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Protein

Iron-sulfur cluster insertion protein ErpA

Gene

erpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in the insertion of Fe-S clusters into apoproteins in vivo including IspG and/or IspH. Essential for growth under aerobic conditions and for anaerobic respiration but not for fermentation. In vitro it binds Fe-S clusters and transfers them to apo-IspG, which is involved in quinone biosynthesis among many other cell components. Experiments indicate that it is probably also involved in the insertion of other Fe-S clusters than IspG/IspH.

Cofactori

iron-sulfur cluster1 PublicationNote: Binds 1 iron-sulfur cluster per subunit. A study found 40-50% as [2Fe-2S] clusters, 15-25% as [4Fe-4S] clusters and the rest as paramagnetic iron.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Iron-sulfurCurated
Metal bindingi106 – 1061Iron-sulfurCurated
Metal bindingi108 – 1081Iron-sulfurCurated

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ferrous iron binding Source: GO_Central
  • structural molecule activity Source: InterPro

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc
  • iron-sulfur cluster assembly Source: EcoCyc
  • protein maturation Source: EcoCyc
  • protein maturation by iron-sulfur cluster transfer Source: GO_Central
  • sulfur compound metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12332-MONOMER.
ECOL316407:JW0152-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster insertion protein ErpA
Gene namesi
Name:erpA
Synonyms:yadR
Ordered Locus Names:b0156, JW0152
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12332. erpA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of quinones.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421C → S: Does not complement knockout. 1 Publication
Mutagenesisi106 – 1061C → S: Does not complement knockout. 1 Publication
Mutagenesisi108 – 1081C → S: Does not complement knockout. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 114114Iron-sulfur cluster insertion protein ErpAPRO_0000076990Add
BLAST

Proteomic databases

EPDiP0ACC3.
PaxDbiP0ACC3.
PRIDEiP0ACC3.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi4261657. 298 interactions.
DIPiDIP-48196N.
IntActiP0ACC3. 8 interactions.
STRINGi511145.b0156.

Structurei

3D structure databases

ProteinModelPortaliP0ACC3.
SMRiP0ACC3. Positions 1-114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

eggNOGiENOG4108YX9. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228313.
InParanoidiP0ACC3.
KOiK15724.
OMAiFDENVND.
OrthoDBiEOG6VXF8J.
PhylomeDBiP0ACC3.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01380. Fe_S_insert_ErpA.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR023063. FeS_cluster_insertion_RrpA.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDVALPLE FTDAAANKVK SLIADEDNPN LKLRVYITGG GCSGFQYGFT
60 70 80 90 100
FDDQVNEGDM TIEKQGVGLV VDPMSLQYLV GGSVDYTEGL EGSRFIVTNP
110
NAKSTCGCGS SFSI
Length:114
Mass (Da):12,100
Last modified:November 8, 2005 - v1
Checksum:i5A032FB5C3E0D04A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08586.1.
U00096 Genomic DNA. Translation: AAC73267.1.
AP009048 Genomic DNA. Translation: BAB96733.1.
PIRiS45225.
RefSeqiNP_414698.1. NC_000913.3.
WP_001295564.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73267; AAC73267; b0156.
BAB96733; BAB96733; BAB96733.
GeneIDi944857.
KEGGiecj:JW0152.
eco:b0156.
PATRICi32115421. VBIEscCol129921_0162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08586.1.
U00096 Genomic DNA. Translation: AAC73267.1.
AP009048 Genomic DNA. Translation: BAB96733.1.
PIRiS45225.
RefSeqiNP_414698.1. NC_000913.3.
WP_001295564.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACC3.
SMRiP0ACC3. Positions 1-114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261657. 298 interactions.
DIPiDIP-48196N.
IntActiP0ACC3. 8 interactions.
STRINGi511145.b0156.

Proteomic databases

EPDiP0ACC3.
PaxDbiP0ACC3.
PRIDEiP0ACC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73267; AAC73267; b0156.
BAB96733; BAB96733; BAB96733.
GeneIDi944857.
KEGGiecj:JW0152.
eco:b0156.
PATRICi32115421. VBIEscCol129921_0162.

Organism-specific databases

EchoBASEiEB2236.
EcoGeneiEG12332. erpA.

Phylogenomic databases

eggNOGiENOG4108YX9. Bacteria.
COG0316. LUCA.
HOGENOMiHOG000228313.
InParanoidiP0ACC3.
KOiK15724.
OMAiFDENVND.
OrthoDBiEOG6VXF8J.
PhylomeDBiP0ACC3.

Enzyme and pathway databases

BioCyciEcoCyc:EG12332-MONOMER.
ECOL316407:JW0152-MONOMER.

Miscellaneous databases

PROiP0ACC3.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
HAMAPiMF_01380. Fe_S_insert_ErpA.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
IPR023063. FeS_cluster_insertion_RrpA.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coli."
    Loiseau L., Gerez C., Bekker M., Ollagnier-de Choudens S., Py B., Sanakis Y., Teixeira de Mattos J., Fontecave M., Barras F.
    Proc. Natl. Acad. Sci. U.S.A. 104:13626-13631(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE, COFACTOR, MUTAGENESIS OF CYS-42; CYS-106 AND CYS-108.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiERPA_ECOLI
AccessioniPrimary (citable) accession number: P0ACC3
Secondary accession number(s): P37026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 16, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.