Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycogen phosphorylase

Gene

glgP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

GO - Molecular functioni

  • glycogen phosphorylase activity Source: EcoCyc
  • linear malto-oligosaccharide phosphorylase activity Source: UniProtKB-EC
  • pyridoxal phosphate binding Source: EcoCyc
  • SHG alpha-glucan phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate catabolic process Source: EcoliWiki
  • glycogen catabolic process Source: EcoCyc

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GLYCOPHOSPHORYL-MONOMER
MetaCyc:GLYCOPHOSPHORYL-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase (EC:2.4.1.1)
Gene namesi
Name:glgP
Synonyms:glgY
Ordered Locus Names:b3428, JW3391
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10380 glgP

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001885531 – 815Glycogen phosphorylaseAdd BLAST815

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei662N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP0AC86
PaxDbiP0AC86
PRIDEiP0AC86

Interactioni

Protein-protein interaction databases

BioGridi4261263, 32 interactors
DIPiDIP-47899N
IntActiP0AC86, 2 interactors
STRINGi316385.ECDH10B_3602

Structurei

3D structure databases

ProteinModelPortaliP0AC86
SMRiP0AC86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiENOG4107QQN Bacteria
COG0058 LUCA
HOGENOMiHOG000278444
InParanoidiP0AC86
KOiK00688
OMAiWLEMSIN
PhylomeDBiP0AC86

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAPFTYSSP TLSVEALKHS IAYKLMFTIG KDPVVANKHE WLNATLFAVR
60 70 80 90 100
DRLVERWLRS NRAQLSQETR QVYYLSMEFL IGRTLSNAML SLGIYEDVQG
110 120 130 140 150
ALEAMGLNLE ELIDEENDPG LGNGGLGRLA ACFLDSLATL GLPGRGYGIR
160 170 180 190 200
YDYGMFKQNI VNGSQKESPD YWLEYGNPWE FKRHNTRYKV RFGGRIQQEG
210 220 230 240 250
KKTRWIETEE ILGVAYDQII PGYDTDATNT LRLWSAQASS EINLGKFNQG
260 270 280 290 300
DYFAAVEDKN HSENVSRVLY PDDSTYSGRE LRLRQEYFLV SSTIQDILSR
310 320 330 340 350
HYQLHKTYDN LADKIAIHLN DTHPVLSIPE MMRLLIDEHQ FSWDDAFEVC
360 370 380 390 400
CQVFSYTNHT LMSEALETWP VDMLGKILPR HLQIIFEIND YFLKTLQEQY
410 420 430 440 450
PNDTDLLGRA SIIDESNGRR VRMAWLAVVV SHKVNGVSEL HSNLMVQSLF
460 470 480 490 500
ADFAKIFPGR FTNVTNGVTP RRWLAVANPS LSAVLDEHLG RNWRTDLSLL
510 520 530 540 550
NELQQHCDFP MVNHAVHQAK LENKKRLAEY IAQQLNVVVN PKALFDVQIK
560 570 580 590 600
RIHEYKRQLM NVLHVITRYN RIKADPDAKW VPRVNIFGGK AASAYYMAKH
610 620 630 640 650
IIHLINDVAK VINNDPQIGD KLKVVFIPNY SVSLAQLIIP AADLSEQISL
660 670 680 690 700
AGTEASGTSN MKFALNGALT IGTLDGANVE MLDHVGADNI FIFGNTAEEV
710 720 730 740 750
EELRRQGYKP REYYEKDEEL HQVLTQIGSG VFSPEDPGRY RDLVDSLINF
760 770 780 790 800
GDHYQVLADY RSYVDCQDKV DELYELQEEW TAKAMLNIAN MGYFSSDRTI
810
KEYADHIWHI DPVRL
Length:815
Mass (Da):93,173
Last modified:November 8, 2005 - v1
Checksum:iB8046B91D8D7B468
GO

Sequence cautioni

The sequence CAA34807 differs from that shown. Reason: Frameshift at position 9.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13 – 14SV → RL (PubMed:2645169).Curated2
Sequence conflicti60S → P in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti94 – 95IY → TH in AAA23875 (PubMed:2975249).Curated2
Sequence conflicti142L → W in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti175Y → S in CAA34807 (PubMed:2645169).Curated1
Sequence conflicti182 – 187Missing in AAA23874 (PubMed:3047129).Curated6
Sequence conflicti191R → V in CAA34807 (PubMed:2645169).Curated1
Sequence conflicti248N → D in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti280 – 281EL → DV in AAA23874 (PubMed:3047129).Curated2
Sequence conflicti280 – 281EL → DV in AAA58226 (PubMed:3047129).Curated2
Sequence conflicti280E → G in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti324P → S in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti377I → S in AAA23875 (PubMed:2975249).Curated1
Sequence conflicti450F → L in AAA23874 (PubMed:3047129).Curated1
Sequence conflicti487 – 488EH → GT in CAA34807 (PubMed:2645169).Curated2
Sequence conflicti520 – 521KL → NV in CAA34807 (PubMed:2645169).Curated2
Sequence conflicti729S → T in AAA23874 (PubMed:3047129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16931 Genomic DNA Translation: CAA34807.1 Frameshift.
J03966 Genomic DNA Translation: AAA23874.1
U18997 Genomic DNA Translation: AAA58226.1 Sequence problems.
U00096 Genomic DNA Translation: AAC76453.1
AP009048 Genomic DNA Translation: BAE77864.1
M22368 Genomic DNA Translation: AAA23875.1
D00425 Genomic DNA Translation: BAA00329.1
J02616 Genomic DNA Translation: AAA23871.1
PIRiG65138 PHECGG
RefSeqiNP_417886.1, NC_000913.3
WP_000993449.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76453; AAC76453; b3428
BAE77864; BAE77864; BAE77864
GeneIDi947931
KEGGiecj:JW3391
eco:b3428
PATRICifig|1411691.4.peg.3300

Similar proteinsi

Entry informationi

Entry nameiPHSG_ECOLI
AccessioniPrimary (citable) accession number: P0AC86
Secondary accession number(s): P13031, Q2M792
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health