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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.2 Publications

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications

Enzyme regulationi

Catalytic activity is inhibited by the antibiotic polymixin B and by Re endotoxin.1 Publication

Kineticsi

  1. KM=52 µM for lipid IV(A) (at pH 8)1 Publication
  2. KM=88 µM for CMP-Kdo (at pH 8)1 Publication
  1. Vmax=18 µmol/min/mg enzyme (at pH 8)1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Pathwayi: KDO(2)-lipid A biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  2. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  3. Lipid A biosynthesis lauroyltransferase (lpxL)
  4. Lipid A biosynthesis myristoyltransferase (lpxM)
This subpathway is part of the pathway KDO(2)-lipid A biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601Proton acceptorBy similarity
Sitei130 – 1301Transition state stabilizerBy similarity
Sitei208 – 2081Transition state stabilizerBy similarity

GO - Molecular functioni

  • transferase activity Source: EcoCyc

GO - Biological processi

  • Kdo2-lipid A biosynthetic process Source: UniProtKB-UniPathway
  • lipid A biosynthetic process Source: EcoliWiki
  • lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:KDOTRANS-MONOMER.
ECOL316407:JW3608-MONOMER.
MetaCyc:KDOTRANS-MONOMER.
BRENDAi2.4.99.12. 2026.
2.4.99.13. 2026.
UniPathwayiUPA00360; UER00483.
UPA00360; UER00484.
UPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.122 Publications, EC:2.4.99.132 Publications)
Short name:
Kdo transferase
Alternative name(s):
Bifunctional Kdo transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:kdtA
Ordered Locus Names:b3633, JW3608
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10520. waaA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication; Cytoplasmic side 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Signal-anchorSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene display growth defects, absence of Kdo transferase activity, and accumulate massive amounts of lipid IV(A).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4254253-deoxy-D-manno-octulosonic acid transferasePRO_0000080286Add
BLAST

Post-translational modificationi

Degraded by the protease FtsH; therefore FtsH regulates the addition of the sugar moiety to the LPS and thus the maturation of the LPS precursor.1 Publication

Proteomic databases

PaxDbiP0AC75.

Interactioni

Protein-protein interaction databases

BioGridi4263240. 348 interactions.
DIPiDIP-48036N.
IntActiP0AC75. 16 interactions.
MINTiMINT-1234738.
STRINGi511145.b3633.

Structurei

3D structure databases

ProteinModelPortaliP0AC75.
SMRiP0AC75. Positions 49-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 2692CMP-Kdo bindingBy similarity
Regioni309 – 3113CMP-Kdo bindingBy similarity
Regioni335 – 3384CMP-Kdo bindingBy similarity

Domaini

The N-terminal half of KdtA is responsible for determining the number of Kdo residues that are transferred to lipid IVA.1 Publication

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8A. Bacteria.
COG1519. LUCA.
HOGENOMiHOG000257156.
InParanoidiP0AC75.
KOiK02527.
OMAiKHGGHNP.
PhylomeDBiP0AC75.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF04413. Glycos_transf_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELLYTALL YLIQPLIWIR LWVRGRKAPA YRKRWGERYG FYRHPLKPGG
60 70 80 90 100
IMLHSVSVGE TLAAIPLVRA LRHRYPDLPI TVTTMTPTGS ERVQSAFGKD
110 120 130 140 150
VQHVYLPYDL PDALNRFLNK VDPKLVLIME TELWPNLIAA LHKRKIPLVI
160 170 180 190 200
ANARLSARSA AGYAKLGKFV RRLLRRITLI AAQNEEDGAR FVALGAKNNQ
210 220 230 240 250
VTVTGSLKFD ISVTPQLAAK AVTLRRQWAP HRPVWIATST HEGEESVVIA
260 270 280 290 300
AHQALLQQFP NLLLILVPRH PERFPDAINL VRQAGLSYIT RSSGEVPSTS
310 320 330 340 350
TQVVVGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP
360 370 380 390 400
HTFNFKDICA RLEQASGLIT VTDATTLAKE VSSLLTDADY RSFYGRHAVE
410 420
VLYQNQGALQ RLLQLLEPYL PPKTH
Length:425
Mass (Da):47,291
Last modified:November 8, 2005 - v1
Checksum:iB063850A1FF392AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60670 Genomic DNA. Translation: AAA24043.1.
M86305 Genomic DNA. Translation: AAA03745.1.
U00039 Genomic DNA. Translation: AAB18610.1.
U00096 Genomic DNA. Translation: AAC76657.1.
AP009048 Genomic DNA. Translation: BAE77659.1.
PIRiJU0467.
RefSeqiNP_418090.1. NC_000913.3.
WP_000891564.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76657; AAC76657; b3633.
BAE77659; BAE77659; BAE77659.
GeneIDi949048.
KEGGiecj:JW3608.
eco:b3633.
PATRICi32122753. VBIEscCol129921_3753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60670 Genomic DNA. Translation: AAA24043.1.
M86305 Genomic DNA. Translation: AAA03745.1.
U00039 Genomic DNA. Translation: AAB18610.1.
U00096 Genomic DNA. Translation: AAC76657.1.
AP009048 Genomic DNA. Translation: BAE77659.1.
PIRiJU0467.
RefSeqiNP_418090.1. NC_000913.3.
WP_000891564.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AC75.
SMRiP0AC75. Positions 49-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263240. 348 interactions.
DIPiDIP-48036N.
IntActiP0AC75. 16 interactions.
MINTiMINT-1234738.
STRINGi511145.b3633.

Proteomic databases

PaxDbiP0AC75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76657; AAC76657; b3633.
BAE77659; BAE77659; BAE77659.
GeneIDi949048.
KEGGiecj:JW3608.
eco:b3633.
PATRICi32122753. VBIEscCol129921_3753.

Organism-specific databases

EchoBASEiEB0515.
EcoGeneiEG10520. waaA.

Phylogenomic databases

eggNOGiENOG4105D8A. Bacteria.
COG1519. LUCA.
HOGENOMiHOG000257156.
InParanoidiP0AC75.
KOiK02527.
OMAiKHGGHNP.
PhylomeDBiP0AC75.

Enzyme and pathway databases

UniPathwayiUPA00360; UER00483.
UPA00360; UER00484.
UPA00958.
BioCyciEcoCyc:KDOTRANS-MONOMER.
ECOL316407:JW3608-MONOMER.
MetaCyc:KDOTRANS-MONOMER.
BRENDAi2.4.99.12. 2026.
2.4.99.13. 2026.

Miscellaneous databases

PROiP0AC75.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF04413. Glycos_transf_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKDTA_ECOLI
AccessioniPrimary (citable) accession number: P0AC75
Secondary accession number(s): P23282, Q2M7U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.