Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=5 mM for bromoacetate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei12GlutathioneBy similarity1
    Binding sitei39GlutathioneBy similarity1
    Binding sitei53Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

    GO - Molecular functioni

    • arsenate reductase activity Source: EcoCyc
    • glutathione transferase activity Source: EcoCyc
    • identical protein binding Source: IntAct

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER
    MetaCyc:G6438-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase GstB (EC:2.5.1.18)
    Gene namesi
    Name:gstB
    Synonyms:yliJ
    Ordered Locus Names:b0838, JW0822
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13481 gstB

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are hypersensitive to bromoacetate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001860181 – 208Glutathione S-transferase GstBAdd BLAST208

    Proteomic databases

    EPDiP0ACA7
    PaxDbiP0ACA7
    PRIDEiP0ACA7

    Interactioni

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • identical protein binding Source: IntAct

    Protein-protein interaction databases

    BioGridi426282414 interactors.
    DIPiDIP-48220N
    IntActiP0ACA7 2 interactors.
    STRINGi316385.ECDH10B_0907

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACA7
    SMRiP0ACA7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 83GST N-terminalAdd BLAST83
    Domaini88 – 208GST C-terminalAdd BLAST121

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni67 – 68Glutathione bindingBy similarity2

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4108TGY Bacteria
    COG0625 LUCA
    HOGENOMiHOG000125757
    InParanoidiP0ACA7
    KOiK00799
    OMAiLWQKAFE
    PhylomeDBiP0ACA7

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987 Glutathione-S-Trfase_C-like
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR004046 GST_C
    IPR036249 Thioredoxin-like_sf
    PfamiView protein in Pfam
    PF00043 GST_C, 1 hit
    PF02798 GST_N, 1 hit
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 1 hit
    PROSITEiView protein in PROSITE
    PS50405 GST_CTER, 1 hit
    PS50404 GST_NTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0ACA7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
    60 70 80 90 100
    GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
    110 120 130 140 150
    WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
    160 170 180 190 200
    VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR

    KVVMIPVS
    Length:208
    Mass (Da):23,713
    Last modified:November 8, 2005 - v1
    Checksum:i8AD199E313B05094
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC73925.2
    AP009048 Genomic DNA Translation: BAA35541.1
    RefSeqiNP_415359.4, NC_000913.3
    WP_001295292.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838
    BAA35541; BAA35541; BAA35541
    GeneIDi945469
    KEGGiecj:JW0822
    eco:b0838
    PATRICifig|1411691.4.peg.1440

    Similar proteinsi

    Entry informationi

    Entry nameiGSTB_ECOLI
    AccessioniPrimary (citable) accession number: P0ACA7
    Secondary accession number(s): P75805, Q9R7R4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: March 28, 2018
    This is version 96 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome