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Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121GlutathioneBy similarity
Binding sitei39 – 391GlutathioneBy similarity
Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase GstB (EC:2.5.1.18)
Gene namesi
Name:gstB
Ordered Locus Names:c0923
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Glutathione S-transferase GstBPRO_0000186019Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi199310.c0923.

Structurei

3D structure databases

ProteinModelPortaliP0ACA8.
SMRiP0ACA8. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8383GST N-terminalAdd
BLAST
Domaini88 – 208121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 682Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

HOGENOMiHOG000125757.
OMAiDIAFAPW.
OrthoDBiEOG696BVR.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
60 70 80 90 100
GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
110 120 130 140 150
WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
160 170 180 190 200
VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR

KVVMIPVS
Length:208
Mass (Da):23,713
Last modified:November 8, 2005 - v1
Checksum:i8AD199E313B05094
GO

Sequence cautioni

The sequence AAN79396.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79396.1. Different initiation.
RefSeqiNP_752853.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN79396; AAN79396; c0923.
GeneIDi1038015.
KEGGiecc:c0923.
PATRICi18279895. VBIEscCol75197_0877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79396.1. Different initiation.
RefSeqiNP_752853.1. NC_004431.1.

3D structure databases

ProteinModelPortaliP0ACA8.
SMRiP0ACA8. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN79396; AAN79396; c0923.
GeneIDi1038015.
KEGGiecc:c0923.
PATRICi18279895. VBIEscCol75197_0877.

Phylogenomic databases

HOGENOMiHOG000125757.
OMAiDIAFAPW.
OrthoDBiEOG696BVR.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiGSTB_ECOL6
AccessioniPrimary (citable) accession number: P0ACA8
Secondary accession number(s): P75805, Q9R7R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.