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Protein

Putative colanic acid biosynthesis acetyltransferase WcaF

Gene

wcaF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Pathwayi: slime polysaccharide biosynthesis

This protein is involved in the pathway slime polysaccharide biosynthesis, which is part of Slime biogenesis.
View all proteins of this organism that are known to be involved in the pathway slime polysaccharide biosynthesis and in Slime biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7099-MONOMER.
ECOL316407:JW2039-MONOMER.
UniPathwayiUPA00936.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative colanic acid biosynthesis acetyltransferase WcaF (EC:2.3.1.-)
Gene namesi
Name:wcaF
Ordered Locus Names:b2054, JW2039
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13574. wcaF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 182182Putative colanic acid biosynthesis acetyltransferase WcaFPRO_0000068736Add
BLAST

Proteomic databases

PaxDbiP0ACD2.
PRIDEiP0ACD2.

Interactioni

Protein-protein interaction databases

BioGridi4259689. 200 interactions.
IntActiP0ACD2. 1 interaction.
STRINGi511145.b2054.

Structurei

3D structure databases

ProteinModelPortaliP0ACD2.
SMRiP0ACD2. Positions 57-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108Z90. Bacteria.
COG0110. LUCA.
HOGENOMiHOG000049439.
InParanoidiP0ACD2.
KOiK03818.
OMAiCWGSPCR.
OrthoDBiEOG6P335K.
PhylomeDBiP0ACD2.

Family and domain databases

InterProiIPR024005. Colanic_acid_synth_WcaF.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR04008. WcaF. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDLSGFSVP KGFRGGNAIK VQLWWAVQAT IFAWSPQVLY RWRAFLLRLF
60 70 80 90 100
GAKIGKNVVI RPSVKITYPW KLTLGDYAWV GDDVNLYTLG EITIGAHSVI
110 120 130 140 150
SQKSYLCTGS HDHASQHFTI NATPIVIGEK CWLATDVFVA PGVTIGDGTV
160 170 180
VGARSSVFKS LPANVVCRGN PAVVIRERVE TE
Length:182
Mass (Da):19,962
Last modified:November 8, 2005 - v1
Checksum:i8AA5E672CA802442
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501F → L in AAC77841 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77841.1.
U00096 Genomic DNA. Translation: AAC75115.1.
AP009048 Genomic DNA. Translation: BAA15910.1.
PIRiE64971.
RefSeqiNP_416558.1. NC_000913.3.
WP_001153547.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75115; AAC75115; b2054.
BAA15910; BAA15910; BAA15910.
GeneIDi946578.
KEGGiecj:JW2039.
eco:b2054.
PATRICi32119441. VBIEscCol129921_2131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77841.1.
U00096 Genomic DNA. Translation: AAC75115.1.
AP009048 Genomic DNA. Translation: BAA15910.1.
PIRiE64971.
RefSeqiNP_416558.1. NC_000913.3.
WP_001153547.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACD2.
SMRiP0ACD2. Positions 57-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259689. 200 interactions.
IntActiP0ACD2. 1 interaction.
STRINGi511145.b2054.

Proteomic databases

PaxDbiP0ACD2.
PRIDEiP0ACD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75115; AAC75115; b2054.
BAA15910; BAA15910; BAA15910.
GeneIDi946578.
KEGGiecj:JW2039.
eco:b2054.
PATRICi32119441. VBIEscCol129921_2131.

Organism-specific databases

EchoBASEiEB3344.
EcoGeneiEG13574. wcaF.

Phylogenomic databases

eggNOGiENOG4108Z90. Bacteria.
COG0110. LUCA.
HOGENOMiHOG000049439.
InParanoidiP0ACD2.
KOiK03818.
OMAiCWGSPCR.
OrthoDBiEOG6P335K.
PhylomeDBiP0ACD2.

Enzyme and pathway databases

UniPathwayiUPA00936.
BioCyciEcoCyc:G7099-MONOMER.
ECOL316407:JW2039-MONOMER.

Miscellaneous databases

PROiP0ACD2.

Family and domain databases

InterProiIPR024005. Colanic_acid_synth_WcaF.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR04008. WcaF. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid."
    Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.
    J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Reeves P.R.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiWCAF_ECOLI
AccessioniPrimary (citable) accession number: P0ACD2
Secondary accession number(s): P71240, P76383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: January 20, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.