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Protein

Lactoylglutathione lyase

Gene

gloA

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.By similarity

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit. In the homodimer, two nickel ions are bound between subunits.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 1 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (RK59_001765), Lactoylglutathione lyase (gloA), Lactoylglutathione lyase (CEG98_04650)
  2. Hydroxyacylglutathione hydrolase (gloB), Hydroxyacylglutathione hydrolase (gloB), Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Nickel; via tele nitrogen; shared with dimeric partnerBy similarity1
Binding sitei9Substrate; shared with dimeric partnerBy similarity1
Metal bindingi56Nickel; shared with dimeric partnerBy similarity1
Binding sitei60Substrate; shared with dimeric partnerBy similarity1
Metal bindingi74Nickel; via tele nitrogenBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei122Proton donor/acceptorBy similarity1
Metal bindingi122NickelBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionLyase
LigandMetal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:gloA
Ordered Locus Names:SF1678, S1810
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001680921 – 135Lactoylglutathione lyaseAdd BLAST135

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0AC83
SMRiP0AC83
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 126VOCPROSITE-ProRule annotationAdd BLAST125

Sequence similaritiesi

Belongs to the glyoxalase I family.Curated

Phylogenomic databases

eggNOGiENOG4108UYE Bacteria
COG0346 LUCA
HOGENOMiHOG000232011
KOiK01759
OMAiNWGTESY

Family and domain databases

Gene3Di3.10.180.10, 1 hit
InterProiView protein in InterPro
IPR029068 Glyas_Bleomycin-R_OHBP_Dase
IPR004360 Glyas_Fos-R_dOase_dom
IPR004361 Glyoxalase_1
IPR018146 Glyoxalase_1_CS
IPR037523 VOC
PfamiView protein in Pfam
PF00903 Glyoxalase, 1 hit
SUPFAMiSSF54593 SSF54593, 1 hit
TIGRFAMsiTIGR00068 glyox_I, 1 hit
PROSITEiView protein in PROSITE
PS00934 GLYOXALASE_I_1, 1 hit
PS00935 GLYOXALASE_I_2, 1 hit
PS51819 VOC, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLHTMLRV GDLQRSIDFY TKVLGMKLLR TSENPEYKYS LAFVGYGPET
60 70 80 90 100
EEAVIELTYN WGVDKYELGT AYGHIALSVD NAAEACEKIR QNGGNVTREA
110 120 130
GPVKGGTTVI AFVEDPDGYK IELIEEKDAG RGLGN
Length:135
Mass (Da):14,920
Last modified:November 8, 2005 - v1
Checksum:iA53FC5412B9A6CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN43259.1
AE014073 Genomic DNA Translation: AAP17147.1
RefSeqiNP_707552.1, NC_004337.2
WP_001237796.1, NZ_NIYV01000062.1

Genome annotation databases

EnsemblBacteriaiAAN43259; AAN43259; SF1678
AAP17147; AAP17147; S1810
GeneIDi1024857
KEGGisfl:SF1678
sfx:S1810
PATRICifig|198214.7.peg.1977

Similar proteinsi

Entry informationi

Entry nameiLGUL_SHIFL
AccessioniPrimary (citable) accession number: P0AC83
Secondary accession number(s): P77036, Q59384
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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