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Protein

Glycogen phosphorylase

Gene

glgP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

GO - Molecular functioni

  • glycogen phosphorylase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

  • carbohydrate catabolic process Source: EcoliWiki
  • glycogen catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOPHOSPHORYL-MONOMER.
ECOL316407:JW3391-MONOMER.
MetaCyc:GLYCOPHOSPHORYL-MONOMER.
RETL1328306-WGS:GSTH-3665-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase (EC:2.4.1.1)
Gene namesi
Name:glgP
Synonyms:glgY
Ordered Locus Names:b3428, JW3391
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10380. glgP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Glycogen phosphorylasePRO_0000188553Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei662 – 6621N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

EPDiP0AC86.
PaxDbiP0AC86.
PRIDEiP0AC86.

Interactioni

Protein-protein interaction databases

BioGridi4261263. 7 interactions.
DIPiDIP-47899N.
IntActiP0AC86. 1 interaction.
MINTiMINT-1307808.
STRINGi511145.b3428.

Structurei

3D structure databases

ProteinModelPortaliP0AC86.
SMRiP0AC86. Positions 13-812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiENOG4107QQN. Bacteria.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiP0AC86.
KOiK00688.
OMAiRMAWFSS.
PhylomeDBiP0AC86.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAPFTYSSP TLSVEALKHS IAYKLMFTIG KDPVVANKHE WLNATLFAVR
60 70 80 90 100
DRLVERWLRS NRAQLSQETR QVYYLSMEFL IGRTLSNAML SLGIYEDVQG
110 120 130 140 150
ALEAMGLNLE ELIDEENDPG LGNGGLGRLA ACFLDSLATL GLPGRGYGIR
160 170 180 190 200
YDYGMFKQNI VNGSQKESPD YWLEYGNPWE FKRHNTRYKV RFGGRIQQEG
210 220 230 240 250
KKTRWIETEE ILGVAYDQII PGYDTDATNT LRLWSAQASS EINLGKFNQG
260 270 280 290 300
DYFAAVEDKN HSENVSRVLY PDDSTYSGRE LRLRQEYFLV SSTIQDILSR
310 320 330 340 350
HYQLHKTYDN LADKIAIHLN DTHPVLSIPE MMRLLIDEHQ FSWDDAFEVC
360 370 380 390 400
CQVFSYTNHT LMSEALETWP VDMLGKILPR HLQIIFEIND YFLKTLQEQY
410 420 430 440 450
PNDTDLLGRA SIIDESNGRR VRMAWLAVVV SHKVNGVSEL HSNLMVQSLF
460 470 480 490 500
ADFAKIFPGR FTNVTNGVTP RRWLAVANPS LSAVLDEHLG RNWRTDLSLL
510 520 530 540 550
NELQQHCDFP MVNHAVHQAK LENKKRLAEY IAQQLNVVVN PKALFDVQIK
560 570 580 590 600
RIHEYKRQLM NVLHVITRYN RIKADPDAKW VPRVNIFGGK AASAYYMAKH
610 620 630 640 650
IIHLINDVAK VINNDPQIGD KLKVVFIPNY SVSLAQLIIP AADLSEQISL
660 670 680 690 700
AGTEASGTSN MKFALNGALT IGTLDGANVE MLDHVGADNI FIFGNTAEEV
710 720 730 740 750
EELRRQGYKP REYYEKDEEL HQVLTQIGSG VFSPEDPGRY RDLVDSLINF
760 770 780 790 800
GDHYQVLADY RSYVDCQDKV DELYELQEEW TAKAMLNIAN MGYFSSDRTI
810
KEYADHIWHI DPVRL
Length:815
Mass (Da):93,173
Last modified:November 8, 2005 - v1
Checksum:iB8046B91D8D7B468
GO

Sequence cautioni

The sequence CAA34807 differs from that shown. Reason: Frameshift at position 9. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 142SV → RL (PubMed:2645169).Curated
Sequence conflicti60 – 601S → P in AAA23875 (PubMed:2975249).Curated
Sequence conflicti94 – 952IY → TH in AAA23875 (PubMed:2975249).Curated
Sequence conflicti142 – 1421L → W in AAA23875 (PubMed:2975249).Curated
Sequence conflicti175 – 1751Y → S in CAA34807 (PubMed:2645169).Curated
Sequence conflicti182 – 1876Missing in AAA23874 (PubMed:3047129).Curated
Sequence conflicti191 – 1911R → V in CAA34807 (PubMed:2645169).Curated
Sequence conflicti248 – 2481N → D in AAA23875 (PubMed:2975249).Curated
Sequence conflicti280 – 2812EL → DV in AAA23874 (PubMed:3047129).Curated
Sequence conflicti280 – 2812EL → DV in AAA58226 (PubMed:3047129).Curated
Sequence conflicti280 – 2801E → G in AAA23875 (PubMed:2975249).Curated
Sequence conflicti324 – 3241P → S in AAA23875 (PubMed:2975249).Curated
Sequence conflicti377 – 3771I → S in AAA23875 (PubMed:2975249).Curated
Sequence conflicti450 – 4501F → L in AAA23874 (PubMed:3047129).Curated
Sequence conflicti487 – 4882EH → GT in CAA34807 (PubMed:2645169).Curated
Sequence conflicti520 – 5212KL → NV in CAA34807 (PubMed:2645169).Curated
Sequence conflicti729 – 7291S → T in AAA23874 (PubMed:3047129).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16931 Genomic DNA. Translation: CAA34807.1. Frameshift.
J03966 Genomic DNA. Translation: AAA23874.1.
U18997 Genomic DNA. Translation: AAA58226.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC76453.1.
AP009048 Genomic DNA. Translation: BAE77864.1.
M22368 Genomic DNA. Translation: AAA23875.1.
D00425 Genomic DNA. Translation: BAA00329.1.
J02616 Genomic DNA. Translation: AAA23871.1.
PIRiG65138. PHECGG.
RefSeqiNP_417886.1. NC_000913.3.
WP_000993449.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76453; AAC76453; b3428.
BAE77864; BAE77864; BAE77864.
GeneIDi947931.
KEGGiecj:JW3391.
eco:b3428.
PATRICi32122296. VBIEscCol129921_3525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16931 Genomic DNA. Translation: CAA34807.1. Frameshift.
J03966 Genomic DNA. Translation: AAA23874.1.
U18997 Genomic DNA. Translation: AAA58226.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC76453.1.
AP009048 Genomic DNA. Translation: BAE77864.1.
M22368 Genomic DNA. Translation: AAA23875.1.
D00425 Genomic DNA. Translation: BAA00329.1.
J02616 Genomic DNA. Translation: AAA23871.1.
PIRiG65138. PHECGG.
RefSeqiNP_417886.1. NC_000913.3.
WP_000993449.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC86.
SMRiP0AC86. Positions 13-812.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261263. 7 interactions.
DIPiDIP-47899N.
IntActiP0AC86. 1 interaction.
MINTiMINT-1307808.
STRINGi511145.b3428.

Proteomic databases

EPDiP0AC86.
PaxDbiP0AC86.
PRIDEiP0AC86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76453; AAC76453; b3428.
BAE77864; BAE77864; BAE77864.
GeneIDi947931.
KEGGiecj:JW3391.
eco:b3428.
PATRICi32122296. VBIEscCol129921_3525.

Organism-specific databases

EchoBASEiEB0375.
EcoGeneiEG10380. glgP.

Phylogenomic databases

eggNOGiENOG4107QQN. Bacteria.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiP0AC86.
KOiK00688.
OMAiRMAWFSS.
PhylomeDBiP0AC86.

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOPHOSPHORYL-MONOMER.
ECOL316407:JW3391-MONOMER.
MetaCyc:GLYCOPHOSPHORYL-MONOMER.
RETL1328306-WGS:GSTH-3665-MONOMER.

Miscellaneous databases

PROiP0AC86.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHSG_ECOLI
AccessioniPrimary (citable) accession number: P0AC86
Secondary accession number(s): P13031, Q2M792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.