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P0ACB0 (DNAB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicative DNA helicase

EC=3.6.4.12
Gene names
Name:dnaB
Synonyms:groP, grpA
Ordered Locus Names:b4052, JW4012
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Homohexamer.

Sequence similarities

Belongs to the helicase family. DnaB subfamily.

Contains 1 SF4 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Replicative DNA helicase
PRO_0000102019

Regions

Domain200 – 467268SF4 helicase
Nucleotide binding231 – 2388ATP Probable

Secondary structure

.................... 471
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ACB0 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 5B5ED3625A7F7DE5

FASTA47152,390
        10         20         30         40         50         60 
MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD VAERVVADDF 

        70         80         90        100        110        120 
YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV GGFAYLAELS KNTPSAANIS 

       130        140        150        160        170        180 
AYADIVRERA VVREMISVAN EIAEAGFDPQ GRTSEDLLDL AESRVFKIAE SRANKDEGPK 

       190        200        210        220        230        240 
NIADVLDATV ARIEQLFQQP HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF 

       250        260        270        280        290        300 
AMNLVENAAM LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT 

       310        320        330        340        350        360 
MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV PALSDNRTLE 

       370        380        390        400        410        420 
IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS DLRESGSIEQ DADLIMFIYR 

       430        440        450        460        470 
DEVYHENSDL KGIAEIIIGK QRNGPIGTVR LTFNGQWSRF DNYAGPQYDD E 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli."
Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K.
J. Biol. Chem. 259:97-101(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"E. coli homolog of zeta crystallin."
Dixon N.E., Lilley P.E.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
Strain: K12.
[6]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[7]"NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer."
Weigelt J., Brown S.E., Miles C.S., Dixon N.E., Otting G.
Structure 7:681-690(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 24-137.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01174 Genomic DNA. Translation: AAA23689.1.
L02312 Genomic DNA. Translation: AAA23690.1.
U00006 Genomic DNA. Translation: AAC43146.1.
U00096 Genomic DNA. Translation: AAC77022.1.
AP009048 Genomic DNA. Translation: BAE78054.1.
PIRIQECDB. C65213.
RefSeqNP_418476.1. NC_000913.3.
YP_492195.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B79X-ray2.30A/B/C/D16-129[»]
1JWENMR-A24-137[»]
ProteinModelPortalP0ACB0.
SMRP0ACB0. Positions 25-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35913N.
IntActP0ACB0. 38 interactions.
MINTMINT-584605.
STRING511145.b4052.

2D gel databases

SWISS-2DPAGEP0ACB0.

Proteomic databases

PaxDbP0ACB0.
PRIDEP0ACB0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77022; AAC77022; b4052.
BAE78054; BAE78054; BAE78054.
GeneID12932658.
948555.
KEGGecj:Y75_p3939.
eco:b4052.
PATRIC32123643. VBIEscCol129921_4170.

Organism-specific databases

EchoBASEEB0232.
EcoGeneEG10236. dnaB.

Phylogenomic databases

eggNOGCOG0305.
HOGENOMHOG000113196.
KOK02314.
OMAPADQIMM.
OrthoDBEOG6T4RW5.
PhylomeDBP0ACB0.

Enzyme and pathway databases

BioCycEcoCyc:EG10236-MONOMER.
ECOL316407:JW4012-MONOMER.
MetaCyc:EG10236-MONOMER.

Gene expression databases

GenevestigatorP0ACB0.

Family and domain databases

Gene3D1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR00665. DnaB. 1 hit.
PROSITEPS51199. SF4_HELICASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0ACB0.
PROP0ACB0.

Entry information

Entry nameDNAB_ECOLI
AccessionPrimary (citable) accession number: P0ACB0
Secondary accession number(s): P03005, Q2M6Q2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene