Replicative DNA helicase - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0ACB0 (DNAB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Gene names

Name:	dnaB
Synonyms:	groP, grpA
Ordered Locus Names:	b4052, JW4012

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW DNA unwinding involved in replication Inferred from direct assay PubMed 3007474. Source: EcoCyc
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from direct assay PubMed 3007474. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		3	EBI-548978,EBI-548978
dnaA	P03004	4	EBI-548978,EBI-548951
dnaC	P0AEF0	2	EBI-548978,EBI-549012
dnaG	P0ABS5	2	EBI-548978,EBI-549259
dnaX	P06710	2	EBI-548978,EBI-549140

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 471

471

Replicative DNA helicase

PRO_0000102019

Regions

Domain

200 – 467

268

SF4 helicase

Nucleotide binding

231 – 238

ATP Probable

Secondary structure

471

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0ACB0 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 5B5ED3625A7F7DE5
Show »

FASTA

471

52,390

        10         20         30         40         50         60 
MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD VAERVVADDF 

        70         80         90        100        110        120 
YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV GGFAYLAELS KNTPSAANIS 

       130        140        150        160        170        180 
AYADIVRERA VVREMISVAN EIAEAGFDPQ GRTSEDLLDL AESRVFKIAE SRANKDEGPK 

       190        200        210        220        230        240 
NIADVLDATV ARIEQLFQQP HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF 

       250        260        270        280        290        300 
AMNLVENAAM LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT 

       310        320        330        340        350        360 
MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV PALSDNRTLE 

       370        380        390        400        410        420 
IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS DLRESGSIEQ DADLIMFIYR 

       430        440        450        460        470 
DEVYHENSDL KGIAEIIIGK QRNGPIGTVR LTFNGQWSRF DNYAGPQYDD E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli." Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K. J. Biol. Chem. 259:97-101(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"E. coli homolog of zeta crystallin." Dixon N.E., Lilley P.E. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157. Strain: K12.
[6]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[7]	"NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer." Weigelt J., Brown S.E., Miles C.S., Dixon N.E., Otting G. Structure 7:681-690(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 24-137.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K01174 Genomic DNA. Translation: AAA23689.1.
L02312 Genomic DNA. Translation: AAA23690.1.
U00006 Genomic DNA. Translation: AAC43146.1.
U00096 Genomic DNA. Translation: AAC77022.1.
AP009048 Genomic DNA. Translation: BAE78054.1.

PIR

IQECDB. C65213.

RefSeq

NP_418476.1. NC_000913.2.
YP_492195.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B79	X-ray	2.30	A/B/C/D	16-129	[»]
1JWE	NMR	-	A	25-137	[»]

ProteinModelPortal

P0ACB0.

SMR

P0ACB0. Positions 25-462.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35913N.

IntAct

P0ACB0. 32 interactions.

MINT

MINT-584605.

STRING

511145.b4052.

2D gel databases

SWISS-2DPAGE

P0ACB0.

Proteomic databases

PaxDb

P0ACB0.

PRIDE

P0ACB0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77022; AAC77022; b4052.
BAE78054; BAE78054; BAE78054.

GeneID

12932658.
948555.

KEGG

ecj:Y75_p3939.
eco:b4052.

PATRIC

32123643. VBIEscCol129921_4170.

Organism-specific databases

EchoBASE

EB0232.

EcoGene

EG10236. dnaB.

Phylogenomic databases

eggNOG

COG0305.

HOGENOM

HOG000113196.

K02314.

OMA

YRTALML.

ProtClustDB

PRK08006.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10236-MONOMER.
ECOL316407:JW4012-MONOMER.
MetaCyc:EG10236-MONOMER.

Gene expression databases

Genevestigator

P0ACB0.

Family and domain databases

Gene3D

1.10.860.10. 1 hit.

InterPro

IPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
[Graphical view]

Pfam

PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

SUPFAM

SSF48024. DNA_helicase_DnaB-like_N. 1 hit.

TIGRFAMs

TIGR00665. DnaB. 1 hit.

PROSITE

PS51199. SF4_HELICASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0ACB0.

Entry information

Entry name

DNAB_ECOLI

Accession

Primary (citable) accession number: P0ACB0
Secondary accession number(s): P03005, Q2M6Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents