Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogen regulatory protein P-II 2

Gene

glnK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functioni

  • enzyme regulator activity Source: InterPro
  • identical protein binding Source: IntAct
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of nitrogen utilization Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processTranscription, Transcription regulation
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II 2
Gene namesi
Name:glnK
Synonyms:ybaI
Ordered Locus Names:b0450, JW0440
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG12191 glnK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397721 – 112Nitrogen regulatory protein P-II 2Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51O-UMP-tyrosinePROSITE-ProRule annotation1

Post-translational modificationi

Uridylylated/deuridylylated by GlnD.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AC55
PRIDEiP0AC55

2D gel databases

SWISS-2DPAGEiP0AC55

Interactioni

Subunit structurei

Homotrimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263149, 10 interactors
DIPiDIP-35006N
IntActiP0AC55, 14 interactors
STRINGi316385.ECDH10B_0406

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 8Combined sources8
Helixi10 – 12Combined sources3
Helixi13 – 22Combined sources10
Beta strandi29 – 35Combined sources7
Beta strandi37 – 39Combined sources3
Beta strandi44 – 46Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi56 – 66Combined sources11
Helixi67 – 69Combined sources3
Helixi70 – 81Combined sources12
Beta strandi84 – 86Combined sources3
Beta strandi90 – 97Combined sources8
Turni102 – 104Combined sources3
Helixi108 – 111Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNKX-ray2.00A/B1-112[»]
2GNKX-ray2.00A1-112[»]
2NS1X-ray1.96B1-112[»]
2NUUX-ray2.50G/H/I/J/K/L1-112[»]
ProteinModelPortaliP0AC55
SMRiP0AC55
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC55

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA Bacteria
COG0347 LUCA
HOGENOMiHOG000017847
InParanoidiP0AC55
KOiK04752
OMAiAIIRMER
PhylomeDBiP0AC55

Family and domain databases

Gene3Di3.30.70.120, 1 hit
InterProiView protein in InterPro
IPR002187 N-reg_PII
IPR011322 N-reg_PII-like_a/b
IPR015867 N-reg_PII/ATP_PRibTrfase_C
IPR017918 N-reg_PII_CS
IPR002332 N-reg_PII_urydylation_site
PANTHERiPTHR30115 PTHR30115, 1 hit
PfamiView protein in Pfam
PF00543 P-II, 1 hit
PIRSFiPIRSF039144 GlnB, 1 hit
PRINTSiPR00340 PIIGLNB
SMARTiView protein in SMART
SM00938 P-II, 1 hit
SUPFAMiSSF54913 SSF54913, 1 hit
PROSITEiView protein in PROSITE
PS00638 PII_GLNB_CTER, 1 hit
PS51343 PII_GLNB_DOM, 1 hit
PS00496 PII_GLNB_UMP, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE
60 70 80 90 100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI
110
RIRTGEADEA AL
Length:112
Mass (Da):12,259
Last modified:November 8, 2005 - v1
Checksum:i1875CD92C162B537
GO

Sequence cautioni

The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA Translation: AAD14836.1
S79842 Genomic DNA Translation: AAA87955.1
U82664 Genomic DNA Translation: AAB40206.1 Different initiation.
U00096 Genomic DNA Translation: AAC73553.1
AP009048 Genomic DNA Translation: BAE76230.1
PIRiB64775
RefSeqiNP_414984.1, NC_000913.3
WP_000780338.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73553; AAC73553; b0450
BAE76230; BAE76230; BAE76230
GeneIDi945087
KEGGiecj:JW0440
eco:b0450
PATRICifig|1411691.4.peg.1825

Entry informationi

Entry nameiGLNK_ECOLI
AccessioniPrimary (citable) accession number: P0AC55
Secondary accession number(s): P38504, P77118, Q2MBX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health