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Protein

Glutaredoxin-4

Gene

grxD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Glutathione1 Publication
Metal bindingi30 – 301Iron-sulfur (2Fe-2S); shared with dimeric partner
Binding sitei59 – 591Glutathione1 Publication
Binding sitei71 – 711Glutathione; via amide nitrogen and carbonyl oxygen1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12181-MONOMER.
ECOL316407:JW1646-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-4
Short name:
Grx4
Alternative name(s):
Monothiol glutaredoxin
Gene namesi
Name:grxD
Synonyms:ydhD
Ordered Locus Names:b1654, JW1646
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12181. grxD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 115115Glutaredoxin-4PRO_0000102257Add
BLAST

Proteomic databases

EPDiP0AC69.
PaxDbiP0AC69.
PRIDEiP0AC69.

2D gel databases

SWISS-2DPAGEP0AC69.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
bolAP0ABE22EBI-545828,EBI-545774

Protein-protein interaction databases

BioGridi4260269. 433 interactions.
DIPiDIP-11729N.
IntActiP0AC69. 18 interactions.
MINTiMINT-1258030.
STRINGi511145.b1654.

Structurei

Secondary structure

1
115
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi16 – 238Combined sources
Beta strandi25 – 306Combined sources
Helixi31 – 4111Combined sources
Beta strandi48 – 514Combined sources
Helixi52 – 543Combined sources
Helixi56 – 6611Combined sources
Beta strandi67 – 704Combined sources
Beta strandi73 – 764Combined sources
Beta strandi79 – 835Combined sources
Helixi84 – 929Combined sources
Helixi95 – 10713Combined sources
Beta strandi108 – 1103Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YKANMR-A1-115[»]
2WCIX-ray1.90A/B1-115[»]
ProteinModelPortaliP0AC69.
SMRiP0AC69. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC69.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 107103GlutaredoxinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 852Glutathione binding

Sequence similaritiesi

Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105M2J. Bacteria.
COG0278. LUCA.
HOGENOMiHOG000095211.
InParanoidiP0AC69.
KOiK07390.
OMAiHEFIDNE.
PhylomeDBiP0AC69.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT_like.
IPR014434. Monothiol_GRX.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PIRSFiPIRSF005894. Monothiol_GRX. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTIEKIQR QIAENPILLY MKGSPKLPSC GFSAQAVQAL AACGERFAYV
60 70 80 90 100
DILQNPDIRA ELPKYANWPT FPQLWVDGEL VGGCDIVIEM YQRGELQQLI
110
KETAAKYKSE EPDAE
Length:115
Mass (Da):12,879
Last modified:November 8, 2005 - v1
Checksum:i254B540430632645
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74726.1.
AP009048 Genomic DNA. Translation: BAA15420.1.
L01622 Genomic DNA. Translation: AAC37010.1.
PIRiH64922.
RefSeqiNP_416171.1. NC_000913.3.
WP_000108172.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74726; AAC74726; b1654.
BAA15420; BAA15420; BAA15420.
GeneIDi946169.
KEGGiecj:JW1646.
eco:b1654.
PATRICi32118610. VBIEscCol129921_1725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74726.1.
AP009048 Genomic DNA. Translation: BAA15420.1.
L01622 Genomic DNA. Translation: AAC37010.1.
PIRiH64922.
RefSeqiNP_416171.1. NC_000913.3.
WP_000108172.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YKANMR-A1-115[»]
2WCIX-ray1.90A/B1-115[»]
ProteinModelPortaliP0AC69.
SMRiP0AC69. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260269. 433 interactions.
DIPiDIP-11729N.
IntActiP0AC69. 18 interactions.
MINTiMINT-1258030.
STRINGi511145.b1654.

2D gel databases

SWISS-2DPAGEP0AC69.

Proteomic databases

EPDiP0AC69.
PaxDbiP0AC69.
PRIDEiP0AC69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74726; AAC74726; b1654.
BAA15420; BAA15420; BAA15420.
GeneIDi946169.
KEGGiecj:JW1646.
eco:b1654.
PATRICi32118610. VBIEscCol129921_1725.

Organism-specific databases

EchoBASEiEB2098.
EcoGeneiEG12181. grxD.

Phylogenomic databases

eggNOGiENOG4105M2J. Bacteria.
COG0278. LUCA.
HOGENOMiHOG000095211.
InParanoidiP0AC69.
KOiK07390.
OMAiHEFIDNE.
PhylomeDBiP0AC69.

Enzyme and pathway databases

BioCyciEcoCyc:EG12181-MONOMER.
ECOL316407:JW1646-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AC69.
PROiP0AC69.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT_like.
IPR014434. Monothiol_GRX.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PIRSFiPIRSF005894. Monothiol_GRX. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX4_ECOLI
AccessioniPrimary (citable) accession number: P0AC69
Secondary accession number(s): P37010, P77424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.