Reviewed,
UniProtKB/Swiss-Prot P0ACD8 (MBHL_ECOLI)
Last modified
June 16, 2009.
Version 32.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hydrogenase-1 large chain Short name=HYD1 EC=1.12.99.6 Alternative name(s): Membrane-bound hydrogenase 1 large subunit NiFe hydrogenase | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 597 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 nickel ion per subunit By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Ligand | Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activityInferred from electronic annotation. Source: EC nickel ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 597 | 597 | Hydrogenase-1 large chain | PRO_0000199712 | |||||
Sites | |||||||||
| Metal binding | 76 | 1 | Nickel Potential | ||||||
| Metal binding | 79 | 1 | Nickel Potential | ||||||
| Metal binding | 576 | 1 | Nickel Potential | ||||||
| Metal binding | 579 | 1 | Nickel Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 52 | 1 | M → I in AAA23998. Ref.1 | ||||||
| Sequence conflict | 69 | 1 | W → R in AAA23998. Ref.1 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| M34825 Genomic DNA. Translation: AAA23998.1. U00096 Genomic DNA. Translation: AAC74058.1. AP009048 Genomic DNA. Translation: BAA35738.2. | |
| PIR | HQECL. C64838. |
| RefSeq | AP_001602.1. NP_415492.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FRF based on UniProtKB P18188. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P0ACD8. 7 interactions. |
Genome annotation databases | |
| GeneID | 945580. |
| GenomeReviews | Gene locus JW0955 in contig AP009048_GR. Gene locus b0973 in contig U00096_GR. |
| KEGG | ecj:JW0955. eco:b0973. |
Organism-specific databases | |
| EchoBASE | EB0464. |
| EcoGene | EG10469. hyaB. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P0ACD8. |
| OMA | P0ACD8. CLACATH. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:HYAB-MON. MetaCyc:HYAB-MON. |
Family and domain databases | |
| InterPro | IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view] |
| Pfam | PF00374. NiFeSe_Hases. 1 hit. [Graphical view] |
| PROSITE | PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHL_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0ACD8 Secondary accession number(s): P19927, P78056 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


