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P0ACD8 (MBHL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydrogenase-1 large chain

Short name=HYD1
EC=1.12.99.6
Alternative name(s):
Membrane-bound hydrogenase 1 large subunit
NiFe hydrogenase
Gene names
Name:hyaB
Ordered Locus Names:b0973, JW0955
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

Catalytic activity

H2 + A = AH2.

Cofactor

Binds 1 nickel ion per subunit By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

hyaDP199303EBI-851493,EBI-552940

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Hydrogenase-1 large chain
PRO_0000199712

Sites

Metal binding761Nickel Potential
Metal binding791Nickel Potential
Metal binding5761Nickel Potential
Metal binding5791Nickel Potential

Experimental info

Sequence conflict521M → I in AAA23998. Ref.1
Sequence conflict691W → R in AAA23998. Ref.1

Secondary structure

.................................................................................................... 597
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ACD8 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 659DD7EDE16D6342

FASTA59766,253
        10         20         30         40         50         60 
MSTQYETQGY TINNAGRRLV VDPITRIEGH MRCEVNINDQ NVITNAVSCG TMFRGLEIIL 

        70         80         90        100        110        120 
QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP DNANIIRNIM LATLWCHDHL 

       130        140        150        160        170        180 
VHFYQLAGMD WIDVLDALKA DPRKTSELAQ SLSSWPKSSP GYFFDVQNRL KKFVEGGQLG 

       190        200        210        220        230        240 
IFRNGYWGHP QYKLPPEANL MGFAHYLEAL DFQREIVKIH AVFGGKNPHP NWIVGGMPCA 

       250        260        270        280        290        300 
INIDESGAVG AVNMERLNLV QSIITRTADF INNVMIPDAL AIGQFNKPWS EIGTGLSDKC 

       310        320        330        340        350        360 
VLSYGAFPDI ANDFGEKSLL MPGGAVINGD FNNVLPVDLV DPQQVQEFVD HAWYRYPNDQ 

       370        380        390        400        410        420 
VGRHPFDGIT DPWYNPGDVK GSDTNIQQLN EQERYSWIKA PRWRGNAMEV GPLARTLIAY 

       430        440        450        460        470        480 
HKGDAATVES VDRMMSALNL PLSGIQSTLG RILCRAHEAQ WAAGKLQYFF DKLMTNLKNG 

       490        500        510        520        530        540 
NLATASTEKW EPATWPTECR GVGFTEAPRG ALGHWAAIRD GKIDLYQCVV PTTWNASPRD 

       550        560        570        580        590 
PKGQIGAYEA ALMNTKMAIP EQPLEILRTL HSFDPCLACS THVLGDDGSE LISVQVR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-597.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34825 Genomic DNA. Translation: AAA23998.1.
U00096 Genomic DNA. Translation: AAC74058.1.
AP009048 Genomic DNA. Translation: BAA35738.2.
PIRHQECL. C64838.
RefSeqNP_415492.1. NC_000913.3.
YP_489244.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47L/M1-582[»]
3USCX-ray2.00L/M1-582[»]
3USEX-ray1.67L/M1-582[»]
4GD3X-ray3.30J/K/L/M1-582[»]
ProteinModelPortalP0ACD8.
SMRP0ACD8. Positions 2-582.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-36180N.
IntActP0ACD8. 8 interactions.
STRING511145.b0973.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74058; AAC74058; b0973.
BAA35738; BAA35738; BAA35738.
GeneID12931049.
945580.
KEGGecj:Y75_p0944.
eco:b0973.
PATRIC32117167. VBIEscCol129921_1007.

Organism-specific databases

EchoBASEEB0464.
EcoGeneEG10469. hyaB.

Phylogenomic databases

eggNOGCOG0374.
HOGENOMHOG000278799.
KOK06281.
OMAPIPRIEG.
OrthoDBEOG6M9DSB.
PhylomeDBP0ACD8.
ProtClustDBPRK10170.

Enzyme and pathway databases

BioCycEcoCyc:HYAB-MONOMER.
ECOL316407:JW0955-MONOMER.
MetaCyc:HYAB-MONOMER.

Gene expression databases

GenevestigatorP0ACD8.

Family and domain databases

InterProIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
[Graphical view]
PfamPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
PROSITEPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0ACD8.

Entry information

Entry nameMBHL_ECOLI
AccessionPrimary (citable) accession number: P0ACD8
Secondary accession number(s): P19927, P78056
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene