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P0ACB0

- DNAB_ECOLI

UniProt

P0ACB0 - DNAB_ECOLI

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Protein

Replicative DNA helicase

Gene
dnaB, groP, grpA, b4052, JW4012
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2388ATP Inferred

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: EcoCyc
  4. helicase activity Source: EcoCyc
  5. identical protein binding Source: IntAct
  6. protein binding Source: IntAct

GO - Biological processi

  1. DNA replication Source: EcoCyc
  2. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  3. DNA unwinding involved in DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10236-MONOMER.
ECOL316407:JW4012-MONOMER.
MetaCyc:EG10236-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Gene namesi
Name:dnaB
Synonyms:groP, grpA
Ordered Locus Names:b4052, JW4012
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10236. dnaB.

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Replicative DNA helicasePRO_0000102019Add
BLAST

Proteomic databases

PaxDbiP0ACB0.
PRIDEiP0ACB0.

2D gel databases

SWISS-2DPAGEP0ACB0.

Expressioni

Gene expression databases

GenevestigatoriP0ACB0.

Interactioni

Subunit structurei

Homohexamer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-548978,EBI-548978
dnaAP030044EBI-548978,EBI-548951
dnaCP0AEF09EBI-548978,EBI-549012
dnaGP0ABS53EBI-548978,EBI-549259
dnaXP067102EBI-548978,EBI-549140
priCP238625EBI-548978,EBI-1117383
repP099803EBI-548978,EBI-6558011

Protein-protein interaction databases

DIPiDIP-35913N.
IntActiP0ACB0. 38 interactions.
MINTiMINT-584605.
STRINGi511145.b4052.

Structurei

Secondary structure

1
471
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 4314
Helixi45 – 473
Helixi48 – 525
Helixi57 – 593
Beta strandi60 – 623
Helixi63 – 7715
Helixi84 – 929
Turni93 – 964
Helixi97 – 1004
Helixi102 – 11211
Beta strandi115 – 1173
Helixi119 – 1279

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B79X-ray2.30A/B/C/D16-129[»]
1JWENMR-A24-137[»]
ProteinModelPortaliP0ACB0.
SMRiP0ACB0. Positions 25-462.

Miscellaneous databases

EvolutionaryTraceiP0ACB0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini200 – 467268SF4 helicaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0305.
HOGENOMiHOG000113196.
KOiK02314.
OMAiPADQIMM.
OrthoDBiEOG6T4RW5.
PhylomeDBiP0ACB0.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACB0-1 [UniParc]FASTAAdd to Basket

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MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD    50
VAERVVADDF YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV 100
GGFAYLAELS KNTPSAANIS AYADIVRERA VVREMISVAN EIAEAGFDPQ 150
GRTSEDLLDL AESRVFKIAE SRANKDEGPK NIADVLDATV ARIEQLFQQP 200
HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF AMNLVENAAM 250
LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT 300
MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV 350
PALSDNRTLE IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS 400
DLRESGSIEQ DADLIMFIYR DEVYHENSDL KGIAEIIIGK QRNGPIGTVR 450
LTFNGQWSRF DNYAGPQYDD E 471
Length:471
Mass (Da):52,390
Last modified:August 13, 1987 - v1
Checksum:i5B5ED3625A7F7DE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01174 Genomic DNA. Translation: AAA23689.1.
L02312 Genomic DNA. Translation: AAA23690.1.
U00006 Genomic DNA. Translation: AAC43146.1.
U00096 Genomic DNA. Translation: AAC77022.1.
AP009048 Genomic DNA. Translation: BAE78054.1.
PIRiC65213. IQECDB.
RefSeqiNP_418476.1. NC_000913.3.
YP_492195.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77022; AAC77022; b4052.
BAE78054; BAE78054; BAE78054.
GeneIDi12932658.
948555.
KEGGiecj:Y75_p3939.
eco:b4052.
PATRICi32123643. VBIEscCol129921_4170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01174 Genomic DNA. Translation: AAA23689.1 .
L02312 Genomic DNA. Translation: AAA23690.1 .
U00006 Genomic DNA. Translation: AAC43146.1 .
U00096 Genomic DNA. Translation: AAC77022.1 .
AP009048 Genomic DNA. Translation: BAE78054.1 .
PIRi C65213. IQECDB.
RefSeqi NP_418476.1. NC_000913.3.
YP_492195.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B79 X-ray 2.30 A/B/C/D 16-129 [» ]
1JWE NMR - A 24-137 [» ]
ProteinModelPortali P0ACB0.
SMRi P0ACB0. Positions 25-462.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35913N.
IntActi P0ACB0. 38 interactions.
MINTi MINT-584605.
STRINGi 511145.b4052.

2D gel databases

SWISS-2DPAGE P0ACB0.

Proteomic databases

PaxDbi P0ACB0.
PRIDEi P0ACB0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77022 ; AAC77022 ; b4052 .
BAE78054 ; BAE78054 ; BAE78054 .
GeneIDi 12932658.
948555.
KEGGi ecj:Y75_p3939.
eco:b4052.
PATRICi 32123643. VBIEscCol129921_4170.

Organism-specific databases

EchoBASEi EB0232.
EcoGenei EG10236. dnaB.

Phylogenomic databases

eggNOGi COG0305.
HOGENOMi HOG000113196.
KOi K02314.
OMAi PADQIMM.
OrthoDBi EOG6T4RW5.
PhylomeDBi P0ACB0.

Enzyme and pathway databases

BioCyci EcoCyc:EG10236-MONOMER.
ECOL316407:JW4012-MONOMER.
MetaCyc:EG10236-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0ACB0.
PROi P0ACB0.

Gene expression databases

Genevestigatori P0ACB0.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
PROSITEi PS51199. SF4_HELICASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli."
    Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K.
    J. Biol. Chem. 259:97-101(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "E. coli homolog of zeta crystallin."
    Dixon N.E., Lilley P.E.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
    Strain: K12.
  6. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  7. "NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer."
    Weigelt J., Brown S.E., Miles C.S., Dixon N.E., Otting G.
    Structure 7:681-690(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-137.

Entry informationi

Entry nameiDNAB_ECOLI
AccessioniPrimary (citable) accession number: P0ACB0
Secondary accession number(s): P03005, Q2M6Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3