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P0ACD8

- MBHL_ECOLI

UniProt

P0ACD8 - MBHL_ECOLI

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Protein
Hydrogenase-1 large chain
Gene
hyaB, b0973, JW0955
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

Catalytic activityi

H2 + A = AH2.

Cofactori

Binds 1 nickel ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Nickel Reviewed prediction
Metal bindingi79 – 791Nickel Reviewed prediction
Metal bindingi576 – 5761Nickel Reviewed prediction
Metal bindingi579 – 5791Nickel Reviewed prediction

GO - Molecular functioni

  1. ferredoxin hydrogenase activity Source: InterPro
  2. hydrogenase (acceptor) activity Source: UniProtKB-EC
  3. nickel cation binding Source: InterPro
  4. protein binding Source: IntAct
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:HYAB-MONOMER.
ECOL316407:JW0955-MONOMER.
MetaCyc:HYAB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-1 large chain (EC:1.12.99.6)
Short name:
HYD1
Alternative name(s):
Membrane-bound hydrogenase 1 large subunit
NiFe hydrogenase
Gene namesi
Name:hyaB
Ordered Locus Names:b0973, JW0955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10469. hyaB.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Hydrogenase-1 large chain
PRO_0000199712Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0ACD8.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
hyaDP199303EBI-851493,EBI-552940

Protein-protein interaction databases

DIPiDIP-36180N.
IntActiP0ACD8. 8 interactions.
STRINGi511145.b0973.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Beta strandi10 – 134
Beta strandi16 – 216
Beta strandi26 – 294
Beta strandi31 – 377
Beta strandi41 – 5010
Helixi56 – 594
Turni60 – 623
Helixi65 – 673
Helixi68 – 736
Beta strandi77 – 793
Helixi82 – 9514
Helixi101 – 12525
Helixi128 – 1303
Helixi135 – 1395
Helixi142 – 15211
Helixi160 – 17516
Helixi180 – 1823
Helixi196 – 21621
Helixi218 – 2247
Beta strandi225 – 2295
Beta strandi243 – 2453
Helixi248 – 2503
Helixi254 – 27320
Helixi275 – 28511
Helixi287 – 2915
Helixi295 – 2984
Beta strandi301 – 3033
Beta strandi306 – 3105
Turni316 – 3183
Beta strandi319 – 3213
Beta strandi324 – 3263
Turni342 – 3443
Beta strandi345 – 3484
Beta strandi352 – 3543
Helixi365 – 3673
Beta strandi380 – 3823
Beta strandi385 – 3884
Beta strandi400 – 4034
Helixi412 – 42110
Helixi425 – 43713
Helixi442 – 4454
Helixi448 – 47831
Helixi492 – 4943
Beta strandi497 – 50711
Beta strandi510 – 51910
Beta strandi522 – 5298
Helixi531 – 5355
Helixi547 – 5526
Beta strandi560 – 5623
Helixi564 – 5729
Helixi577 – 5815

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47L/M1-582[»]
3USCX-ray2.00L/M1-582[»]
3USEX-ray1.67L/M1-582[»]
4GD3X-ray3.30J/K/L/M1-582[»]
ProteinModelPortaliP0ACD8.
SMRiP0ACD8. Positions 2-582.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0374.
HOGENOMiHOG000278799.
KOiK06281.
OMAiWVEENES.
OrthoDBiEOG6M9DSB.
PhylomeDBiP0ACD8.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
InterProiIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
PROSITEiPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACD8-1 [UniParc]FASTAAdd to Basket

« Hide

MSTQYETQGY TINNAGRRLV VDPITRIEGH MRCEVNINDQ NVITNAVSCG    50
TMFRGLEIIL QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP 100
DNANIIRNIM LATLWCHDHL VHFYQLAGMD WIDVLDALKA DPRKTSELAQ 150
SLSSWPKSSP GYFFDVQNRL KKFVEGGQLG IFRNGYWGHP QYKLPPEANL 200
MGFAHYLEAL DFQREIVKIH AVFGGKNPHP NWIVGGMPCA INIDESGAVG 250
AVNMERLNLV QSIITRTADF INNVMIPDAL AIGQFNKPWS EIGTGLSDKC 300
VLSYGAFPDI ANDFGEKSLL MPGGAVINGD FNNVLPVDLV DPQQVQEFVD 350
HAWYRYPNDQ VGRHPFDGIT DPWYNPGDVK GSDTNIQQLN EQERYSWIKA 400
PRWRGNAMEV GPLARTLIAY HKGDAATVES VDRMMSALNL PLSGIQSTLG 450
RILCRAHEAQ WAAGKLQYFF DKLMTNLKNG NLATASTEKW EPATWPTECR 500
GVGFTEAPRG ALGHWAAIRD GKIDLYQCVV PTTWNASPRD PKGQIGAYEA 550
ALMNTKMAIP EQPLEILRTL HSFDPCLACS THVLGDDGSE LISVQVR 597
Length:597
Mass (Da):66,253
Last modified:November 22, 2005 - v1
Checksum:i659DD7EDE16D6342
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521M → I in AAA23998. 1 Publication
Sequence conflicti69 – 691W → R in AAA23998. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34825 Genomic DNA. Translation: AAA23998.1.
U00096 Genomic DNA. Translation: AAC74058.1.
AP009048 Genomic DNA. Translation: BAA35738.2.
PIRiC64838. HQECL.
RefSeqiNP_415492.1. NC_000913.3.
YP_489244.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74058; AAC74058; b0973.
BAA35738; BAA35738; BAA35738.
GeneIDi12931049.
945580.
KEGGiecj:Y75_p0944.
eco:b0973.
PATRICi32117167. VBIEscCol129921_1007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34825 Genomic DNA. Translation: AAA23998.1 .
U00096 Genomic DNA. Translation: AAC74058.1 .
AP009048 Genomic DNA. Translation: BAA35738.2 .
PIRi C64838. HQECL.
RefSeqi NP_415492.1. NC_000913.3.
YP_489244.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3UQY X-ray 1.47 L/M 1-582 [» ]
3USC X-ray 2.00 L/M 1-582 [» ]
3USE X-ray 1.67 L/M 1-582 [» ]
4GD3 X-ray 3.30 J/K/L/M 1-582 [» ]
ProteinModelPortali P0ACD8.
SMRi P0ACD8. Positions 2-582.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36180N.
IntActi P0ACD8. 8 interactions.
STRINGi 511145.b0973.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74058 ; AAC74058 ; b0973 .
BAA35738 ; BAA35738 ; BAA35738 .
GeneIDi 12931049.
945580.
KEGGi ecj:Y75_p0944.
eco:b0973.
PATRICi 32117167. VBIEscCol129921_1007.

Organism-specific databases

EchoBASEi EB0464.
EcoGenei EG10469. hyaB.

Phylogenomic databases

eggNOGi COG0374.
HOGENOMi HOG000278799.
KOi K06281.
OMAi WVEENES.
OrthoDBi EOG6M9DSB.
PhylomeDBi P0ACD8.

Enzyme and pathway databases

BioCyci EcoCyc:HYAB-MONOMER.
ECOL316407:JW0955-MONOMER.
MetaCyc:HYAB-MONOMER.

Miscellaneous databases

PROi P0ACD8.

Gene expression databases

Genevestigatori P0ACD8.

Family and domain databases

Gene3Di 1.10.645.10. 1 hit.
InterProi IPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
IPR029014. NiFe_Hase-like.
[Graphical view ]
Pfami PF00374. NiFeSe_Hases. 1 hit.
[Graphical view ]
SUPFAMi SSF56762. SSF56762. 1 hit.
PROSITEi PS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
    Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
    J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-597.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiMBHL_ECOLI
AccessioniPrimary (citable) accession number: P0ACD8
Secondary accession number(s): P19927, P78056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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