Glutaredoxin-2 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AC59 (GLRX2_ECOLI)

Last modified June 16, 2009. Version 34. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Glutaredoxin-2
Short name=Grx2

Gene names

Name:	grxB
Ordered Locus Names:	b1064, JW1051

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

Involved in reducing some disulfides in a coupled system with glutathione reductase. Does not act as hydrogen donor for ribonucleotide reductase.

Sequence similarities

Belongs to the glutaredoxin family.

Contains 1 GST N-terminal domain.

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Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Redox-active center
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 215

215

Glutaredoxin-2

PRO_0000141585

Regions

Domain

1 – 77

GST N-terminal

Amino acid modifications

Disulfide bond

9 ↔ 12

Redox-active By similarity

Experimental info

Sequence conflict

L → I AA sequence Ref.5

Secondary structure

215

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AC59-1 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: A8AAAB2548D824FE
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FASTA

215

24,350

        10         20         30         40         50         60 
MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP 

        70         80         90        100        110        120 
ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK 

       130        140        150        160        170        180 
YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR 

       190        200        210 
NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, overexpression, and characterization of glutaredoxin 2, an atypical glutaredoxin from Escherichia coli." Vlamis-Gardikas A., Aaslund F., Spyrou G., Bergman T., Holmgren A. J. Biol. Chem. 272:11236-11243(1997) [PubMed: 9111025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Two additional glutaredoxins exist in Escherichia coli: glutaredoxin 3 is a hydrogen donor for ribonucleotide reductase in a thioredoxin/glutaredoxin 1 double mutant." Aaslund F., Ehn B., Miranda-Vizuete A., Pueyo C., Holmgren A. Proc. Natl. Acad. Sci. U.S.A. 91:9813-9817(1994) [PubMed: 7937896] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18, CHARACTERIZATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X92076 Genomic DNA. Translation: CAA63058.1.
U00096 Genomic DNA. Translation: AAC74148.1.
AP009048 Genomic DNA. Translation: BAA35872.1.

PIR

E64849.

RefSeq

AP_001690.1.
NP_415582.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G7O	NMR	-	A	1-215	[»]

ModBase

Search...

Genome annotation databases

GeneID

946926.

GenomeReviews

Gene locus JW1051 in contig AP009048_GR.
Gene locus b1064 in contig U00096_GR.

KEGG

ecj:JW1051.
eco:b1064.

Organism-specific databases

EchoBASE

EB2551.

EcoGene

EG12688. grxB.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AC59.

OMA

P0AC59. KMAPILQ.

Enzyme and pathway databases

BioCyc

EcoCyc:GRXB-MON.

Family and domain databases

InterPro

IPR011767. GLR_AS.
IPR007494. Glutaredoxin2_C.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR011901. GRXB.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF04399. Glutaredoxin2_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02182. GRXB. 1 hit.

PROSITE

PS00195. GLUTAREDOXIN_1. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

GLRX2_ECOLI

Accession

Primary (citable) accession number: P0AC59
Secondary accession number(s): P39811, P75928, P77043

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families