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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Kineticsi

  1. KM=184 µM for S-D-lactoyl-glutathione
  1. Vmax=122 µmol/min/mg enzyme

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (gloA)
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Zinc 1By similarity
Metal bindingi55 – 551Zinc 1By similarity
Metal bindingi57 – 571Zinc 2By similarity
Metal bindingi58 – 581Zinc 2By similarity
Metal bindingi110 – 1101Zinc 1By similarity
Metal bindingi127 – 1271Zinc 1By similarity
Metal bindingi127 – 1271Zinc 2By similarity
Metal bindingi165 – 1651Zinc 2By similarity

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: EcoCyc
  • response to heat Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GLYOXII-MONOMER.
ECOL316407:JW0202-MONOMER.
MetaCyc:GLYOXII-MONOMER.
BRENDAi3.1.2.6. 2026.
SABIO-RKP0AC84.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:gloB
Synonyms:yafR
Ordered Locus Names:b0212, JW0202
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13330. gloB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Hydroxyacylglutathione hydrolasePRO_0000192351Add
BLAST

Proteomic databases

EPDiP0AC84.
PaxDbiP0AC84.
PRIDEiP0AC84.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262005. 16 interactions.
DIPiDIP-48248N.
IntActiP0AC84. 1 interaction.
MINTiMINT-1295781.
STRINGi511145.b0212.

Structurei

3D structure databases

ProteinModelPortaliP0AC84.
SMRiP0AC84. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiP0AC84.
KOiK01069.
OMAiDNYIFLL.
PhylomeDBiP0AC84.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLNSIPAFD DNYIWVLNDE AGRCLIVDPG DAEPVLNAIA ANNWQPEAIF
60 70 80 90 100
LTHHHHDHVG GVKELVEKFP QIVVYGPQET QDKGTTQVVK DGETAFVLGH
110 120 130 140 150
EFSVIATPGH TLGHICYFSK PYLFCGDTLF SGGCGRLFEG TASQMYQSLK
160 170 180 190 200
KLSALPDDTL VCCAHEYTLS NMKFALSILP HDLSINDYYR KVKELRAKNQ
210 220 230 240 250
ITLPVILKNE RQINVFLRTE DIDLINVINE ETLLQQPEER FAWLRSKKDR

F
Length:251
Mass (Da):28,434
Last modified:November 8, 2005 - v1
Checksum:iD59948B6E12809F5
GO

Mass spectrometryi

Molecular mass is 28432 Da from positions 1 - 251. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08634.1.
U00096 Genomic DNA. Translation: AAC73317.1.
AP009048 Genomic DNA. Translation: BAA77883.1.
PIRiF64745.
RefSeqiNP_414748.1. NC_000913.3.
WP_001052715.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73317; AAC73317; b0212.
BAA77883; BAA77883; BAA77883.
GeneIDi944902.
KEGGiecj:JW0202.
eco:b0212.
PATRICi32115537. VBIEscCol129921_0214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08634.1.
U00096 Genomic DNA. Translation: AAC73317.1.
AP009048 Genomic DNA. Translation: BAA77883.1.
PIRiF64745.
RefSeqiNP_414748.1. NC_000913.3.
WP_001052715.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC84.
SMRiP0AC84. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262005. 16 interactions.
DIPiDIP-48248N.
IntActiP0AC84. 1 interaction.
MINTiMINT-1295781.
STRINGi511145.b0212.

Proteomic databases

EPDiP0AC84.
PaxDbiP0AC84.
PRIDEiP0AC84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73317; AAC73317; b0212.
BAA77883; BAA77883; BAA77883.
GeneIDi944902.
KEGGiecj:JW0202.
eco:b0212.
PATRICi32115537. VBIEscCol129921_0214.

Organism-specific databases

EchoBASEiEB3114.
EcoGeneiEG13330. gloB.

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiP0AC84.
KOiK01069.
OMAiDNYIFLL.
PhylomeDBiP0AC84.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciEcoCyc:GLYOXII-MONOMER.
ECOL316407:JW0202-MONOMER.
MetaCyc:GLYOXII-MONOMER.
BRENDAi3.1.2.6. 2026.
SABIO-RKP0AC84.

Miscellaneous databases

PROiP0AC84.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_ECOLI
AccessioniPrimary (citable) accession number: P0AC84
Secondary accession number(s): Q47677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mn2+ and Co2+ can substitute for zinc in reconstitution experiments.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.