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Protein

Glutathione S-transferase GstB

Gene

gstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=5 mM for bromoacetate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei12GlutathioneBy similarity1
    Binding sitei39GlutathioneBy similarity1
    Binding sitei53Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

    GO - Molecular functioni

    • arsenate reductase activity Source: EcoCyc
    • glutathione transferase activity Source: EcoCyc

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase GstB (EC:2.5.1.18)
    Gene namesi
    Name:gstB
    Synonyms:yliJ
    Ordered Locus Names:b0838, JW0822
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13481. gstB.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are hypersensitive to bromoacetate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001860181 – 208Glutathione S-transferase GstBAdd BLAST208

    Proteomic databases

    EPDiP0ACA7.
    PaxDbiP0ACA7.
    PRIDEiP0ACA7.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1120568,EBI-1120568
    hisBP069874EBI-1120568,EBI-1126930

    Protein-protein interaction databases

    BioGridi4262824. 11 interactors.
    DIPiDIP-48220N.
    IntActiP0ACA7. 2 interactors.
    STRINGi511145.b0838.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ACA7.
    SMRiP0ACA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 83GST N-terminalAdd BLAST83
    Domaini88 – 208GST C-terminalAdd BLAST121

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni67 – 68Glutathione bindingBy similarity2

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiENOG4108TGY. Bacteria.
    COG0625. LUCA.
    HOGENOMiHOG000125757.
    InParanoidiP0ACA7.
    KOiK00799.
    OMAiDIAFAPW.
    PhylomeDBiP0ACA7.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0ACA7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
    60 70 80 90 100
    GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
    110 120 130 140 150
    WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
    160 170 180 190 200
    VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR

    KVVMIPVS
    Length:208
    Mass (Da):23,713
    Last modified:November 8, 2005 - v1
    Checksum:i8AD199E313B05094
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2.
    AP009048 Genomic DNA. Translation: BAA35541.1.
    RefSeqiNP_415359.4. NC_000913.3.
    WP_001295292.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838.
    BAA35541; BAA35541; BAA35541.
    GeneIDi945469.
    KEGGiecj:JW0822.
    eco:b0838.
    PATRICi32116879. VBIEscCol129921_0865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC73925.2.
    AP009048 Genomic DNA. Translation: BAA35541.1.
    RefSeqiNP_415359.4. NC_000913.3.
    WP_001295292.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0ACA7.
    SMRiP0ACA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262824. 11 interactors.
    DIPiDIP-48220N.
    IntActiP0ACA7. 2 interactors.
    STRINGi511145.b0838.

    Proteomic databases

    EPDiP0ACA7.
    PaxDbiP0ACA7.
    PRIDEiP0ACA7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73925; AAC73925; b0838.
    BAA35541; BAA35541; BAA35541.
    GeneIDi945469.
    KEGGiecj:JW0822.
    eco:b0838.
    PATRICi32116879. VBIEscCol129921_0865.

    Organism-specific databases

    EchoBASEiEB3254.
    EcoGeneiEG13481. gstB.

    Phylogenomic databases

    eggNOGiENOG4108TGY. Bacteria.
    COG0625. LUCA.
    HOGENOMiHOG000125757.
    InParanoidiP0ACA7.
    KOiK00799.
    OMAiDIAFAPW.
    PhylomeDBiP0ACA7.

    Enzyme and pathway databases

    BioCyciEcoCyc:G6438-MONOMER.
    ECOL316407:JW0822-MONOMER.
    MetaCyc:G6438-MONOMER.

    Miscellaneous databases

    PROiP0ACA7.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGSTB_ECOLI
    AccessioniPrimary (citable) accession number: P0ACA7
    Secondary accession number(s): P75805, Q9R7R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: November 2, 2016
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.