P0AC58 (GLNK_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein P-II 2

Gene names

Name:	glnK
Ordered Locus Names:	SF0395, S0401

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by glnE, so activating the enzyme By similarity.
Subunit structure	Homotrimer By similarity.
Post-translational modification	Uridylylated/deuridylylated by glnD By similarity. Uridylylated By similarity.
Sequence similarities	Belongs to the P(II) protein family.
Sequence caution	The sequence AAN42051.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of nitrogen utilization Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Nitrogen regulatory protein P-II 2		PRO_0000139792
Amino acid modifications
	Modified residue	51	1	O-UMP-tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AC58 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 1875CD92C162B537	FASTA	112	12,259
	10 20 30 40 50 60 MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE YSVNFLPKVK 70 80 90 100 110 IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI RIRTGEADEA AL

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN42051.1. Different initiation. AE014073 Genomic DNA. Translation: AAP15928.1.
RefSeq	NP_706344.2. NC_004337.2. NP_836122.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AC58.
SMR	P0AC58. Positions 1-112.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000085318; EBESCP00000082140; EBESCG00000084364. EBESCT00000091969; EBESCP00000088605; EBESCG00000091013.
GeneID	1027682. 1076841.
GenomeReviews	Gene locus SF0395 in contig AE005674_GR. Gene locus S0401 in contig AE014073_GR.
KEGG	sfl:SF0395. sfx:S0401.
PATRIC	18701821. VBIShiFle31049_0443.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008988.
HOGENOM	HBG748089.
ProtClustDB	PRK10665.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN42051.1-MONOMER.
Family and domain databases
InterPro	IPR002187. N-reg_PII. IPR011322. N-reg_PII-like_a/b. IPR015867. N-reg_PII/ATP_PRibTrfase_C. IPR017918. N-reg_PII_CS. IPR002332. N-reg_PII_urydylation_site. [Graphical view]
Gene3D	G3DSA:3.30.70.120. PII_glnB. 1 hit.
KO	K04752.
Pfam	PF00543. P-II. 1 hit. [Graphical view]
PRINTS	PR00340. PIIGLNB.
SMART	SM00938. P-II. 1 hit. [Graphical view]
SUPFAM	SSF54913. N-reg_PII-like_a/b. 1 hit.
PROSITE	PS00638. PII_GLNB_CTER. 1 hit. PS51343. PII_GLNB_DOM. 1 hit. PS00496. PII_GLNB_UMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNK_SHIFL

Accession

Primary (citable) accession number: P0AC58
Secondary accession number(s): P38504, P77118

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program