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Protein

Iron-sulfur cluster insertion protein ErpA

Gene

erpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in the insertion of Fe-S clusters into apoproteins in vivo including IspG and/or IspH. Essential for growth under aerobic conditions and for anaerobic respiration but not for fermentation. In vitro it binds Fe-S clusters and transfers them to apo-IspG, which is involved in quinone biosynthesis among many other cell components. Experiments indicate that it is probably also involved in the insertion of other Fe-S clusters than IspG/IspH.

Cofactori

iron-sulfur cluster1 PublicationNote: Binds 1 iron-sulfur cluster per subunit. A study found 40-50% as [2Fe-2S] clusters, 15-25% as [4Fe-4S] clusters and the rest as paramagnetic iron.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Iron-sulfurCurated
Metal bindingi106 – 1061Iron-sulfurCurated
Metal bindingi108 – 1081Iron-sulfurCurated

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ferrous iron binding Source: GO_Central
  • structural molecule activity Source: InterPro

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc
  • iron-sulfur cluster assembly Source: EcoCyc
  • protein maturation Source: EcoCyc
  • protein maturation by iron-sulfur cluster transfer Source: GO_Central
  • sulfur compound metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12332-MONOMER.
ECOL316407:JW0152-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster insertion protein ErpA
Gene namesi
Name:erpA
Synonyms:yadR
Ordered Locus Names:b0156, JW0152
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12332. erpA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of quinones.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421C → S: Does not complement knockout. 1 Publication
Mutagenesisi106 – 1061C → S: Does not complement knockout. 1 Publication
Mutagenesisi108 – 1081C → S: Does not complement knockout. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 114114Iron-sulfur cluster insertion protein ErpAPRO_0000076990Add
BLAST

Proteomic databases

EPDiP0ACC3.
PaxDbiP0ACC3.
PRIDEiP0ACC3.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi4261657. 298 interactions.
DIPiDIP-48196N.
IntActiP0ACC3. 8 interactions.
STRINGi511145.b0156.

Structurei

3D structure databases

ProteinModelPortaliP0ACC3.
SMRiP0ACC3. Positions 1-114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

eggNOGiENOG4108YX9. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228313.
InParanoidiP0ACC3.
KOiK15724.
OMAiFQYKFDV.
PhylomeDBiP0ACC3.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01380. Fe_S_insert_ErpA. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR023063. FeS_cluster_insertion_RrpA.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDVALPLE FTDAAANKVK SLIADEDNPN LKLRVYITGG GCSGFQYGFT
60 70 80 90 100
FDDQVNEGDM TIEKQGVGLV VDPMSLQYLV GGSVDYTEGL EGSRFIVTNP
110
NAKSTCGCGS SFSI
Length:114
Mass (Da):12,100
Last modified:November 8, 2005 - v1
Checksum:i5A032FB5C3E0D04A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08586.1.
U00096 Genomic DNA. Translation: AAC73267.1.
AP009048 Genomic DNA. Translation: BAB96733.1.
PIRiS45225.
RefSeqiNP_414698.1. NC_000913.3.
WP_001295564.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73267; AAC73267; b0156.
BAB96733; BAB96733; BAB96733.
GeneIDi944857.
KEGGiecj:JW0152.
eco:b0156.
PATRICi32115421. VBIEscCol129921_0162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08586.1.
U00096 Genomic DNA. Translation: AAC73267.1.
AP009048 Genomic DNA. Translation: BAB96733.1.
PIRiS45225.
RefSeqiNP_414698.1. NC_000913.3.
WP_001295564.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACC3.
SMRiP0ACC3. Positions 1-114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261657. 298 interactions.
DIPiDIP-48196N.
IntActiP0ACC3. 8 interactions.
STRINGi511145.b0156.

Proteomic databases

EPDiP0ACC3.
PaxDbiP0ACC3.
PRIDEiP0ACC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73267; AAC73267; b0156.
BAB96733; BAB96733; BAB96733.
GeneIDi944857.
KEGGiecj:JW0152.
eco:b0156.
PATRICi32115421. VBIEscCol129921_0162.

Organism-specific databases

EchoBASEiEB2236.
EcoGeneiEG12332. erpA.

Phylogenomic databases

eggNOGiENOG4108YX9. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228313.
InParanoidiP0ACC3.
KOiK15724.
OMAiFQYKFDV.
PhylomeDBiP0ACC3.

Enzyme and pathway databases

BioCyciEcoCyc:EG12332-MONOMER.
ECOL316407:JW0152-MONOMER.

Miscellaneous databases

PROiP0ACC3.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01380. Fe_S_insert_ErpA. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR023063. FeS_cluster_insertion_RrpA.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERPA_ECOLI
AccessioniPrimary (citable) accession number: P0ACC3
Secondary accession number(s): P37026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.