Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

High-affinity gluconate transporter

Gene

gntP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity gluconate transporter with fairly broad specificity, including low affinity for glucuronate, several disaccharides, and some hexoses, but not glucose.

Pathwayi: D-gluconate degradation

This protein is involved in the pathway D-gluconate degradation, which is part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the pathway D-gluconate degradation and in Carbohydrate acid metabolism.

GO - Molecular functioni

  • gluconate transmembrane transporter activity Source: EcoCyc
  • sugar:proton symporter activity Source: EcoCyc

GO - Biological processi

  • D-gluconate catabolic process Source: UniProtKB-UniPathway
  • gluconate transmembrane transport Source: EcoCyc
  • proton transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Gluconate utilization, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GNTP-MONOMER.
ECOL316407:JW4284-MONOMER.
MetaCyc:GNTP-MONOMER.
UniPathwayiUPA00792.

Protein family/group databases

TCDBi2.A.8.1.3. the gluconate:h(+) symporter (gntp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity gluconate transporter
Alternative name(s):
Gluconate permease 3
Gnt-III system
Gene namesi
Name:gntP
Synonyms:yjiB
Ordered Locus Names:b4321, JW4284
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12563. gntP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST
Topological domaini22 – 287PeriplasmicSequence analysis
Transmembranei29 – 4921HelicalSequence analysisAdd
BLAST
Topological domaini50 – 545CytoplasmicSequence analysis
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Topological domaini76 – 10328PeriplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 1262CytoplasmicSequence analysis
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Topological domaini148 – 17528PeriplasmicSequence analysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence analysisAdd
BLAST
Topological domaini197 – 22428CytoplasmicSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Topological domaini246 – 26015PeriplasmicSequence analysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Topological domaini282 – 29413CytoplasmicSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 32914PeriplasmicSequence analysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 3511CytoplasmicSequence analysis
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST
Topological domaini373 – 3742PeriplasmicSequence analysis
Transmembranei375 – 39521HelicalSequence analysisAdd
BLAST
Topological domaini396 – 42631CytoplasmicSequence analysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447High-affinity gluconate transporterPRO_0000061932Add
BLAST

Proteomic databases

PaxDbiP0AC94.

Interactioni

Protein-protein interaction databases

BioGridi4261000. 6 interactions.
STRINGi511145.b4321.

Structurei

3D structure databases

ProteinModelPortaliP0AC94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GntP permease family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107EFN. Bacteria.
COG2610. LUCA.
HOGENOMiHOG000237115.
InParanoidiP0AC94.
KOiK16321.
OMAiPINEIST.
OrthoDBiEOG6B8XPC.
PhylomeDBiP0AC94.

Family and domain databases

InterProiIPR003474. Glcn_transporter.
[Graphical view]
PfamiPF02447. GntP_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF002746. Gluconate_transporter. 1 hit.
TIGRFAMsiTIGR00791. gntP. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVLNILWVV FGIGLMLVLN LKFKINSMVA LLVAALSVGM LAGMDLMSLL
60 70 80 90 100
HTMKAGFGNT LGELAIIVVF GAVIGKLMVD SGAAHQIAHT LLARLGLRYV
110 120 130 140 150
QLSVIIIGLI FGLAMFYEVA FIMLAPLVIV IAAEAKIPFL KLAIPAVAAA
160 170 180 190 200
TTAHSLFPPQ PGPVALVNAY GADMGMVYIY GVLVTIPSVI CAGLILPKFL
210 220 230 240 250
GNLERPTPSF LKADQPVDMN NLPSFGVSIL VPLIPAIIMI STTIANIWLV
260 270 280 290 300
KDTPAWEVVN FIGSSPIAMF IAMVVAFVLF GTARGHDMQW VMNAFESAVK
310 320 330 340 350
SIAMVILIIG AGGVLKQTII DTGIGDTIGM LMSHGNISPY IMAWLITVLI
360 370 380 390 400
RLATGQGVVS AMTAAGIISA AILDPATGQL VGVNPALLVL ATAAGSNTLT
410 420 430 440
HINDASFWLF KGYFDLSVKD TLKTWGLLEL VNSVVGLIIV LIISMVA
Length:447
Mass (Da):47,138
Last modified:November 8, 2005 - v1
Checksum:i03B3AD16A61330E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631E → A in CAA62859 (PubMed:8550444).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91735 Genomic DNA. Translation: CAA62859.1.
U14003 Genomic DNA. Translation: AAA97217.1.
U00096 Genomic DNA. Translation: AAC77277.1.
AP009048 Genomic DNA. Translation: BAE78314.1.
PIRiS56546.
RefSeqiNP_418741.1. NC_000913.3.
WP_000558251.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77277; AAC77277; b4321.
BAE78314; BAE78314; BAE78314.
GeneIDi948848.
KEGGiecj:JW4284.
eco:b4321.
PATRICi32124238. VBIEscCol129921_4462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91735 Genomic DNA. Translation: CAA62859.1.
U14003 Genomic DNA. Translation: AAA97217.1.
U00096 Genomic DNA. Translation: AAC77277.1.
AP009048 Genomic DNA. Translation: BAE78314.1.
PIRiS56546.
RefSeqiNP_418741.1. NC_000913.3.
WP_000558251.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261000. 6 interactions.
STRINGi511145.b4321.

Protein family/group databases

TCDBi2.A.8.1.3. the gluconate:h(+) symporter (gntp) family.

Proteomic databases

PaxDbiP0AC94.

Protocols and materials databases

DNASUi948848.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77277; AAC77277; b4321.
BAE78314; BAE78314; BAE78314.
GeneIDi948848.
KEGGiecj:JW4284.
eco:b4321.
PATRICi32124238. VBIEscCol129921_4462.

Organism-specific databases

EchoBASEiEB2451.
EcoGeneiEG12563. gntP.

Phylogenomic databases

eggNOGiENOG4107EFN. Bacteria.
COG2610. LUCA.
HOGENOMiHOG000237115.
InParanoidiP0AC94.
KOiK16321.
OMAiPINEIST.
OrthoDBiEOG6B8XPC.
PhylomeDBiP0AC94.

Enzyme and pathway databases

UniPathwayiUPA00792.
BioCyciEcoCyc:GNTP-MONOMER.
ECOL316407:JW4284-MONOMER.
MetaCyc:GNTP-MONOMER.

Miscellaneous databases

PROiP0AC94.

Family and domain databases

InterProiIPR003474. Glcn_transporter.
[Graphical view]
PfamiPF02447. GntP_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF002746. Gluconate_transporter. 1 hit.
TIGRFAMsiTIGR00791. gntP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gntP gene of Escherichia coli involved in gluconate uptake."
    Klemm P., Tong S., Nielsen H., Conway T.
    J. Bacteriol. 178:61-67(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiGNTP_ECOLI
AccessioniPrimary (citable) accession number: P0AC94
Secondary accession number(s): P39373, Q2M5Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: January 20, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.