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Protein

Release factor glutamine methyltransferase

Gene

prmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140S-adenosyl-L-methionine2 Publications1
Binding sitei168S-adenosyl-L-methionine2 Publications1
Binding sitei183S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein-glutamine N-methyltransferase activity Source: UniProtKB
  • protein methyltransferase activity Source: UniProtKB
  • S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB

GO - Biological processi

  • peptidyl-glutamine methylation Source: UniProtKB
  • protein methylation Source: UniProtKB
  • regulation of gene expression Source: UniProtKB
  • translational termination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12424-MONOMER.
ECOL316407:JW1203-MONOMER.
MetaCyc:EG12424-MONOMER.
BRENDAi2.1.1.297. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.2972 Publications)
Short name:
RF MTaseUniRule annotation
Alternative name(s):
M.EcoKHemKP
N5-glutamine methyltransferase PrmCUniRule annotation
Protein release factor methylation C
Protein-(glutamine-N5) MTase PrmCUniRule annotation
Protein-glutamine N-methyltransferase PrmCUniRule annotation
Gene namesi
Name:prmC
Synonyms:hemK
Ordered Locus Names:b1212, JW1203
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12424. prmC.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene suffer severe growth defects, but also show a global shift in gene expression to anaerobic respiration. Also show defects in translational termination via an enhanced rate of read-through of nonsense codons and induction of transfer-mRNA-mediated tagging of proteins within the cell.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36R → A: 17% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi40L → A: 60% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi41A → R: 7% of wild-type RF1 methylation activity. Strongly reduced affinity for S-adenosyl-L-methionine. 1 Publication1
Mutagenesisi42F → A: 40% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → A: 62% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → R: 33% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi183N → A: Less than 2% of wild-type RF1 methylation activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001571561 – 277Release factor glutamine methyltransferaseAdd BLAST277

Proteomic databases

PaxDbiP0ACC1.
PRIDEiP0ACC1.

Interactioni

Subunit structurei

Interacts with PrfA and PrfB.2 Publications

Protein-protein interaction databases

IntActiP0ACC1. 1 interactor.
STRINGi511145.b1212.

Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Turni13 – 16Combined sources4
Helixi20 – 32Combined sources13
Helixi36 – 41Combined sources6
Turni42 – 44Combined sources3
Helixi49 – 63Combined sources15
Helixi68 – 71Combined sources4
Beta strandi74 – 77Combined sources4
Beta strandi80 – 83Combined sources4
Helixi95 – 105Combined sources11
Beta strandi112 – 116Combined sources5
Helixi122 – 130Combined sources9
Beta strandi134 – 139Combined sources6
Helixi143 – 156Combined sources14
Beta strandi160 – 164Combined sources5
Helixi170 – 172Combined sources3
Beta strandi177 – 182Combined sources6
Helixi192 – 195Combined sources4
Helixi198 – 200Combined sources3
Turni205 – 207Combined sources3
Helixi210 – 213Combined sources4
Helixi215 – 224Combined sources10
Helixi225 – 227Combined sources3
Beta strandi228 – 237Combined sources10
Helixi240 – 242Combined sources3
Helixi243 – 252Combined sources10
Beta strandi260 – 262Combined sources3
Beta strandi266 – 274Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T43X-ray3.20A1-277[»]
2B3TX-ray3.10A1-276[»]
ProteinModelPortaliP0ACC1.
SMRiP0ACC1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACC1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 121S-adenosyl-L-methionine bindingUniRule annotation1 Publication5
Regioni183 – 186Substrate binding1 Publication4

Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076274.
InParanoidiP0ACC1.
KOiK02493.
OMAiHGWTQGE.
PhylomeDBiP0ACC1.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR025714. Methyltranfer_dom.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ACC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYQHWLREA ISQLQASESP RRDAEILLEH VTGRGRTFIL AFGETQLTDE
60 70 80 90 100
QCQQLDALLT RRRDGEPIAH LTGVREFWSL PLFVSPATLI PRPDTECLVE
110 120 130 140 150
QALARLPEQP CRILDLGTGT GAIALALASE RPDCEIIAVD RMPDAVSLAQ
160 170 180 190 200
RNAQHLAIKN IHILQSDWFS ALAGQQFAMI VSNPPYIDEQ DPHLQQGDVR
210 220 230 240 250
FEPLTALVAA DSGMADIVHI IEQSRNALVS GGFLLLEHGW QQGEAVRQAF
260 270
ILAGYHDVET CRDYGDNERV TLGRYYQ
Length:277
Mass (Da):30,975
Last modified:November 8, 2005 - v1
Checksum:i73859DF7E983B9AA
GO

Sequence cautioni

The sequence BAA05915 differs from that shown. Reason: Frameshift at position 204.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28567 Genomic DNA. Translation: BAA05915.1. Frameshift.
U18555 Genomic DNA. Translation: AAC43438.1.
AB078778 Genomic DNA. Translation: BAB84109.1.
U00096 Genomic DNA. Translation: AAC74296.1.
AP009048 Genomic DNA. Translation: BAA36070.1.
PIRiI83570.
RefSeqiNP_415730.1. NC_000913.3.
WP_000456467.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74296; AAC74296; b1212.
BAA36070; BAA36070; BAA36070.
GeneIDi945779.
KEGGiecj:JW1203.
eco:b1212.
PATRICi32117676. VBIEscCol129921_1260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28567 Genomic DNA. Translation: BAA05915.1. Frameshift.
U18555 Genomic DNA. Translation: AAC43438.1.
AB078778 Genomic DNA. Translation: BAB84109.1.
U00096 Genomic DNA. Translation: AAC74296.1.
AP009048 Genomic DNA. Translation: BAA36070.1.
PIRiI83570.
RefSeqiNP_415730.1. NC_000913.3.
WP_000456467.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T43X-ray3.20A1-277[»]
2B3TX-ray3.10A1-276[»]
ProteinModelPortaliP0ACC1.
SMRiP0ACC1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0ACC1. 1 interactor.
STRINGi511145.b1212.

Proteomic databases

PaxDbiP0ACC1.
PRIDEiP0ACC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74296; AAC74296; b1212.
BAA36070; BAA36070; BAA36070.
GeneIDi945779.
KEGGiecj:JW1203.
eco:b1212.
PATRICi32117676. VBIEscCol129921_1260.

Organism-specific databases

EchoBASEiEB2323.
EcoGeneiEG12424. prmC.

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076274.
InParanoidiP0ACC1.
KOiK02493.
OMAiHGWTQGE.
PhylomeDBiP0ACC1.

Enzyme and pathway databases

BioCyciEcoCyc:EG12424-MONOMER.
ECOL316407:JW1203-MONOMER.
MetaCyc:EG12424-MONOMER.
BRENDAi2.1.1.297. 2026.

Miscellaneous databases

EvolutionaryTraceiP0ACC1.
PROiP0ACC1.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR025714. Methyltranfer_dom.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRMC_ECOLI
AccessioniPrimary (citable) accession number: P0ACC1
Secondary accession number(s): P37186, Q46754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in the oxidation of protoporphyrinogen into protoporphyrin IX.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.