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Protein

Iron-sulfur cluster assembly scaffold protein IscU

Gene

iscU

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (Probable).1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster assembly scaffold protein IscU
Alternative name(s):
Sulfur acceptor protein IscU
Gene namesi
Name:iscU
Synonyms:nifU
Ordered Locus Names:Z3796, ECs3395
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 12Missing : No longer binds IscS. 1 PublicationAdd BLAST12
Mutagenesisi1 – 6Missing : Partial loss of IscS binding. 1 Publication6
Mutagenesisi5E → L: Binds IscS. 1 Publication1
Mutagenesisi9D → R: Binds IscS. 1 Publication1
Mutagenesisi11Y → A: Binds IscS. 1 Publication1
Mutagenesisi98E → R: Binds IscS. 1 Publication1
Mutagenesisi103K → E: No longer binds IscS. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001661851 – 128Iron-sulfur cluster assembly scaffold protein IscUAdd BLAST128

Proteomic databases

PRIDEiP0ACD6

Interactioni

Subunit structurei

Forms a heterotetramer with IscS; each subunit of the IscS dimer contacts an IscU monomer. Certain pairs of proteins can bind simultaneously to IscS; IscS-IscU-CyaY and IscS-IscU-IscX complexes can be isolated in vitro, but others (IscS-IscU-TusA) cannot. IscU can displace TusA from IscS.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B95EBI-9011202,EBI-9011195

Protein-protein interaction databases

DIPiDIP-58575N
IntActiP0ACD6, 3 interactors
STRINGi155864.Z3796

Structurei

Secondary structure

1128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 12Combined sources8
Beta strandi15 – 18Combined sources4
Beta strandi25 – 33Combined sources9
Turni35 – 37Combined sources3
Beta strandi40 – 47Combined sources8
Beta strandi49 – 51Combined sources3
Beta strandi53 – 62Combined sources10
Helixi64 – 77Combined sources14
Helixi82 – 86Combined sources5
Helixi90 – 97Combined sources8
Helixi101 – 104Combined sources4
Helixi105 – 123Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LVLX-ray3.00A2-128[»]
ProteinModelPortaliP0ACD6
SMRiP0ACD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifU family.Curated

Phylogenomic databases

eggNOGiENOG4107XTC Bacteria
COG0822 LUCA
HOGENOMiHOG000069228
KOiK04488
OMAiSMVTEMV

Family and domain databases

CDDicd06664 IscU_like, 1 hit
InterProiView protein in InterPro
IPR011339 ISC_FeS_clus_asmbl_IscU
IPR002871 NIF_FeS_clus_asmbl_NifU_N
PANTHERiPTHR10093 PTHR10093, 1 hit
PfamiView protein in Pfam
PF01592 NifU_N, 1 hit
TIGRFAMsiTIGR01999 iscU, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE
60 70 80 90 100
GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP
110 120
PVKIHCSILA EDAIKAAIAD YKSKREAK
Length:128
Mass (Da):13,849
Last modified:November 22, 2005 - v1
Checksum:i47516021442B6535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57643.1
BA000007 Genomic DNA Translation: BAB36818.1
PIRiC91053
G85897
RefSeqiNP_311422.1, NC_002695.1
WP_000331707.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57643; AAG57643; Z3796
BAB36818; BAB36818; BAB36818
GeneIDi915163
KEGGiece:Z3796
ecs:ECs3395
PATRICifig|386585.9.peg.3547

Similar proteinsi

Entry informationi

Entry nameiISCU_ECO57
AccessioniPrimary (citable) accession number: P0ACD6
Secondary accession number(s): P77310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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