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Protein

Hydroxyacylglutathione hydrolase GloB

Gene

gloB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Type II glyoxalase that catalyzes the hydrolysis of (R)-S-lactoylglutathione to (R)-lactate and glutathione (PubMed:25670698, PubMed:17196158). Is more efficient than the isozyme GloC, and plays a major contribution to methylglyoxal (MG) detoxification in E.coli (PubMed:25670698). The two isoenzymes have additive effects and ensure maximal MG degradation (PubMed:25670698).2 Publications

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.2 Publications
(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione.2 Publications

Cofactori

Zn2+1 Publication1 PublicationNote: Binds 2 Zn2+ ions per subunit. Mn2+ and Co2+ can substitute for zinc in reconstitution experiments.1 Publication

Enzyme regulationi

Is inhibited by Cu2+.1 Publication

Kineticsi

kcat is 53 sec(-1) for the hydrolysis of (R)-S-lactoylglutathione (PubMed:17196158). kcat is 15.6 sec(-1) for the hydrolysis of (R)-S-lactoylglutathione (PubMed:25670698).2 Publications
  1. KM=184 µM for (R)-S-lactoylglutathione1 Publication
  2. KM=0.5 mM for (R)-S-lactoylglutathione1 Publication
  1. Vmax=112 µmol/min/mg enzyme for the hydrolysis of (R)-S-lactoylglutathione1 Publication

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (gloA)
  2. Hydroxyacylglutathione hydrolase GloB (gloB), Hydroxyacylglutathione hydrolase GloC (gloC)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Zinc 1; via tele nitrogenBy similarity1
Metal bindingi55Zinc 1; via pros nitrogenBy similarity1
Metal bindingi57Zinc 2By similarity1
Metal bindingi58Zinc 2; via tele nitrogenBy similarity1
Metal bindingi110Zinc 1; via tele nitrogenBy similarity1
Metal bindingi127Zinc 1By similarity1
Metal bindingi127Zinc 2By similarity1
Metal bindingi165Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: EcoCyc
  • response to heat Source: EcoCyc
  • response to toxic substance Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processDetoxification
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GLYOXII-MONOMER.
MetaCyc:GLYOXII-MONOMER.
BRENDAi3.1.2.6. 2026.
SABIO-RKiP0AC84.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase GloB2 Publications (EC:3.1.2.62 Publications)
Alternative name(s):
Glyoxalase II2 Publications
Short name:
Glx II2 Publications
Gene namesi
Name:gloB1 Publication
Synonyms:yafR
Ordered Locus Names:b0212, JW0202
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13330. gloB.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show decreased methylglyoxal tolerance. A double deletion mutant lacking both gloB and gloC exhibits almost no resistance to exogenously supplied methylglyoxal, and is unable to grow at MG concentrations as low as 0.1 mM.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001923511 – 251Hydroxyacylglutathione hydrolase GloBAdd BLAST251

Proteomic databases

EPDiP0AC84.
PaxDbiP0AC84.
PRIDEiP0AC84.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262005. 16 interactors.
DIPiDIP-48248N.
IntActiP0AC84. 1 interactor.
MINTiMINT-1295781.
STRINGi511145.b0212.

Structurei

3D structure databases

ProteinModelPortaliP0AC84.
SMRiP0AC84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 138Substrate bindingBy similarity3
Regioni165 – 167Substrate bindingBy similarity3
Regioni245 – 248Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiP0AC84.
KOiK01069.
OMAiDNYIFLL.
PhylomeDBiP0AC84.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AC84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLNSIPAFD DNYIWVLNDE AGRCLIVDPG DAEPVLNAIA ANNWQPEAIF
60 70 80 90 100
LTHHHHDHVG GVKELVEKFP QIVVYGPQET QDKGTTQVVK DGETAFVLGH
110 120 130 140 150
EFSVIATPGH TLGHICYFSK PYLFCGDTLF SGGCGRLFEG TASQMYQSLK
160 170 180 190 200
KLSALPDDTL VCCAHEYTLS NMKFALSILP HDLSINDYYR KVKELRAKNQ
210 220 230 240 250
ITLPVILKNE RQINVFLRTE DIDLINVINE ETLLQQPEER FAWLRSKKDR

F
Length:251
Mass (Da):28,434
Last modified:November 8, 2005 - v1
Checksum:iD59948B6E12809F5
GO

Mass spectrometryi

Molecular mass is 28432 Da from positions 1 - 251. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08634.1.
U00096 Genomic DNA. Translation: AAC73317.1.
AP009048 Genomic DNA. Translation: BAA77883.1.
PIRiF64745.
RefSeqiNP_414748.1. NC_000913.3.
WP_001052715.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73317; AAC73317; b0212.
BAA77883; BAA77883; BAA77883.
GeneIDi944902.
KEGGiecj:JW0202.
eco:b0212.
PATRICi32115537. VBIEscCol129921_0214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08634.1.
U00096 Genomic DNA. Translation: AAC73317.1.
AP009048 Genomic DNA. Translation: BAA77883.1.
PIRiF64745.
RefSeqiNP_414748.1. NC_000913.3.
WP_001052715.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC84.
SMRiP0AC84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262005. 16 interactors.
DIPiDIP-48248N.
IntActiP0AC84. 1 interactor.
MINTiMINT-1295781.
STRINGi511145.b0212.

Proteomic databases

EPDiP0AC84.
PaxDbiP0AC84.
PRIDEiP0AC84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73317; AAC73317; b0212.
BAA77883; BAA77883; BAA77883.
GeneIDi944902.
KEGGiecj:JW0202.
eco:b0212.
PATRICi32115537. VBIEscCol129921_0214.

Organism-specific databases

EchoBASEiEB3114.
EcoGeneiEG13330. gloB.

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiP0AC84.
KOiK01069.
OMAiDNYIFLL.
PhylomeDBiP0AC84.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciEcoCyc:GLYOXII-MONOMER.
MetaCyc:GLYOXII-MONOMER.
BRENDAi3.1.2.6. 2026.
SABIO-RKiP0AC84.

Miscellaneous databases

PROiPR:P0AC84.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_ECOLI
AccessioniPrimary (citable) accession number: P0AC84
Secondary accession number(s): Q47677
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: February 15, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.