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P0ACB0

- DNAB_ECOLI

UniProt

P0ACB0 - DNAB_ECOLI

Protein

Replicative DNA helicase

Gene

dnaB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi231 – 2388ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: EcoCyc
    4. helicase activity Source: EcoCyc
    5. identical protein binding Source: IntAct
    6. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication Source: EcoCyc
    2. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    3. DNA unwinding involved in DNA replication Source: EcoCyc
    4. response to ionizing radiation Source: EcoCyc

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10236-MONOMER.
    ECOL316407:JW4012-MONOMER.
    MetaCyc:EG10236-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicative DNA helicase (EC:3.6.4.12)
    Gene namesi
    Name:dnaB
    Synonyms:groP, grpA
    Ordered Locus Names:b4052, JW4012
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10236. dnaB.

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 471471Replicative DNA helicasePRO_0000102019Add
    BLAST

    Proteomic databases

    PaxDbiP0ACB0.
    PRIDEiP0ACB0.

    2D gel databases

    SWISS-2DPAGEP0ACB0.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACB0.

    Interactioni

    Subunit structurei

    Homohexamer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-548978,EBI-548978
    dnaAP030044EBI-548978,EBI-548951
    dnaCP0AEF09EBI-548978,EBI-549012
    dnaGP0ABS53EBI-548978,EBI-549259
    dnaXP067102EBI-548978,EBI-549140
    priCP238625EBI-548978,EBI-1117383
    repP099803EBI-548978,EBI-6558011

    Protein-protein interaction databases

    DIPiDIP-35913N.
    IntActiP0ACB0. 38 interactions.
    MINTiMINT-584605.
    STRINGi511145.b4052.

    Structurei

    Secondary structure

    1
    471
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 4314
    Helixi45 – 473
    Helixi48 – 525
    Helixi57 – 593
    Beta strandi60 – 623
    Helixi63 – 7715
    Helixi84 – 929
    Turni93 – 964
    Helixi97 – 1004
    Helixi102 – 11211
    Beta strandi115 – 1173
    Helixi119 – 1279

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B79X-ray2.30A/B/C/D16-129[»]
    1JWENMR-A24-137[»]
    ProteinModelPortaliP0ACB0.
    SMRiP0ACB0. Positions 25-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0ACB0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini200 – 467268SF4 helicasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0305.
    HOGENOMiHOG000113196.
    KOiK02314.
    OMAiPADQIMM.
    OrthoDBiEOG6T4RW5.
    PhylomeDBiP0ACB0.

    Family and domain databases

    Gene3Di1.10.860.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF48024. SSF48024. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00665. DnaB. 1 hit.
    PROSITEiPS51199. SF4_HELICASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACB0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD    50
    VAERVVADDF YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV 100
    GGFAYLAELS KNTPSAANIS AYADIVRERA VVREMISVAN EIAEAGFDPQ 150
    GRTSEDLLDL AESRVFKIAE SRANKDEGPK NIADVLDATV ARIEQLFQQP 200
    HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF AMNLVENAAM 250
    LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT 300
    MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV 350
    PALSDNRTLE IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS 400
    DLRESGSIEQ DADLIMFIYR DEVYHENSDL KGIAEIIIGK QRNGPIGTVR 450
    LTFNGQWSRF DNYAGPQYDD E 471
    Length:471
    Mass (Da):52,390
    Last modified:August 13, 1987 - v1
    Checksum:i5B5ED3625A7F7DE5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01174 Genomic DNA. Translation: AAA23689.1.
    L02312 Genomic DNA. Translation: AAA23690.1.
    U00006 Genomic DNA. Translation: AAC43146.1.
    U00096 Genomic DNA. Translation: AAC77022.1.
    AP009048 Genomic DNA. Translation: BAE78054.1.
    PIRiC65213. IQECDB.
    RefSeqiNP_418476.1. NC_000913.3.
    YP_492195.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77022; AAC77022; b4052.
    BAE78054; BAE78054; BAE78054.
    GeneIDi12932658.
    948555.
    KEGGiecj:Y75_p3939.
    eco:b4052.
    PATRICi32123643. VBIEscCol129921_4170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01174 Genomic DNA. Translation: AAA23689.1 .
    L02312 Genomic DNA. Translation: AAA23690.1 .
    U00006 Genomic DNA. Translation: AAC43146.1 .
    U00096 Genomic DNA. Translation: AAC77022.1 .
    AP009048 Genomic DNA. Translation: BAE78054.1 .
    PIRi C65213. IQECDB.
    RefSeqi NP_418476.1. NC_000913.3.
    YP_492195.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B79 X-ray 2.30 A/B/C/D 16-129 [» ]
    1JWE NMR - A 24-137 [» ]
    ProteinModelPortali P0ACB0.
    SMRi P0ACB0. Positions 25-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35913N.
    IntActi P0ACB0. 38 interactions.
    MINTi MINT-584605.
    STRINGi 511145.b4052.

    2D gel databases

    SWISS-2DPAGE P0ACB0.

    Proteomic databases

    PaxDbi P0ACB0.
    PRIDEi P0ACB0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77022 ; AAC77022 ; b4052 .
    BAE78054 ; BAE78054 ; BAE78054 .
    GeneIDi 12932658.
    948555.
    KEGGi ecj:Y75_p3939.
    eco:b4052.
    PATRICi 32123643. VBIEscCol129921_4170.

    Organism-specific databases

    EchoBASEi EB0232.
    EcoGenei EG10236. dnaB.

    Phylogenomic databases

    eggNOGi COG0305.
    HOGENOMi HOG000113196.
    KOi K02314.
    OMAi PADQIMM.
    OrthoDBi EOG6T4RW5.
    PhylomeDBi P0ACB0.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10236-MONOMER.
    ECOL316407:JW4012-MONOMER.
    MetaCyc:EG10236-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0ACB0.
    PROi P0ACB0.

    Gene expression databases

    Genevestigatori P0ACB0.

    Family and domain databases

    Gene3Di 1.10.860.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48024. SSF48024. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00665. DnaB. 1 hit.
    PROSITEi PS51199. SF4_HELICASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli."
      Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K.
      J. Biol. Chem. 259:97-101(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
      Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
      Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "E. coli homolog of zeta crystallin."
      Dixon N.E., Lilley P.E.
      Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
      Strain: K12.
    6. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    7. "NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer."
      Weigelt J., Brown S.E., Miles C.S., Dixon N.E., Otting G.
      Structure 7:681-690(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 24-137.

    Entry informationi

    Entry nameiDNAB_ECOLI
    AccessioniPrimary (citable) accession number: P0ACB0
    Secondary accession number(s): P03005, Q2M6Q2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3