P0ACA7 (GSTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase GstB
EC=2.5.1.18

Gene names

Name:	gstB
Synonyms:	yliJ
Ordered Locus Names:	b0838, JW0822

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate. Ref.4
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.4
Disruption phenotype	Mutants are hypersensitive to bromoacetate. Ref.4
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Biophysicochemical properties	Kinetic parameters: KM=5 mM for bromoacetate Ref.4

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Glutathione S-transferase GstB		PRO_0000186018
Regions
	Domain	1 – 83	83	GST N-terminal
	Domain	88 – 208	121	GST C-terminal
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
	Binding site	39	1	Glutathione By similarity
	Binding site	53	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ACA7 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8AD199E313B05094	FASTA	208	23,713
	10 20 30 40 50 60 MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN GLVPLLRDDE 70 80 90 100 110 120 SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD WANQTLSNAH RGILMGLVRT 130 140 150 160 170 180 PPEERDQAAI DASCKECDAL FALLDAELAK VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW 190 200 TPRPNLQRWY QQLTERPAVR KVVMIPVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate." Desai K.K., Miller B.G. Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73925.2. AP009048 Genomic DNA. Translation: BAA35541.1.
RefSeq	NP_415359.4. NC_000913.2. YP_489111.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0ACA7.
SMR	P0ACA7. Positions 6-201.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48220N.
IntAct	P0ACA7. 1 interaction.
STRING	511145.b0838.
Proteomic databases
PaxDb	P0ACA7.
PRIDE	P0ACA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73925; AAC73925; b0838. BAA35541; BAA35541; BAA35541.
GeneID	12933830. 945469.
KEGG	ecj:Y75_p0811. eco:b0838.
PATRIC	32116879. VBIEscCol129921_0865.
Organism-specific databases
EchoBASE	EB3254.
EcoGene	EG13481. gstB.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125757.
OMA	VLWCAEE.
ProtClustDB	CLSK879786.
Enzyme and pathway databases
BioCyc	EcoCyc:G6438-MONOMER. ECOL316407:JW0822-MONOMER. MetaCyc:G6438-MONOMER.
Gene expression databases
Genevestigator	P0ACA7.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTB_ECOLI

Accession

Primary (citable) accession number: P0ACA7
Secondary accession number(s): P75805, Q9R7R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program