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Protein

Release factor glutamine methyltransferase

Gene

prmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.3 Publications

Caution

Was originally thought to be involved in the oxidation of protoporphyrinogen into protoporphyrin IX.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140S-adenosyl-L-methionine2 Publications1
Binding sitei168S-adenosyl-L-methionine2 Publications1
Binding sitei183S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein-(glutamine-N5) methyltransferase activity Source: UniProtKB-EC
  • protein-glutamine N-methyltransferase activity Source: UniProtKB
  • protein methyltransferase activity Source: UniProtKB
  • S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB

GO - Biological processi

  • peptidyl-glutamine methylation Source: UniProtKB
  • protein methylation Source: UniProtKB
  • regulation of gene expression Source: UniProtKB
  • translational termination Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12424-MONOMER
MetaCyc:EG12424-MONOMER
BRENDAi2.1.1.297 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.2972 Publications)
Short name:
RF MTaseUniRule annotation
Alternative name(s):
M.EcoKHemKP
N5-glutamine methyltransferase PrmCUniRule annotation
Protein release factor methylation C
Protein-(glutamine-N5) MTase PrmCUniRule annotation
Protein-glutamine N-methyltransferase PrmCUniRule annotation
Gene namesi
Name:prmC
Synonyms:hemK
Ordered Locus Names:b1212, JW1203
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12424 prmC

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene suffer severe growth defects, but also show a global shift in gene expression to anaerobic respiration. Also show defects in translational termination via an enhanced rate of read-through of nonsense codons and induction of transfer-mRNA-mediated tagging of proteins within the cell.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36R → A: 17% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi40L → A: 60% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi41A → R: 7% of wild-type RF1 methylation activity. Strongly reduced affinity for S-adenosyl-L-methionine. 1 Publication1
Mutagenesisi42F → A: 40% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → A: 62% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → R: 33% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi183N → A: Less than 2% of wild-type RF1 methylation activity. 1 Publication1

Chemistry databases

DrugBankiDB01752 S-Adenosyl-L-Homocysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001571561 – 277Release factor glutamine methyltransferaseAdd BLAST277

Proteomic databases

PaxDbiP0ACC1
PRIDEiP0ACC1

Interactioni

Subunit structurei

Interacts with PrfA and PrfB.2 Publications

Protein-protein interaction databases

BioGridi4260113, 35 interactors
IntActiP0ACC1, 1 interactor
STRINGi316385.ECDH10B_1265

Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Turni13 – 16Combined sources4
Helixi20 – 32Combined sources13
Helixi36 – 41Combined sources6
Turni42 – 44Combined sources3
Helixi49 – 63Combined sources15
Helixi68 – 71Combined sources4
Beta strandi74 – 77Combined sources4
Beta strandi80 – 83Combined sources4
Helixi95 – 105Combined sources11
Beta strandi112 – 116Combined sources5
Helixi122 – 130Combined sources9
Beta strandi134 – 139Combined sources6
Helixi143 – 156Combined sources14
Beta strandi160 – 164Combined sources5
Helixi170 – 172Combined sources3
Beta strandi177 – 182Combined sources6
Helixi192 – 195Combined sources4
Helixi198 – 200Combined sources3
Turni205 – 207Combined sources3
Helixi210 – 213Combined sources4
Helixi215 – 224Combined sources10
Helixi225 – 227Combined sources3
Beta strandi228 – 237Combined sources10
Helixi240 – 242Combined sources3
Helixi243 – 252Combined sources10
Beta strandi260 – 262Combined sources3
Beta strandi266 – 274Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T43X-ray3.20A1-277[»]
2B3TX-ray3.10A1-276[»]
ProteinModelPortaliP0ACC1
SMRiP0ACC1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACC1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 121S-adenosyl-L-methionine bindingUniRule annotation1 Publication5
Regioni183 – 186Substrate binding1 Publication4

Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EQY Bacteria
COG2890 LUCA
HOGENOMiHOG000076274
InParanoidiP0ACC1
KOiK02493
OMAiMLVSNPP
PhylomeDBiP0ACC1

Family and domain databases

HAMAPiMF_02126 RF_methyltr_PrmC, 1 hit
InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR025714 Methyltranfer_dom
IPR004556 Modification_methylase_HemK
IPR019874 Release_fac_Glu-N5_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF13847 Methyltransf_31, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00536 hemK_fam, 1 hit
TIGR03534 RF_mod_PrmC, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYQHWLREA ISQLQASESP RRDAEILLEH VTGRGRTFIL AFGETQLTDE
60 70 80 90 100
QCQQLDALLT RRRDGEPIAH LTGVREFWSL PLFVSPATLI PRPDTECLVE
110 120 130 140 150
QALARLPEQP CRILDLGTGT GAIALALASE RPDCEIIAVD RMPDAVSLAQ
160 170 180 190 200
RNAQHLAIKN IHILQSDWFS ALAGQQFAMI VSNPPYIDEQ DPHLQQGDVR
210 220 230 240 250
FEPLTALVAA DSGMADIVHI IEQSRNALVS GGFLLLEHGW QQGEAVRQAF
260 270
ILAGYHDVET CRDYGDNERV TLGRYYQ
Length:277
Mass (Da):30,975
Last modified:November 8, 2005 - v1
Checksum:i73859DF7E983B9AA
GO

Sequence cautioni

The sequence BAA05915 differs from that shown. Reason: Frameshift at position 204.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28567 Genomic DNA Translation: BAA05915.1 Frameshift.
U18555 Genomic DNA Translation: AAC43438.1
AB078778 Genomic DNA Translation: BAB84109.1
U00096 Genomic DNA Translation: AAC74296.1
AP009048 Genomic DNA Translation: BAA36070.1
PIRiI83570
RefSeqiNP_415730.1, NC_000913.3
WP_000456467.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74296; AAC74296; b1212
BAA36070; BAA36070; BAA36070
GeneIDi945779
KEGGiecj:JW1203
eco:b1212
PATRICifig|1411691.4.peg.1072

Similar proteinsi

Entry informationi

Entry nameiPRMC_ECOLI
AccessioniPrimary (citable) accession number: P0ACC1
Secondary accession number(s): P37186, Q46754
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health