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Protein

Protoporphyrinogen IX dehydrogenase [menaquinone]

Gene

hemG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX using menaquinone as electron acceptor.

Catalytic activityi

Protoporphyrinogen IX + 3 menaquinone = protoporphyrin IX + 3 menaquinol.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen IX dehydrogenase [menaquinone] (hemG)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • electron carrier activity Source: InterPro
  • FMN binding Source: EcoCyc
  • menaquinone-dependent protoporphyrinogen oxidase activity Source: EcoCyc
  • oxygen-dependent protoporphyrinogen oxidase activity Source: EcoCyc

GO - Biological processi

  • heme biosynthetic process Source: EcoCyc
  • porphyrin-containing compound biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:PROTOPORGENOXI-MONOMER.
ECOL316407:JW3827-MONOMER.
MetaCyc:PROTOPORGENOXI-MONOMER.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen IX dehydrogenase [menaquinone] (EC:1.3.5.3)
Gene namesi
Name:hemG
Synonyms:yihB
Ordered Locus Names:b3850, JW3827
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11485. hemG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Protoporphyrinogen IX dehydrogenase [menaquinone]PRO_0000135259Add
BLAST

Proteomic databases

PaxDbiP0ACB4.

Interactioni

Subunit structurei

Belongs to a multi-protein complex.

Protein-protein interaction databases

DIPiDIP-35890N.
IntActiP0ACB4. 18 interactions.
STRINGi511145.b3850.

Structurei

3D structure databases

ProteinModelPortaliP0ACB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 172170Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108RR8. Bacteria.
COG4635. LUCA.
HOGENOMiHOG000251295.
InParanoidiP0ACB4.
KOiK00230.
OMAiKGTLECD.
PhylomeDBiP0ACB4.

Family and domain databases

Gene3Di3.40.50.360. 2 hits.
InterProiIPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR026816. Flavodoxin_dom.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF12724. Flavodoxin_5. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLILFSTR DGQTREIASY LASELKELGI QADVANVHRI EEPQWENYDR
60 70 80 90 100
VVIGASIRYG HYHSAFQEFV KKHATRLNSM PSAFYSVNLV ARKPEKRTPQ
110 120 130 140 150
TNSYARKFLM NSQWRPDRCA VIAGALRYPR YRWYDRFMIK LIMKMSGGET
160 170 180
DTRKEVVYTD WEQVANFARE IAHLTDKPTL K
Length:181
Mass (Da):21,226
Last modified:November 8, 2005 - v1
Checksum:iBCCB5AE594010026
GO

Sequence cautioni

The sequence X54687 differs from that shown. Reason: Frameshift at position 159. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54687 Genomic DNA. No translation available.
X68660 Genomic DNA. Translation: CAA48626.1.
M87049 Genomic DNA. Translation: AAA67647.1.
U00096 Genomic DNA. Translation: AAC76853.1.
AP009048 Genomic DNA. Translation: BAE77453.1.
PIRiJC2513.
RefSeqiNP_418292.1. NC_000913.3.
WP_000853959.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76853; AAC76853; b3850.
BAE77453; BAE77453; BAE77453.
GeneIDi948331.
KEGGiecj:JW3827.
eco:b3850.
PATRICi32123197. VBIEscCol129921_3964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54687 Genomic DNA. No translation available.
X68660 Genomic DNA. Translation: CAA48626.1.
M87049 Genomic DNA. Translation: AAA67647.1.
U00096 Genomic DNA. Translation: AAC76853.1.
AP009048 Genomic DNA. Translation: BAE77453.1.
PIRiJC2513.
RefSeqiNP_418292.1. NC_000913.3.
WP_000853959.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35890N.
IntActiP0ACB4. 18 interactions.
STRINGi511145.b3850.

Proteomic databases

PaxDbiP0ACB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76853; AAC76853; b3850.
BAE77453; BAE77453; BAE77453.
GeneIDi948331.
KEGGiecj:JW3827.
eco:b3850.
PATRICi32123197. VBIEscCol129921_3964.

Organism-specific databases

EchoBASEiEB1448.
EcoGeneiEG11485. hemG.

Phylogenomic databases

eggNOGiENOG4108RR8. Bacteria.
COG4635. LUCA.
HOGENOMiHOG000251295.
InParanoidiP0ACB4.
KOiK00230.
OMAiKGTLECD.
PhylomeDBiP0ACB4.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
BioCyciEcoCyc:PROTOPORGENOXI-MONOMER.
ECOL316407:JW3827-MONOMER.
MetaCyc:PROTOPORGENOXI-MONOMER.

Miscellaneous databases

PROiP0ACB4.

Family and domain databases

Gene3Di3.40.50.360. 2 hits.
InterProiIPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR026816. Flavodoxin_dom.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF12724. Flavodoxin_5. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEMG_ECOLI
AccessioniPrimary (citable) accession number: P0ACB4
Secondary accession number(s): P27863, Q2M8F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.