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Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=5 mM for bromoacetate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121GlutathioneBy similarity
Binding sitei39 – 391GlutathioneBy similarity
Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: EcoCyc
  2. identical protein binding Source: IntAct

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6438-MONOMER.
ECOL316407:JW0822-MONOMER.
MetaCyc:G6438-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase GstB (EC:2.5.1.18)
Gene namesi
Name:gstB
Synonyms:yliJ
Ordered Locus Names:b0838, JW0822
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13481. gstB.

Pathology & Biotechi

Disruption phenotypei

Mutants are hypersensitive to bromoacetate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Glutathione S-transferase GstBPRO_0000186018Add
BLAST

Proteomic databases

PaxDbiP0ACA7.
PRIDEiP0ACA7.

Expressioni

Gene expression databases

GenevestigatoriP0ACA7.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1120568,EBI-1120568
hisBP069874EBI-1120568,EBI-1126930

Protein-protein interaction databases

DIPiDIP-48220N.
IntActiP0ACA7. 2 interactions.
STRINGi511145.b0838.

Structurei

3D structure databases

ProteinModelPortaliP0ACA7.
SMRiP0ACA7. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8383GST N-terminalAdd
BLAST
Domaini88 – 208121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 682Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125757.
InParanoidiP0ACA7.
OMAiDIAFAPW.
OrthoDBiEOG696BVR.
PhylomeDBiP0ACA7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACA7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
60 70 80 90 100
GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
110 120 130 140 150
WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
160 170 180 190 200
VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR

KVVMIPVS
Length:208
Mass (Da):23,713
Last modified:November 8, 2005 - v1
Checksum:i8AD199E313B05094
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73925.2.
AP009048 Genomic DNA. Translation: BAA35541.1.
RefSeqiNP_415359.4. NC_000913.3.
YP_489111.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73925; AAC73925; b0838.
BAA35541; BAA35541; BAA35541.
GeneIDi12933830.
945469.
KEGGiecj:Y75_p0811.
eco:b0838.
PATRICi32116879. VBIEscCol129921_0865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73925.2.
AP009048 Genomic DNA. Translation: BAA35541.1.
RefSeqiNP_415359.4. NC_000913.3.
YP_489111.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0ACA7.
SMRiP0ACA7. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48220N.
IntActiP0ACA7. 2 interactions.
STRINGi511145.b0838.

Proteomic databases

PaxDbiP0ACA7.
PRIDEiP0ACA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73925; AAC73925; b0838.
BAA35541; BAA35541; BAA35541.
GeneIDi12933830.
945469.
KEGGiecj:Y75_p0811.
eco:b0838.
PATRICi32116879. VBIEscCol129921_0865.

Organism-specific databases

EchoBASEiEB3254.
EcoGeneiEG13481. gstB.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125757.
InParanoidiP0ACA7.
OMAiDIAFAPW.
OrthoDBiEOG696BVR.
PhylomeDBiP0ACA7.

Enzyme and pathway databases

BioCyciEcoCyc:G6438-MONOMER.
ECOL316407:JW0822-MONOMER.
MetaCyc:G6438-MONOMER.

Miscellaneous databases

PROiP0ACA7.

Gene expression databases

GenevestigatoriP0ACA7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate."
    Desai K.K., Miller B.G.
    Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

Entry informationi

Entry nameiGSTB_ECOLI
AccessioniPrimary (citable) accession number: P0ACA7
Secondary accession number(s): P75805, Q9R7R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.