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P0ACD8

- MBHL_ECOLI

UniProt

P0ACD8 - MBHL_ECOLI

Protein

Hydrogenase-1 large chain

Gene

hyaB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

    Catalytic activityi

    H2 + A = AH2.

    Cofactori

    Binds 1 nickel ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761NickelSequence Analysis
    Metal bindingi79 – 791NickelSequence Analysis
    Metal bindingi576 – 5761NickelSequence Analysis
    Metal bindingi579 – 5791NickelSequence Analysis

    GO - Molecular functioni

    1. ferredoxin hydrogenase activity Source: InterPro
    2. hydrogenase (acceptor) activity Source: UniProtKB-EC
    3. nickel cation binding Source: InterPro
    4. protein binding Source: IntAct

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, Nickel

    Enzyme and pathway databases

    BioCyciEcoCyc:HYAB-MONOMER.
    ECOL316407:JW0955-MONOMER.
    MetaCyc:HYAB-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydrogenase-1 large chain (EC:1.12.99.6)
    Short name:
    HYD1
    Alternative name(s):
    Membrane-bound hydrogenase 1 large subunit
    NiFe hydrogenase
    Gene namesi
    Name:hyaB
    Ordered Locus Names:b0973, JW0955
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10469. hyaB.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 597597Hydrogenase-1 large chainPRO_0000199712Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACD8.

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    hyaDP199303EBI-851493,EBI-552940

    Protein-protein interaction databases

    DIPiDIP-36180N.
    IntActiP0ACD8. 8 interactions.
    STRINGi511145.b0973.

    Structurei

    Secondary structure

    1
    597
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi10 – 134
    Beta strandi16 – 216
    Beta strandi26 – 294
    Beta strandi31 – 377
    Beta strandi41 – 5010
    Helixi56 – 594
    Turni60 – 623
    Helixi65 – 673
    Helixi68 – 736
    Beta strandi77 – 793
    Helixi82 – 9514
    Helixi101 – 12525
    Helixi128 – 1303
    Helixi135 – 1395
    Helixi142 – 15211
    Helixi160 – 17516
    Helixi180 – 1823
    Helixi196 – 21621
    Helixi218 – 2247
    Beta strandi225 – 2295
    Beta strandi243 – 2453
    Helixi248 – 2503
    Helixi254 – 27320
    Helixi275 – 28511
    Helixi287 – 2915
    Helixi295 – 2984
    Beta strandi301 – 3033
    Beta strandi306 – 3105
    Turni316 – 3183
    Beta strandi319 – 3213
    Beta strandi324 – 3263
    Turni342 – 3443
    Beta strandi345 – 3484
    Beta strandi352 – 3543
    Helixi365 – 3673
    Beta strandi380 – 3823
    Beta strandi385 – 3884
    Beta strandi400 – 4034
    Helixi412 – 42110
    Helixi425 – 43713
    Helixi442 – 4454
    Helixi448 – 47831
    Helixi492 – 4943
    Beta strandi497 – 50711
    Beta strandi510 – 51910
    Beta strandi522 – 5298
    Helixi531 – 5355
    Helixi547 – 5526
    Beta strandi560 – 5623
    Helixi564 – 5729
    Helixi577 – 5815

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UQYX-ray1.47L/M1-582[»]
    3USCX-ray2.00L/M1-582[»]
    3USEX-ray1.67L/M1-582[»]
    4GD3X-ray3.30J/K/L/M1-582[»]
    ProteinModelPortaliP0ACD8.
    SMRiP0ACD8. Positions 2-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0374.
    HOGENOMiHOG000278799.
    KOiK06281.
    OMAiWVEENES.
    OrthoDBiEOG6M9DSB.
    PhylomeDBiP0ACD8.

    Family and domain databases

    Gene3Di1.10.645.10. 1 hit.
    InterProiIPR001501. Ni-dep_hyd_lsu.
    IPR018194. Ni-dep_hyd_lsu_Ni_BS.
    IPR029014. NiFe_Hase-like.
    [Graphical view]
    PfamiPF00374. NiFeSe_Hases. 1 hit.
    [Graphical view]
    SUPFAMiSSF56762. SSF56762. 1 hit.
    PROSITEiPS00507. NI_HGENASE_L_1. 1 hit.
    PS00508. NI_HGENASE_L_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACD8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTQYETQGY TINNAGRRLV VDPITRIEGH MRCEVNINDQ NVITNAVSCG    50
    TMFRGLEIIL QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP 100
    DNANIIRNIM LATLWCHDHL VHFYQLAGMD WIDVLDALKA DPRKTSELAQ 150
    SLSSWPKSSP GYFFDVQNRL KKFVEGGQLG IFRNGYWGHP QYKLPPEANL 200
    MGFAHYLEAL DFQREIVKIH AVFGGKNPHP NWIVGGMPCA INIDESGAVG 250
    AVNMERLNLV QSIITRTADF INNVMIPDAL AIGQFNKPWS EIGTGLSDKC 300
    VLSYGAFPDI ANDFGEKSLL MPGGAVINGD FNNVLPVDLV DPQQVQEFVD 350
    HAWYRYPNDQ VGRHPFDGIT DPWYNPGDVK GSDTNIQQLN EQERYSWIKA 400
    PRWRGNAMEV GPLARTLIAY HKGDAATVES VDRMMSALNL PLSGIQSTLG 450
    RILCRAHEAQ WAAGKLQYFF DKLMTNLKNG NLATASTEKW EPATWPTECR 500
    GVGFTEAPRG ALGHWAAIRD GKIDLYQCVV PTTWNASPRD PKGQIGAYEA 550
    ALMNTKMAIP EQPLEILRTL HSFDPCLACS THVLGDDGSE LISVQVR 597
    Length:597
    Mass (Da):66,253
    Last modified:November 22, 2005 - v1
    Checksum:i659DD7EDE16D6342
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521M → I in AAA23998. (PubMed:2180913)Curated
    Sequence conflicti69 – 691W → R in AAA23998. (PubMed:2180913)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34825 Genomic DNA. Translation: AAA23998.1.
    U00096 Genomic DNA. Translation: AAC74058.1.
    AP009048 Genomic DNA. Translation: BAA35738.2.
    PIRiC64838. HQECL.
    RefSeqiNP_415492.1. NC_000913.3.
    YP_489244.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74058; AAC74058; b0973.
    BAA35738; BAA35738; BAA35738.
    GeneIDi12931049.
    945580.
    KEGGiecj:Y75_p0944.
    eco:b0973.
    PATRICi32117167. VBIEscCol129921_1007.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34825 Genomic DNA. Translation: AAA23998.1 .
    U00096 Genomic DNA. Translation: AAC74058.1 .
    AP009048 Genomic DNA. Translation: BAA35738.2 .
    PIRi C64838. HQECL.
    RefSeqi NP_415492.1. NC_000913.3.
    YP_489244.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3UQY X-ray 1.47 L/M 1-582 [» ]
    3USC X-ray 2.00 L/M 1-582 [» ]
    3USE X-ray 1.67 L/M 1-582 [» ]
    4GD3 X-ray 3.30 J/K/L/M 1-582 [» ]
    ProteinModelPortali P0ACD8.
    SMRi P0ACD8. Positions 2-582.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36180N.
    IntActi P0ACD8. 8 interactions.
    STRINGi 511145.b0973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74058 ; AAC74058 ; b0973 .
    BAA35738 ; BAA35738 ; BAA35738 .
    GeneIDi 12931049.
    945580.
    KEGGi ecj:Y75_p0944.
    eco:b0973.
    PATRICi 32117167. VBIEscCol129921_1007.

    Organism-specific databases

    EchoBASEi EB0464.
    EcoGenei EG10469. hyaB.

    Phylogenomic databases

    eggNOGi COG0374.
    HOGENOMi HOG000278799.
    KOi K06281.
    OMAi WVEENES.
    OrthoDBi EOG6M9DSB.
    PhylomeDBi P0ACD8.

    Enzyme and pathway databases

    BioCyci EcoCyc:HYAB-MONOMER.
    ECOL316407:JW0955-MONOMER.
    MetaCyc:HYAB-MONOMER.

    Miscellaneous databases

    PROi P0ACD8.

    Gene expression databases

    Genevestigatori P0ACD8.

    Family and domain databases

    Gene3Di 1.10.645.10. 1 hit.
    InterProi IPR001501. Ni-dep_hyd_lsu.
    IPR018194. Ni-dep_hyd_lsu_Ni_BS.
    IPR029014. NiFe_Hase-like.
    [Graphical view ]
    Pfami PF00374. NiFeSe_Hases. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56762. SSF56762. 1 hit.
    PROSITEi PS00507. NI_HGENASE_L_1. 1 hit.
    PS00508. NI_HGENASE_L_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
      Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
      J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-597.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiMBHL_ECOLI
    AccessioniPrimary (citable) accession number: P0ACD8
    Secondary accession number(s): P19927, P78056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3