P0AC84 (GLO2_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 66.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Hydroxyacylglutathione hydrolase EC=3.1.2.6 Alternative name(s): Glyoxalase II Short name=Glx II | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 251 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid. HAMAP-Rule MF_01374 |
| Catalytic activity | S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP-Rule MF_01374 |
| Cofactor | Binds 2 zinc ions per subunit. Ref.5 |
| Pathway | Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374 |
| Subunit structure | Monomer. Ref.5 |
| Miscellaneous | Mn2+ and Co2+ can substitute for zinc in reconstitution experiments. HAMAP-Rule MF_01374 |
| Sequence similarities | Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. |
| Biophysicochemical properties | Kinetic parameters: KM=184 µM for S-D-lactoyl-glutathione Vmax=122 µmol/min/mg enzyme |
| Mass spectrometry | Molecular mass is 28432 Da from positions 1 - 251. Determined by ESI. Ref.5 |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro methylglyoxal catabolic process to D-lactateInferred from direct assay Ref.5. Source: EcoCyc |
| Molecular_function | hydroxyacylglutathione hydrolase activity Inferred from direct assay Ref.5. Source: EcoCyc zinc ion bindingInferred from direct assay Ref.5. Source: EcoCyc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 251 | 251 | Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374 | PRO_0000192351 | |||||
Sites | |||||||||
| Metal binding | 53 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 55 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 57 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 58 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 110 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 127 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 127 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 165 | 1 | Zinc 2 By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U70214 Genomic DNA. Translation: AAB08634.1. U00096 Genomic DNA. Translation: AAC73317.1. AP009048 Genomic DNA. Translation: BAA77883.1. |
| PIR | F64745. |
| RefSeq | NP_414748.1. NC_000913.2. YP_488509.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | P0AC84. |
| SMR | P0AC84. Positions 1-251. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-48248N. |
| IntAct | P0AC84. 1 interaction. |
| MINT | MINT-1295781. |
| STRING | 511145.b0212. |
Proteomic databases | |
| PaxDb | P0AC84. |
| PRIDE | P0AC84. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC73317; AAC73317; b0212. BAA77883; BAA77883; BAA77883. |
| GeneID | 12933011. 944902. |
| KEGG | ecj:Y75_p0203. eco:b0212. |
| PATRIC | 32115537. VBIEscCol129921_0214. |
Organism-specific databases | |
| EchoBASE | EB3114. |
| EcoGene | EG13330. gloB. |
Phylogenomic databases | |
| eggNOG | COG0491. |
| HOGENOM | HOG000058041. |
| KO | K01069. |
| OMA | PFMRVHE. |
| ProtClustDB | PRK10241. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:GLYOXII-MONOMER. ECOL316407:JW0202-MONOMER. MetaCyc:GLYOXII-MONOMER. |
| SABIO-RK | P0AC84. |
| UniPathway | UPA00619; UER00676. |
Gene expression databases | |
| Genevestigator | P0AC84. |
Family and domain databases | |
| HAMAP | MF_01374. Glyoxalase_2. |
| InterPro | IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view] |
| PANTHER | PTHR11935:SF7. PTHR11935:SF7. 1 hit. |
| Pfam | PF00753. Lactamase_B. 1 hit. [Graphical view] |
| PIRSF | PIRSF005457. Glx. 1 hit. |
| SMART | SM00849. Lactamase_B. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR03413. GSH_gloB. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | GLO2_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0AC84 Secondary accession number(s): Q47677 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
