SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0ACA7

- GSTB_ECOLI

UniProt

P0ACA7 - GSTB_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione S-transferase GstB
Gene
gstB, yliJ, b0838, JW0822
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=5 mM for bromoacetate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Glutathione By similarity
Binding sitei39 – 391Glutathione By similarity
Binding sitei53 – 531Glutathione; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: EcoCyc
  2. identical protein binding Source: IntAct
  3. protein binding Source: IntAct

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6438-MONOMER.
ECOL316407:JW0822-MONOMER.
MetaCyc:G6438-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase GstB (EC:2.5.1.18)
Gene namesi
Name:gstB
Synonyms:yliJ
Ordered Locus Names:b0838, JW0822
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13481. gstB.

Pathology & Biotechi

Disruption phenotypei

Mutants are hypersensitive to bromoacetate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Glutathione S-transferase GstB
PRO_0000186018Add
BLAST

Proteomic databases

PaxDbiP0ACA7.
PRIDEiP0ACA7.

Expressioni

Gene expression databases

GenevestigatoriP0ACA7.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1120568,EBI-1120568
hisBP069874EBI-1120568,EBI-1126930

Protein-protein interaction databases

DIPiDIP-48220N.
IntActiP0ACA7. 2 interactions.
STRINGi511145.b0838.

Structurei

3D structure databases

ProteinModelPortaliP0ACA7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8383GST N-terminal
Add
BLAST
Domaini88 – 208121GST C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 682Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125757.
OMAiDIAFAPW.
OrthoDBiEOG696BVR.
PhylomeDBiP0ACA7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0ACA7-1 [UniParc]FASTAAdd to Basket

« Hide

MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN    50
GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD 100
WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK 150
VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR 200
KVVMIPVS 208
Length:208
Mass (Da):23,713
Last modified:November 8, 2005 - v1
Checksum:i8AD199E313B05094
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73925.2.
AP009048 Genomic DNA. Translation: BAA35541.1.
RefSeqiNP_415359.4. NC_000913.3.
YP_489111.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73925; AAC73925; b0838.
BAA35541; BAA35541; BAA35541.
GeneIDi12933830.
945469.
KEGGiecj:Y75_p0811.
eco:b0838.
PATRICi32116879. VBIEscCol129921_0865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73925.2 .
AP009048 Genomic DNA. Translation: BAA35541.1 .
RefSeqi NP_415359.4. NC_000913.3.
YP_489111.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0ACA7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48220N.
IntActi P0ACA7. 2 interactions.
STRINGi 511145.b0838.

Proteomic databases

PaxDbi P0ACA7.
PRIDEi P0ACA7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73925 ; AAC73925 ; b0838 .
BAA35541 ; BAA35541 ; BAA35541 .
GeneIDi 12933830.
945469.
KEGGi ecj:Y75_p0811.
eco:b0838.
PATRICi 32116879. VBIEscCol129921_0865.

Organism-specific databases

EchoBASEi EB3254.
EcoGenei EG13481. gstB.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125757.
OMAi DIAFAPW.
OrthoDBi EOG696BVR.
PhylomeDBi P0ACA7.

Enzyme and pathway databases

BioCyci EcoCyc:G6438-MONOMER.
ECOL316407:JW0822-MONOMER.
MetaCyc:G6438-MONOMER.

Miscellaneous databases

PROi P0ACA7.

Gene expression databases

Genevestigatori P0ACA7.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate."
    Desai K.K., Miller B.G.
    Proc. Natl. Acad. Sci. U.S.A. 107:17968-17973(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE NAME.

Entry informationi

Entry nameiGSTB_ECOLI
AccessioniPrimary (citable) accession number: P0ACA7
Secondary accession number(s): P75805, Q9R7R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi