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Protein

Iron-sulfur cluster insertion protein ErpA

Gene

erpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably involved in the insertion of Fe-S clusters into apoproteins in vivo including IspG and/or IspH. Essential for growth under aerobic conditions and for anaerobic respiration but not for fermentation. In vitro it binds Fe-S clusters and transfers them to apo-IspG, which is involved in quinone biosynthesis among many other cell components. Experiments indicate that it is probably also involved in the insertion of other Fe-S clusters than IspG/IspH.

Cofactori

iron-sulfur cluster1 PublicationNote: Binds 1 iron-sulfur cluster per subunit. A study found 40-50% as [2Fe-2S] clusters, 15-25% as [4Fe-4S] clusters and the rest as paramagnetic iron.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfurCurated1
Metal bindingi106Iron-sulfurCurated1
Metal bindingi108Iron-sulfurCurated1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron ion binding Source: GO_Central
  • structural molecule activity Source: InterPro

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc
  • iron-sulfur cluster assembly Source: EcoCyc
  • protein maturation Source: EcoCyc
  • protein maturation by iron-sulfur cluster transfer Source: InterPro

Keywordsi

LigandIron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12332-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster insertion protein ErpA
Gene namesi
Name:erpA
Synonyms:yadR
Ordered Locus Names:b0156, JW0152
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12332 erpA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of quinones.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42C → S: Does not complement knockout. 1 Publication1
Mutagenesisi106C → S: Does not complement knockout. 1 Publication1
Mutagenesisi108C → S: Does not complement knockout. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769901 – 114Iron-sulfur cluster insertion protein ErpAAdd BLAST114

Proteomic databases

EPDiP0ACC3
PaxDbiP0ACC3
PRIDEiP0ACC3

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi4261657, 299 interactors
DIPiDIP-48196N
IntActiP0ACC3, 8 interactors
STRINGi316385.ECDH10B_0136

Structurei

3D structure databases

ProteinModelPortaliP0ACC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

eggNOGiENOG4108YX9 Bacteria
COG0316 LUCA
HOGENOMiHOG000228313
InParanoidiP0ACC3
KOiK15724
OMAiRTCGCGE
PhylomeDBiP0ACC3

Family and domain databases

Gene3Di2.60.300.12, 1 hit
HAMAPiMF_01380 Fe_S_insert_ErpA, 1 hit
InterProiView protein in InterPro
IPR000361 FeS_biogenesis
IPR016092 FeS_cluster_insertion
IPR017870 FeS_cluster_insertion_CS
IPR023063 FeS_cluster_insertion_RrpA
IPR035903 HesB-like_dom_sf
PfamiView protein in Pfam
PF01521 Fe-S_biosyn, 1 hit
SUPFAMiSSF89360 SSF89360, 1 hit
TIGRFAMsiTIGR00049 TIGR00049, 1 hit
PROSITEiView protein in PROSITE
PS01152 HESB, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDVALPLE FTDAAANKVK SLIADEDNPN LKLRVYITGG GCSGFQYGFT
60 70 80 90 100
FDDQVNEGDM TIEKQGVGLV VDPMSLQYLV GGSVDYTEGL EGSRFIVTNP
110
NAKSTCGCGS SFSI
Length:114
Mass (Da):12,100
Last modified:November 8, 2005 - v1
Checksum:i5A032FB5C3E0D04A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08586.1
U00096 Genomic DNA Translation: AAC73267.1
AP009048 Genomic DNA Translation: BAB96733.1
PIRiS45225
RefSeqiNP_414698.1, NC_000913.3
WP_001295564.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73267; AAC73267; b0156
BAB96733; BAB96733; BAB96733
GeneIDi944857
KEGGiecj:JW0152
eco:b0156
PATRICifig|1411691.4.peg.2124

Similar proteinsi

Entry informationi

Entry nameiERPA_ECOLI
AccessioniPrimary (citable) accession number: P0ACC3
Secondary accession number(s): P37026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
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