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Protein

Glutaredoxin-like protein NrdH

Gene

nrdH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Electron transport system for the ribonucleotide reductase system NrdEF.

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  • cell redox homeostasis Source: EcoCyc
  • positive regulation of oxidoreductase activity Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7401-MONOMER.
ECOL316407:JW2648-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-like protein NrdH
Gene namesi
Name:nrdH
Synonyms:ygaN
Ordered Locus Names:b2673, JW2648
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13286. nrdH.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Glutaredoxin-like protein NrdHPRO_0000141638Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi11 ↔ 14Redox-active

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AC65.

Expressioni

Inductioni

Induced 2-fold by hydroxyurea.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260693. 11 interactions.
DIPiDIP-48164N.
IntActiP0AC65. 7 interactions.
STRINGi511145.b2673.

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi12 – 2312Combined sources
Beta strandi29 – 324Combined sources
Turni33 – 353Combined sources
Helixi37 – 459Combined sources
Beta strandi53 – 564Combined sources
Beta strandi59 – 635Combined sources
Helixi66 – 694Combined sources
Helixi70 – 723Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H75X-ray1.70A1-81[»]
ProteinModelPortaliP0AC65.
SMRiP0AC65. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8181GlutaredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutaredoxin family.Curated
Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105WAZ. Bacteria.
COG0695. LUCA.
HOGENOMiHOG000095206.
InParanoidiP0AC65.
KOiK06191.
OMAiEQHWAGF.
OrthoDBiEOG6Z3KS6.
PhylomeDBiP0AC65.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR011909. GlrX_NrdH.
IPR002109. Glutaredoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02194. GlrX_NrdH. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRITIYTRND CVQCHATKRA MENRGFDFEM INVDRVPEAA EALRAQGFRQ
60 70 80
LPVVIAGDLS WSGFRPDMIN RLHPAPHAAS A
Length:81
Mass (Da):9,139
Last modified:November 8, 2005 - v1
Checksum:i6D0878E9CD133137
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79787 Genomic DNA. Translation: CAA56184.1.
U00096 Genomic DNA. Translation: AAC75720.1.
AP009048 Genomic DNA. Translation: BAE76782.1.
PIRiS70891.
RefSeqiNP_417159.1. NC_000913.3.
WP_001223227.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75720; AAC75720; b2673.
BAE76782; BAE76782; BAE76782.
GeneIDi947161.
KEGGiecj:JW2648.
eco:b2673.
PATRICi32120736. VBIEscCol129921_2765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79787 Genomic DNA. Translation: CAA56184.1.
U00096 Genomic DNA. Translation: AAC75720.1.
AP009048 Genomic DNA. Translation: BAE76782.1.
PIRiS70891.
RefSeqiNP_417159.1. NC_000913.3.
WP_001223227.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H75X-ray1.70A1-81[»]
ProteinModelPortaliP0AC65.
SMRiP0AC65. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260693. 11 interactions.
DIPiDIP-48164N.
IntActiP0AC65. 7 interactions.
STRINGi511145.b2673.

Proteomic databases

PaxDbiP0AC65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75720; AAC75720; b2673.
BAE76782; BAE76782; BAE76782.
GeneIDi947161.
KEGGiecj:JW2648.
eco:b2673.
PATRICi32120736. VBIEscCol129921_2765.

Organism-specific databases

EchoBASEiEB3071.
EcoGeneiEG13286. nrdH.

Phylogenomic databases

eggNOGiENOG4105WAZ. Bacteria.
COG0695. LUCA.
HOGENOMiHOG000095206.
InParanoidiP0AC65.
KOiK06191.
OMAiEQHWAGF.
OrthoDBiEOG6Z3KS6.
PhylomeDBiP0AC65.

Enzyme and pathway databases

BioCyciEcoCyc:G7401-MONOMER.
ECOL316407:JW2648-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AC65.
PROiP0AC65.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR011909. GlrX_NrdH.
IPR002109. Glutaredoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02194. GlrX_NrdH. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria."
    Jordan A., Aragall E., Gibert I., Barbe J.
    Mol. Microbiol. 19:777-790(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  5. "Structural basis for the thioredoxin-like activity profile of the glutaredoxin-like NrdH-redoxin from Escherichia coli."
    Stehr M., Schneider G., Aslund F., Holmgren A., Lindqvist Y.
    J. Biol. Chem. 276:35836-35841(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiNRDH_ECOLI
AccessioniPrimary (citable) accession number: P0AC65
Secondary accession number(s): Q2MAC4, Q47414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: July 6, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.