Reviewed, UniProtKB/Swiss-Prot P0AC61 (GLRX2_ECO57)

Names and origin

Protein names

Recommended name:
    Glutaredoxin-2
      Short name=Grx2

Gene names

Name:	grxB
Ordered Locus Names:	Z1701, ECs1442

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in reducing some disulfides in a coupled system with glutathione reductase. Does not act as hydrogen donor for ribonucleotide reductase By similarity.
Sequence similarities	Belongs to the glutaredoxin family. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Glutaredoxin-2		PRO_0000141586
Regions
	Domain	1 – 77	77	GST N-terminal
Amino acid modifications
	Disulfide bond	9 ↔ 12		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AC61-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: A8AAAB2548D824FE	FASTA	215	24,350
	10 20 30 40 50 60 MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP 70 80 90 100 110 120 ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK 130 140 150 160 170 180 YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR 190 200 210 NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG55810.1. BA000007 Genomic DNA. Translation: BAB34865.1.
PIR	B99809. F85668.
RefSeq	NP_287198.1. NP_309469.1.
3D structure databases
SMR	P0AC61. Positions 1-215.
ModBase	Search...
Genome annotation databases
GeneID	912384. 959403.
GenomeReviews	Gene locus Z1701 in contig AE005174_GR. Gene locus ECs1442 in contig BA000007_GR.
KEGG	ece:Z1701. ecs:ECs1442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AC61.
OMA	P0AC61. KMAPILQ.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS1442-MON.
Family and domain databases
InterPro	IPR011767. GLR_AS. IPR007494. Glutaredoxin2_C. IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR011901. GRXB. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF04399. Glutaredoxin2_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02182. GRXB. 1 hit.
PROSITE	PS00195. GLUTAREDOXIN_1. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLRX2_ECO57

Accession

Primary (citable) accession number: P0AC61
Secondary accession number(s): P39811, P75928, P77043

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)