P0ACA1 (YIBF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized GST-like protein YibF

Gene names

Name:	yibF
Ordered Locus Names:	b3592, JW3565

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glutathione (GSH) transferase homolog, that might be involved in selenium metabolism. Ref.6
Sequence similarities	Belongs to the GST superfamily. HSP26 family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 202

202

Uncharacterized GST-like protein YibF

PRO_0000185874

Regions

Domain

1 – 78

78

GST N-terminal

Domain

83 – 202

120

GST C-terminal

Region

62 – 63

2

Glutathione binding

Sites

Binding site

9

1

Glutathione

Binding site

49

1

Glutathione; via amide nitrogen and carbonyl oxygen

Experimental info

Sequence conflict

195

1

A → T Ref.1

Secondary structure

1

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202

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

10 – 21

12

Beta strand

27 – 30

4

Beta strand

35 – 37

3

Turn

41 – 43

3

Beta strand

51 – 53

3

Helix

63 – 72

10

Helix

84 – 112

29

Helix

115 – 117

3

Helix

120 – 142

23

Helix

153 – 168

16

Turn

172 – 177

6

Helix

179 – 189

11

Helix

192 – 195

4

Sequences

	Sequence		Length	Mass (Da)
	P0ACA1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BFEA058143049D67	FASTA	202	22,545
	10 20 30 40 50 60 MKLVGSYTSP FVRKLSILLL EKGITFEFIN ELPYNADNGV AQFNPLGKVP VLVTEEGECW 70 80 90 100 110 120 FDSPIIAEYI ELMNVAPAML PRDPLESLRV RKIEALADGI MDAGLVSVRE QARPAAQQSE 130 140 150 160 170 180 DELLRQREKI NRSLDVLEGY LVDGTLKTDT VNLATIAIAC AVGYLNFRRV APGWCVDRPH 190 200 LVKLVENLFS RESFARTEPP KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhs elements of Escherichia coli K-12: complex composites of shared and unique components that have different evolutionary histories." Zhao S., Sandt C.H., Feulner G., Vlazny D.A., Gray J.A., Hill C.W. J. Bacteriol. 175:2799-2808(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Correlation of Rhs elements with Escherichia coli population structure." Hill C.W., Feulner G., Brody M.S., Zhao S., Sadosky A.B., Sandt C.H. Genetics 141:15-24(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ECOR 32.
[3]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Conserved structural elements in glutathione transferase homologues encoded in the genome of Escherichia coli." Rife C.L., Parsons J.F., Xiao G., Gilliland G.L., Armstrong R.N. Proteins 53:777-782(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PUTATIVE FUNCTION IN SELENIUM BIOCHEMISTRY.
[7]	"Structural and functional genomics of YibF, a glutathione transferase homologue from Escherichia coli." Ladner J.E., Stournan N.V., Branch M.C., Harp J., Schaab M., Brown D.W., Armstrong R.N. Submitted (MAR-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L19044 Genomic DNA. Translation: AAC95064.1.
U16247 Genomic DNA. Translation: AAA56754.1.
U00039 Genomic DNA. Translation: AAB18569.1.
U00096 Genomic DNA. Translation: AAC76616.1.
AP009048 Genomic DNA. Translation: BAE77701.1.

PIR

S47813.

RefSeq

NP_418049.1. NC_000913.2.
YP_491842.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3R2Q	X-ray	1.05	A	1-202	[»]

ProteinModelPortal

P0ACA1.

SMR

P0ACA1. Positions 1-202.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0ACA1. 1 interaction.

STRING

511145.b3592.

Proteomic databases

PaxDb

P0ACA1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76616; AAC76616; b3592.
BAE77701; BAE77701; BAE77701.

GeneID

12932883.
948113.

KEGG

ecj:Y75_p3583.
eco:b3592.

PATRIC

32122662. VBIEscCol129921_3708.

Organism-specific databases

EchoBASE

EB1712.

EcoGene

EG11762. yibF.

Phylogenomic databases

eggNOG

COG0625.

HOGENOM

HOG000125756.

OMA

TFEFVNE.

ProtClustDB

PRK10357.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11762-MONOMER.
ECOL316407:JW3565-MONOMER.

Gene expression databases

Genevestigator

P0ACA1.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF00043. GST_C. 1 hit.
[Graphical view]

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YIBF_ECOLI

Accession

Primary (citable) accession number: P0ACA1
Secondary accession number(s): P32105, Q2M7Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program