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Protein

Low-affinity gluconate transporter

Gene

gntU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the gluconate utilization system Gnt-I; low-affinity intake of gluconate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Gluconate utilization, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GNTU-MONOMER.
ECOL316407:JW5686-MONOMER.
MetaCyc:GNTU-MONOMER.
SABIO-RKP0AC96.

Protein family/group databases

TCDBi2.A.8.1.8. the gluconate:h(+) symporter (gntp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity gluconate transporter
Alternative name(s):
Gluconate permease
Gnt-I system
Gene namesi
Name:gntU
Ordered Locus Names:b4476, JW5686
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12631. gntU.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11CytoplasmicSequence analysis
Transmembranei2 – 2221HelicalSequence analysisAdd
BLAST
Topological domaini23 – 264PeriplasmicSequence analysis
Transmembranei27 – 4721HelicalSequence analysisAdd
BLAST
Topological domaini48 – 5811CytoplasmicSequence analysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence analysisAdd
BLAST
Topological domaini80 – 10930PeriplasmicSequence analysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence analysisAdd
BLAST
Topological domaini131 – 14212CytoplasmicSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Topological domaini164 – 17613PeriplasmicSequence analysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence analysisAdd
BLAST
Topological domaini198 – 22528CytoplasmicSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 26115PeriplasmicSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 29412CytoplasmicSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 33015PeriplasmicSequence analysisAdd
BLAST
Transmembranei331 – 35121HelicalSequence analysisAdd
BLAST
Topological domaini352 – 3521CytoplasmicSequence analysis
Transmembranei353 – 37321HelicalSequence analysisAdd
BLAST
Topological domaini374 – 38714PeriplasmicSequence analysisAdd
BLAST
Transmembranei388 – 40821HelicalSequence analysisAdd
BLAST
Topological domaini409 – 42416CytoplasmicSequence analysisAdd
BLAST
Transmembranei425 – 44521HelicalSequence analysisAdd
BLAST
Topological domaini446 – 4461PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Low-affinity gluconate transporterPRO_0000061935Add
BLAST

Proteomic databases

PaxDbiP0AC96.

Interactioni

Protein-protein interaction databases

BioGridi4261494. 5 interactions.
STRINGi511145.b4476.

Structurei

3D structure databases

ProteinModelPortaliP0AC96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GntP permease family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C6Z. Bacteria.
COG2610. LUCA.
HOGENOMiHOG000237114.
InParanoidiP0AC96.
KOiK06156.
OMAiELIVINC.
OrthoDBiEOG6B8XPC.
PhylomeDBiP0AC96.

Family and domain databases

InterProiIPR003474. Glcn_transporter.
[Graphical view]
PfamiPF02447. GntP_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF002746. Gluconate_transporter. 1 hit.
TIGRFAMsiTIGR00791. gntP. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTLVLTA VGSVLLLLFL VMKARMHAFL ALMVVSMGAG LFSGMPLDKI
60 70 80 90 100
AATMEKGMGG TLGFLAVVVA LGAMFGKILH ETGAVDQIAV KMLKSFGHSR
110 120 130 140 150
AHYAIGLAGL VCALPLFFEV AIVLLISVAF SMARHTGTNL VKLVIPLFAG
160 170 180 190 200
VAAAAAFLVP GPAPMLLASQ MNADFGWMIL IGLCAAIPGM IIAGPLWGNF
210 220 230 240 250
ISRYVELHIP DDISEPHLGE GKMPSFGFSL SLILLPLVLV GLKTIAARFV
260 270 280 290 300
PEGSTAYEWF EFIGHPFTAI LVACLVAIYG LAMRQGMPKD KVMEICGHAL
310 320 330 340 350
QPAGIILLVI GAGGVFKQVL VDSGVGPALG EALTGMGLPI AITCFVLAAA
360 370 380 390 400
VRIIQGSATV ACLTAVGLVM PVIEQLNYSG AQMAALSICI AGGSIVVSHV
410 420 430 440
NDAGFWLFGK FTGATEAETL KTWTMMETIL GTVGAIVGMI AFQLLS
Length:446
Mass (Da):46,416
Last modified:November 8, 2005 - v1
Checksum:i9BC51A785FF80A5F
GO

Sequence cautioni

The sequence AAA58233.1 differs from that shown. Reason: Frameshift at position 332. Curated
The sequence AAA58234.1 differs from that shown. Reason: Frameshift at position 332. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84362 Genomic DNA. Translation: BAA12326.1.
U18997 Genomic DNA. Translation: AAA58233.1. Frameshift.
U18997 Genomic DNA. Translation: AAA58234.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48183.1.
AP009048 Genomic DNA. Translation: BAE77857.1.
PIRiJC4989.
RefSeqiWP_000210111.1. NZ_LN832404.1.
YP_026221.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48183; AAT48183; b4476.
BAE77857; BAE77857; BAE77857.
GeneIDi2847760.
KEGGiecj:JW5686.
eco:b4476.
PATRICi32122310. VBIEscCol129921_3532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84362 Genomic DNA. Translation: BAA12326.1.
U18997 Genomic DNA. Translation: AAA58233.1. Frameshift.
U18997 Genomic DNA. Translation: AAA58234.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48183.1.
AP009048 Genomic DNA. Translation: BAE77857.1.
PIRiJC4989.
RefSeqiWP_000210111.1. NZ_LN832404.1.
YP_026221.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AC96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261494. 5 interactions.
STRINGi511145.b4476.

Protein family/group databases

TCDBi2.A.8.1.8. the gluconate:h(+) symporter (gntp) family.

Proteomic databases

PaxDbiP0AC96.

Protocols and materials databases

DNASUi2847760.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48183; AAT48183; b4476.
BAE77857; BAE77857; BAE77857.
GeneIDi2847760.
KEGGiecj:JW5686.
eco:b4476.
PATRICi32122310. VBIEscCol129921_3532.

Organism-specific databases

EchoBASEiEB2515.
EcoGeneiEG12631. gntU.

Phylogenomic databases

eggNOGiENOG4105C6Z. Bacteria.
COG2610. LUCA.
HOGENOMiHOG000237114.
InParanoidiP0AC96.
KOiK06156.
OMAiELIVINC.
OrthoDBiEOG6B8XPC.
PhylomeDBiP0AC96.

Enzyme and pathway databases

BioCyciEcoCyc:GNTU-MONOMER.
ECOL316407:JW5686-MONOMER.
MetaCyc:GNTU-MONOMER.
SABIO-RKP0AC96.

Miscellaneous databases

PROiP0AC96.

Family and domain databases

InterProiIPR003474. Glcn_transporter.
[Graphical view]
PfamiPF02447. GntP_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF002746. Gluconate_transporter. 1 hit.
TIGRFAMsiTIGR00791. gntP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli gntT and gntU genes and comparison of the products with their homologues."
    Yamada M., Kawai T., Izu H.
    Biosci. Biotechnol. Biochem. 60:1548-1550(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli."
    Izu H., Adachi O., Yamada M.
    J. Mol. Biol. 267:778-793(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: SEQUENCE REVISION.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism."
    Tong S., Porco A., Isturiz T., Conway T.
    J. Bacteriol. 178:3260-3269(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiGNTU_ECOLI
AccessioniPrimary (citable) accession number: P0AC96
Secondary accession number(s): P46858
, P76694, P76695, Q2M799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.