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P0ACA7

- GSTB_ECOLI

UniProt

P0ACA7 - GSTB_ECOLI

Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Kineticsi

    1. KM=5 mM for bromoacetate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity
    Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: EcoCyc
    2. identical protein binding Source: IntAct
    3. protein binding Source: IntAct

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase GstB (EC:2.5.1.18)
    Gene namesi
    Name:gstB
    Synonyms:yliJ
    Ordered Locus Names:b0838, JW0822
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13481. gstB.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are hypersensitive to bromoacetate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 208208Glutathione S-transferase GstBPRO_0000186018Add
    BLAST

    Proteomic databases

    PaxDbiP0ACA7.
    PRIDEiP0ACA7.

    Expressioni

    Gene expression databases

    GenevestigatoriP0ACA7.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1120568,EBI-1120568
    hisBP069874EBI-1120568,EBI-1126930

    Protein-protein interaction databases

    DIPiDIP-48220N.
    IntActiP0ACA7. 2 interactions.
    STRINGi511145.b0838.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 8383GST N-terminalAdd
    BLAST
    Domaini88 – 208121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125757.
    OMAiDIAFAPW.
    OrthoDBiEOG696BVR.
    PhylomeDBiP0ACA7.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACA7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN    50
    GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD 100
    WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK 150
    VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR 200
    KVVMIPVS 208
    Length:208
    Mass (Da):23,713
    Last modified:November 8, 2005 - v1
    Checksum:i8AD199E313B05094
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2.
    AP009048 Genomic DNA. Translation: BAA35541.1.
    RefSeqiNP_415359.4. NC_000913.3.
    YP_489111.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838.
    BAA35541; BAA35541; BAA35541.
    GeneIDi12933830.
    945469.
    KEGGiecj:Y75_p0811.
    eco:b0838.
    PATRICi32116879. VBIEscCol129921_0865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2 .
    AP009048 Genomic DNA. Translation: BAA35541.1 .
    RefSeqi NP_415359.4. NC_000913.3.
    YP_489111.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0ACA7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48220N.
    IntActi P0ACA7. 2 interactions.
    STRINGi 511145.b0838.

    Proteomic databases

    PaxDbi P0ACA7.
    PRIDEi P0ACA7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73925 ; AAC73925 ; b0838 .
    BAA35541 ; BAA35541 ; BAA35541 .
    GeneIDi 12933830.
    945469.
    KEGGi ecj:Y75_p0811.
    eco:b0838.
    PATRICi 32116879. VBIEscCol129921_0865.

    Organism-specific databases

    EchoBASEi EB3254.
    EcoGenei EG13481. gstB.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125757.
    OMAi DIAFAPW.
    OrthoDBi EOG696BVR.
    PhylomeDBi P0ACA7.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Miscellaneous databases

    PROi P0ACA7.

    Gene expression databases

    Genevestigatori P0ACA7.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate."
      Desai K.K., Miller B.G.
      Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

    Entry informationi

    Entry nameiGSTB_ECOLI
    AccessioniPrimary (citable) accession number: P0ACA7
    Secondary accession number(s): P75805, Q9R7R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3