P0ACA9 (GSTB_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase GstB
EC=2.5.1.18

Gene names

Name:	gstB
Ordered Locus Names:	SF0788, S0831

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAN42421.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAP16296.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Glutathione S-transferase GstB		PRO_0000186020
Regions
	Domain	1 – 83	83	GST N-terminal
	Domain	88 – 208	121	GST C-terminal
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
	Binding site	39	1	Glutathione By similarity
	Binding site	53	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ACA9 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8AD199E313B05094	FASTA	208	23,713
	10 20 30 40 50 60 MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN GLVPLLRDDE 70 80 90 100 110 120 SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD WANQTLSNAH RGILMGLVRT 130 140 150 160 170 180 PPEERDQAAI DASCKECDAL FALLDAELAK VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW 190 200 TPRPNLQRWY QQLTERPAVR KVVMIPVS

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN42421.1. Different initiation. AE014073 Genomic DNA. Translation: AAP16296.1. Different initiation.
RefSeq	NP_706714.1. NC_004337.2. NP_836490.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0ACA9.
SMR	P0ACA9. Positions 6-201.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF0788.
Proteomic databases
PaxDb	P0ACA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN42421; AAN42421; SF0788. AAP16296; AAP16296; S0831.
GeneID	1023726. 1077248.
KEGG	sfl:SF0788. sfx:S0831.
PATRIC	18702773. VBIShiFle31049_0896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125757.
ProtClustDB	CLSK879786.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTB_SHIFL

Accession

Primary (citable) accession number: P0ACA9
Secondary accession number(s): P75805, Q9R7R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program