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Protein

Protoporphyrinogen IX dehydrogenase [menaquinone]

Gene

hemG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX using menaquinone as electron acceptor.

Catalytic activityi

Protoporphyrinogen IX + 3 menaquinone = protoporphyrin IX + 3 menaquinol.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen IX dehydrogenase [menaquinone] (hemG)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • electron transfer activity Source: InterPro
  • FMN binding Source: EcoCyc
  • menaquinone-dependent protoporphyrinogen oxidase activity Source: EcoCyc
  • oxygen-dependent protoporphyrinogen oxidase activity Source: EcoCyc

GO - Biological processi

  • heme biosynthetic process Source: EcoCyc
  • porphyrin-containing compound biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processPorphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:PROTOPORGENOXI-MONOMER
MetaCyc:PROTOPORGENOXI-MONOMER
UniPathwayiUPA00251; UER00324

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen IX dehydrogenase [menaquinone] (EC:1.3.5.3)
Gene namesi
Name:hemG
Synonyms:yihB
Ordered Locus Names:b3850, JW3827
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11485 hemG

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352591 – 181Protoporphyrinogen IX dehydrogenase [menaquinone]Add BLAST181

Proteomic databases

PaxDbiP0ACB4
PRIDEiP0ACB4

Interactioni

Subunit structurei

Belongs to a multi-protein complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
frdAP003632EBI-1115706,EBI-550480

Protein-protein interaction databases

BioGridi4262620, 2 interactors
DIPiDIP-35890N
IntActiP0ACB4, 19 interactors
STRINGi316385.ECDH10B_4039

Structurei

3D structure databases

ProteinModelPortaliP0ACB4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 172Flavodoxin-likePROSITE-ProRule annotationAdd BLAST170

Phylogenomic databases

eggNOGiENOG4108RR8 Bacteria
COG4635 LUCA
HOGENOMiHOG000251295
InParanoidiP0ACB4
KOiK00230
OMAiIEYTDWE
PhylomeDBiP0ACB4

Family and domain databases

Gene3Di3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR008254 Flavodoxin/NO_synth
IPR001226 Flavodoxin_CS
IPR026816 Flavodoxin_dom
IPR029039 Flavoprotein-like_sf
PfamiView protein in Pfam
PF12724 Flavodoxin_5, 1 hit
SUPFAMiSSF52218 SSF52218, 1 hit
PROSITEiView protein in PROSITE
PS00201 FLAVODOXIN, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLILFSTR DGQTREIASY LASELKELGI QADVANVHRI EEPQWENYDR
60 70 80 90 100
VVIGASIRYG HYHSAFQEFV KKHATRLNSM PSAFYSVNLV ARKPEKRTPQ
110 120 130 140 150
TNSYARKFLM NSQWRPDRCA VIAGALRYPR YRWYDRFMIK LIMKMSGGET
160 170 180
DTRKEVVYTD WEQVANFARE IAHLTDKPTL K
Length:181
Mass (Da):21,226
Last modified:November 8, 2005 - v1
Checksum:iBCCB5AE594010026
GO

Sequence cautioni

The sequence X54687 differs from that shown. Reason: Frameshift at position 159.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54687 Genomic DNA No translation available.
X68660 Genomic DNA Translation: CAA48626.1
M87049 Genomic DNA Translation: AAA67647.1
U00096 Genomic DNA Translation: AAC76853.1
AP009048 Genomic DNA Translation: BAE77453.1
PIRiJC2513
RefSeqiNP_418292.1, NC_000913.3
WP_000853959.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76853; AAC76853; b3850
BAE77453; BAE77453; BAE77453
GeneIDi948331
KEGGiecj:JW3827
eco:b3850
PATRICifig|1411691.4.peg.2860

Similar proteinsi

Entry informationi

Entry nameiHEMG_ECOLI
AccessioniPrimary (citable) accession number: P0ACB4
Secondary accession number(s): P27863, Q2M8F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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