Nitrogen regulatory protein P-II 2 - Escherichia coli (strain K12)

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P0AC55 (GLNK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein P-II 2

Gene names

Name:	glnK
Synonyms:	ybaI
Ordered Locus Names:	b0450, JW0440

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by glnE, so activating the enzyme By similarity.
Subunit structure	Homotrimer.
Post-translational modification	Uridylylated/deuridylylated by glnD. Uridylylated.
Sequence similarities	Belongs to the P(II) protein family.
Sequence caution	The sequence AAB40206.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of nitrogen utilization Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
glnL	P0AFB5	4	EBI-559503,EBI-701156

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 112

112

Nitrogen regulatory protein P-II 2

PRO_0000139772

Amino acid modifications

Modified residue

O-UMP-tyrosine By similarity

Secondary structure

112

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AC55 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 1875CD92C162B537
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FASTA

112

12,259

        10         20         30         40         50         60 
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE YSVNFLPKVK 

        70         80         90        100        110 
IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI RIRTGEADEA AL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An additional PII in Escherichia coli: a new regulatory protein in the glutamine synthetase cascade." Vanheeswijk W.C., Stegeman B., Hoving S., Molenaar D., Kahn D., Westerhoff H.V. FEMS Microbiol. Lett. 132:153-157(1995) [PubMed: 7590157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance." Allikmets R., Gerrard B.C., Court D., Dean M.C. Gene 136:231-236(1993) [PubMed: 7904973] [Abstract] Cited for: IDENTIFICATION.
[6]	"An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli." van Heeswijk W.C., Hoving S., Molenaar D., Stegeman B., Kahn D., Westerhoff H.V. Mol. Microbiol. 21:133-146(1996) [PubMed: 8843440] [Abstract] Cited for: CHARACTERIZATION.
[7]	"GlnK, a PII-homologue: structure reveals ATP binding site and indicates how the T-loops may be involved in molecular recognition." Xu Y., Cheah E., Carr P.D., van Heeswijk W.C., Westerhoff H.V., Vasudevan S.G., Ollis D.L. J. Mol. Biol. 282:149-165(1998) [PubMed: 9733647] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U40429 Genomic DNA. Translation: AAD14836.1.
S79842 Genomic DNA. Translation: AAA87955.1.
U82664 Genomic DNA. Translation: AAB40206.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73553.1.
AP009048 Genomic DNA. Translation: BAE76230.1.

PIR

B64775.

RefSeq

NP_414984.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GNK	X-ray	2.00	A/B	1-112	[»]
2GNK	X-ray	2.00	A	1-112	[»]
2NS1	X-ray	1.96	B	1-112	[»]
2NUU	X-ray	2.50	G/H/I/J/K/L	1-112	[»]

ProteinModelPortal

P0AC55.

SMR

P0AC55. Positions 1-112.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35006N.

IntAct

P0AC55. 14 interactions.

MINT

MINT-1309225.

2D gel databases

SWISS-2DPAGE

P0AC55.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000557; EBESCP00000000557; EBESCG00000000466.
EBESCT00000015336; EBESCP00000014627; EBESCG00000014396.

GeneID

945087.

GenomeReviews

Gene locus JW0440 in contig AP009048_GR.
Gene locus b0450 in contig U00096_GR.

KEGG

ecj:JW0440.
eco:b0450.

PATRIC

32116055. VBIEscCol129921_0469.

Organism-specific databases

EchoBASE

EB2108.

EcoGene

EG12191. glnK.

Phylogenomic databases

eggNOG

COG0347.

GeneTree

EBGT00050000008988.

HOGENOM

HBG748089.

OMA

IEAITRI.

PhylomeDB

P0AC55.

ProtClustDB

PRK10665.

Enzyme and pathway databases

BioCyc

EcoCyc:PROTEIN-PII2.

Gene expression databases

Genevestigator

P0AC55.

Family and domain databases

InterPro

IPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]

Gene3D

G3DSA:3.30.70.120. PII_glnB. 1 hit.

K04752.

Pfam

PF00543. P-II. 1 hit.
[Graphical view]

PRINTS

PR00340. PIIGLNB.

SMART

SM00938. P-II. 1 hit.
[Graphical view]

SUPFAM

SSF54913. N-reg_PII-like_a/b. 1 hit.

PROSITE

PS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GLNK_ECOLI

Accession

Primary (citable) accession number: P0AC55
Secondary accession number(s): P38504, P77118, Q2MBX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents