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Protein

Nitrogen regulatory protein P-II 2

Gene

glnK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of nitrogen utilization Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII2.
ECOL316407:JW0440-MONOMER.
MetaCyc:PROTEIN-PII2.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II 2
Gene namesi
Name:glnK
Synonyms:ybaI
Ordered Locus Names:b0450, JW0440
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12191. glnK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397721 – 112Nitrogen regulatory protein P-II 2Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51O-UMP-tyrosinePROSITE-ProRule annotation1

Post-translational modificationi

Uridylylated/deuridylylated by GlnD.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AC55.
PRIDEiP0AC55.

2D gel databases

SWISS-2DPAGEP0AC55.

Interactioni

Subunit structurei

Homotrimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-559503,EBI-559503
glnBP0A9Z13EBI-559503,EBI-551053
glnDP272493EBI-559503,EBI-552032
glnLP0AFB55EBI-559503,EBI-701156

Protein-protein interaction databases

BioGridi4263149. 10 interactors.
DIPiDIP-35006N.
IntActiP0AC55. 14 interactors.
MINTiMINT-1309225.
STRINGi511145.b0450.

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 8Combined sources8
Helixi10 – 12Combined sources3
Helixi13 – 22Combined sources10
Beta strandi29 – 35Combined sources7
Beta strandi37 – 39Combined sources3
Beta strandi44 – 46Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi56 – 66Combined sources11
Helixi67 – 69Combined sources3
Helixi70 – 81Combined sources12
Beta strandi84 – 86Combined sources3
Beta strandi90 – 97Combined sources8
Turni102 – 104Combined sources3
Helixi108 – 111Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNKX-ray2.00A/B1-112[»]
2GNKX-ray2.00A1-112[»]
2NS1X-ray1.96B1-112[»]
2NUUX-ray2.50G/H/I/J/K/L1-112[»]
ProteinModelPortaliP0AC55.
SMRiP0AC55.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC55.

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiP0AC55.
KOiK04752.
OMAiGGHKEVY.
PhylomeDBiP0AC55.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AC55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE
60 70 80 90 100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI
110
RIRTGEADEA AL
Length:112
Mass (Da):12,259
Last modified:November 8, 2005 - v1
Checksum:i1875CD92C162B537
GO

Sequence cautioni

The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14836.1.
S79842 Genomic DNA. Translation: AAA87955.1.
U82664 Genomic DNA. Translation: AAB40206.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73553.1.
AP009048 Genomic DNA. Translation: BAE76230.1.
PIRiB64775.
RefSeqiNP_414984.1. NC_000913.3.
WP_000780338.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73553; AAC73553; b0450.
BAE76230; BAE76230; BAE76230.
GeneIDi945087.
KEGGiecj:JW0440.
eco:b0450.
PATRICi32116055. VBIEscCol129921_0469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA. Translation: AAD14836.1.
S79842 Genomic DNA. Translation: AAA87955.1.
U82664 Genomic DNA. Translation: AAB40206.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73553.1.
AP009048 Genomic DNA. Translation: BAE76230.1.
PIRiB64775.
RefSeqiNP_414984.1. NC_000913.3.
WP_000780338.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNKX-ray2.00A/B1-112[»]
2GNKX-ray2.00A1-112[»]
2NS1X-ray1.96B1-112[»]
2NUUX-ray2.50G/H/I/J/K/L1-112[»]
ProteinModelPortaliP0AC55.
SMRiP0AC55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263149. 10 interactors.
DIPiDIP-35006N.
IntActiP0AC55. 14 interactors.
MINTiMINT-1309225.
STRINGi511145.b0450.

2D gel databases

SWISS-2DPAGEP0AC55.

Proteomic databases

PaxDbiP0AC55.
PRIDEiP0AC55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73553; AAC73553; b0450.
BAE76230; BAE76230; BAE76230.
GeneIDi945087.
KEGGiecj:JW0440.
eco:b0450.
PATRICi32116055. VBIEscCol129921_0469.

Organism-specific databases

EchoBASEiEB2108.
EcoGeneiEG12191. glnK.

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiP0AC55.
KOiK04752.
OMAiGGHKEVY.
PhylomeDBiP0AC55.

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII2.
ECOL316407:JW0440-MONOMER.
MetaCyc:PROTEIN-PII2.

Miscellaneous databases

EvolutionaryTraceiP0AC55.
PROiP0AC55.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNK_ECOLI
AccessioniPrimary (citable) accession number: P0AC55
Secondary accession number(s): P38504, P77118, Q2MBX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.