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Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=5 mM for bromoacetate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity
    Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    • glutathione transferase activity Source: EcoCyc
    • identical protein binding Source: IntAct

    GO - Biological processi

    • metabolic process Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase GstB (EC:2.5.1.18)
    Gene namesi
    Name:gstB
    Synonyms:yliJ
    Ordered Locus Names:b0838, JW0822
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13481. gstB.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are hypersensitive to bromoacetate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 208208Glutathione S-transferase GstBPRO_0000186018Add
    BLAST

    Proteomic databases

    PaxDbiP0ACA7.
    PRIDEiP0ACA7.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1120568,EBI-1120568
    hisBP069874EBI-1120568,EBI-1126930

    Protein-protein interaction databases

    DIPiDIP-48220N.
    IntActiP0ACA7. 2 interactions.
    STRINGi511145.b0838.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACA7.
    SMRiP0ACA7. Positions 1-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 8383GST N-terminalAdd
    BLAST
    Domaini88 – 208121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125757.
    InParanoidiP0ACA7.
    OMAiDIAFAPW.
    OrthoDBiEOG696BVR.
    PhylomeDBiP0ACA7.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACA7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
    60 70 80 90 100
    GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
    110 120 130 140 150
    WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
    160 170 180 190 200
    VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR

    KVVMIPVS
    Length:208
    Mass (Da):23,713
    Last modified:November 8, 2005 - v1
    Checksum:i8AD199E313B05094
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2.
    AP009048 Genomic DNA. Translation: BAA35541.1.
    RefSeqiNP_415359.4. NC_000913.3.
    WP_001295292.1. NZ_CP010445.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838.
    BAA35541; BAA35541; BAA35541.
    GeneIDi945469.
    KEGGieco:b0838.
    PATRICi32116879. VBIEscCol129921_0865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2.
    AP009048 Genomic DNA. Translation: BAA35541.1.
    RefSeqiNP_415359.4. NC_000913.3.
    WP_001295292.1. NZ_CP010445.1.

    3D structure databases

    ProteinModelPortaliP0ACA7.
    SMRiP0ACA7. Positions 1-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-48220N.
    IntActiP0ACA7. 2 interactions.
    STRINGi511145.b0838.

    Proteomic databases

    PaxDbiP0ACA7.
    PRIDEiP0ACA7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838.
    BAA35541; BAA35541; BAA35541.
    GeneIDi945469.
    KEGGieco:b0838.
    PATRICi32116879. VBIEscCol129921_0865.

    Organism-specific databases

    EchoBASEiEB3254.
    EcoGeneiEG13481. gstB.

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125757.
    InParanoidiP0ACA7.
    OMAiDIAFAPW.
    OrthoDBiEOG696BVR.
    PhylomeDBiP0ACA7.

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Miscellaneous databases

    PROiP0ACA7.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate."
      Desai K.K., Miller B.G.
      Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

    Entry informationi

    Entry nameiGSTB_ECOLI
    AccessioniPrimary (citable) accession number: P0ACA7
    Secondary accession number(s): P75805, Q9R7R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: July 22, 2015
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.