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Protein

Succinate-acetate/proton symporter SatP

Gene

satP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of acetate and succinate. Transport is energetically dependent on the protonmotive force.1 Publication

Enzyme regulationi

Transport is inhibited by the protonophore CCCP.1 Publication

Kineticsi

  1. KM=1.24 mM for acetic acid1 Publication
  2. KM=1.18 mM for succinic acid1 Publication
  1. Vmax=8.72 nmol/min/mg enzyme with acetic acid as substrate1 Publication
  2. Vmax=10.05 nmol/min/mg enzyme with succinic acid as substrate1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

GO - Molecular functioni

  • acetate:proton symporter activity Source: EcoCyc

GO - Biological processi

  • acetate transmembrane transport Source: EcoCyc
  • succinate transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11512-MONOMER.
ECOL316407:JW0009-MONOMER.
MetaCyc:EG11512-MONOMER.

Protein family/group databases

TCDBi2.A.96.1.1. 2.a.96. the acetate uptake transporter (acetr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate-acetate/proton symporter SatP
Alternative name(s):
Succinate-acetate transporter protein
Gene namesi
Name:satP
Synonyms:yaaH
Ordered Locus Names:b0010, JW0009
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11512. satP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Topological domaini35 – 351PeriplasmicSequence analysis
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 637CytoplasmicSequence analysis
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST
Topological domaini85 – 9713PeriplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 1224CytoplasmicSequence analysis
Transmembranei123 – 14321HelicalSequence analysisAdd
BLAST
Topological domaini144 – 1485PeriplasmicSequence analysis
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 18819CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant shows a partial reduction in acetate uptake.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1311L → V: Acquires ability to transport lactic acid. 1 Publication
Mutagenesisi164 – 1641A → G: Acquires ability to transport lactic acid. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Succinate-acetate/proton symporter SatPPRO_0000135703Add
BLAST

Proteomic databases

PaxDbiP0AC98.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0010.

Structurei

3D structure databases

ProteinModelPortaliP0AC98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106HK8. Bacteria.
COG1584. LUCA.
HOGENOMiHOG000224166.
InParanoidiP0AC98.
KOiK07034.
OMAiAQFLGVF.
PhylomeDBiP0AC98.

Family and domain databases

InterProiIPR000791. Grp1/Fun34/SatP.
[Graphical view]
PfamiPF01184. Grp1_Fun34_YaaH. 1 hit.
[Graphical view]
PROSITEiPS01114. GPR1_FUN34_YAAH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNTKLANPA PLGLMGFGMT TILLNLHNVG YFALDGIILA MGIFYGGIAQ
60 70 80 90 100
IFAGLLEYKK GNTFGLTAFT SYGSFWLTLV AILLMPKLGL TDAPNAQFLG
110 120 130 140 150
VYLGLWGVFT LFMFFGTLKG ARVLQFVFFS LTVLFALLAI GNIAGNAAII
160 170 180
HFAGWIGLIC GASAIYLAMG EVLNEQFGRT VLPIGESH
Length:188
Mass (Da):20,071
Last modified:November 8, 2005 - v1
Checksum:i972101DD5949EBF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67700 Genomic DNA. Translation: CAA47931.1.
U00096 Genomic DNA. Translation: AAC73121.1.
AP009048 Genomic DNA. Translation: BAB96588.2.
PIRiE56688.
RefSeqiNP_414551.1. NC_000913.3.
WP_000528538.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73121; AAC73121; b0010.
BAB96588; BAB96588; BAB96588.
GeneIDi944792.
KEGGiecj:JW0009.
eco:b0010.
PATRICi32115115. VBIEscCol129921_0009.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67700 Genomic DNA. Translation: CAA47931.1.
U00096 Genomic DNA. Translation: AAC73121.1.
AP009048 Genomic DNA. Translation: BAB96588.2.
PIRiE56688.
RefSeqiNP_414551.1. NC_000913.3.
WP_000528538.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b0010.

Protein family/group databases

TCDBi2.A.96.1.1. 2.a.96. the acetate uptake transporter (acetr) family.

Proteomic databases

PaxDbiP0AC98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73121; AAC73121; b0010.
BAB96588; BAB96588; BAB96588.
GeneIDi944792.
KEGGiecj:JW0009.
eco:b0010.
PATRICi32115115. VBIEscCol129921_0009.

Organism-specific databases

EchoBASEiEB1474.
EcoGeneiEG11512. satP.

Phylogenomic databases

eggNOGiENOG4106HK8. Bacteria.
COG1584. LUCA.
HOGENOMiHOG000224166.
InParanoidiP0AC98.
KOiK07034.
OMAiAQFLGVF.
PhylomeDBiP0AC98.

Enzyme and pathway databases

BioCyciEcoCyc:EG11512-MONOMER.
ECOL316407:JW0009-MONOMER.
MetaCyc:EG11512-MONOMER.

Miscellaneous databases

PROiP0AC98.

Family and domain databases

InterProiIPR000791. Grp1/Fun34/SatP.
[Graphical view]
PfamiPF01184. Grp1_Fun34_YaaH. 1 hit.
[Graphical view]
PROSITEiPS01114. GPR1_FUN34_YAAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSATP_ECOLI
AccessioniPrimary (citable) accession number: P0AC98
Secondary accession number(s): P28695, Q8KMY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.