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Protein

Hydrogenase-2 large chain

Gene

hybC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.

Catalytic activityi

H2 + A = AH2.

Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61NickelSequence analysis1
Metal bindingi64NickelSequence analysis1
Metal bindingi546NickelSequence analysis1
Metal bindingi549NickelSequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:HYBC-MONOMER
MetaCyc:HYBC-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 large chain (EC:1.12.99.6)
Short name:
HYD2
Alternative name(s):
Membrane-bound hydrogenase 2 large subunit
NiFe hydrogenase
Gene namesi
Name:hybC
Ordered Locus Names:b2994, JW2962
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11801 hybC

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of periplasmic side of plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000134072 – 552Hydrogenase-2 large chainAdd BLAST551
PropeptideiPRO_0000013408553 – 567Add BLAST15

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei552 – 553Cleavage; by HybD2

Proteomic databases

PaxDbiP0ACE0
PRIDEiP0ACE0

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
hybGP0AAM72EBI-549849,EBI-562426

Protein-protein interaction databases

BioGridi4262375, 28 interactors
DIPiDIP-36022N
IntActiP0ACE0, 16 interactors
STRINGi316385.ECDH10B_3171

Structurei

3D structure databases

ProteinModelPortaliP0ACE0
SMRiP0ACE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DPX Bacteria
COG0374 LUCA
HOGENOMiHOG000278799
InParanoidiP0ACE0
KOiK06281
OMAiHPAMKLS
PhylomeDBiP0ACE0

Family and domain databases

Gene3Di1.10.645.10, 1 hit
InterProiView protein in InterPro
IPR001501 Ni-dep_hyd_lsu
IPR018194 Ni-dep_hyd_lsu_Ni_BS
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00374 NiFeSe_Hases, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
PROSITEiView protein in PROSITE
PS00507 NI_HGENASE_L_1, 1 hit
PS00508 NI_HGENASE_L_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP
60 70 80 90 100
RDAWMIVQRI CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT
110 120 130 140 150
THDHIVHFYQ LSALDWVDIT SALQADPTKA SEMLKGVSTW HLNSPEEFTK
160 170 180 190 200
VQNKIKDLVA SGQLGIFANG YWGHPAMKLP PEVNLIAVAH YLQALECQRD
210 220 230 240 250
ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL MYIKSFIDKL
260 270 280 290 300
SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP
310 320 330 340 350
GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI
360 370 380 390 400
PAYDGWSDDG KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE
410 420 430 440 450
IVAIYQKLTG NTLEVAQLHS TLGRIIGRTV HCCELQDILQ NQYSALITNI
460 470 480 490 500
GKGDHTTFVK PNIPATGEFK GVGFLEAPRG MLSHWMVIKD GIISNYQAVV
510 520 530 540 550
PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI HSFDPCMACA
560
VHVVDADGNE VVSVKVL
Length:567
Mass (Da):62,491
Last modified:January 23, 2007 - v2
Checksum:iA19E6F513390F29E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA Translation: AAA21591.1
U28377 Genomic DNA Translation: AAA69161.1
U00096 Genomic DNA Translation: AAC76030.1
AP009048 Genomic DNA Translation: BAE77055.1
PIRiC55516
RefSeqiNP_417468.1, NC_000913.3
WP_000083065.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76030; AAC76030; b2994
BAE77055; BAE77055; BAE77055
GeneIDi945182
KEGGiecj:JW2962
eco:b2994
PATRICifig|1411691.4.peg.3735

Similar proteinsi

Entry informationi

Entry nameiMBHM_ECOLI
AccessioniPrimary (citable) accession number: P0ACE0
Secondary accession number(s): P37181, Q2M9K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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