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Protein

Stringent starvation protein A

Gene

sspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme. It is synthesized predominantly when cells are exposed to amino acid starvation, at which time it accounts for over 50% of the total protein synthesized. It is involved in the transition from P1 early to P1 late gene expression. Rnk and SspA can functionally replace P.aeruginosa alginate regulatory gene algR2.

GO - Molecular functioni

GO - Biological processi

  • glutathione metabolic process Source: GO_Central
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • response to starvation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG10977-MONOMER.
ECOL316407:JW3198-MONOMER.

Protein family/group databases

TCDBi1.A.12.3.1. the intracellular chloride channel (clic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Stringent starvation protein A
Gene namesi
Name:sspA
Synonyms:pog, ssp
Ordered Locus Names:b3229, JW3198
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10977. sspA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 212211Stringent starvation protein APRO_0000185876Add
BLAST

Proteomic databases

EPDiP0ACA3.
PaxDbiP0ACA3.
PRIDEiP0ACA3.

2D gel databases

SWISS-2DPAGEP0ACA3.

Expressioni

Inductioni

By amino acid starvation.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
hrsAP547454EBI-558482,EBI-558542

Protein-protein interaction databases

BioGridi4262445. 10 interactions.
DIPiDIP-48104N.
IntActiP0ACA3. 13 interactions.
MINTiMINT-1228576.
STRINGi511145.b3229.

Structurei

3D structure databases

ProteinModelPortaliP0ACA3.
SMRiP0ACA3. Positions 4-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8779GST N-terminalAdd
BLAST
Domaini92 – 209118GST C-terminalAdd
BLAST

Sequence similaritiesi

Belongs to the GST superfamily. HSP26 family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG4107MKT. Bacteria.
COG0625. LUCA.
HOGENOMiHOG000255228.
InParanoidiP0ACA3.
KOiK03599.
OMAiMHRIQRD.
PhylomeDBiP0ACA3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ACA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAANKRSV MTLFSGPTDI YSHQVRIVLA EKGVSFEIEH VEKDNPPQDL
60 70 80 90 100
IDLNPNQSVP TLVDRELTLW ESRIIMEYLD ERFPHPPLMP VYPVARGESR
110 120 130 140 150
LYMHRIEKDW YTLMNTIING SASEADAARK QLREELLAIA PVFGQKPYFL
160 170 180 190 200
SDEFSLVDCY LAPLLWRLPQ LGIEFSGPGA KELKGYMTRV FERDSFLASL
210
TEAEREMRLG RS
Length:212
Mass (Da):24,305
Last modified:January 23, 2007 - v2
Checksum:iA0FFA98F7131B34F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05088 Genomic DNA. Translation: CAA28740.1.
U18997 Genomic DNA. Translation: AAA58031.1.
U00096 Genomic DNA. Translation: AAC76261.1.
AP009048 Genomic DNA. Translation: BAE77272.1.
PIRiA26422. RGECSS.
RefSeqiNP_417696.1. NC_000913.3.
WP_000257293.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76261; AAC76261; b3229.
BAE77272; BAE77272; BAE77272.
GeneIDi944744.
KEGGiecj:JW3198.
eco:b3229.
PATRICi32121882. VBIEscCol129921_3325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05088 Genomic DNA. Translation: CAA28740.1.
U18997 Genomic DNA. Translation: AAA58031.1.
U00096 Genomic DNA. Translation: AAC76261.1.
AP009048 Genomic DNA. Translation: BAE77272.1.
PIRiA26422. RGECSS.
RefSeqiNP_417696.1. NC_000913.3.
WP_000257293.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ACA3.
SMRiP0ACA3. Positions 4-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262445. 10 interactions.
DIPiDIP-48104N.
IntActiP0ACA3. 13 interactions.
MINTiMINT-1228576.
STRINGi511145.b3229.

Protein family/group databases

TCDBi1.A.12.3.1. the intracellular chloride channel (clic) family.

2D gel databases

SWISS-2DPAGEP0ACA3.

Proteomic databases

EPDiP0ACA3.
PaxDbiP0ACA3.
PRIDEiP0ACA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76261; AAC76261; b3229.
BAE77272; BAE77272; BAE77272.
GeneIDi944744.
KEGGiecj:JW3198.
eco:b3229.
PATRICi32121882. VBIEscCol129921_3325.

Organism-specific databases

EchoBASEiEB0970.
EcoGeneiEG10977. sspA.

Phylogenomic databases

eggNOGiENOG4107MKT. Bacteria.
COG0625. LUCA.
HOGENOMiHOG000255228.
InParanoidiP0ACA3.
KOiK03599.
OMAiMHRIQRD.
PhylomeDBiP0ACA3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10977-MONOMER.
ECOL316407:JW3198-MONOMER.

Miscellaneous databases

PROiP0ACA3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSPA_ECOLI
AccessioniPrimary (citable) accession number: P0ACA3
Secondary accession number(s): P05838, Q2M8Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.