P0ACC3 (ERPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iron-sulfur cluster insertion protein ErpA

Gene names

Name:	erpA
Synonyms:	yadR
Ordered Locus Names:	b0156, JW0152

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the insertion of Fe-S clusters into apoproteins in vivo including IspG and/or IspH. Essential for growth under aerobic conditions and for anaerobic respiration but not for fermentation. In vitro it binds Fe-S clusters and transfers them to apo-IspG, which is involved in quinone biosynthesis among many other cell components. Experiments indicate that it is probably also involved in the insertion of other Fe-S clusters than IspG/IspH. HAMAP-Rule MF_01380
Cofactor	Binds 1 iron-sulfur cluster per subunit. A study found 40-50% as 2Fe-2S clusters, 15-25% as 4Fe-4S clusters and the rest as paramagnetic iron.
Subunit structure	Homodimer Probable.
Disruption phenotype	Reduced amounts of quinones. Ref.5
Sequence similarities	Belongs to the HesB/IscA family.

Ontologies

Keywords
Ligand	Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype Ref.5. Source: EcoCyc anaerobic respiration Inferred from mutant phenotype Ref.5. Source: EcoCyc iron-sulfur cluster assembly Inferred from direct assay Ref.5. Source: EcoCyc protein maturation Inferred from direct assay Ref.5. Source: EcoCyc
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from direct assay Ref.5. Source: EcoCyc 4 iron, 4 sulfur cluster binding Inferred from direct assay Ref.5. Source: EcoCyc iron ion binding Inferred from electronic annotation. Source: HAMAP structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 114	114	Iron-sulfur cluster insertion protein ErpA HAMAP-Rule MF_01380		PRO_0000076990
Sites
	Metal binding	42	1	Iron-sulfur Probable
	Metal binding	106	1	Iron-sulfur Probable
	Metal binding	108	1	Iron-sulfur Probable
Experimental info
	Mutagenesis	42	1	C → S: Does not complement knockout. Ref.5
	Mutagenesis	106	1	C → S: Does not complement knockout. Ref.5
	Mutagenesis	108	1	C → S: Does not complement knockout. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P0ACC3 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5A032FB5C3E0D04A	FASTA	114	12,100
	10 20 30 40 50 60 MSDDVALPLE FTDAAANKVK SLIADEDNPN LKLRVYITGG GCSGFQYGFT FDDQVNEGDM 70 80 90 100 110 TIEKQGVGLV VDPMSLQYLV GGSVDYTEGL EGSRFIVTNP NAKSTCGCGS SFSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coli." Loiseau L., Gerez C., Bekker M., Ollagnier-de Choudens S., Py B., Sanakis Y., Teixeira de Mattos J., Fontecave M., Barras F. Proc. Natl. Acad. Sci. U.S.A. 104:13626-13631(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE, COFACTOR, MUTAGENESIS OF CYS-42; CYS-106 AND CYS-108. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70214 Genomic DNA. Translation: AAB08586.1. U00096 Genomic DNA. Translation: AAC73267.1. AP009048 Genomic DNA. Translation: BAB96733.1.
PIR	S45225.
RefSeq	NP_414698.1. NC_000913.2. YP_488459.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACC3.
SMR	P0ACC3. Positions 1-114.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48196N.
IntAct	P0ACC3. 8 interactions.
STRING	511145.b0156.
Proteomic databases
PaxDb	P0ACC3.
PRIDE	P0ACC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73267; AAC73267; b0156. BAB96733; BAB96733; BAB96733.
GeneID	12932663. 944857.
KEGG	ecj:Y75_p0153. eco:b0156.
PATRIC	32115421. VBIEscCol129921_0162.
Organism-specific databases
EchoBASE	EB2236.
EcoGene	EG12332. erpA.
Phylogenomic databases
eggNOG	COG0316.
HOGENOM	HOG000228313.
KO	K15724.
OMA	SSTCGCG.
ProtClustDB	PRK13623.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12332-MONOMER. ECOL316407:JW0152-MONOMER.
Gene expression databases
Genevestigator	P0ACC3.
Family and domain databases
Gene3D	2.60.300.12. 1 hit.
HAMAP	MF_01380. Fe_S_insert_ErpA.
InterPro	IPR000361. FeS_biogenesis. IPR016092. FeS_cluster_insertion. IPR017870. FeS_cluster_insertion_CS. IPR023063. FeS_cluster_insertion_RrpA. [Graphical view]
Pfam	PF01521. Fe-S_biosyn. 1 hit. [Graphical view]
SUPFAM	SSF89360. HesB_yadR_yfhF-like. 1 hit.
TIGRFAMs	TIGR00049. TIGR00049. 1 hit.
PROSITE	PS01152. HESB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERPA_ECOLI

Accession

Primary (citable) accession number: P0ACC3
Secondary accession number(s): P37026

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program