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P0ACA7 (GSTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase GstB

EC=2.5.1.18
Gene names
Name:gstB
Synonyms:yliJ
Ordered Locus Names:b0838, JW0822
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate. Ref.4

Catalytic activity

RX + glutathione = HX + R-S-glutathione. Ref.4

Disruption phenotype

Mutants are hypersensitive to bromoacetate. Ref.4

Sequence similarities

Belongs to the GST superfamily.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Biophysicochemical properties

Kinetic parameters:

KM=5 mM for bromoacetate Ref.4

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-1120568,EBI-1120568
hisBP069874EBI-1120568,EBI-1126930

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 208208Glutathione S-transferase GstB
PRO_0000186018

Regions

Domain1 – 8383GST N-terminal
Domain88 – 208121GST C-terminal
Region67 – 682Glutathione binding By similarity

Sites

Binding site121Glutathione By similarity
Binding site391Glutathione By similarity
Binding site531Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0ACA7 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 8AD199E313B05094

FASTA20823,713
        10         20         30         40         50         60 
MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN GLVPLLRDDE 

        70         80         90        100        110        120 
SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD WANQTLSNAH RGILMGLVRT 

       130        140        150        160        170        180 
PPEERDQAAI DASCKECDAL FALLDAELAK VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW 

       190        200 
TPRPNLQRWY QQLTERPAVR KVVMIPVS 

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate."
Desai K.K., Miller B.G.
Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC73925.2.
AP009048 Genomic DNA. Translation: BAA35541.1.
RefSeqNP_415359.4. NC_000913.3.
YP_489111.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0ACA7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48220N.
IntActP0ACA7. 2 interactions.
STRING511145.b0838.

Proteomic databases

PaxDbP0ACA7.
PRIDEP0ACA7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73925; AAC73925; b0838.
BAA35541; BAA35541; BAA35541.
GeneID12933830.
945469.
KEGGecj:Y75_p0811.
eco:b0838.
PATRIC32116879. VBIEscCol129921_0865.

Organism-specific databases

EchoBASEEB3254.
EcoGeneEG13481. gstB.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125757.
OMADIAFAPW.
OrthoDBEOG696BVR.
PhylomeDBP0ACA7.

Enzyme and pathway databases

BioCycEcoCyc:G6438-MONOMER.
ECOL316407:JW0822-MONOMER.
MetaCyc:G6438-MONOMER.

Gene expression databases

GenevestigatorP0ACA7.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0ACA7.

Entry information

Entry nameGSTB_ECOLI
AccessionPrimary (citable) accession number: P0ACA7
Secondary accession number(s): P75805, Q9R7R4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene