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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.By similarity

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathwayi: KDO(2)-lipid A biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  2. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  3. Lipid A biosynthesis lauroyltransferase (lpxL)
  4. Lipid A biosynthesis myristoyltransferase (lpxM), Lipid A biosynthesis myristoyltransferase (msbB), Lipid A biosynthesis myristoyltransferase (lpxM), Lipid A biosynthesis myristoyltransferase (lpxM)
This subpathway is part of the pathway KDO(2)-lipid A biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Proton acceptorBy similarity1
Sitei130Transition state stabilizerBy similarity1
Sitei208Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00360; UER00483
UPA00360; UER00484
UPA00958

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.12, EC:2.4.99.13)
Short name:
Kdo transferase
Alternative name(s):
Bifunctional Kdo transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:kdtA
Ordered Locus Names:Z5057, ECs4508
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23Helical; Signal-anchorSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802871 – 4253-deoxy-D-manno-octulosonic acid transferaseAdd BLAST425

Proteomic databases

PRIDEiP0AC77

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5057

Structurei

3D structure databases

ProteinModelPortaliP0AC77
SMRiP0AC77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 269CMP-Kdo bindingBy similarity2
Regioni309 – 311CMP-Kdo bindingBy similarity3
Regioni335 – 338CMP-Kdo bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8A Bacteria
COG1519 LUCA
HOGENOMiHOG000257156
KOiK02527
OMAiFIKYEFW

Family and domain databases

Gene3Di3.40.50.11720, 1 hit
InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR007507 Glycos_transf_N
IPR038107 Glycos_transf_N_sf
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF04413 Glycos_transf_N, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELLYTALL YLIQPLIWIR LWVRGRKAPA YRKRWGERYG FYRHPLKPGG
60 70 80 90 100
IMLHSVSVGE TLAAIPLVRA LRHRYPDLPI TVTTMTPTGS ERVQSAFGKD
110 120 130 140 150
VQHVYLPYDL PDALNRFLNK VDPKLVLIME TELWPNLIAA LHKRKIPLVI
160 170 180 190 200
ANARLSARSA AGYAKLGKFV RRLLRRITLI AAQNEEDGAR FVALGAKNNQ
210 220 230 240 250
VTVTGSLKFD ISVTPQLAAK AVTLRRQWAP HRPVWIATST HEGEESVVIA
260 270 280 290 300
AHQALLQQFP NLLLILVPRH PERFPDAINL VRQAGLSYIT RSSGEVPSTS
310 320 330 340 350
TQVVVGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP
360 370 380 390 400
HTFNFKDICA RLEQASGLIT VTDATTLAKE VSSLLTDADY RSFYGRHAVE
410 420
VLYQNQGALQ RLLQLLEPYL PPKTH
Length:425
Mass (Da):47,291
Last modified:November 8, 2005 - v1
Checksum:iB063850A1FF392AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58777.1
BA000007 Genomic DNA Translation: BAB37931.1
PIRiD98192
RefSeqiNP_312535.1, NC_002695.1
WP_000891564.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58777; AAG58777; Z5057
BAB37931; BAB37931; BAB37931
GeneIDi915541
KEGGiece:Z5057
ecs:ECs4508
PATRICifig|386585.9.peg.4724

Similar proteinsi

Entry informationi

Entry nameiKDTA_ECO57
AccessioniPrimary (citable) accession number: P0AC77
Secondary accession number(s): P23282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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